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Micro Lecture
Micro Lecture
MICROBIAL GROWTH IN
■pH
FOODS
■Temperature
Why is food considered to be a great
medium to support microbial ■Aeration
growth?
Nutrients
■Food is a good medium that
provides different nutrients and -Nutrients are necessary for
micronutrients to support microbial microbial growth and play a vital
growth at optimum growth role in the proper cultivation of
conditions. microorganisms in the laboratory
and for proper growth in their
–Food is comprised of different natural environments.
ingredients such as
:–Carbohydrates
-The types of nutrients that are
–Proteins required include those that supply
energy, carbon and additional
–Fats
necessary materials. The nutrients
Nutrients used to propagate growth are
organism -specific, based on their
■What are the general growth
cellular and metabolic processes.
requirements needed by
microorganisms:
– Nutrition Carbon sources : is require for
microbial growth
■Carbon source
Nitrogen sources: Nitrogen is a
■Nitrogen Source
major components of proteins,
■Sulfur source nucleic acids, and other compounds
accounting for approximately 5% of
■Phosphorus source the dry weight of a typical bacterial
■Micronutrients cell.
Environmental Factors: pH
■ If microorganisms exposed in
different pH, they regulate their
internal pH by pumping protons in
or out of their cell
Environmental Factors:
Temperature
■ Different microbial species vary Facultative anaerobes - are
widely on organisms that
their optimal temperatures for thrive in the presence of oxygen but
growth. also grow
in its absence by relying on
fermentation or
anaerobic respiration, if there is a
suitable
electron acceptor other than oxygen
and the
organism is able to perform
anaerobic
respiration.
Environmental Factors: Aeration
■ Many microorganisms can be
found in aerobic and
anaerobic environments. Thus,
different
microorganisms have their own
oxygen
requirement to support their growth.
Obligate aerobes – bacteria that Aerotolerant anaerobes – they can
cannot grow
tolerate the presence of oxygen in
without an abundant supply of their
oxygen.
culture environment. Also, they
Obligate anaerobes – bacteria that
cannot capable of producing ATP by
– Extrinsic Parameters
■ Temperature of storage