Electron Transport Chain: A rollercoaster ride that

produces energy
Question 1: If it does transfer energy and the electrons are excited, how will the amount of oxidized
(blue) blue DCPIPD C P I P change in relation to the controls?
✓ Less oxidized (blue) DCPIPD C P I P than the negative control
– More oxidized (blue) DCPIPD C P I P than the positive control
– More oxidized (blue) DCPIPD C P I P than the negative control
– Same amount of oxidized (blue) DCPIPD C P I P as the negative control

Question 2: If it doesn’t transfer energy and the electrons aren’t excited, how will the amount of
oxidized (blue) blue DCPIPD C P I P change in relation to the controls?
✓ Same amount of oxidized (blue) DCPIPD C P I P as the negative control
– Less oxidized (blue) DCPIPD C P I P than the negative control
– More oxidized (blue) DCPIPD C P I P than the positive control
– More oxidized (blue) DCPIPD C P I P than the negative control

Question 3: Roxy: What is your most important finding?

✓ The red pigment can use green light for Photosynthesis
– Phycoerythrobilin has several absorption peaks between 500nm and 600nm
– The pigment absorbs electromagnetic waves with a wavelength of 580 nm
– The HPLC lab identified the pigment as a phycoerythrobilin

Question 4: Where does the light reaction of Photosynthesis take place?

✓ In the thylakoid membrane
– In the plasma membrane
– In the chloroplast lumen
– In the stroma of the chloroplast

Question 5: Where is the light energy converted into an electron flow?

✓ In the reaction center chlorophyll
– Within the NADP+ molecule
– At the ATP-synthase
– At each individual accessory pigment molecule

Question 6: In what order do the electrons move through the electron transport chain?
✓ water - photosystem II - photosystem I - NADPH
– Photosystem I - water - photosystem II - NADPH
– water - photosystem II - NADPH - photosystem I
– water - photosystem I - photosystem II - NADPH

Question 7: What is produced during the light reaction? Choose the correct chemical equation.
✓ 2 H2O + 2 NADP+ + nADP + nPi + hv produce O2 + 2 NADPH + 2 H+ + nATP
– 2 H2O + 2 NADPH + nADP + nPi + hv produce O2 + 2 NADP+ + 2 H+ + nATP
– O2 + 2 NADP+ + nATP + hv produce 2 H2O + 2 NADPH + 2 H+ + nADP + nPi
– 2 H2O + 2 NADP+ + nATP + hv produce O2 + 2 NADPH + 2 H+ + nADP + nPi

Question 8: Where does the electron flow that we measure with DCPIPD C P I P originate?
✓ Photosystem II
– Cytochrome
– ATP-Synthase
– Photosystem I

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Question 9: What would be the best negative control?

Remember that a negative control should NOT show change during the experiment.
✓ Keep a sample in the dark
– Use the herbicide DCMU to block the photosystem
– Boil the solution
– Don't add any DCPIP

Question 10: What would be a good positive control for this experiment?

Remember that a positive control should show a change during the experiment.
✓ Expose a sample to the normal light spectrum
– Add a molecule that acts as reducing agent
– Open the lid of the cuvette
– Keep a sample in the dark

Question 11: Click on the VIEW IMAGE button to see the absorbance curves for hydrophobic and
hydrophilic pigments.
Since we know that hydrophobic chlorophylls (in the hexane extract)in the hexane extract don’t
absorb green light, we are interested in the hydrophilic pigments (in the water extract)in the water
extract.

Looking at the water extract, what wavelength should we use to illuminate the treatment sample?
✓ 580 nm
– 410 nm
– 690 nm
– 450 nm

Question 12: Why do we use DCMUD C M U?

✓ We avoid further reduction of DCPIP under the lab light
– It binds and ihibits photosystem I
– It blocks all redox reactions in the chloroplast
– The herbicide kills the algae

Question 13: Now it’s time to measure the reduction of DCPIP through the change in blue color.
To do this, we will shine a specific wavelength on the samples that should be absorbed by the blue
DCPIP but not by the pigments in the solution. That way, we can quantify the decrease in blue color
with a change in absorbance.

Looking at the absorbance spectrum in the VIEW IMAGE button, what wavelength should we use?
✓ 610 nm
– 580 nm
– 510 nm
– 450 nm

Question 14: Compare the values obtained for the three samples. Was your previous hypothesis
right? Is the red pigment really a photosynthetic pigment?
✓ Yes, because photosystem II was active in green light
– No, because photosystem II was active in green light
– No, because photosystem II was inactive in green light
– Yes, because photosystem II was inactive in green light

Question 15: Compare the values obtained for the three samples. Was your previous hypothesis
right? Is the red pigment really a photosynthetic pigment?
✓ No my hypothesis was wrong, because photosystem II was active in green light.

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– Yes my hypothesis was right, because photosystem II was inactive in green light
– Yes my hypothesis was right, because photosystem II was active in green light
– No my hypothesis was wrong, because photosystem II was inactive in green light

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