Republic of the Philippines

CAVITE STATE UNIVERSITY

College of Criminal Justice
Don Severino Delas Alas Campus, Indang, Cavite

NAME: _________BAUER, JOHN VINCENT M. _______

DATE:______________________________________
YEAR & SECTION: _________BSC3-3 _________

ACTIVITY NO. 2
THE MICROSCOPE

I. OBJECTIVE

To familiarize the different parts of the microscope and the function of each part and
how to properly observe a minute specimen under the microscope.

II. MATERIALS

Virtual Microscope

III. PROCUDURE

Follow the set of directions in each section of the lab as you navigate the virtual lab
pages. Record all data and results in the indicated spaces for each lab section.

PART 1. COMPOUND MICROSCOPE PART 1

Go to the following link and answer the questions below.

LINK: Virtual Labs (nmsu.edu)

1. What type of microscope is used in the virtual lab?

 Light Microscope with 10x,40x and 100x lenses.
2. How does this microscope magnify image?
 Works by magnifying the samples on the slide using a series of lenses
3. What does the diaphragm do?
 Adjust the amount of light on the slide.
4. What moves the stage up and down?
 The coarse focus adjustment knob
5. Calculate the total magnification and enter it in the table below for each of the three objective lenses on
this microscope.

OCULAR LENS OBJECTIVE LENS TOTAL MAGNIFICATION

10x 10x 100x

10x 40x 400x

10x 100x 1000x

6. When focusing the microscope, always start with the _______ adjustment.
 Coarse focus
7. When viewing a sample, which lens should you begin with?
 Start with the 10 times objective and place the stage as close to the objective as possible
8. Why is oil used in oil immersion lens?
 Oil prevent light distortion and make the image clearer.
9. How many drops of oil should you use?
 Only add a single drop on the center of the lens.
10. What did you view on the slide?
  Fine focus adjustment
11. What color is gram positive bacteria?
 Purple
12. What color is gram negative bacteria?
 Pink
13. What does it mean if you see pink stains in a gram stained side?
 Meaning that the sample is contaminated
14. Name two gram negative bacteria often found in contaminated food.
 E. Coli or Salmonella
15. How do you clean the oil immersion lens when you have finished with it?
 Wipe the excess oil from the microscope lens by using a piece of lens paper
16. What will sterilize the equipment with high pressure steam?
 Autoclave
17. Were the yoghurt samples contaminated? Yes the yoghurt sample was contaminated

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Republic of the Philippines

CAVITE STATE UNIVERSITY
 College of Criminal Justice
Don Severino Delas Alas Campus, Indang, Cavite

PART 2. PARTS OF THE COMPOUND MICROSCOPE

Label the parts of the microscope below in the corresponding number under the image. Write your answers in
the table provided below.

PART FUNCTION
1. Occular lens(eye This is the part used to look through the microscope. Its found at the top of
piece) the microscope. Its standard magnification is 10x with an optional eyepiece
having magnifications from 5X to 30X.

2. Diopter adjustment Useful as a means to change focus on one eyepiece so as to correct for any
difference in vision between your two eyes.

3. Head The body tube connects the eyepiece to the objective lenses.

4. Nose piece A rotating turret that houses the objective lenses. The viewer spins the
nosepiece to select different objective lenses.

5. Objective lens One of the most important parts of a compound microscope, as they are the
lenses closest to the specimen.

6. Arm(Carrying Handle) The arm connects the body tube to the base of the microscope.

7. Aperture The hole in the middle of the stage that allows light from the illuminator to
reach the specimen.

8. Stage clip Metal clips that hold the slide in place.

9. Mechanical stage The flat platform where the slide is placed

10. Coarse adjustment Brings the specimen into general focus.

11. Diaphragm Adjusts the amount of light that reaches the specimen.

12. Condenser Gathers and focuses light from the illuminator onto the specimen being
viewed

13. Fine adjustment Fine tunes the focus and increases the detail of the specimen.

14. Illuminator The light source for a microscope. Older microscopes used mirrors to reflect
light from an external source up through the bottom of the stage; however,
most microscopes now use a low-voltage bulb.

15. Base The base supports the microscope and it’s where illuminator is located.

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