Ttelation e/ Coltim

Expt. No..
Date
Page No. 4

Airm
Kolatehe colilhm -lauem he
L0aToo ample by Audlaceplale counl
Hechniaue
Kequineaeik
lap oellL2el _ualekansple-
b ie Lanple oil F Colr
Llubel ladlele bagmnCesteL_
CernlainiMa inveeifed
L_Pipettes.

hinciple
Cali oM ae acultaTie ana£Lobic
baclpuin lacteleaml pUOlute
ad_acd Acic PHOdulien a i b e
tocled buenn0CeNLA_pueyple
al
lhe
aL ndlicalal
detectee _hy
am qalel_phacleaclhon
ing înveilea
inveled
uhamk tubel.

PnCeduAL
Mediun porepatilien 2
AinaleAAuenolb
And
2epane
io ul e oen-
double
Signature :
la lake
 Page No.N5
Expt. No.

hounib mecliun b using heollousing

CenopeAitien

Single Ktarerqh
_ adlpke-b qmA
Peptone
Beel_e lua -3qnL
DH-6
r l l e d Lode 1000 al
Duble he inguealienah t o p04epauL
douhleAloength
mealium

* p0Ked10M Lingle Ktuengh

laclote bamopcHelel_ pueple_baDth nto
each efo 1o Hettube.
loeralh.
p e u k e LO M double

locdele ekolpueple_becth întn

lacb e hdest tubtel
Dteunbluce b a b ein_ an inveilec
poRRen in allHest tubek- Staile the
mediun- lacing_a a Cettan plug en
each fest h in Qutoclave
Thubaill he eAltuhel oilh aler
bample in he 15 dektube_al pe
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Expt. No. Page No. 6

be labellina
Jhe testtuba incubaled 3412
o 9 8 - 2 bowd Qno ezamine b e
gal n d mation inalicaldps4Pliel
fat bel
Cankidested
oitbedtany
a negalhve:
9al
Cenlitnnatfon et a
fd lo
pedulian
ad givelhe
2ek umive the poulence Lelldmcl
tlence Cen natoiy Hest_cDal D e L d e d
Lenlft the PHedente Cetrlone
s e p -ull Cultune 9bm a
PAKitiLe_ bel taken_akd dealed
en elin_ meibslene blue aqa a ptubatedd
af 3 24 48 bdual ttollnl
ditind celene _tOih_gHLen_m lale"
Ahealh

b&eatAD,
Dulurmpiiye
L n e To cyello)colou change
ebLeuecd notcating the peLclu ien
Acidl, ai bubblet 0ere_obAesed_
a eahanad tube dicaling he

Signature
Expt.No.. Page No.25

pgzluilienga bydemeiatien e_laclele

Lengnalfien detts
Colenit OTh Qheen_melallie Ahen

Letull

h pokumpiip est
Th iven te aniple_Conlaint
Calilinad
n The totCarlisunmk the peente
Cal"

Signature:
 SolaTien
Soalie Ctaphylococul auteu
CaphyoCocul auteuk
Expt. No. Date
Page No.98

Afm
lo olate the
The Qiven_ Aataphylatoctuk uMe
auHeu
Au ace plaie coale Sannple by
Coui dechniquue
KequicenMe+K
Telt ubl Dikikhdnal Sample
Cenical lalk atoclave
ga medlium
incubäldl hai ai
Poinple
and
TetalktaphylocoCuk QuLLUL Lalalian
enILmLLATen ueJul1eAl J
QLLalil eVelutfen in dilldnt
envitenme+ laphulachceul
abe o enhcehallueih
o tel ueuum ad o deiedd_lacibiak
LenhinRtbiun chldide ak a
fobibiti dol CØnlaminama
micuelda:

uoduceU
d AApelically Tuang leuLd_t Sample
kulpenkitn oto 3palaiplatut_abich
Signature :
 Date
Expt.No. Page No.99

Cenloin 5 n bioud paskex agat

Spliend 1nOClunuOveikugace
Agaqplote petciclchetAtesk beil
alalk AHoieaki plared_the
pdtouialxbet in pouiahi pe{Ttibn n
Lunl inOCulum
mCubalien_ 1hol
ebkeAved by aGa_and aHe
adinubal
inveL the a
plale
g6-312laka The
5-dp2
he 3 plal_
aVLLage

MelitM_poppaMlian ina edlenk

all_be _aboe
dsilld ill heal_oi
Inine
aditatt en_am heil
CeAplet dRoluve the edium
15 lhs_
Aitnclae
tempeadue_

CORing Kupplimeril
telluscte
Qbiualien AunaOuDded by lea
Black-Ahins-
developedd
luntnaly
bavp_bii ullyn
Zmek
Cpaaue Celenis
platek
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 Date
Expt. N o . . Page No.3 n

CenkikierC tOhen foucheo

Lekult
he Qiven Sample COiain
aphulochccul

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 olotion el Salmonella typhi Date
Expt.No. Page No. 3

AEm
isclate ddecled Salmenella
upbi the qiven anuple
tauipmeriks
Ado clave
Tet tube
Patoiplat
banis ube
Incubala
LAF Clanaina Cio lo
_ tle a Oven
Mat oialk:
EMB_ medium[£osin metbiline blug
mediumJatlap_hkyplele_beolh)

Painaple
falmenella aue spHLad
Deultou tn humanssuch al eakand
mepdKalmene lla kpecies Caukel an
inTe4irnal inlelfan în hunnank kngun
SalmenblleAik

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 Date
Expt.No.
Page No.22

eaceduse
Lepane Sample_hy puopeL
miLi maLke the dfutfond a
sheum_ n expaimeiL ToDDCulalel
each bee ubel _ST baajh
(Cenlaimina invaled bamtubel)
h 1 m Aanaple
Laes Oul the ame_opeIAl*on
ehe ailtion Ue LIOuA_ e l h
steile pipelit Pach iluifon
Tncubale lhi LST tube_a 35c J
Ag_hak keceidthe u b l heoing+
penuEfen Stueak Lplhk£MB 1LenD
dach_pe{tive tule in a c n
iAuele colenits
Otain IoCubale inveiledplalet
3n 24_h Lamin tbe plale
upial cel omie uie+l 4
each EMB plalee
PICk LeM
the niddle to hi
fouchin19
a d t a n A JeH_do a
Centue plan-
SanlLocubale th Aak
PCA Hempeualued 1g-hu
35

Signature-
 Date
Page N o . 3 3
Expt.No.

aank the qupunb PCA

Alanti
binlogical IMvT) -Jest
PeolnL theLenoydnn Aain in_a Amean1
18ba PCAAlanl
2LLpaLed

Signature: