TROPICAL MARINE BIOTECHNOLOGY Document No.

: 03
LABORATORY
WORK INSTRUCTON Effective Date :
MICROSCOPE Review Date :

Prepare by : Review by :

Salwa Layalya, A.Md.Si Prof. Dr. Ir. Agus Sabdono, M.Sc

Laboratory Technician Laboratory Coordinator
Date : Date :

Work Instruction

1.Connect the cable power to a power supply. Make sure the power supply is safe and
the cable powet is not twisted.
2.Turn on the light source, and rotate the light intensity knob to maximum intensity.
3.Adjust the width of the eyepieces so that you can comfortably see only one circle of
light.
4.Place the slide correctly on the stage. Pull back the slide clip to fit the slide into
position.
5.Use the stage adjustment knobs to position the sample over the condenser lens
6.Use the coarse focus to raise the stage as close as you can to the objective lens
7.Look through the eyepieces. You should be able to see a blurry image. Use the coarse
focus knob to slightly lower the stage until the material comes into focus. Note: if
examining material that is not visible on the slide, focus on the edge of the coverslip
first, then scan around for your sample
8.Adjust the iris diaphragm to see the effect this has on contrast.
9.After examining the leaf at scanning power, swing the revolving nosepiece so the low
power objective lens (yellow band) clicks into place. Use the fine focus knob to
improve the focus.
10. Slowly rotate the revolving nosepiece until the high power objective lens (blue band)
clicks into place. If you were in focus at low power, the lens WILL click into place
without contacting the slide. Use the fine focus knob to improve focus and the iris
diaphragm to improve contrast.
11. Use the stage adjustment knobs to position the leaf over the condenser lens.
12. Use the coarse focus to raise the stage as close as you can to the objective lens.
13. Look through the eyepieces. You should be able to see a blurry image. Use the
coarse focus