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Sivajith P R - LAST MINUTE HISTOLOGY - Quick Review of Histology and Cell Biology For Medical and Nursing Students (1st Edition) - Wolfrum (2021)
Sivajith P R - LAST MINUTE HISTOLOGY - Quick Review of Histology and Cell Biology For Medical and Nursing Students (1st Edition) - Wolfrum (2021)
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LAST MINUTE HISTOLOGY
Sivajith P R
Index
1) Muscle tissues
2) Salivary glands
3) Histology of stomach
4) Small intestine
5) Esophagus
6) Large intestine
7) Appendix
8) Liver & Gallbladder
9) Pancreas
10) Lymphoid tissue
11) Tongue
12) Skin
13) Cartilage
14) Trachea
15) Lung
16) Urinary system
17) Adrenal gland
18) Thyroid gland
19) Pituitary gland
20) Brain and spinal cord
21) Eye
22) Mammary gland
25) Ovary
26) Uterus
27) Fallopian tube
28) Male reproductive system
29) Embryological slides
30) Vascular tissue
31) Bone
32) How to draw
MUSCLE TISSUE
● Muscular tissue is responsible for movement of various parts of the body.
● Comprises of elongated cells called fibres.
● They are of 3 types: Skeletal, Smooth & Cardiac
◆◆◆
Smooth Muscle
-Non striated
INTERCALATED DISC :
Intercalated disc consist of 3 cell junctions :
● Desmosomes
● Gap junctions
● Tight junctions.
◆◆◆
H&E slide low magnification cardiac muscle
Skeletal Muscle
(eg: Muscles of limb & trunk)
Bands:-
The light and dark band(striations)are due to the presence of "A" and "I"
bands in myofibrils.Myofibrils are longitudinally running fibrils in the sarcoplasm
of skeletal muscle fibres(cells).
> I- band is known as dark band.The I band is bisected by a dark line called
Z-line ,& the segment between two Z lines is called a 'sarcomere',it is the
contracting unit of a muscle.
◆◆◆
H&E SLIDE : SKELETAL MUSCLE
SALIVARY GLANDS
SECRETION : Saliva.
• PAROTID
• SUBMANDIBULAR
• SUBLINGUAL
◆◆◆
FEATURES OF SALIVARY GLAND
°It possesses a connective tissue capsule
°This connective tissue capsule send septa, which divides the glandular
parenchyma into lobes and lobules
• SEROUS ACINI
Small with narrow lumen
•MUCOUS ACINI
•Large with wide lumen.
•SEROMUCOUS ACINI
Contain both Serous and Mucous cells. Sometimes serous cells form
"crescentic cap" on mucous acini called "SerousDemilunes".
SALIVARY DUCT SYSTEM
°Acini opens into the Intercalated duct.
°Intercalated ducts unite to form Striated ducts , both Intercalated and Striated
ducts are "Interlobular".
*Striated ducts are Striated because they have basal infoldings of plasma
membrane and longitudinal orientation of mitochondria between these infoldings
which gives the striated appearance.
◆◆◆
H&E slides
Lamina propria contain a large number of tubular glands, which open into
gastric pits.
These glands are oriented perpendicular to the surface.
MUCOSA
Lined by simple columnar epithelium. Mucous of pylorus shows gastric pits
which are deeper and occupy 2/3 rd of thickness of mucosa.
LAMINA PROPRIA
Filled with glands called pyloric glands, which consists of mucous secreting
cells and few parietal cells.
SUBMUCOSA
Composed of loose connective tissues.
MUSCULARIS EXTERNA
Same as that of fundus
Pylorus of stomach H&E low magnification
SMALL INTESTINE
Small intestine is divided into three:
1.duodenum
2.jejunum
3.ileum
DUODENUM
*It has four layers:
a.mucosa
b.submucosa
c.muscularis
d.serosa
*Muscularis and serosa/adventitia layers are the same as that of the general
structure of the alimentary canal. The duodenal mucosa is lined by simple
columnar cells (also known as "enterocytes") and goblet cells
*Each villi has a core of lamina propria with a central lacteal and blood
vessels.
3)Paneth cells-they lie in the deeper part of crypt they contain eosinophilic
secreting granules,their exact function is unknown
4)enteroendocrine(enterochromaffin cells)-secrete substances like
histamine.They possess granules which are stained by silver salt.
♦blood vessels
◆◆◆
H&E slide low magnification duodenum
JEJUNUM
>Jejunal villi are longer and more irregular(characteristic feature)
◆◆◆
H&E slide low magnification jejunum
ILEUM
• Here villi are more leaf-like.
• They contain germinal centers and are an important part of the lymphatic
system.
◆◆◆
H&E slide low magnification ileum
Oesophagus
Has four layers:
MUCOSA
Lined by non keratinized stratified columnar epithelium.
Muscularis mucosa has outer longitudinal and inner circular smooth muscle
layers.
SUBMUCOSA
Contains blood vessels, nerves, lymphatics and mucus secreting oesophageal
glands.
MUSCULARIS EXTERNA
Upper 1/3 is composed of skeletal muscles.
serosa/adventitia
composed of fibrous tissue
◆◆◆
H&E SLIDE : ESOPHAGUS
LARGE INTESTINE [ COLON ]
✳ Mucosa is lined by simple columnar cells with microvilli and goblet cells.
✳ There is no villi.
Mucosa
Submucosa
Muscularis
Serosa
❖MUCOSA
❃devoid of villi
❖SUBMUCOSA
❖MUSCULARIS EXTERNA
◆◆◆
H&E slide low magnification appendix
LIVER & GALLBLADDER
LIVER
• Liver is completely invested (covered) by a fibrous capsule called glisson's
capsule that lies deep to the peritoneal covering of the liver.
• This glisson's capsule is thickened at porta hepatis and sends trabeculae into
the interior of the liver and divides the liver parenchyma into incomplete
lobules.
These trabeculae which carry branches of hepatic artery, portal vein, hepatic
duct and lymphatics is known as portal tract or portalcanal.
Liver lobules are the structural unit of the liver.They are hexagonal in shape.
(iii) sinusoids - Irregular spaces b/w hepatic plates are occupied by liver
sinusoids.Which Are lined by discontinuous fenestrated endothelial cells.
Some endothelial cells that line sinusoids are modified to become phagocytic
kupffer cells.Sinusoids are filled with mixed arterial and venous blood from
hepatic artery and portal vein.
portal triad
◆◆◆
H&E SLIDE LIVER
GALLBLADDER
Gallbladder is a muscular sac It consists of 3 layers
Mucosa
Lined by simple tall columnar epithelium.
Fibromuscular layer
is made up of connective tissues and smooth muscle fibres.
serosa/adventitia;
consist of fibrous tissue
Some of the acini exhibits pale staining cuboidal 'centroacinar cells' within the
lumen.
They represent the 'intra-acinar' part of the intercalated duct.
◆◆◆
H&E slide low magnification pancreas
Lymphoid tissue
Lymph node
➡ lymph node possess a fibrous tissue capsule
◆◆◆
Spleen
➡ possess a fibrous capsule
White pulp
◆◆◆
H&E low magnification slide spleen
Tonsil
➡ tonsillar crypts are present
➡lymphoid follicles with the germinal centres are seen along the crypts
◆◆◆
THYMUS
1. Filiform papillae
2. fungiform papillae
3. circumvallate papillae
4. foliate papillae
⚠Epidermis
⚠Dermis
⚠Subcutaneous layer(hypodermis)
● Stratum basale
This is the deepest layer consisting of cuboidal or columnar cells. They show
mitotic activity. They undergo mitosis and give rise to new melanocytes
● Stratum spinosum
● Stratum granulosum
made up of three to five layers of flattened, fusiform cells. They are filled
with keratohyalin granules
● Stratum lucidum
● Stratum corneum
it is the most superficial layer consists of non- -nucleated, dead, scaly and
keratinized
◆◆◆
Thick skin
Thick epidermis (stratum corneum is thick)
Hair follicles and sebaceous glands are absent
◆◆◆
Thin skin
Thin epidermis (stratum corneum is thin)
Hair follicles and sebaceous glands are present
◆◆◆
● Chondrocytes (mature)
● Chondroblast (immature)
● Outer fibrous
● Inner cellular type
◆◆◆
HYALINE CARTILAGE
➡Characterized by "Translucent homogenous bluish matrix"
➡Here chondrocytes lie within the lacunae and they are arranged in groups of
2- 6 cells called "cell nest"
➡ Matrix around the cell nest we can observe lightly stained interterritorial
matrix
Note:
:) Chondrocytes are larger than those of hyaline cartilage and are present
"singly"or in small groups (groups of two or three)
:) Show elasticity
◆◆◆
TRACHEA
*TRACHEA has following parts:
MUCOSA
SUBMUCOSA
ADVENTIA
1.MUCOSA
4.ADVENTIA
Simple columnar
Respiratory bronchioles
Cuboidal epithelium
Alveoli
Squamous epithelium
H&E slide low magnification lung
Urinary system
contents................
1.kidney
2.ureter
3.urinary bladder
Kidney
➡ kidney consists of outer cortex and inner medulla
Cortex
➡ consist of
* Renal corpuscles
Medulla
➡ consist of
◆◆◆
kidney
Ureter
Mucosa
Muscular coat
◆◆◆
H&E SLIDE LOW MAGNIFICATION
Urinary bladder
Mucosa
Muscle coat
◆◆◆
H&E SLIDE LOW MAGNIFICATION URINARY BLADDER
Adrenal gland
Adrenal cortex
Consist of 3 zones
Adrenal medulla
consists of chromaffin cells which are arranged in between sinusoids
3)posterior lobe
Anterior lobe
Consist of three types of cells
Posterior lobe
Consist of large number of nerve fibers
Intermediate lobe
Contains large number of pale cells which usually surround follicles, filled
with colloids
H&E slide low magnification pituitary
Brain and spinal cord
Cerebellum
☢Consist of folia with outer cortex and inner medulla
Ganglionic layer consists of large pyramidal cells and few stellate cells
Polymorphous layer:consists of nerve fibres, stellate cells and the cells of
martinotti
cerebrum
Spinal cord
➡ consist of Central"H "shaped grey matter with the large anterior horns
and narrow posterior horns
◆◆◆
Eye
Cornea
Consist of
Corneal epithelium;
stratified squamous non- keratinized epithelium
Bowman's membrane;
it is the basement membrane of corneal epithelium
Substantia propria;
composed of parallel bundles of collagen and corneal cells
Descemet's membrane;
also known as posterior limiting membrane
Posterior epithelium;
composed of endothelium
◆◆◆
H&E slide low magnification cornea
Retina
☢Consist of 10 layers
pigment epithelium
outer plexiformlayer
inner nuclearlayer
inner plexiform layer
◆◆◆
Ovary
Covering of ovary outer germinal epithelium and inner tunica albuginea
Parts of ovary parts of ovary are outer cortex and inner medulla
➡ cortex shows ovarian follicles in different stages of development
Endometrium
Muscle layer
Serosa-mesothelium
◆◆◆
Male reproductive system
Prostate
histologically prostate consists of parenchyma and fibromuscular stroma
☢Glandular parenchyma
☢Stroma
◆◆◆
H&E LOW MAGNIFICATION SLIDE PROSTATE
Vas deferens
Consist of
☢MUCOSA
☢Muscle coat
thick, large, muscle coat consists of inner longitudinal, middle circular &
outer longitudinal smooth muscles
◆◆◆
Testis
➡Characterized by seminiferous tubules, lined with:-
1)spermatogonia
3)sertoli cells
◆◆◆
Epididymis
➡ epididymis is a comma shaped structure on the posterior- lateral aspect
of testis
◆◆◆
Embryological slides
Placenta
Maternal part-decidua basalis
◆◆◆
placenta
Umbilical cord
➡umbilical cord is covered by amnion membrane
◆◆◆
umbilicalcord
Vascular tissue
Basic theory
Elastic fibers
Bone
● The structural unit of a compact bone is osteon or haversian system
● Osteons are made up of concentric lamellae of bone matrix with a central
canal called haversian canal
● Within the bone matrix there are spaces called lacunae, which are
occupied by osteocytes
● Between the Osteons we can observe remnants of previous concentric
lamellae called Interstitial lamellae
● Each osteons are transversely connected by volkmann’s canal
How to draw
(HAND DRAWN DIAGRAMS)
BONE
THIN SKIN
thick skin
GALLBLADDER
PANCREAS
Thymus
LYMPH NODE
SPLEEN
Tonsil
ESOPHAGUS
DUODENUM
JEJUNUM
ILEUM
LIVER
APPENDIX
TONGUE
HYALINE CARTILAGE
ELASTIC CARTILAGE
FIBROCARTILAGE
TRACHEA
PITUITARY
ADRENAL GLAND
Thyroid gland
MAMMARY GLAND (INACTIVE)
MAMMARY GLAND (ACTIVE)
CEREBELLUM
CEREBRUM
SPINALCORD
Kidney
Ureter
Urinary bladder
Cornea
Retina
Testis
Epididymis
Vas deferens
Uterus proliferative
Uterus secretory
Prostate
Ovary
Placenta
UMBILICAL CORD
LARGE/ELASTIC ARTERY
MEDIUM SIZED ARTERY
LARGE SIZED VEIN
MEDIUM SIZED VEIN
SKELETAL MUSCLE
CARDIAC MUSCLE
FALLOPIAN TUBE
Smooth Muscle
Happy learning