Printed on: Tue Aug 03 2021, 07:18:01 AM Official Status: Currently Official on 03-Aug-2021 DocId: 1_GUID-ECB171D4-3F76-45CA-A6DE-7B4F5754F4FD_1_en-US

(EST)
Printed by: Le Tran Official Date: Official Prior to 2013 Document Type: USP @2021 USPC
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Add 2 drops of sodium nitrite solution (1 in 150) and 5 mL of

Tyrothricin
» Tyrothricin is an antibacterial substance
produced by the growth of Bacillus brevis Dubos
(Fam. Bacteriaceae). It consists principally of
gramicidin and tyrocidine, the tyrocidine usually
being present as the hydrochloride. It contains not
less than 900 µg and not more than 1400 µg of
tyrothricin per mg.
Packaging and storage—Preserve in tight containers.
USP Reference standards á11ñ—
USP Gramicidin RS
Identification—Add about 5 mg to 5 mL of
p-dimethylaminobenzaldehyde TS, and shake for 2 minutes.
water, and mix: a blue color is produced.
Loss on drying á731ñ—Dry about 100 mg, accurately
weighed, in a capillary-stoppered bottle in vacuum at a
pressure not exceeding 5 mm of mercury at 60° for 3 hours:
it loses not more than 5.0% of its weight.
Assay—Proceed as directed for gramicidin under Antibiotics—
Microbial Assays á81ñ, using a suitable, accurately weighed
portion of Tyrothricin dissolved quantitatively in alcohol to
yield a stock solution of convenient concentration. Dilute an
accurately measured volume of this solution quantitatively and
stepwise with alcohol to obtain a Test Dilution having a
concentration of tyrothricin assumed to be 5.0 times the
median dose level of the gramicidin Standard. Multiply the
result of the gramicidin assay by 5.0 to obtain the amount of
tyrothricin, in µg, in each mL of the Test Dilution.

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