Unit 4: Cell Structure

4.1: Studying Cells

 Cell: the smallest unit of a living things

Microscopy

- Cells vary in size and cannot be seen individually with the naked eye.
- Scientists use microscopes to study them, which is an instrument that magnifies an
object.
- There are two main types of microscopes used: light microscopes and electron
microscopes.

 Light microscopes: visible light passes and is bent through the lens system to enable the
user to see the specimen
- They are advantageous for viewing living organisms, but since individual cells are usually
transparent, special stains are used for easier viewing, which usually kills them.
- They can magnify up to approximately 400 times.

- There are two important parameters in microscopy: magnification and resolving power.

 Magnification: the process of enlarging an object in appearance

 Resolving power: the ability of a microscope to distinguish two adjacent structures as
separate: the higher the resolution, the better the clarity of the image

 Electron microscopes: use a beam of electrons instead of a beam of light

- Typically used to gain a better understanding of cellular structures and function.
- Allows for higher magnification and more detail and provides higher resolving power.
- Living cells cannot be viewed through an electron microscope.

Cell Theory

- In the 1600s, Antony van Leeuwenhoek observed the movements of single-celled

organisms which he termed “animalcules”.
- Robert Hooke then observed cork tissue through a lens in 1665, and discovered bacteria
and protozoa.

 Unified cell theory: states that all living things are composed of one or more cells, the
cell is the basic unit of life, and new cells arise from existing cells
- Proposed by Matthias Schleiden and Theodor Schwann in the late 1830s.
4.2: Prokaryotic Cells

- There are three basic types of cells: archaea, bacteria, and eukaryotes.
- Both archaea and bacteria are classified as prokaryotes, while cells of animals, plants,
fungi, and protists are eukaryotes.

- Most prokaryotic cells are smaller, and have a lower surface-to-volume ratio than
eukaryotic cells.

- In prokaryotic cells, DNA is organized into a single circular chromosome.

Components of Prokaryotic Cells

- All cells share 4 common components:

1. A plasma membrane 3. DNA
2. Cytoplasm 4. Ribosomes

 Prokaryote: a simple, single-celled organism that lacks a nucleus, or any other

membrane-bound organelles

 Nucleoid: the central part of the cell where the prokaryotic DNA is found

- Most prokaryotes have a peptidoglycan cell wall and many have a polysaccharide
capsule.
 Cell wall: acts as an extra layer of protection, helps the cell maintain its shape, and
prevents dehydration
 Capsule: enables the cell to attach to surfaces in its environment

- All prokaryotes contain chromosomal DNA localized in the nucleoid, ribosomes, a cell
membrane, and a cell wall.

- Some prokaryotes have flagella, pili or fimbriae.

 Flagella: used for locomotion (transportation)
 Pili: used to exchange genetic material during conjugation (reproduction)
 Fimbriae: used by bacteria to attach a host cell

Cell Size

- Prokaryotic cells are significantly smaller than eukaryotic cells, which have diameters
ranging from 10 to 100 μm.

- The small size of prokaryotes allowed them to quickly diffuse to other parts of the cell.

- In prokaryotic cells, any waste produced can quickly diffuse out.

 - Small size is necessary for all cells.
- Not all cells are spherical in shape, but they tend to approximate a sphere.
- If the cell grows too large, the plasma membrane will not have sufficient surface area to
support the rate of diffusion required for the increased volume. (As a cell grows, it
becomes less efficient.)

- Cell division, increasing surface area, becoming flat and elongated, or developing
organelles that perform specific tasks are ways for cells to become more efficient.
 These adaptations lead to the development of eukaryotic cells.

- The size of a cell is very important for its survival because as nutrients diffuse into the
cell, a sphere is the shape where nutrients would have to travel the least to reach the
center.
- This is important because nutrients and wastes are always exchanged at the periphery
of the cell. The shorter the distance they have to travel, the faster the exchange of
molecules.

4.3: Eukaryotic Cells

- All eukaryotic cells have:

1. A membrane bound nucleus
2. Numerous membrane-bound organelles (endoplasmic reticulum, Golgi apparatus,
chloroplasts, mitochondria, etc)
3. Rod-shaped chromosomes

- Organelles have specialized cellular functions.

The Plasma Membrane

- Eukaryotic cells have a plasma membrane, which is a phospholipid bilayer with

embedded proteins that separates the internal contents of the cell from its surrounding
environments.

- Controls the passage of organic molecules, ions, water, and oxygen into and out of the
cell.
 Wastes also leave the cell through the plasma membrane.

- The plasma membrane that specializes in absorption are folded into fingerlike
projections, called microvilli, and are typically found lining the small intestine.

The Cytoplasm

 Cytoplasm: the entire region of a cell between the plasma membrane and the nuclear
envelope
- Made up of organelles suspended in the gel-like cytosol, the cytoskeleton, and various
chemicals.

- It has a semi-solid consistency which comes from the proteins within it.
- Glucose, simple sugars, polysaccharides, amino acids, nucleic acids, fatty acids, and
derivatives of glycerol are found in the cytoplasm.
- Many elements dissolve in the cytoplasm and many metabolic reactions, such as protein
synthesis, also occur in the cytoplasm.
The Nucleus

 Nucleus: houses the cell’s DNA and directs the synthesis of ribosomes and proteins
- Is the most prominent organelle in a cell.
- Surrounded by two phospholipid layers

 Nuclear envelope: a double-membrane structure that constitutes the outermost portion

of the nucleus
- Both parts of the nuclear envelope are phospholipid bilayers.
- Is punctuated with pores that control the passage of ions, molecules and RNA through
the nucleoplasm and cytoplasm.

 Nucleoplasm: the semi-solid fluid inside the nucleus, where we find the chromatin and
nucleolus

 Chromosomes: structures within the nucleus that are made up of DNA

- In eukaryotes, chromosomes are linear structures. Every species has a specific number
of chromosomes in the nucleus of each cell.
- Are only visible from one another when the cell is getting ready to divide.
- When the cell is growing, chromosomes are attached to proteins, which create
chromatin.

 Chromatin: an unwound, jumbled bunch of threads; it describes the material that makes
up the chromosomes both when condensed and decondensed

 Nucleolus: a darkly staining area within the nucleus that aggregates the ribosomal RNA
with associated proteins to assemble the ribosomal subunits that are then transported
out through the pores in the nuclear envelope to the cytoplasm.

Ribosomes

 Ribosome: the cellular structure responsible for protein synthesis

- They appear as clusters (polyribosomes) or single, tiny dots that float freely in the
cytoplasm when seen through an electron microscope.
 - Ribosomes receive their orders for protein synthesis from the nucleus where DNA is
transcribed into messenger RNA.

- Are found practically in every cell, particularly abundant in cells that synthesize large
amounts of protein, because protein synthesis is an essential function of all cells.

Mitochondria

 Mitochondria: responsible for making adenosine triphosphate (ATP)

- Are oval shaped, double-membrane organelles (each membrane is a phospholipid
bilayer embedded with proteins)
- Have their own ribosomes and DNA

- ATP represents the short-term stored energy of the cell

 Cellular respiration: the process of making ATP using the chemical energy found in
glucose and other nutrients
- In the mitochondria, this process uses oxygen and produces carbon dioxide as a waste
product.

- Muscle cells have a very high concentration of mitochondria that produce ATP since
they need a lot of energy to keep the body moving.
Peroxisomes

 Peroxisomes: small, round organelles enclosed by single membranes

- Carry out oxidation reactions that break down fatty and amino acids and detoxify many
poisons that enter the body.
- Produce H2O2
- Get their name because hydrogen peroxide is produced during their oxidation reactions.

 Glyoxysomes: specialized peroxisomes in plants that are responsible for converting

stored fats into sugars

Vesicles and Vacuoles

- Membrane-bound sacs that function in storage and transport.

- Vesicles can fuse their membranes with other cells’ organelles or even the cell
membrane; vacuoles, however, cannot.
- Vesicles are usually smaller than the vacuole.
- The membrane of a vacuole does not fuse with the membranes of other cellular
components.

Animal Cells versus Plant Cells

- Both animal and plant cells have microtubule organizing centers (MTOCs).

- Animal cells have centrosomes, while most plant cells do not contain it.

- Plant cells have cell walls, chloroplasts and other specialized plastids, and a large central
vacuole.

Centrosomes

 Centrosome: a microtubule-organizing center found near the nuclei of animal cells

- Contains a pair of centrioles, two structures that lie perpendicular to each other
- Each centrosome is a cylinder of nine triplets of microtubules.

- It replicates itself before cell division, and the centrioles appear to have a role in pulling
the duplicated chromosomes to opposite ends of the dividing cell.

- The exact function of centrosomes is not clear since cells without it can still divide.
Lysosomes

 Lysosomes: contain enzymes that break down all 4 macromolecules

- Found in animal cells; not found in most plant cells
- Are active at a much lower pH that the cytoplasm; it is more acidic. This helps certain
reactions take place that cannot with the cytoplasm’s lower pH.

The Cell Wall

 Cell wall: a rigid covering that protects the cell, provides structural support, and gives
shape to the cell
- Made of cellulose and peptidoglycan (we cannot digest it)
- Fiber is essential to be consumed.

- Advantages of eating cellulose: pushes waste product and removes some of the fat of
the food you eat

Chloroplasts

 Chloroplasts: plant cell organelles that carry out photosynthesis

- Have their own DNA and ribosomes
- Have outer and inner membranes

 Photosynthesis: the series of reactions that use carbon dioxide, water, and light energy
to make glucose and oxygen
- Plants (autotrophs) are able to make their own food whilst animals (heterotrophs) must
ingest their food.

- Plants produce and consume CO2 to grow and develop.

- Contain a green pigment called chlorophyll, which captures light energy that drives the
reactions of photosynthesis.
- Photosynthetic protists also have chloroplasts.
The Central Vacuole

- Plays a key role in regulating the cell’s concentration of water in changing

environmental conditions.
- Supports the expansion of the cell
 When it holds more water, the cell gets larger without having to invest a lot of energy in
synthesizing a new cytoplasm.

4.4: The Endomembrane System and Proteins

The Endoplasmic Reticulum

 Endomembrane system: a group of membranes and organelles in eukaryotic cells that

works together to modify, package, and transport lipids and proteins
- Includes the nuclear envelope, lysosomes, vesicles, the endoplasmic reticulum, and the
Golgi apparatus.
- The plasma membrane is included within the endomembrane system because it
interacts with other endomembranous organelles.
- It does not include mitochondria or chloroplasts.

- Is a phospholipid bilayer, that can bulge out and create a sphere called a vesicle.

 Endoplasmic reticulum (ER): a series of interconnected membranous sacs and tubules

that collectively modifies proteins and synthesizes lipids
- These functions are performed in the rough and the smooth endoplasmic reticulum.

- The hollow portion of the ER tubules is called the lumen or cisternal space.
- Its membrane is continuous with the nuclear envelope.

Rough ER

 Rough endoplasmic reticulum (RER): the ribosomes attached to its cytoplasmic surface
give it a studded appearance when viewed through and electron microscope

- In the RER, newly synthesized proteins are transferred into the lumen where they
undergo structural modifications, such as folding or the acquisition of side chains.
- The modified proteins are incorporated into cellular membranes or secreted from the
cell.

- The RER makes phospholipids for cellular membranes.

- The phospholipids that are not destined to stay inside the RER will reach their
destinations via transport vesicles that bud from the RER’s membrane.

- RER is abundant in cells that secrete proteins since it engages in modifying proteins.
Smooth ER

 Smooth endoplasmic reticulum (SER): continuous with the RER but has few or no
ribosomes on its cytoplasmic surface

- Functions include: synthesis of carbohydrates, lipids, and steroid hormones;

detoxification of medications and poisons; and storage of calcium ions.

- In muscle cells, the sarcoplasmic reticulum (a specialized SER) is responsible for the
storage of the calcium ions needed to trigger coordinated contractions of muscle cells.

- The body doesn’t understand the difference between a medicine and a poison; all that
matters is the dose you take.

Cardiologist

- Polyunsaturated fats are better for the body than monounsaturated fats because they
lower the low-density lipoprotein (LDL) in the body and increase the high-density
lipoprotein (HDL).

- Saturated fat: doesn’t have a double carbon bond.

- Trans fats decrease the levels of HDL and increase the levels of LDL.
- We should be consuming this the least.

- The walls of the heart is composed of cardiac muscle tissue.

- We need calcium for muscles to contract. If the cells of a heart don’t work properly,
calcium levels decrease.
- The heart can also fail by the accumulation of low-density lipoprotein, which forms a
hard substance that restricts the movement around the heart.

Golgi Apparatus

 Golgi apparatus: a series of flattened membranes that sort, tag, and distribute lipids and
proteins
- Its receiving side is called the cis face, and the opposite side is called the trans face.
- Transport vesicles travel to the cis face, fuse with it, and empty their contents into the
lumen of the Golgi apparatus.
 As they go through it, they undergo modifications that allow them to be sorted.
- They are then tagged and routed to their proper destinations.

- Some proteins deposit their contents into other parts of the cell where they will be
used, and other fuse with the plasma membrane and release their contents outside the
cell.
 - Responsible of finishing the process of formation
- Short chains of sugar molecules: tag added

- The Golgi apparatus produces polysaccharides in plants, which are then incorporated
into the cell wall or used in other parts of the cell.

Lysosomes

 Lysosomes: the digestive component and organelle-recycling facility of animal cells that
is also a part of the endomembrane system
- It can use their hydrolytic enzymes to destroy pathogens that might enter the cell.

- Small vesicle that contains different types of enzymes.

4.5: Cytoskeleton

- There are many different types of filaments.

 Cytoskeleton: network of protein fibers that help maintain the shape of the cell, secure
organelles in a specific position, allow cytoplasm and vesicles to move within the cell,
and enable cells within multicellular organisms to move

- There are three types of fibers within the cytoskeleton:

1. Microfilaments
2. Intermediate filaments
3. Microtubules

Microfilaments

- Are the narrowest and function in cellular movement

- Microfilaments make the cell more rigid.
- Made of an actin subunit protein: tubulin. (the same protein as the ones in muscles)
- For actin to move, contract, form, and reform, it needs ATP.
- It enables it to engage in cellular events that require motion.

- The motor protein is made of myosin (thing that walks).

 - Myosin and actin: makes up muscles.

- Microfilaments provide some rigidity and shape to the cell and can depolymerize and
reform quickly.

HIV: zoonotic virus (mutated from animals to humans)

- Takes a long time to develop and attacks white blood cells, which are the ones that
protect the body from infections.
- As white blood cells were killed, people infected with HIV started to die from common
diseases.

Intermediate filaments

 Intermediate filaments: have no role in cell movement; their function is purely structural

- Made of several strands of fibrous proteins that are wound together

- Their diameter is between those of microfilaments and microtubules (8 to 10 nm)

- The most diverse group of cytoskeletal elements.

- Partly made up of keratin.

Microtubules

 Microtubules: small, hollow tubes

- Their walls are made of polymerized dimers of two globular proteins, a-tubulin and b-
tubulin
- Has a diameter of 25 nanometers (wider)
- Help the cell resist compression.
- *Video: what was being walked on
- Can disassemble and reform quickly, like microfilaments.

- Structural element of flagella (found in some bacterias and sperm cells), cilia, and
centrioles (the two perpendicular bodies of the centrosome).

Flagella and Cilia

 Flagella: long, hair-like structures that extend from the plasma membrane and are used
to move an entire cell
- Its structure is not the greatest.
- Not all cells have or need it.
  Cilia: extend along the entire surface of the plasma membrane; short, hair-like
structures that are used to move entire cells or substances along the outer surface of
the cell

- Both flagella and cilia share a common structural arrangement of microtubules called a
“9 + 2” array”.
- A single flagellum or cilium is made of a ring of nine microtubule doublets, surrounding a
single microtubule doublet in the center.

- Cilia can combine with other cells through sexual reproduction.

4.6: Connections between Cells and Cellular Activities

Extracellular Matrix of Animal Cells

- Red and white blood cells are inside our blood vessels and are part of our tissue.

- Integral proteins are critical for communication with the outside world.

 Collagen: the most abundant protein in the extracellular matrix of cells

- It keeps the cells together.

 Extracellular matrix: collagen fibers interwoven with proteoglycans (carbohydrate-

containing protein molecules)
- It holds the cell together to form tissue and allows the cells within the tissue to
communicate with each other.

- Glycoprotein: used for communication in the outside world

- Hormones: form of lipid that had a carbohydrate chain attached; connects with the cell
which causes growth, and the cells start dividing faster

- The primary components of the materials released into extracellular space are proteins,
most abundantly collagen.
 - There is communication between anything coming in or out of the tissue.

Intercellular Junctions

 Intercellular junctions: when cells communicate via direct contact

- Cells can do these two different ways:

 Plasmodesmata: numerous channels that pass between cell walls of adjacent plant cells,
connect their cytoplasm, and enable materials to be transported from cell to cell, and
throughout the plant

 Tight junctions: watertight seal between two adjacent animal cells

- Common in tissue.
- Each are made of proteins
- Their tight adherence prevents materials from leaking between the cells.
- Are typically found in epithelial tissues that line internal organs and cavities, and
comprise most of the skin.
- At the cellular level, organelles are held very tightly together.
Desmosomes

 Desmosomes: act like spot welds between adjacent epithelial cells

- Only found in animal cells
- Cadherins, short proteins, connect to intermediate filaments to create desmosomes.
- They pass through the membrane and prevent tissues to separate.

Gap Junctions

 Gap junctions: channels between adjacent cells that allow for the transport of ions,
nutrients, and other substances that enable cells to communicate
- It allows water and small molecules to pass between adjacent animal cells.
- Similar to plasmodesmata, that are located in plant cells, but differ structurally.
- They develop when a set of six proteins in the plasma membrane arrange themselves in
an elongated donut-like configuration, called a connexon.