- -,____

Bacteria
Selective medium
Staphylococci
Ludlam's
B.cereus
MYPA
B.anthrax
PLET
Legionella
BYCE
Borrelia
BSK
H.influenzae
Flide's
Pseudomonas
Cetrimide
Brucella
. ,
Serum potato infusion
Trypticase soy agar
Neisseria
Thayer-Martin
Mueller-Hinton
Clostridium
I Robertson cooked meat
Corynebacterium
Loeffler's serum slope
Tinsdale , cysteine tellurite blood agar
Campylobacter Skirrow's
Mycobacteria LI media
Shigella Deoxycholate citrate agar(DCA)
Nocardia Paraffin bait
Chlamydia Irradiated McCoy cells, He La cells
V parahemolyticus Wagatsuma agar

Bacteria Transport media

Str.pyogenes Pike's media
Gonococci Stuart's media
V.cholerae Cary-Blair, Venkatraman-Ramakrishnan
Shigella Sach's buffered glycerol saline

Culture appearance Organism

Golden yellow colonies Coagulase +ve staohvlococci
White colonies/oil paint Coagulase -ve sta.i;>hvlococ_ci
Matt, finely granular Virulent streptococci
Glossy colonies Avirulent streptococci ~
Spidery colonies/molar tooth/sunray Actinomyces
Pitting/corroding colonies Eikenella corrodens
Jet black colonies Salmon~11::i
Mulberry like clusters Ehrlichia ~
Satellitism H.influenzae
H.ducreyi (""
School of fish/rail track
Fish in st earn/swarm of gnats V.cholerae
Cl.perfringens
Stormy fermentation
Thumb print/Aluminium paJDt/ Bordetella_
-
Bisected oearl ; /mercurv rlmos
Yersinia pestis
Stalactite growth
Mycoplasma
Fried egg colonies
Pneumococci
Draughtsman/carom coin

Type of motility
Bacteria
Campylobacter, V cholerae Darting
Tumbling
Listeria (at 20-25QC)
Gl iding
Mycoplasma
Stately
Clostridia
Lashing
Borrelia
Twitching
Trichomonas
Cork screw
T pallidum
Spinning motility
Fusobacterium
Test/ Reaction Organism -7
-._J

--
I

Dick test, Schultz Chartlon test Scarlet feve r I

I
Shick test Diphtheria i
M'Fadyean's reaction Bacillus anthrax I
' I II
Ascoli's thermoprecipitin test I

Diazo reaction Salmonella typhi i

-j
Diene's phenomenon Proteus I
7
String test Vibrio i
7
Nagler reaction Cl .perfringens :
Quelling reaction
Frei's test
Pneumococcus
LGV
JI
Eiken' s precipitin test ETEC
7
I

Organism Growth factors 7

E coli Arginine '
I
Gonococci Glutathione I
l
S typh i Tryptophan
I
Legionella L-cysteine
'
Mycoplasma Cholesterol
~
M tuberculosis Niacin
Rickettsia Sulfonamides
H influenzae Factors V and X
H ducreyi Factor X
H parainfluenzae Factor V '
I

V parahemolyticus NaCl
Rotavirus Trypsin I

Organism Other name

I
H.aegypticus Koch -Weeks' s bacillus I
i
H.i nfluenzae Pfeifer' s bacillus
Ps. pseudomallei Whitmore' s bacillus I

M .paratubercul osis Jone' s bacil lus i

M.intracellulare · Battey' s bacillus I
C.diphtheriae Kleb's Loeffl er's baci llus
C.pseudotuberculosis Prisch-Nocard bacillus !
1
C.pseudodiphtheriticum Hoff man s bacillus
I
Klebsiella pneumoniae Fnedlander's bacillus
Klebsiella rhinoscleromatis Frisch bacHlus
'
Klebsiella ozaenae " Perez or Abel' s bacillus i
'
Legionella micdadel Pittsburg pneumonia age nt - I
Mycopl~sma pneumoniae Eaton agent
-c..te.ta_ni. ,_ - Nicolair' s baciHus

-~~ -:
,_ Organism I
'
/ ~dlstal1iactylitis
, r,;_'- • -·,
Staphylococcus aureus or
I
I

. ,· ~~- .'
,,
Strept ococcus pyogenes '
I

_:~ -impetigo/Ecthyma/Furunculosis Staphylococcus au reus

: E~las· ·· Strepto.coccus pyogenes '
Impetigo contagiosa
-Necrc,tizia,g -fasciitis Streptococcus pyog-enes or I

Clostridium £pecles
_Ecthyma gangrenosum Pseudomonas,aeruginosa '
"
Hot tub folliculitis
Erythrasma Corynebacterium minut:issimum I

Erythema migrans :
-l BorrelJ~ burgdoferi I

Fish tank or Scwimming pool gran41oma Mycobacterium ;marinum

8uruli ulcer ..
-Mvcab~d:,num:u~rans ;

Verruca peruana ·,
~Qp--oirn~s
' ,~·._.-,'.~~•.~if1.td~1• ·J ... 1, .:--..1. i: · '"
 Fever
Shangai fever I Organism )

Undulant/Malta fever Pseudomonas

I
Brazilian purpuric fever l Brucella
Haverhill/Rat bite fever I H.aegypticus
Sodoku 5t reptobacillus moniliformis
· Cat scratch disease Spirillium minus
I
Oraya fever/ Carrion's ct· 1 Bartonella henselae
rsease
, Goal fever/ epidemic ty hu Bartonella bacilliformis
I T p s
Vi. e1 s disease R.prowazekii
Leptospira
Organism
Sta ph aureus Virulence factors
Streptococci Coagulase
Pneumococci M protein
Gonococci I Capsular polysaccharide
Pili
E coli
Pili
Cl perfringens
Lecithinase
rv1 tube rcu losis
Cord factor

Acid fast organisms I Intracellular organisms Capsulated bacteria

,
Mycobacteria
lsospora
I• M.leprae, M.tuberculosis
• Meningococci
• Legionella, Listeria
, Cryptococcus cyst • Pneumococci
• Rickettsiae, Chlamydiae
, Nocardia
• Cl perfringens, Cl butyricum
• Brucella, Bordetella
• H influenza
, Rhod ococcus • Pneumococci
• Klebsiella
Bacterial spores • Salmonella, Shigella • Ecoli
, Legionella micdadei • Meningococci, Gonococci • Yersinia
, Sperm head • Ypestis
• Bordatella
• Bacillus anthrax
• Vibrio parahemolyticus

Toxins inhibiting protein synthesis Gram negative organisms producing exotoxin

• Sh.dysentriae • E.coli, Salmonella, Shigella
Diphtheria • Vibrio
• Pseudomonas • Pseudornonas
, Verotoxin of E.coli • Bordatella pertussis

Gram negative rods Gram negative coccobacilli Microaerophilic bacteria

• Enterobacte riaceae • Hemophilus • Spirochetes
, Vibrio • Bordatella • Campylobacter
• Campylobacter • Brucella • H pylori
_ • ____:_~
H.pylori
c.:...:..:_ _ _ _ __ __ j _•_ _
Legionella
-'=---- - - -- -------j • M bovis
Gram negative cocco bacillary rod - Acinetobacter • Borrelia burgdoferi

Urease positive Oxidase positive Catalase negative

• Proteus • Haemophilus • Streptococci
• Klebsiella • Vibrio • Pneumococci
• S.aureus • Neisseria • EJ1terococci
I I H.pylori • Legionella • .Almost all other organisms are
catalase positive
• Nocardia • Pseudomonas .. ., '
• Yersinia • Brucella ...
• Cryptococcus I • Pasteur ella ,,
>I·' .;;

• Mycobacreria except MAC l .,

'f ' · :
.'.•:·.
..
 -:rl~crit:r ~~..;i~b·,--,:-o-n~-1m-.-p-ti_o_r,i_o_f_ra_ w_m_,-it-k-----r--ln-fe-,-t-io_n_t~h-:r-o-u-gh:--.7b-:-i,~d:-e~x--::c-re~t:--a- - -~ "-
• L.st~~a • Chlamydia psittaci
~n ...c'::c{fa • .( ryptOCO·CCUS

Car,~vtoc,acter Jej uru • H.istoplasma capsulatum

Babes-Ernst granules are seen in

• l\fyccp /asm a • Corynebacterium diphtheria
• i\'Tycobacterium t uberculosis • Bordetella pertussis

I
' . Stre ptooacillus moniliformis
Sta phyiococcus aureus (in the presence of penicillin)
• Gardenella vagina/is

Pfeomorphic bacteria Relative bradycardia (Faget's sign) Bipolar staining - safety pin appeara
,r -------:--'--:""--------- - ---t-.:..:..::.....:.......:....:.....:.::___::_..:._,!__ __.!,_~----=--~t-_;;._--:---:--------=------=---~~~~nce ,
! • C diphtheriae • Typhoid • H.ducreyi '
I I
i• H in flu enzae • Legionnaire's disease • Y. pestis I
· • Ricketts iae • Psittacosis • Pasteurella /
• Streptobacill us moniliformis • Yellow fever • Burkholderia pseudomallei i
I

C_a_m_p_•....;;g_ra_n_u__!_o_m_a_ti_s______l _,.
1
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