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10 Pro 1
10 Pro 1
Lambunao, Iloilo
SCIENCE TECHNOLOGY AND ENGINEERING
S.Y. 2021 - 2022
-
Lambunao, Iloilo
JM Laurence L. Lavilla
March 2022
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TABLE OF CONTENTS
CONTENT PAGE
Figures 5
Table 6
I. Introduction
Objectives 15
Hypotheses 16
Definition of Terms 17
Research Design 22
Procedure 23
4
Species Identification
Preparations of Treatments 24
Data Collection 24
Data Analysis 27
Budget Proposal 28
References 34
5
FIGURES PAGE
Figure 2. Image of the TDS Meter, TDS & EC Meter and pH Meter 24
TABLE
Chapter I: Introduction
This chapter shows the overview of the study. It contains the Background and Conceptual
Framework of the Study which gives some explanations on what our study is all about. Diagram
of the Conceptual Framework that shows the paradigm of the study which presents the
relationship between different variables in this study, Statement of the Problem and Objectives of
the Study that shows the study’s aims and all of the objectives set by the researchers for their
study, Significance of the study which presents the benefits and advantages of the study towards
others, explains why our study is significant. Definition of Terms which includes the important
words and their meanings in the study which explain the usage of the words that were used in the
study and the Scope and Delimitation of the study in which it elucidates the scopes and
In the Philippines, 91% of the country's estimated 100.7 million people have access to at
least basic water services; however, access is highly inequitable across the country, with regional
basic water service access ranging from 62% to 100%. Around 99 percent of the richest one-fifth
of households have access to basic water services, compared to only 80 percent of the poorest
Nearly five million people in the Philippines rely on unsustainable and dangerous water
sources (Water org., 2018). Approximately 9 million people do not have access to improved
sanitation. Despite its rising economy, the Philippines have serious problems with access to
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water and sanitation. Some rapidly expanding cities are also having difficulty providing
appropriate water and sanitation facilities to their additional citizens. The majority of
communities rely on natural bodies of water for water purification and daily consumption
(Ambulkar, n.d.)
According to new research by the World Health Organization and UNICEF (2017), 3 in
10 people, or 2.1 billion people, lack access to clean drinking water at home, and 6 in 10 people,
In urban Iloilo, there is very little wastewater treatment. Most hotels, hospitals, business
buildings, and government facilities, according to reports, process wastewater on-site. On the
other hand, most residential development relies on septic systems, which are said to be poorly
In Iloilo River, the coliform level is well above the standard, which is 200 MPN (most
probable number) per 100 milliliters, according to the latest laboratory test findings from the
Environmental Management Bureau (EMB) Region 6's Ambient Water Monitoring Unit (mL). It
has been persistently high since 2013, according to EMB's five-year Physico-chemical and
According to the data of Numbeo, the world’s largest database of user-contributed data
about cities and countries worldwide, pollution in Iloilo regarding water pollution is 81.25% and
the purity and cleanliness in Iloilo regarding water quality is 18.75%. The data was based on the
visitors of the site in the past 3 years. Numbeo was created last 2009. It is a crowd-sourced
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global database of quality of life data, such as housing indices, perceived crime rates, and
healthcare quality, among other things. It provides a tool for viewing, sharing, and comparing
information about the cost of living around the world, as well as a system for conducting
systematic research on the cost of living and property markets, as well as a system for
conducting other systematic economic research on a large dataset with data from around the
world. Numbeo relies on the collective wisdom of the population to gather the most accurate data
possible.
Lambunao stated that the uncleaned and untreated water in the Municipality of Lambunao is 132,
183 cu. m.
Toxic compounds enter water bodies such as lakes, rivers and seas, where they are
dissolved, suspended in the water, or deposited, resulting in water pollution. Water quality
number of solid grades such as block, kibbled or ground and is also available as a solution. The
efficacy of using Aluminum Sulfate for the treatment of a water sample from the cavouco stream
was studied in this study. The researchers also looked at the residual aluminum concentration in
the treated water. It also has an alum demand for hydroxide and alkalinity and only works within
a certain pH range. Aluminum exerts undeniable human health, its content in water treatment
plants should be limited. Even Aluminum Sulfate is fairly non-toxic. However, alum can still
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cause irritation, burns, and respiratory issues. If inhaled it may cause headaches, nausea, and
In this study, researchers will be cleaning the river water samples in Brgy. Legayada from
the municipality of Lambunao using Gabing Uwak (Monochoria vaginalis) with the process of
rhizofiltration. The researchers chose to use the river water as their dependent variable, Gabing
Uwak as independent variable, Aluminum Sulfate as positive control, and rhizofiltration method
River water can be used for a variety of purposes as a surface water source. River water
availability is highly dependent on the needs of the local system. It is particularly abundant in
riparian areas with a consistent flow throughout the year. Water extraction must be managed and
regulated properly to maintain the ecology and avoid user conflict. In underdeveloped countries,
an estimated 90% of all wastewater is dumped untreated into rivers, lakes, and seas. (Stauffer, et,
al. 2019). Even if the water in a stream, river, or lake appears to be clean, it may contain bacteria,
viruses, or parasites that can cause waterborne diseases like cryptosporidiosis or giardiasis. It is
The researchers will look for the plant heart-shaped false pickerelweed commonly called
Gabing Uwak. This plant can be found at low and medium altitudes. It is typically found in open
wet places, sunny sites in swamps, rice paddies, ponds, marshes, rice fields, and along ditches.
The plant roots in mud, with its upper parts growing above water. Its reproduction is mainly from
seeds. Gabing Uwak is a plant of subaquatic to aquatic conditions and is a native in Asia. In the
Philippines, the flowering of the plant can occur throughout the year. Due to the massive growth
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of Gabing Uwak, a type of weed, it tends to be killed. It is better to use them as a tool to clean
The researchers will use rhizofiltration method to purify the river water with Gabing
Uwak. Rhizofiltration, which involves using plants (both terrestrial and aquatic) to absorb,
concentrate, and precipitate contaminants from polluted aqueous sources with low contaminant
concentration in their roots, is used to deal with effluents discharged by industries, agricultural
run-off, and acid mine drainage (Mohd, et al., 2015. Rhizofiltration plants should have fast-
growing roots and the ability to remove pollutants from solutions for a long period (Dhanam,
2017).
This study relates to Sustainable Goals and Development (SDG), Goal 6: “Ensure
availability and sustainable management of water and sanitation for all”. Improve water quality
by reducing pollution, eliminating dumping and minimizing release of hazardous chemicals and
materials, having the proportion of untreated wastewater, and substantially increasing recycling
Clean Water and Sanitation for all, more than 80% percent of wastewater resulting from
human activities is discharged into rivers or sea without any pollution removal. Over 1.4 billion
people currently live in river basins where the use of water exceeds minimum recharged levels.
90% of the global population of 6.8 billion people used at least a basic service. Basic service is
an improved drinking water source within 30 minutes to collect water within a round trip.
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The research is also related to the Harmonized National Research and Development
Agenda (HNRDA), underWater Security, water quality, accessibility, and availability, which
includes the pollutants and contamination whether surface or groundwater, including analysis of
historical flows, sediment, and toxicity loads of lakes and rivers, weather modification for
Figure 1
Note: The conceptual framework shows the experimental group (Gabing Uwak)
with the control groups (Aluminum Sulfate as positive control and No treatment as
negative control) as treatment will be used to the water samples. The results on the
dissolved solids, and electrical conductivity) and chemical parameter (pH level).
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1. What are the physical parameters (temperature, total dissolved solids, and electrical
conductivity) of the river water samples after the treatment of: A.) Gabing Uwak plant, B.)
450 mg Aluminum Sulfate (positive control) and C.) No treatment (negative control)?
2. What is the chemical parameter (pH level) of the river water after the treatment of: A.) Gabing
Uwak plant, B.) 450 mg Aluminum Sulfate (positive control) and C.) No treatment (negative
control)?
3. Is there a significant difference in the physical parameters of the river water after the treatment
of: A.) 3 pieces of Gabing Uwak plant, B.) 450 mg Aluminum Sulfate (positive control) and
4. Is there a significant difference in the chemical parameter of the river water after the treatment
of: A.) 3 pieces of Gabing Uwak plant, B.) 450 mg Aluminum Sulfate (positive control) and
Objectives
This study aims to determine the effectiveness of Gabing Uwak (Monochoria vaginalis)
as Rhizofiltration for Purifying River Water. Specifically, it seeks to meet the following:
1. Examine the physical parameters (temperature, total dissolved solids, and electrical
conductivity) of the river water after the treatment of: A.) 3 pieces of Gabing Uwak plant, B.)
450 mg Aluminum Sulfate (positive control) and C.) No treatment (negative control).
2. Examine the chemical parameter (pH level) of the river water after the treatment of: A.) 3
pieces of Gabing Uwak plant, B.) 450 mg Aluminum Sulfate (positive control) and C.) No
3. Determine if there is a significant difference in the physical parameters of the river water after
the treatment of: A.) 3 pieces of Gabing Uwak plant, B.) 450 mg Aluminum Sulfate (positive
4. Determine if there is a significant difference in the chemical parameter of the river water after
the treatment of: A.) 3 pieces of Gabing Uwak plant, B.) 450 mg Aluminum Sulfate (positive
Hypotheses
1. There is no significant difference in the physical parameters of the river water samples using
2. There is no significant difference in the chemical parameter of the river water samples using
three treatments.
Community - This study can improve quality and produce clean water. Ensure safe water for
daily necessities.
DAI- This study helps the organization to improve and discover more sanitation techniques.
Household – This study could help them to learn more alternatives to provide clean water and
Students - In this study, the students could benefit with the basic concept of rhizofiltration that
they may acquire, they can also enhance their learnings and abilities in the said field.
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Future researchers - For those who would like to adopt the study regarding rhizofiltration as the
basis for them to innovate and create new versions they could use the study as a guide for them
to conduct.
Definition of Terms
The following terms are defined according to their function in this study:
Aluminum Sulfate (n.) – is a white or off-white crystal, powder that is used for water
In this study, aluminum sulfate is used as the positive control of the study because it is
Filtration (n.) – removes particle suspension and impurities in water, it eliminates risk and
In this study, filtration is the process that researchers will use to filter the particles
Gabing Uwak (n.) - the heart-shaped fake pickerelweed, also known as oval-leaved pondweed,
a blooming plant of the water hyacinth family. Its young leaves are
In this study, Gabing Uwak will be used as water treatment for river water samples.
pH Level (n.) - is a measure of how acidic/basic water is (Water Science School, 2019).
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In this study, pH level is a chemical parameter that will help the researcher to determine
the water quality of the river water samples and the effectiveness of the Gabing Uwak.
pH Meter (n.) - an electronic instrument used to determine the amount of hydrogen-ion activity
In this study, the pH meter will help the researcher to determine the effectiveness of
Gabing Uwak as a potential water purifier, also to read the comparison of the result of a sample
Post-Treatment (n.) - it is the treatment carried out after some earlier action or process has been
In this study, post-treatment refers to the final results of the water samples which will be
In this study, pre-treatment refers to the result of the water samples before conducting
River (n.) - a big natural stream of fresh water that flows along a defined route, generally
In this study, the river water will receive treatment from Gabing Uwak.
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Total Dissolved Solids (TDS) & Electrical Conductivity (EC) Meter (n.) - it allows you to test a
variety of water sources fast and accurately at home, school, the office, or
anywhere else you need to evaluate water quality (WQ1923, July 2019).
In this study, the Total Dissolved Solids (TDS) & Electrical Conductivity (EC) Meter is
one of the devices that shows how well certain materials can carry electricity. By determining the
level of EC, the researcher can also see how polluted water is.
Total Dissolved Solids (n.) - the number of dissolved compounds in the liquid as measured by
In this study, Total Dissolved Solids is one of the physical parameters that will be used to
determine the amount of dissolved particles in the water, usually in parts-per-million or ppm.
Rhizofiltration (n.) - is the adsorption or precipitation of dissolved substances onto plant roots, or
Our study will focus on rhizofiltration of river water using Gabing Uwak plant.
Aluminum Sulfate will be used as the positive control and river water without Gabing Uwak as
The 45 pieces of Gabing Uwak (Monochoria vaginalis) plant will be collected from Ms.
Sophia Lenaming’s residence at Brgy. Sibaguan, Lambunao, Iloilo. Meanwhile, the river water
samples between 7:00 - 8:00 in the morning at Brgy. Legayada, Lambunao, Iloilo.
Three set-ups of river water samples will be prepared with fifteen replicates each. Every
fifteen containers will contain 3 pieces of Gabing Uwak plant, 450 mg Aluminum Sulfate, and
The experimental period will last for 2 weeks. It will be conducted at Ma. Sophia
This study is only limited in determining the effect of Gabing Uwak (Monochoria
vaginalis) used as water treatment on the physical parameters (temperature, total dissolved
This study does not cover the area outside the process of determining the effectiveness of
The researchers will make sure that the environment where the setups will be located is
free from pollution and other factors in the environment that can affect the effectiveness of
plants. Under the guidance of Mr. Lacupa, the researchers will conduct rhizofiltration of river
water utilizing Gabing Uwak (Monochoria vaginalis) with the purpose of safeguarding the
Statistical tools that will be used in this study are Mean for determining the average of the
data collected from each setup and One-way ANOVA to compare the averages of the data from
This chapter includes the Research Design which shows the framework of the research
methods and techniques, the Procedure which presents the step–by–step process that the
researchers will perform to obtain the results, Risk and Safety which indicates the potential risks
to the researchers and the environment, and the safety precautions that can be done to prevent or
control them, Data Analysis which presents the statistical tools to be used to analyzed the results,
and Budget Proposal which shows the list of possible expenses and their estimated costs.
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Research Design
data that can be transformed into usable statistics. It is used to quantify attitudes, opinions,
behaviors, other defined variables, and general results from a larger sample population.
Quantitative research uses measurable data to formulate facts and uncover patterns in research.
(Dovetail, 2017). The researchers pursued this type of research because they wanted to
statistically find out the effectiveness of Gabing Uwak (Monochoria vaginalis), and the positive
more variables using a scientific approach. The first variable is a constant that you may change
to see how the second variable is affected (“Experimental Research”, n.d.). The researchers used
this kind of quantitative research because they wanted to find out if there's any difference in the
Procedure
For the researchers to identify the plant species in a different method, they will download
the app "Picture This" to identify the plant species to be used. The researchers will next use the
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app to photograph the Gabing Uwak plant in order to determine its scientific name. For double-
checking of the plant, it will also be verified by Mr. Renio Lacupa, a DENR employee, and Jeaza
The researchers will collect the Gabing Uwak (Monochoria vaginalis) at Brgy. Sibaguan,
Lambunao, Iloilo. They will use a shovel to gather the plants and place it in the bucket. The
plants will be carefully washed and placed in a safe area. The plant will be weighed by the
researchers using a digital weighing scale with the average weight of 365 - 380 grams.
The researchers will collect the river water samples between 7:00 - 8:00 in the morning at
Brgy. Legayada, Lambunao, Iloilo. They will use a bucket to gather the water samples in the
river. The river water samples will be put in a safe or secured place and will be covered by a
Preparation of Treatments
The study consists of three treatments namely: a.) 3 pieces of Gabing Uwak plant, b.) 450
mg Aluminum Sulfate (positive control), and c.) No treatment (negative control). Then,
researchers will prepare 45 containers for the three treatments. Next, river water samples will be
put on three treatments with fifteen replicates in an equal measurement of 3L. Lastly, the
researchers will observe the river water samples for two weeks after they added the three
treatments.
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Data Collection
River water samples will be distributed to 5-10 Liter containers namely; Treatment A.) 3
pieces of Gabing Uwak plant, B.) 450 mg Aluminum Sulfate (positive control) and C.) No
treatment (negative control). Treatment A will undergo rhizofiltration using 3 pieces of Gabing
Uwak plant while treatment B will receive 450 mg aluminum sulfate and treatment C will be set
aside.
In two weeks of observation, river water samples will be tested every week using the
devices such as pH meter for acidity and alkalinity, TDS and EC meter for total dissolved solids,
electrical conductivity, and temperature. Water quality parameters will be listed namely: 1.)
Physical Parameters: temperature, total dissolved solids, and electrical conductivity, and 2.)
Figure 2
Filtration
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The river water samples will undergo filtration before receiving the different treatments
using cotton linen fabric to remove the particles and impurities of the water that settled at the
bottom. Researchers will place the cotton linen fabric at the top of an empty big blue tub and
pour the river water samples through it. After that, it will be filtered for the second time from the
tub to the containers of where the treatments will be added later on. Filtration will remove solid
particles, it permits fluid to pass through but retains solid (“Filtration”, n.d.).
Temperature Test
The temperature of water samples would be determined every week by conducting the
treatments using the TDS & EC meter device to check whether it is safe for human consumption
and usage (for bathing, cleaning, and washing of things such as clothes and dishes). Then, the
difference in the water treatments every week testing will be recorded by the researchers. The
Total dissolved solids of water samples will be observed every week by conducting the
treatments using the TDS meter device to check whether the Gabing Uwak (Monochoria
vaginalis) is effective in purifying the river water samples. River water samples with the normal
level of total dissolved solids are 600 ppm - less than 300 ppm shows a good quality of water.
Figure 3
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Water samples will be tested using the TDS & EC meter device every week by
conducting the treatments to distinguish their electrical conductivity that could tell whether the
river water samples are clean or not. River water samples with 100 - 500 μS/cm shows a good
quality of water.
Figure 4
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pH Level Test
The water samples will be tested every week by conducting the treatments using the pH
test paper and pH meter device. The pH test paper will be half-dipped in the river water samples
to check if it is very acidic, very alkaline, or neutral by basing the color it shows on the pH level
chart after a few seconds. The pH meter will be slightly dipped in river water samples for 5
seconds with three different kinds of buffer solutions namely; pH 4.00, pH 6.86 (tap once), pH
9.18 (tap twice). The neutral pH is 7 which is indicated by the green color.
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Figure 5
There may be hidden debris or underwater hazards which can cause injury, including
weeds and plants which can entangle people under the water. If it's polluted it could make you
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ill. it can be very difficult to get out (especially steep slimy banks) there are no lifeguards on
To reduce the danger of injury from collecting Gabing Uwak (Monochoria vaginalis) and
river water, researchers must wash their hands and feet after gathering the materials needed for
the experiment. In addition, adequate safety in using equipment such as face masks to protect
To avoid another risk that the researchers may face with the use of chemicals like
aluminum sulfate which is dangerous, researchers will be extra careful and will use protective
If aluminum sulfate is combined with water, it forms sulfuric acid which causes skin and
eye irritation. It irritates the lungs and throat, produces coughing and shortness of breath when
inhaled, while creating red rash, itching, and burning if contact with the skin. Aluminum sulfate
causes a severe effect on the intestinal walls and stomach when consumed causing a person to
To avoid further dangers after conducting the treatments, the researchers will be
responsible for the tools used, especially the sharp ones, they will be cleaned and will be put in a
Data Analysis
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The researchers will use two statistical tools namely: Mean and One-Way ANOVA to
assess the data that will be gathered after the experimentation has concluded.
Mean
The Mean is the average you’re used to, where the numbers are all added up and then
In this study, Mean will be used to measure the average of the parameters of the river
water samples in the study namely: 1.) Physical parameters (temperature, total dissolved solids,
One-Way ANOVA
The One-Way Analysis of Variance (ANOVA) is used to determine whether there are
any statistical differences between the means of three or more independent groups (“One-Way
ANOVA”,2018).
In this study, One-Way ANOVA will be used to determine the average result of the
following three treatments: 1.) If there are significant differences in the physical parameters of
the river water samples among the three treatments, and 2.) If there are significant differences in
the chemical parameter of the river water samples among the three treatments.
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Table 1
Budget Proposal
Php)
pH Strips 2 166.00
1230.04
TOTAL
Note: The budget proposal for this study shows the list of items/materials and services
needed with their corresponding prices. The total cost of conducting this study is
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