Professional Documents
Culture Documents
1 3 Spectra
1 3 Spectra
1 3 Spectra
Workflow of MALDI-Tof MS
1
What can you import?
Uncompressed
grayscale TIFF
images
8, 12 or 16 bit
Peak tables
BioNumerics®
- Fingerprint data module
- Dedicated experiment
Densitometric for spectral data
curves from
automated
sequencers
MALDI, HPLC,
LC/MS,…
3
2
Import is made easy using default import templates
Raw curve
After baseline
subtraction and
smoothing
3
Easily summarize technical of biological replicates
Data matrix
(set of banding patterns)
Coefficient
Similarity matrix
Clustering
Dendrogram/grouping
4
Similarity can be calculated using peaks or full curve
…
…
… % similarity … % correlation
Takes into account
Only takes into account Exception: quantitative values of full
presence/absence of bands peak based curve
Pearson
correlation Pearson correlation, Cosine
Jaccard, Dice, Jeffrey’s x, Ochiai correlation
9
A B C D
B 68 100 1 1 1 0
C 77 96 100 1 1 1 1
D 97 85 72 100 1 0 0 1
Coefficient 0 1 1 0
1 0 0 1
Similarity matrix 1 0 1 1
10
1 1 1 1
5
A dendrogram helps detect relationships
11