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Biotechnology 3
Biotechnology 3
Biotechnology
Quarter 1 – Module 3:
Laboratory Apparatus and
Equipments in Biotechnology
Biotechnology – Grade 8
Alternative Delivery Mode
Quarter 1 – Module 3: Laboratory Apparatus and Equipment in Biotechnology
First Edition, 2020
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Management Team:
Schools Division Superintendent : Romeo M. Alip, PhD, CESO V
Asst. Schools Division Superintendent : Rolando M. Fronda, EdD, CESE
Chief Education Supervisor, CID : Milagros M. Peñaflor, PhD
Education Program Supervisor, LRMDS : Edgar E. Garcia, MITE
Education Program Supervisor, AP/ADM : Romeo M. Layug
Education Program Supervisor, Science : Edwin R. Bermillo
District Supervisor, Limay : Elma P. Dizon
Division Lead Book Designer : Kenneth G. Doctolero
District LRMDS Coordinator, Limay :
School LRMDS Coordinator :
School Principal :
District Lead Layout Artist, Biotechnology :
District Lead Illustrator, Biotechnology :
District Lead Evaluator, Biotechnology :
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8
Biotechnology
Quarter 1 – Module 3:
Laboratory Apparatus and
Equipments in Biotechnology
Introductory Message
For the facilitator:
educators both from public and private institutions to assist you, the teacher or
facilitator in helping the learners meet the standards set by the K to 12 Curriculum
This learning resource hopes to engage the learners into guided and
independent learning activities at their own pace and time. Furthermore, this also
aims to help learners acquire the needed 21st century skills while taking into
In addition to the material in the main text, you will also see this box in the
As a facilitator, you are expected to orient the learners on how to use this
module. You also need to keep track of the learners' progress while allowing them to
manage their own learning. Furthermore, you are expected to encourage and assist
ii
For the learner:
The hand is one of the most symbolized part of the human body. It is often
used to depict skill, action and purpose. Through our hands we may learn, create
and accomplish. Hence, the hand in this learning resource signifies that you as a
learner is capable and empowered to successfully achieve the relevant competencies
and skills at your own pace and time. Your academic success lies in your own hands!
This module was designed to provide you with fun and meaningful
opportunities for guided and independent learning at your own pace and time. You
will be enabled to process the contents of the learning resource while being an active
learner.
What I Need to Know This will give you an idea of the skills or
competencies you are expected to learn in the
module.
iii
skills of the topic. You may check the
answers to the exercises using the Answer
Key at the end of the module.
1. Use the module with care. Do not put unnecessary mark/s on any part of the
module. Use a separate sheet of paper in answering the exercises.
2. Don’t forget to answer What I Know before moving on to the other activities
included in the module.
3. Read the instruction carefully before doing each task.
4. Observe honesty and integrity in doing the tasks and checking your answers.
5. Finish the task at hand before proceeding to the next.
6. Return this module to your teacher/facilitator once you are through with it.
iv
If you encounter any difficulty in answering the tasks in this module, do not
hesitate to consult your teacher or facilitator. Always bear in mind that you are
not alone.
v
What I Need to Know
This module is designed and written to help you recognize and give importance
I. Multiple Choice. Choose the letter of the best answer. Write the chosen letter on
a separate sheet of paper. Be honest in answering the questions.
1. Suppose your brother accidentally cut his finger with a broken glass. What will
you do?
a. Continue with what you’re doing.
b. Call your mother and tell her what happened.
c. As first aid, put a medicated plaster around his finger.
d. Call the attention of your neighborhood about what happened.
2. While reading the procedure of an activity, you happen to come across this icon.
What does it mean?
a. It’s a toy.
b. It’s a trademark sign.
c. It’s a sign of an electrician.
d. It’s a sign that you will be using electricity in the activity.
3. Given the following laboratory apparatus: graduated cylinder, reagent bottle, test
tube and watch glass. Which will you use to get the volume of water?
a. test tube
b. graduated cylinder
c. watch glass
d. reagent bottle
4. You are asked by your teacher to get the mass of a fruit that will be used in an
experiment. What apparatus will you use?
a. Florence flask
b. graduated cylinder
c. Erlenmeyer’s flask
d. digital scale
5. You have come across this icon. What does this mean?
a. It is poisonous.
b. It is a metal.
c. It is radioactive.
d. It is a nonmetal.
II. Double Puzzle: Unscramble the ten (10) words below then copy the letters in
the numbered boxes to the box at the bottom with the same number!
Lesson
Laboratory Apparatus and
3 Equipment in Biotechnology
As the world is expanding for its needs and improvements the more human
adapt to these natural changes and phenomena. One of the primary adaptations of
human is to create something out of a living cell, microbes and even studying a
dreadful virus. To do these task, Biotechnology is bound for it.
You’re right! in a laboratory. Here, you can find different laboratory apparatus.
A Biotech’s laboratory is a place of exploration and innovation. Some of the most
breathtaking events in scientific history have occurred in the laboratory. By
harnessing cellular and biomolecular processes, biotechnologist can make advances
and adaptations to technology in various fields. Traditional processes include
utilizing living organisms in their natural form, breeding new living organisms or
altering their genetic makeup. Successful applications of such processes have
resulted in treatment of disease, environmental impact reduction and more efficient
use of natural resources. All these, with the help of the never-gets-old laboratory
instruments.
In the previous lesson, you have learned the different functions of the cell.
Now let us try to remember them by listing differences from one cell function to the
other.
1.
2.
meiosis mitosis
3.
1.
2.
cell respiration photosynthesis
3.
Notes to the Teacher
What’s New
Did you know that the sizes of most plant and animal cells range with dimensions
between 1 and 10 micrometers? So, how did scientists studied something too small to
be seen by the naked eyes? Did they use special instruments or apparatus to do so?
Let us find out as we go deeper!
Observe the pictures carefully. How many laboratory apparatuses and equipment
can you name?
What is It
Using the illustration from the previous page, put a √ on the space provided if you
think that is the name of the material you identified. Put a x if not.
Have you notice that the persons doing laboratory works in the illustrations are
wearing Protective Personal Equipment (PPE) such as gloves, laboratory gown and
googles? Why do they have to wear such PPE?
7. Mortar and pestle- Compliments one another, a bowl, typically made of hard
wood, metal, ceramic, or hard stone, the other one heavy and blunt club-
shaped object. It is used to grind, mesh or crush various substances.
8. Beaker- A cylindrical container with a flat bottom and also have a small spout
(or "beak") to assist in pouring. It is used for stirring, mixing, heating or for
measuring liquids in a variety of laboratory applications.
9. Digital scale- A transparent enclosure with doors so that dust does not collect
and so any air currents in the room do not affect the balance's operation. It is
used to measure the mass of a substance in a remarkably high degree of
precision
10. Bunsen burner- was named after Robert Bunsen, is made entirely of
metal barrel with a collar, air holes, gas intake, gas valve and a stand. When
connected to a source of fuel, it can be lit with a spark thus producing a blue
flame. In biotechnology, it is used commonly for heating, sterilizing and
disinfecting tools and equipment for the transfer of bacterial colonies from one
agar plate to another.
11. Dropper pipette- A glass tube tapered to a narrow point and fitted with
a rubber bulb at the top. It is used is to transfer with precision small volumes
of liquids
13. Goggles or safety glasses - are types of protective eyewear that usually cover
or protect the eye in order to avoid particulate matter, water or chemicals from
striking the eyes.
14. Beaker tongs- designed to grasp and lift laboratory items including hot
evaporating dishes, beakers, crucibles, and other laboratory apparatuses.
15. Ring stand- comprises of a heavy base and a vertical rod, both usually made
of metal. Several accessories, such as clamps of various types and iron rings
can be attached to the rod by thumbscrews at whatever heights and
orientations. The iron stand carries the iron ring with wire gauze when
substances or mixtures are being heated in a beaker or Erlenmeyer flask.
17. Funnel- a Y-shaped equipment described as wide at the top and slim at the
bottom which is used for transferring or guiding liquid or powder into a small
opening.
18. Gloves- Have separate sheaths or openings for each finger and the thumb. As
a form of personal protective equipment (PPE) gloves protect the user's hands
from general hazard. These items may protect from work-related hazards and
risks where the hands are greatly involved in the completion of a task.
19. Lab gown- Knee-length overcoat which is made from white or light-colored
cotton, linen or cotton polyester blend, allowing it to be washed at high
temperature and making it easy to see if it is clean. A lab coat or other
protective clothing should be worn when handling chemicals or biological
materials. The lab coat will protect the biotechnologist’s personal clothing and
exposed skin (such as on arms) from contaminants.
20. Graduated cylinder- It has a narrow cylindrical shape. Each marked line on
represents the amount of liquid that has been measured. It is used to measure
liquid volumes accurately. They are widely used in chemistry and biology labs,
where volume of accurately measured liquids needs to be used.
What’s More
Complete the Table: Based from what you have read on the different laboratory
apparatuses and equipment, complete the table by writing the name or by giving the
function of the shown equipment.
Incubator
It is a standard type of
laboratory equipment that is
used to measure the small
volumes of liquids (usually in
microliters)
Forceps
Centrifuge
Beaker
Digital scale
Graduated cylinder
Thermometer
Test tube
What I Have Learned
What’s on Your Mind!
Share with your friends and family what you have learned from this module! I know that they will
also learn from what you will share to them!
What I Can Do
If you ever see this sign, it is recommended that you wear a face mask and anti-
bacterial gloves when dealing with such living organisms.
In this activity, we will make our own PPE (facemask) made from old t-shirt! Let’s do
this!
Materials needed:
Old t-shirt or rectangular cotton cloth cut approximately
25”x20”
2 ponytails
Procedures:
4. F ol d th e si d es to the
m i ddl e then tu ck.
5. O nce yo u h ave it
secured , t w i st t h e cl o th
ar oun d a nd pul l i t o v er
y our f ace b y pl ac i ng t h e
t w o el a sti cs o ver y o ur
ear s.
2. The desire to maintain a safe laboratory environment for all begins with
_________?
a. prevention c. microbiology
b. ubiquity d. accidents
4. You are heating a piece of glass and now want to pick it up. You should
a. use a rag or paper towels. c. pick up the end that looks cooler
b. use tongs. d. pour cold water on it.
11. Which glass container used to hold, mix, or heat large quantities of solids or
liquids?
a. test tube c. forceps
b. petri dish d. beaker
12.Which laboratory device is used for the separation of fluids, gas or liquid, based
on density?
a. centrifuge c. microscope
b. incubator d. petri dish
14. A heated, insulated box that maintains optimal temperature, humidity, and
other conditions to grow and maintain microbiological cultures or cell cultures.
a. centrifuge c. petri dish
b. mortar and pestle d. incubator