V[(µmol/L)

[S](µmol/L) Método Michaelis-Menten

min-¹
5 22 160

10 39 140

20 65 120

50 102 100
100 120 80
𝑉_𝑚𝑎𝑥/

V
200 135 60 2
40 [𝑆]=𝐾_𝑚
20
0
0 50 100 150
[S]

1/V[(µmol/L)
1/[S](µmol/L) Linewear-Burk
min-¹
0.2 0.0454545455 0.05
0.045
0.1 0.0256410256 0.04 f(x) = 0.196054615694768 x + 0.00609063695784481
0.035 R² = 0.999521357661339
0.05 0.0153846154 0.03
0.02 0.0098039216 1/V 0.025
0.02
0.01 0.0083333333 0.015
0.01
0.005 0.0074074074 0.005
0
0 0.05 0.1 0.15 0.2
m= 0.1961 1/[S]
b= 0.0061
Vmax= 163.93442623 µmol * min^-1
KM= 32.1475409836 µmol

V[(µmol/L) y=mx+b
[S](µmol/L) V0/[S] Eadie-Hofste
min-¹
5 22 4.4 160
f(x) = − 30.1515510953939 x + 158.2
10 39 3.9 140 R² = 0.992444369480791
120
20 65 3.25
100
50 102 2.04
80
V

100 120 1.2 60

200 135 0.675 40
20
m= 30.152 Vmax= 158.22 µmol/L min^-1 0
0 0.5 1 1.5 2 2.5
Km= 30.152 µmol/L
V/[S]

V[(µmol/L) S/V
[S](µmol/L) Hanes-Wolf
min-¹
1.6
1.4 f(x) = 0.00646964125084596 x + 0.1842
R² = 0.999554801463883
1.2
 Hanes-Wolf
5 22 0.22727273 1.6

10 39 0.25641026 1.4 f(x) = 0.00646964125084596 x + 0.1842

R² = 0.999554801463883
20 65 0.30769231 1.2
1
50 102 0.49019608
0.8

[S]/V
100 120 0.83333333
0.6
200 135 1.48148148
0.4
0.2
m= 0.0065
0
b= 0.1843 0 50 100 150
Vmax= 153.846153846 [s]
Km= 28.3538461538

Velocidad
Inicial Método Michaelis-Menten
[S] (mmol/L) [(mmol/)
6
]
5
0.25 0.26
0.33 0.45 4
0.5 0.92 3
V

0.75 1.8
2
1 2.5
2 4.1 1
4 4.8 0
0 0.5 1 1.5 2 2.5 3 3.5 4
[S]

1/V[(µmol/L)
1/[S](µmol/L) Chart Title
min-¹
4 3.8461538462 4.5

3.03030303 2.2222222222 4
3.5
4 1.0869565217
3
3.03030303 0.5555555556
2.5
Axis Title

2 0.4
2
1.333333333 0.243902439 1.5
1
0.5
0
1 1.5 2 2.5 3 3.5 4
Axis Title
do Michaelis-Menten

100 150 200 250

[S]

wear-Burk y=mx+b

4768 x + 0.00609063695784481
339

0.1 0.15 0.2 0.25

1/[S]

Eadie-Hofstee

(x) = − 30.1515510953939 x + 158.215622948378

² = 0.992444369480791

0.5 1 1.5 2 2.5 3 3.5 4 4.5 5

V/[S]

Hanes-Wolf

= 0.00646964125084596 x + 0.184262384737384
0.999554801463883
 Hanes-Wolf

= 0.00646964125084596 x + 0.184262384737384
0.999554801463883

50 100 150 200 250

[s]

Menten

.5 3 3.5 4 4.5

3 3.5 4 4.5