Import Statement A2206179-1

Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Certificate of Origin and Analysis and Import Statement
We declare that the DSMZ culture(s) within this delivery no. A2206179-1, (invoice no. 02206943-1)
to Alife Agrotech, BOGOTA, COLOMBIA, dated 20.07.2022:
DSM-No. Strain Lot.-No. Risk Group

720 Azotobacter vinelandii DSM 720-0814-001 1
2222 Ampelomyces quisqualis DSM 2222-0808-001 1
7 Bacillus amyloliquefaciens DSM 7-0920-001 1
27 Bacillus pumilus DSM 27-0621-001 1
24461 Paenibacillus mucilaginosus 1
28 Lysinibacillus sphaericus DSM 28-0317-001 1
1859 Azospirillum brasilense DSM 1859-0296-001 1
√ √ have been produced in DSMZ laboratories at the address given below

√ are of German preferential origin
√ are biological material
√ do not contain animal derived additives
√ are for laboratory, civil scientific or research and development use only
√ are not for use in humans or animals
√ are free of tissues, serum and plasma of domestic and wild ruminants, swine and equines
√ are not pathogenic
√ are not genetically modified
For microorganisms:
√ are authentic DSMZ cultures derived directly from microorganisms held in the DSMZ
√ have been tested by DSMZ control procedures with respect to purity and identity
For bacteriophages only:

√ are authentic DSMZ preparations derived directly from above bacteriophage preparations held in the DSMZ
√ have been tested by DSMZ control procedures with respect to viability and purity according to appropriate
bacteriophage methodology
√ are not pathogenic
√ are not genetically modified
Viability
For microorganisms:
From all microorganisms provided for deposit, viability and purity are tested by subculturing. Each batch produced is
again checked for viability after preservation, and after intervals scheduled for the different taxa.

For all bacteriophages, viability is tested by applying bacteriophage propagation and titer control. Each batch produced
is checked for viability and after intervals scheduled for bacteriophages.
Purity
Purity of each batch preserved is checked after production.
For microorganisms:
Geschäftsführer/ Braunschweigische Landessparkasse Handelsregister/

Managing Director: Kto.-Nr./Account: 2 039 220 Commercial Register:
Prof. Dr. Jörg Overmann; Bettina Fischer BLZ/Bank Code: 250 500 00 Amtsgericht Braunschweig
Aufsichtsratsvorsitzender/Head of IBAN DE22 2505 0000 0002 0392 20 HRB 2570
Supervisory Board: MR Dr. David Schnieders SWIFT (BIC) NOLADE 2 H Steuer-Nr. 13/200/24030
This check may include microscopical and macroscopical observations or selected physiological, chemosystematic or
molecular based tests.

This check includes analysis of plaque production and plaque morphology on the intended bacterial host and may be
supported, if required, by bacteriophage host spectrum, electron microscopical morphology observations or genome
sequencing.
The DSMZ is not in a position to verify specific properties or applications claimed for a bacteriophage in the literature.
The DSMZ is not responsible for differences between the properties of the bacteriophage deposited in the DSMZ and
properties given in the literature/databases.
The DSMZ does not guarantee that bacteriophage preparations are free of prophages that may have been induced from
the bacterial hosts during bacteriophage propagation.
Authenticity (for microorganisms only)

Each microorganisms is checked by the most significant techniques available for this purpose and the taxon in question.
The following options are available for identity checks:
 morphology and growth behaviour
 physiological properties including API kits or BIOLOG substrate plates
® TM
 selected microorganisms-specific phenotypic/genotypic markers, e.g. resistances, sensitivities, plasmid-

encoded properties
 chemotaxonomic markers such as whole cell fatty acid patterns
 molecular based techniques such as gene sequencing (16S rRNA, house keeping genes), automated
®
RiboPrinting
 MALDI-TOF MS
Storage of ampoules
For microorganisms:
We recommend cold, dry storage in the dark. Freeze-dried cultures should be stored
between 4- 8°C. According to our Terms & Conditions, the material is to be used immediately upon receipt.

Storage of vacuum-dried bacteriophages in glass ampoules:
We recommend dry storage at 4-8°C in the dark.
Interim storage of active preparations of bacteriophages in liquid:

We recommend storage at 4-8°C; cannot be frozen without cryoprotectant.
Our recommended host microorganisms as mentioned in the bacteriophage catalogue information must be used.
General information
All microorganisms and/or bacteriophages are supplied for laboratory use only. According to our Terms & Conditions,
the material is to be used immediately upon receipt.
The batches indicated above passed our quality control system.
Date: 18.07.2022

Dunja Martin
Quality Management


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Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

Certificate of Origin and Analysis and Import Statement

DSM-No. Strain Lot.-No. Risk Group

√ √ have been produced in DSMZ laboratories at the address given below

For bacteriophages only:

For bacteriophages only:

Geschäftsführer/ Braunschweigische Landessparkasse Handelsregister/

For bacteriophages only:

Authenticity (for microorganisms only)

 selected microorganisms-specific phenotypic/genotypic markers, e.g. resistances, sensitivities, plasmid-

For bacteriophages only:

Interim storage of active preparations of bacteriophages in liquid:

The batches indicated above passed our quality control system.

Geschäftsführer/ Braunschweigische Landessparkasse Handelsregister/

Geschäftsführer/ Braunschweigische Landessparkasse Handelsregister/

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