Venipuncture Using Syringe Method

VENIPUNCTURE
using SYRINGE METHOD

Group 4: Liza, Manalo, Nielo
VIDEO #1
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PATIENT
IDENTIFICATION
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Remember this
Verbally identify the patient by asking the

patient to state his or her full name; spell the
last name; or give address, birth date, or unique
identification number.
Source: Phlebotomy by Susan King Strasinger
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Remember this
Compare the response to the requisition form.

Check the patient’s photo identification, if required.
Check the patient’s ID band or patient ID card, if available.

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PATIENT
POSITIONING
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Remember this

Position the patient’s arm so that it is fi rmly supported

and slightly bent in a downward position. This
positioning allows the tubes to fill from the bottom up,
preventing refl ux and additive carryover between tubes.
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TOURNIQUET
application
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Remember this
Always check for possible latex allergies

before applying a latex tourniquet, if a non-
latex tourniquet is unavailable.
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PROCEDURE
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Remember this
Position the tourniquet 3 to 4 inches above

the venipuncture site.
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palpating the
VEIN
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Remember this
Use the tip of the index finger of the

nondominant hand. Never use the thumb for
palpation because it has a pulse.
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cleansing the
site
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Remember this
Cleanse the site with 70% isopropyl alcohol in a

back and forth motion, moving outward 2 to 3 in.,
creating a friction and allow the site to air-dry for
maximum bacteriostatic action.
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Remember this
Do not leave the tourniquet in place for longer

than 1 minute to prevent hemoconcentration
and hemolysis.
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anchoring the
VEIN
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Remember this
Place the thumb of your nondominant hand 1

to 2 inches below and slightly to the side of
the insertion site.
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Remember this
Hold the syringe in your dominant hand with

your thumb on top near the hub and the other
fingers underneath
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Remember this
Smoothly insert the needle into the vein at a

15- to 30-degree angle with the bevel up until
you feel a lessening of resistance
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Remember this
Pulling the plunger of the syringe back too

slowly can cause the blood to begin to clot
before the collection is completed.
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Remember this
Remove the needle smoothly, and

immediately activate the safety device
following the manufacturer’s guidelines.
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Remember this
Do not recap, cut, or bend needles. Quickly

transfer the blood from the syringe to the
evacuated tube to prevent clotting.
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Remember this
Quickly transfer the blood from the syringe to

the evacuated tube to prevent clotting.
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Remember this
Holding the syringe vertically with the blood

transfer device at the bottom, advance the
evacuated tube onto the internal needle in the
blood transfer device.
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Remember this
Keep the tube in a vertical position to ensure

that the tubes fill from the bottom up to avoid
cross-contamination.
Do not push on the plunger.
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Remember this
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Remember this
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Remember this
After the tubes are filled, discard the entire

syringe and blood transfer device into a
sharps disposal container.
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Remember this
Before leaving the patient, label the tubes and

reconfirm the patient’s identification.
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VIDEO #2
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Remember this
Gloves must be worn at all times when

collecting, processing, or testing specimens
and must be changed between patients.
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VIDEO #3
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No requisition
form was shown
- Never collect a
specimen without
a requisition form
to avoid
discrepancies

VENIPUNCTURE
No requisition
form was shown
- Never collect a
specimen without
a requisition form
to avoid
discrepancies

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Did not greet the patient, did not identify

the patient verbally by having their first and
lastname, date of birth.
- We should compare the information on the
patient’s ID band on the requisition form
which is a minimum of two identifiers is
required to avoid discrepancies.

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Asked patient’s
consent
- Consent creates trust
from the patient and it
also reduces the risk
for both patient and
medical technologist/
phlebotomist

VENIPUNCTURE
Asked patient’s
consent
- Consent creates trust
from the patient and it
also reduces the risk
for both patient and
medical technologist/
phlebotomist

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Explained the
procedure

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Explained the
procedure

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Sanitize/wash
hands using the
proper technique

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Sanitize/wash
hands using the
proper technique

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Not wearing
gloves
- Wear gloves
when performing
venipuncture

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Not wearing
gloves
- Wear gloves
when performing
venipuncture

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Not wearing gloves
when palpating the area
- Palpate the area with
index finger of the
nondominant hand in a
vertical and horizontal
to locate a vein

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Not wearing gloves
when palpating the area
- Palpate the area with
index finger of the
nondominant hand in a
vertical and horizontal
to locate a vein

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Ask the patient
to make a fist

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Ask the patient
to make a fist

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Not wearing
gloves when
removing the
tourniquet

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Not wearing
gloves when
removing the
tourniquet

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Assembling the
equipment
before
cleansing the
site

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Assembling the
equipment
before
cleansing the
site

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Wearing a
gloves when
cleansing the
site

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Wearing a
gloves when
cleansing the
site

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Remove the
needle cap and
inspect the
needle

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Remove the
needle cap and
inspect the
needle

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Holding the
syringe
vertically with
the blood
transfer device
at the bottom

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Holding the
syringe
vertically with
the blood
transfer device
at the bottom

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Placed the specimen in
a biohazard bag
- Prepare the specimens
and requisition forms
for transporation to the
laboratory and make
sure to observe special
handling instructions

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Placed the specimen in
a biohazard bag
- Prepare the specimens
and requisition forms
for transporation to the
laboratory and make
sure to observe special
handling instructions

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Did not disposed
the other used
supplies
- Must dispose
the used supplies

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Did not disposed
the other used
supplies
- Must dispose
the used supplies

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VIDEO #4
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REMEMBER THIS!
• Obtain and examine the requisition form.

Source: Strasinger, S., Di Lorenzo M. Phlebotomy Notes. 2nd Edition
APPROVED!
REMEMBER THIS!
• Greet the patient, explain the procedure, and

obtain patient’s consent.
APPROVED
PATIENT IDENTIFICATION
REMEMBER THIS!
■ Verbally identify the patient by asking the patient to state his or her full name; spell the
last name; or give address, birth date, or unique identification number.
■ Compare the response to the requisition form.
■ Check the patient’s photo identification, if required.
■ Check the patient’s ID band or patient ID card, if available.
APPROVED!
REMEMBER THIS!
• Verify the patient’s adherence to pretest

preparation, such as dietary and medication
restrictions.Ask about latex allergies.
APPROVED!
PATIENT POSITIONING
REMEMBER THIS!
• Ask the patient to sit or lie down. Position the

patient’s arm so that it is firmly supported and
slightly bent in a downward position.
APPROVED!
TOURNIQUET APPLICATION
REMEMBER THIS!
• Non-latex tourniquet
• Position the tourniquet 3 to 4 inches above the venipuncture site.
• Do not leave a tourniquet in place for longer than 1 minute because
hemoconcentration and hemolysis can occur.
APPROVED!
TAKE NOTE!
• Though they are all approved, but based on Strasinger’s Phlebotomy notes
after verifying the patient’s adherence to pretest preparation, you should
follow the following steps:
1. Confirm that the patient has removed all objects from his or her mouth.
2. Select the puncturing equipment for the procedure.
3. Then sanitize your hands, apply gloves before positioning the patient’s arm,
and apply the tourniquet.
PALPATING THE VEIN
REMEMBER THIS!
• Use the tip of the index finger of the nondominant hand.

• Use a pushing motion, rather than a stroking motion.
• Feel the vein in both a vertical and horizontal direction.
• Never use the thumb for palpation because it has a pulse.
APPROVED!
REMEMBER THIS!
• Remove the tourniquet after palpating the vein.

Do not leave the tourniquet in place for longer
than 1 minute to prevent hemoconcentration and
hemolysis.
APPROVED
CLEANSING THE SITE
REMEMBER THIS!
• Cleanse the site with 70% isopropyl alcohol in a

back and forth motion, moving outward 2 to 2 in.,
creating a friction and allow the site to air-dry for
maximum bacteriostatic action.
DISAPPROVED!
The phlebotomist
cleaned the area in
a one way slide
vertically.
ASSEMBLING THE EQUIPMENT
REMEMBER THIS!
Assemble the equipment as the alcohol is drying:

■ Select the syringe size according to the volume of blood required for testing.
■ Attach the hypodermic needle to the syringe.
■ Pull the plunger back to ensure that it moves freely and then push it forward to remove any air in
the syringe.
*Position all supplies within easy reach on your nondominant side to avoid reaching over
venipuncture site.
APPROVED!
REMEMBER THIS!
• Sanitize your hands using the proper technique.

Then apply gloves.
DISAPPROVED!
The phlebotomist
touched anything
and didn’t sanitize
her hands before
putting gloves.
REAPPLY THE TOURNIQUET
APPROVED!
REMOVE THE NEEDLE CAP
AND INSPECT THE NEEDLE.
DISAPPROVED!
The
phlebotomists did
not inspect the
needle
REMEMBER THIS!
• Do not touch the puncture site with an unclean

finger. Clean the gloved finger in the same
manner as the venipuncture site, if necessary.
DISAPPROVED!
The phlebotomist
touched the sanitized
site with her un-
sanitized gloved
finger
REMEMBER THIS!
• Smoothly insert the needle into the vein at a 15- to 30-degree angle
with the bevel up until you feel a lessening of resistance. A flash of
blood will appear in the syringe hub when the vein has been entered.
• Pull back the syringe plunger using your nondominant hand to collect
the appropriate amount of blood.
APPROVED!
REMEMBER THIS!
• Release the tourniquet and ask the patient to open

the fist.
• Cover the puncture site with gauze
APPROVED!
REMEMBER THIS!
• Remove the needle smoothly, and immediately

activate the safety device following the manufacturer’s
guidelines. Remove the needle from the syringe and
discard it in a sharps disposal container.
APPROVED!
REMEMBER THIS!
• Attach a blood transfer device to the syringe.

✓Do not recap, cut, or bend needles.
✓Quickly transfer the blood from the syringe to the evacuated tube to prevent
clotting.
✓Keep the tube in a vertical position to ensure that the tubes fill from the bottom
up to avoid cross-contamination.
✓Do not push on the plunger.
DISAPPROVED!
The phlebotomist
removed the cap of
the evacuated tube
and she puled the
plunger
REMEMBER THIS!
• Mix anticoagulated tubes by gentle inversion three to eight

times as soon as they are removed from the transfer device.
• After the tubes are filled, discard the entire syringe and blood
transfer device into a sharps disposal container.
APPROVED!
REMEMBER THIS!
• Before leaving the patient, label the tubes and reconfirm the
patient’s identification.
• Examine the puncture site to verify that the bleeding has
stopped.
• Apply a bandage. Place a bandage over folded gauze for additional pressure.
APPROVED!
REMEMBER THIS!
• Prepare the specimens and requisition forms for transportation to the

laboratory, making sure to observe special handling instructions.
• Dispose of used supplies.
• Thank the patient.
• Remove your gloves.
• Sanitize your hands.
DISAPPROVED!
The phlebotomists
removed her gloves
and sanitized already
without disposing the
materials she used
first.
VIDEO #5
VENIPUNCTURE
Remember this!
 Obtain and examine the requisition form.

 Greet the patient, explain the procedure,
and obtain patient’s consent .

APPROVED!
Patient Identification
REMEMBER THIS!
 Verbally identify the patient by asking the patient to state

his or her full name; spell the last name; or give address,
birth date, or unique identification number.
 Compare the response to the requisition form.
 Check the patient’s photo identification, if required.
 Check the patient’s ID band or patient ID card, if
available.
DISAPPROVED!
The phlebotomist asked
the patient’s name in a
question answerable by
yes or no. He should have
verbally identify the
patient by asking the
patient to state his or her
full name; spell the last
name; or give address,
birth date, or unique
identification number.
SANITIZE HANDS
APPROVED!
PATIENT POSITIONING
REMEMBER THIS!
 Ask the patient to sit or lie down.

 Position the patient’s arm so that it is firmly
supported and slightly bent in a downward
position. This positioning allows the tubes to fill
from the bottom up, preventing reflux and
additive carryover between tubes.
APPROVED!
TOURNIQUET APPLICATION
REMEMBER THIS!
 Non-latex tourniquet (Always check for possible latex
allergies before applying a latex tourniquet, if a non-latex
tourniquet is unavailable.)
 Position the tourniquet 3 to 4 inches above the
venipuncture site.
 Do not leave a tourniquet in place for longer than 1
minute because hemoconcentration and hemolysis can
occur.
APPROVED!
PALPATING THE VEIN
REMEMBER THIS!
 Use the tip of the index finger of the nondominant hand.

 Use a pushing motion, rather than a stroking motion.
 Feel the vein in both a vertical and horizontal direction.
 Never use the thumb for palpation because it has a
pulse.
APPROVED!
REMEMBER THIS!
 Remove the tourniquet after palpating the

vein. Do not leave the tourniquet in place
for longer than 1 minute to prevent
hemoconcentration and hemolysis.
APPROVED!
CLEANSING THE SITE
REMEMBER THIS!
 Cleanse the site with 70% isopropyl alcohol

in a back and forth motion, moving outward
2 to 2 in., creating a friction and allow the
site to air-dry for maximum bacteriostatic
action.
APPROVED!
ASSEMBLING THE EQUIPMENT
APPROVED!
REMEMBER THIS!
 Sanitize
your hands using the proper
technique. Then apply gloves.
DISAPPROVED!
The phlebotomist
did not washed or
sanitised his
hands before
putting on the
gloves
REAPPLY THE TOURNIQUET
DISAPPROVED!
When the phlebotomist

reapplied the tourniquet,
he should have worn the
gloves already
REMOVE THE NEEDLE CAP
AND REAPPLY THE NEEDLE
APPROVED!
REMEMBER THIS!
 Smoothly insert the needle into the vein at a 15- to
30-degree angle with the bevel up until you feel a
lessening of resistance. A flash of blood will appear
in the syringe hub when the vein has been entered.
 Pull back the syringe plunger using your
nondominant hand to collect the appropriate
amount of blood.
APPROVED AND DISAPPROVED
Though the syringe

position was right.
But it would be better
if he also wore the
gloves, therefore it is
also disapproved.
REMEMBER THIS!
 Release the tourniquet and ask the patient to

open the fist.
 Cover the puncture site with gauze

APPROVED!
REMEMBER THIS!
 Remove the needle smoothly, and immediately

activate the safety device following the
manufacturer’s guidelines. Remove the needle
from the syringe and discard it in a sharps
disposal container.
APPROVED!
REMEMBER THIS!
 Attach a blood transfer device to the syringe.

✓ Do not recap, cut, or bend needles.
✓ Quickly transfer the blood from the syringe to the evacuated tube to
prevent clotting.
✓ Keep the tube in a vertical position to ensure that the tubes fill from
the bottom up to avoid cross-contamination.
✓ Do not push on the plunger.
DISAPPROVED!
The phlebotomist removed

the cap of the evacuated
tube and he puled the
plunger
REMEMBER THIS!
 Before leaving the patient, label the tubes and

reconfirm the patient’s identification.
 Examine the puncture site to verify that the
bleeding has stopped.
 Apply a bandage. Place a bandage over folded gauze for
additional pressure.
APPROVED!
REMEMBER!
 Prepare the specimens and requisition forms for

transportation to the laboratory, making sure to observe
special handling instructions.
 Dispose of used supplies.
 Thank the patient.
 Remove your gloves.
 Sanitize your hands.
APPROVED!
SOURCE:
https://www.youtube.com/watch?v=CmD4JnkZzAk&t=3s&
ab_channel=FayeJaurigue
Thank you!
VENIPUNCTURE

Venipuncture Using Syringe Method

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VENIPUNCTURE

using SYRINGE METHOD

Verbally identify the patient by asking the

Compare the response to the requisition form.

Source: Phlebotomy by Susan King Strasinger

Position the patient’s arm so that it is fi rmly supported

Always check for possible latex allergies

Position the tourniquet 3 to 4 inches above

Source: Phlebotomy by Susan King Strasinger

Use the tip of the index finger of the

Cleanse the site with 70% isopropyl alcohol in a

Do not leave the tourniquet in place for longer

Place the thumb of your nondominant hand 1

Hold the syringe in your dominant hand with

Smoothly insert the needle into the vein at a

Pulling the plunger of the syringe back too

Remove the needle smoothly, and

Do not recap, cut, or bend needles. Quickly

Quickly transfer the blood from the syringe to

Source: Phlebotomy by Susan King Strasinger

Holding the syringe vertically with the blood

Keep the tube in a vertical position to ensure

After the tubes are filled, discard the entire

Before leaving the patient, label the tubes and

Source: Phlebotomy by Susan King Strasinger

Gloves must be worn at all times when

Source: Phlebotomy by Susan King Strasinger

Did not greet the patient, did not identify

• Obtain and examine the requisition form.

• Greet the patient, explain the procedure, and

• Verify the patient’s adherence to pretest

• Ask the patient to sit or lie down. Position the

• Use the tip of the index finger of the nondominant hand.

• Remove the tourniquet after palpating the vein.

• Cleanse the site with 70% isopropyl alcohol in a

Assemble the equipment as the alcohol is drying:

• Sanitize your hands using the proper technique.

• Do not touch the puncture site with an unclean

• Release the tourniquet and ask the patient to open

• Remove the needle smoothly, and immediately

• Attach a blood transfer device to the syringe.

• Mix anticoagulated tubes by gentle inversion three to eight

• Prepare the specimens and requisition forms for transportation to the

 Obtain and examine the requisition form.

Source: Strasinger, S., Di Lorenzo M. Phlebotomy Notes. 2nd Edition

 Verbally identify the patient by asking the patient to state

 Ask the patient to sit or lie down.

 Use the tip of the index finger of the nondominant hand.

 Remove the tourniquet after palpating the

 Cleanse the site with 70% isopropyl alcohol

When the phlebotomist

Though the syringe

 Release the tourniquet and ask the patient to

Source: Strasinger, S., Di Lorenzo M. Phlebotomy Notes. 2nd Edition

 Remove the needle smoothly, and immediately

 Attach a blood transfer device to the syringe.

The phlebotomist removed

 Before leaving the patient, label the tubes and

 Prepare the specimens and requisition forms for

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