T or f  What are the other species of taenia that use in
 Man can also be intermediate host of T. solium=
 T- due to similarity of temp
of pig in human but never in
cow
 If human ingest of Taenia egg, they developed?
Cysticercosis- larval infection which can be
erratic
 If human infest Taenia cysticercosis larvae they
developed
Taeniasis- ADULT TAENIA worms in intestine
 ABSTRACT
 What antigen are commonly seen in ELISA and
immunotransher blot for Neurocysticercosis
= Taenia solium
 What taenia species are used with taenia Solium
= Taenia Taenia formis (larvae= Cysticercus
fasciolaris)
 The part of larvae Cysticercus fasciolaris used as
antigen imprinted on elisa
= membrane and scolex
 Cysticercus fasciolaris are extracted in
= Rat (in lab Rattus rattus)
 C. cellulosae are extracted in
= Infected Pig
 The antigen used to compare in Elisa conduct in
___ patients and _controls
=50:50
 What has the highest sensitivity in Elisa antibody
detected using CFM antigen
= IgG , highest sensitivity 94 %
 Consder gold standard by visualization of scolex
= MRI or CT scan
 Specificity for IgG of CFM in ELISA
= 78 %- 90%
 The Immunoreactive bands of 4 antigens are
seen in what band of Western blot
= 40-45, 60-70 KDA
 Infected Pigs with taenia solium require 6
months in order to harvest the larvae,
cysticercus Cellulosae. In the study, Rattus rattus
are used. How many month. Days are stated?
=60 DAYS 1 month
 Introduction
 Causes cysticercosis
= taenia solium cysticerci( Cysticercus cellulosae)
 Consider high morbidity when infected the CNS.
= Neurocysticercosis
 Mainstay diagnosis
=radio immunoassay
 Elisa are adjunct test only and only need >90%
test specificity to consider good candidate. T or F
=T
ELISA AND EITB that have good results
=T saginata, T hydatigena, T. crassiceps
 The study describes
“comparison of membrane and scolex of
Cysticercus fasciolaris to Cysticercosis cellulosae
to detect the IgG and IgM in ElISA in patient with
NCC and healty and infected control participants
using Western blor or Immunoblot”
 Comparison of antigenic bands are done using
what Molecular test?
=Western blot
 METHODS
 Study design
= Case control (Tertiary hospital : King George
medical University, Lucknow india)
 Who is the participants?
Case: 50 with NCC(41 parenchymal lesion and 9
multiple ring lesion)
Ratio male to female of 50 NCC=35:15
Control 50 px
PX with tuberculoma= 36
PX hydatid dx liver= 4
Px with intestinalis taeniaasis=2
PX HEALTHY= 8
 Preparation of antigen of C. fasciolaris
 How do they get the Larvae of taenia taeniasis
They collect stool from India cat containing
gravid segments then they tease it to collect the
eggs. They prepare 1ml= 1000 egg suspension
feed the rattus rattus with 0.2 ml eggsuspension
o And harvest the liver after 60 days.
They Homoginized and sonicated to
form C. fasciolaris antigen.
 Cyst of taenia solium are prepared using?
Butchered pig dissect the cyst same
procedure with C. fasciolaris antigen
 What antigens are produced?
CFM
CFS
CCM
CCS
 Ethical approval used
=principle of Helsinki
 ELISA
 What Specific type of elisa performed?
=Indirect antibody detection
 Microtiter plates were coupled with how many
antigen
=100 uL of antigen
 How many washing step
=25 x
 What is 2nd antibody added that coated with
anti-human IgG and IgM?
 = anti- horseradish peroxidase coated with anti - antigenic antigenic
human IgG and IgM band bands
 Color developer
Cysticercus 6 8
=tetra methyl benzidine
 Absorbance are read at what wavelength
fasciolaris
=450 OD Cysticercus 8 7
 To stop the reaction they add cellulosae
5 N sulfuric acid in 8 SETS of ELISA

 Most of the prominent bands seen in

immunoblot assay are seen in ?
 Inorder to reproduce the results:  40-45,60-70 kDA
Sample run 3x and repeated 2 different days
 Sodium dodecylsulfate electrophoresis
 What buffer are used?
=SDS sodium dodecyl sulfate
 Protein samples are denatures in what buffer?
=a.laemmli sample buffer

3. the sepration of protein are checked using

what staining?
=silver staining==. silver nitirate
 4. when silver bands are already appered “half
visible”it is terminated by
=citric acid solution 5 ml 2.3 M
 immunoblot assay
 bands in silver staining are transferred in
NItROCELLULOSE MEMBRANE
 The nitrocelluosoe memnrane are cut into 4 pcs
then incubatedwith case/control pooled/ control
sera Then it washes And incubated woth anto-
human IgG/igM. Bands are developed using what
chromogen
Diaminobenzidine
 Statistical analysis perfmed
=Sensitivitiy, specificity, NPV, PPV, Student T test

 Based on the result

 What antigen in Table A AND b has the highest
IgG AND iGM detection
CFM> CFS>CCS> ccm
 Range of elisa sensitivity 76 to 94%
 Range of elisa specificity 78-90%
 Highest senti>vity antigen 94% = ccm
 P value result?
 = p value is = 0.80 MEANS ACCEPT THE NULL- 
there is no significant difference
 In Immunoblot analysis complte the table

Membrane Scolex