Department of Education

Region 10- Northern Mindanao

DIVISION OF MISAMIS ORIENTAL

District of jasaan

Jasaan National Highschool1.

Annatto (Atsuete) Seed Extract as Cuticle Tint and Utilized as Antiseptic Wound

Solution

Prepared by;

Risty Mea E. Dangcal

Alyssa Jean T. Ayso

Gertrude Sophia H. Burato

Submitted to:

Almea A. Tongco
 2.

TABLE OF CONTENTS

TITLE …………………………………………………………………………………………………PAGE NO.

Tittle page ……………………………………………………………………….……………………………01

Table of Contents …………………………………………………………………..………………………02

Abstract …………………………………………………………………………………………………………03

Introduction ………………………………………………………………………………………………04-05

Materials/Methodology …………………………………………………….…………………………05-06

a. Materials ………………………………………………………………………………………………05

b. Procedure …………………………………………………………………..………………………05

Review and Literature …………………………………………………………………………….………07

Results and Discussion …………………………………………………………………………….…08-09

a. Hypothesis ………………………………………………………………………………….………08

b. Findings ………………………………………………………………………………………….…08

c. Analysis of Data ………………………………………………………………………..…………9

Conclusions………………………………………………………………………………………………10-11

Acknowledgement ………………………………………………………………………………………….12

References ………………………………………………………………………………………….…………13
 3.

ABSTRACT

The purpose of this project was to determine if Atsuete Seed extract have any

antimicrobial and antioxidant properties and if it can actually heal minor wounds. A

total of 22 grams of atusete seeds are placed and soaked in a 87 ml of Gin (Ginebra

San Miguel). The product was utilized and placed in a plastic container with a lid. The

product is observed each day during the one week experiment. On the first three

days, the colorless gin starts to blend in with the red-orange color of the atsuete

seeds. And on the following 4th and 5th day of the experiment, the scent of the

product also starts to change, its scent has become more stronger than it was before

the experiment. At the end of the experiment (7 days) the product was then

extracted. The atsuete seeds were sifted away from the extract. The data was

analyzed and the conclusion was drawn that the longer the astuete seeds are soaked

in the gin the more effective the wound solution is.

 4.

I. Introduction

Antiseptic solutions, such as chlorhexidine, povidone-iodine, chloroxylenol,

isopropyl alcohol, hexachlorophene, benzalkonium chloride, and hydrogen peroxide.

Are sometimes used to lean infected, or newly contaminated wound. But this

antiseptic solution might damage healthy tissue and delay wound healing. Annatto

seed, an orange-red food coloring or condiment made from the seeds of the achiote

tree (Bixa Orellana), must be a great solution for lesion such as wound, bruises,

scratches and burns. The annatto seed should be soaked in gin for one week as

cuticle tint so we can utilize it as antiseptic wound solution. Gin, a colorless alcohol

beverage made from distilled or redistilled neutral grain spirits flavored with juniper

berries and aromatics. One ingredient that all gins have in common are juniper

berries, it contains chemicals that might decrease inflammation and gas. It might

also be effective in fighting bacteria and viruses. Annatto seed soaked in gin could be

possibly a great and effective as wound solution, as its near, organic, and

convenient.

The Atsuete or Annatto seeds are also a rich source of antioxidants, which are

beneficial for protecting skin cells. However, the cuticle tint which will be our

investigatory project was used to protect new nails from bacteria when they grow

out from the nail root. So, we came up with a study that if we extract this orange-

red Annatto Seed into a Cuticle Tint, we had thought that it'll probably help to

protect the nails from bacteria and can also prevent the wounds into severe

damages, which will be uses as antiseptic organic wound solution. Moreover, it had

also mentioned on our research that the Atsuete seeds has antimicrobial properties
 5.

and antioxidant properties and because of that it can kill various pathogens and

bacteria in your body especially skin.

II. Materials/Methodology

a) Materials

- Annatto seeds (red atsuete)

- Gin

- Plastic Container

b) Procedure

Exact tons (22 grams) of the atsuete seeds were placed inside a slender circular

plastic container. Then the 87 ml of Ginebra San Miguel/Gin was being poured into

the container (not reaching the center of the plastic container) where the atsuete

seeds are. Each day we take picture’s as we observed the changes of what we

conducted until one week. However, after a week we have observe that the colorless

gin turned into red-orange color which means the product totally extracted into

cuticle tint and also it is ready to be utilize as antiseptic wound solution.

6.i
 Day 01 Day 02 Day 03

Day 04 Day 05 Day 06

Day 07

IV. Review and Related Literature

 According to Hindawi, Bixa Orellana popularly known as “urucum” has been used

by indigenous communities in Brazil and other tropical countries for several biological

applications, which indicates its potential use as an active ingredient in

pharmaceutical products. Annatto (Bixa Orellana) is an important colorant

domesticated in the neotropics, although it is not clear were or from which wild

populations. Annatto food coloring has a long history of use in the food industry for

the coloring of wide range of food commodities (Michael Scotter, 2009). Annatto is a

yellow-orange colorant that is widely used in the food industry, particularly in the

dairy industry.

Published research studies in animals indicate that various extracts of Bixa leaves

exhibit antioxidant, broad antimicrobial (anti- bacterial and antifungal) anti-

inflammatory, analgesic, hypoglycemic, and antidiarrheal activities. Cuticle tends to

get dry, damaged, and infected. To avoid this, it is important to use a nail cuticle tint

but instead of using cuticle tint with benzalkonium Chloride we came up with a

project entitled as “Annatto Seed Extracted as Cuticle Tint and Utilized as antiseptic

Wound Solution”.

V. Results and Discussion

A. Hypothesis

• Extracts taken from annatto (atsuete) seeds have the potential to be made into an

antiseptic wound solution.

• If the annatto (atsuete) seeds are soaked in gin for a week or more, then the

wound solution is more effective.

B. Findings

The result of the project revealed that atsuete and gin can be a source of Povidone

iodine and/or chlorhexidine gluconate as an antiseptic wound solution to be used as

prevention and treatment of infection and, therefore, increased rate of the healing

process which be advantageous because this kind of antiseptic wound solution would

be really easy to make.

We tested the product by dividing the product into two. In set A, we applied the

solution on the wound on it's third day of the one week experiment. The wound took

5 days to fully heal. And in set B, we applied the solution on the wound on it's 8th

day of the experiment. The wound only took 3 days to fully heal. This explains that

the longer the annatto (atsuete) seeds are soaked in gin, the more effective the

product is.

9.
 Days 3rd Day of Experiment 8th Day of Experiment
Day 1

Day 2

Day 3

Day 4

Day 5

C. Analysis of Data

For better results, one should follow the method of preparation or suggested

quantities of atsuete seeds and gin. This was shown in the list of table:

Materials Amount
Atsuete seeds 22 grams
Gin 87 ml

10.
VI. Conclusions

We, therefore conclude that atsuete seed that are utilized together with gin can be

very useful in terms of wound healing properties. Gin is made from juniper berries,

little dark purple nuggets with superfood powers. These berries can help fight

infection. Meanwhile, Atusete or Annatto seeds can be applied to speed the healing

process. It can also reduce the appearance of scars. Also, its natural antimicrobial

nature can help prevent infections. Firstly, the Atsuete seeds are also a rich source of

antioxidants, which are beneficial for protecting skin cells. However, Gin was

originated as a medicinal liquor from juniper berries. It has this exquisite and strong

scent. While conducting our project, we observed that the color and texture of the

atsuete seeds dissolves by the liquid coming from the Gin. We've aggregate this two

subject together and they become an orange-red fluid. Secondly, Atsuete seeds has

antimicrobial and antioxidant properties extracted with the used of Gin and resulted

as cuticle tint that can also utilize as antiseptic wound solution. Hence, after applying

the result of our project in actual, we've noticed that the liquid helps to protect and

clean new nails from bacteria especially when they grow out from the nail root.

Thirdly, Atsuete seeds can promote healing. Applying our project also in wounds, the

process of healing will be expedited. We've observed that the liquid (result of our

project) also helps the wound area from getting damaged and infected. In our

observation, it'll only not take 3-4 days to be fully healed. But since Gin is an

exquisite and strong kind of liqour, it is tender to the touch of your skin which was

congruent to the feeling of medicinal alcohol being applied on your wound. You can

11
still feel pain and sore after applying our project but it will only take few seconds to

perceive. If you combined Atsuete seeds and Gin, the result will be an orange-red

liquid. But this liquid can be a cuticle tint that will helps to protect your nails and

skin. And this orange-red liquid that uses as cuticle tint can also utilize as antiseptic

wound solution. Therefore, our project can also be applied and used by others.

However, some people might have an inflammatory reaction or allergy to topical

products. In any case, atsuete and gin has also this side effects resulting of itching,

allergic reactions, or other serious problems on skin. But while conducting our

project our observations helps us conclude that the results of our project answered

almost all of our hypothesis supposing our project to be authentically effective

12
VII. Aknowledgement.

The pleasure that follows the sucessful completion of a project would remain

incomplete without a word of gratitude for the people and without whose

cooperation the achivement would remain a distant goal. It is not a mere formality to

place a record the tireless efforts, ceaseless cooperation, constant guidance and

encouragement of the people closely assosiated with the project but a distant

necessity for the authencity and readibility of the project.

We are thankful to Mrs. Almae A. Tongco, for her scholarly guidance, advise and

encouragement.

Finally, we are indebted to our family, friends and those people who had helped us in

completing this project.

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VIII. References

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sciencedirect.com. "Phytochemistry, biological activities and potential of annatto in

natural colorant production for industrial applications". Last modified March 27, 2022.

https://www.sciencedirect.com/science/article/pii/S2090123215001101

hindawi.com. "Traditional uses, chemical constituents, and biological activities of Bixa

orellana". Last modified March 11, 2022.

https://www.hindawi.com/journals/tswj/2014/857292/

sciencedirect.com. "Antimicrobial activity of the leaves and seeds of Bixa orellana".

Last modified March 28, 2022.

https://www.sciencedirect.com/science/article/pii/S0367326X02002897

researchgate.net. "Safety and Efficacy of Bixa orellana (Achiote, Annatto) Leaf

Extracts". Last modified March 11, 2022. https://www.researchgate.net/profile/Sid-

Stohs/publication/259395225_Safety_and_Efficacy_of_Bixa_orellana_Achiote_Annatt

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orellana-Achiote-Annatto-Leaf-Extracts.pdf

plantsjournal.com. "Burn wound healing potential of Bixa orellana Linn [Bixaceae]

leaf extracts on albino mice". Last modified March 27, 2022.

https://www.plantsjournal.com/archives/2016/vol4issue1/PartB/3-6-2.pdf