Primer-BLAST Primer designing tool

NCBI/ Primer-BLAST : results more...

Input PCR template

 
Range
1 - 380
Specificity of primers
primers may not be specific to the input PCR template as targets were found in
selected database:All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS,
GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) (Organism limited
to Aedes aegypti)
Summary of primer pairs

Detailed primer reports

Primer pair 1
 Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward
GCCCCTGTCTCAGGGTCTAT Plus 20 29 48 60.48 60.00%
primer
Reverse
TATGACCCGTTTAATCCCCA Minus 20 300 281 60.01 45.00%
primer
Internal
Plus
oligo
Product
272
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 .................... 48

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 .................... 48

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44
Reverse primer 1 TATGACCCGTTTAATCCCCA 20
Template 296 .................... 277

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T..C.............. 48

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................T.. 281

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A........... 286
>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 30 .................... 49

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 301 ........A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

Products on target templates

Primer pair 2
Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward AAGGCTCATGTAGAAGCCC
Plus 20 14 33 60.60 55.00%
primer C
Reverse
TATGACCCGTTTAATCCCCA Minus 20 300 281 60.01 45.00%
primer
Internal
Plus
oligo
Product
287
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................... 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................... 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................T.. 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................T.. 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................T.. 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................T.. 281
>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................T.. 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A........... 286

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 15 .................... 34

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 301 ........A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 287
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

Products on target templates

Primer pair 3
Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward
GCTCATGTAGAAGCCCCTGT Plus 20 17 36 59.31 55.00%
primer
Reverse
TATGACCCGTTTAATCCCCA Minus 20 300 281 60.01 45.00%
primer
Internal
Plus
oligo
 Product
284
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 17 .................... 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 17 .................... 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 17 ..............T..... 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 17 ..............T..... 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 13 .................... 32

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 13 .................... 32

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 13 .................... 32

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 17 ..............T..C.. 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................T.. 281

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 17 ..............T..... 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A........... 286

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 18 .................... 37

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 301 ........A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 284
Forward primer 1 GCTCATGTAGAAGCCCCTGT 20
Template 22 .................... 41
Reverse primer 1 TATGACCCGTTTAATCCCCA 20
Template 305 ..C.....A........... 286

Products on target templates

Primer pair 4
Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward
GCCCCTGTCTCAGGGTCTA Plus 19 29 47 60.21 63.16%
primer
Reverse
TATGACCCGTTTAATCCCCA Minus 20 300 281 60.01 45.00%
primer
Internal
Plus
oligo
Product
272
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 29 ................... 47

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 29 ................... 47

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 29 ..T................ 47

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 29 ..T................ 47

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 25 ................... 43

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 25 ................... 43

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 25 ................... 43

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 29 ..T..C............. 47

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................T.. 281

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 29 ..T................ 47

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A........... 286

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 30 ................... 48

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 301 ........A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52
Reverse primer 1 TATGACCCGTTTAATCCCCA 20
Template 305 ..C.....A........... 286

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 272
Forward primer 1 GCCCCTGTCTCAGGGTCTA 19
Template 34 ................... 52

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

Products on target templates

Primer pair 5
Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward CATGTAGAAGCCCCTGTCTC
Plus 21 20 40 60.26 52.38%
primer A
Reverse
TATGACCCGTTTAATCCCCA Minus 20 300 281 60.01 45.00%
primer
Internal
Plus
oligo
Product
281
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 20 ..................... 40

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 20 ..................... 40

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45
Reverse primer 1 TATGACCCGTTTAATCCCCA 20
Template 305 .................... 286

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 20 ...........T......... 40

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 20 ...........T......... 40

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 16 ..................... 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 16 ..................... 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 16 ..................... 36

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277
>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 20 ...........T..C...... 40

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................T.. 281

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 20 ...........T......... 40

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A........... 286

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 21 ..................... 41

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 301 ........A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 281
Forward primer 1 CATGTAGAAGCCCCTGTCTCA 21
Template 25 ..................... 45

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

Products on target templates

Primer pair 6
Sequence (5'->3') Strand Length Start Stop Tm GC%
on
 template
Forward
GCCCCTGTCTCAGGGTCTAT Plus 20 29 48 60.48 60.00%
primer
Reverse
ATGACCCGTTTAATCCCCA Minus 19 299 281 60.00 47.37%
primer
Internal
Plus
oligo
Product
271
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 .................... 48

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 ................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 .................... 48

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 ................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 ................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 ................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 ................... 286

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 .......A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 .......A........... 281

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 295 ................... 277
>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 295 ................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 295 ................... 277

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T..C.............. 48

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 ................T.. 281

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 .......A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .......A........... 286

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 30 .................... 49

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 300 .......A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .......A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 ............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .C.....A........... 286

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 271
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .C.....A........... 286

Products on target templates

Primer pair 7
Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward
GCCCCTGTCTCAGGGTCTAT Plus 20 29 48 60.48 60.00%
primer
Reverse GACCCGTTTAATCCCCAATA
Minus 21 297 277 59.90 42.86%
primer A
Internal
Plus
oligo
Product
269
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 .................... 48

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 297 ..................... 277

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 .................... 48

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 297 ..................... 277

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 ..................... 282

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 ..................... 282

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 ..................... 282

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 297 .....A............... 277
>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 297 .....A............... 277

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 293 ..................... 273

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 293 ..................... 273

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 25 .................... 44

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 293 ..................... 273

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T..C.............. 48

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 297 ..............T...... 277

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 29 ..T................. 48

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 297 .....A............... 277

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 .....A............... 282

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 30 .................... 49

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 298 .....A............... 278

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 .....A.....C......... 282

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 ..........TC......... 282

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 .....A............... 282

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 .....A............... 282

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 .....A............... 282

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 269
Forward primer 1 GCCCCTGTCTCAGGGTCTAT 20
Template 34 .................... 53

Reverse primer 1 GACCCGTTTAATCCCCAATAA 21

Template 302 .....A............... 282

Products on target templates

Primer pair 8
Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward TAAGGCTCATGTAGAAGCCC
Plus 21 13 33 60.59 52.38%
primer C
Reverse
TATGACCCGTTTAATCCCCA Minus 20 300 281 60.01 45.00%
primer
Internal
Plus
oligo
Product
288
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 13 ..................... 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 13 ..................... 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 13 ..................T.. 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 13 ..................T.. 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 9 ..................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 9 ..................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 9 ..................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 13 ..................T.. 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................T.. 281

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 13 ..................T.. 33

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A........... 286

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 14 ..................... 34

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 301 ........A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38
Reverse primer 1 TATGACCCGTTTAATCCCCA 20
Template 305 ........A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 288
Forward primer 1 TAAGGCTCATGTAGAAGCCCC 21
Template 18 ..................... 38

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

Products on target templates

Primer pair 9
Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward AAGGCTCATGTAGAAGCCC
Plus 20 14 33 60.60 55.00%
primer C
Reverse
ATGACCCGTTTAATCCCCA Minus 19 299 281 60.00 47.37%
primer
Internal
Plus
oligo
Product
286
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target

Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................... 33

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 ................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................... 33
Reverse primer 1 ATGACCCGTTTAATCCCCA 19
Template 299 ................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 ................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 ................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 ................... 286

>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................T.. 33

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 .......A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................T.. 33

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 .......A........... 281
>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 10 .................... 29

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 295 ................... 277

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 10 .................... 29

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 295 ................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 10 .................... 29

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 295 ................... 277

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................T.. 33

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 ................T.. 281

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 14 .................T.. 33

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 299 .......A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .......A........... 286

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 15 .................... 34

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 300 .......A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .......A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 ............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .C.....A........... 286
>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 286
Forward primer 1 AAGGCTCATGTAGAAGCCCC 20
Template 19 .................... 38

Reverse primer 1 ATGACCCGTTTAATCCCCA 19

Template 304 .C.....A........... 286

Products on target templates

Primer pair 10
Strand
Sequence (5'->3') on Length Start Stop Tm GC%
template
Forward
TGATTGCCTAAGGCTCATGT Plus 20 5 24 58.33 45.00%
primer
Reverse
TATGACCCGTTTAATCCCCA Minus 20 300 281 60.01 45.00%
primer
Internal
Plus
oligo
Product
296
length
Product Tm
Product Tm
-
min(OLIGO
Tm)
Exon
junction
Total intron
size
Products on intended target
Products on allowed transcript variants

Products on potentially unintended templates

>EU650417.1 Aedes aegypti haplotype H13 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 5 .................... 24

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>EU650415.1 Aedes aegypti haplotype H11 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 5 .................... 24

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................... 281

>AF334846.1 Aedes aegypti isolate 6 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF203356.1 Aedes aegypti isolate ND46b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286

>AF334864.1 Aedes aegypti isolate 24 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .................... 286
>EU650412.1 Aedes aegypti haplotype H8 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 5 .................... 24

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU650411.1 Aedes aegypti haplotype H7 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 5 .................... 24

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EF153754.1 Aedes aegypti haplotype 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 1 .................... 20

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EF153745.1 Aedes aegypti haplotype 2 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 1 .................... 20

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>AY906842.1 Aedes aegypti clone 8 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 1 .................... 20

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 296 .................... 277

>EU650416.1 Aedes aegypti haplotype H12 NADH dehydrogenase subunit 4 (ND4) gene,
partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 5 .................... 24

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 .................T.. 281

>EU650413.1 Aedes aegypti haplotype H9 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 5 .................... 24

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 300 ........A........... 281

>EU446273.1 Aedes aegypti isolate 9 NADH dehydrogenase subunit 4 (ND4) gene, partial
cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A........... 286

>EU446270.1 Aedes aegypti isolate 11 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 6 .................... 25

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 301 ........A........... 282

>EU446268.1 Aedes aegypti isolate 13 NADH dehydrogenase subunit 4 (ND4) gene,

partial cds; mitochondrial
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ........A.....C..... 286

>AF334865.1 Aedes aegypti isolate 25 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .......T............ 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 .............TC..... 286

>AF334841.1 Aedes aegypti isolate 1 NADH dehydrogenase subunit 4 gene, partial cds;
mitochondrial gene for mitochondrial product
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203362.1 Aedes aegypti isolate ND49b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203361.1 Aedes aegypti isolate ND49a NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

>AF203360.1 Aedes aegypti isolate ND48b NADH dehydrogenase subunit 4 gene, partial
cds; mitochondrial gene for mitochondrial product
product length = 296
Forward primer 1 TGATTGCCTAAGGCTCATGT 20
Template 10 .................... 29

Reverse primer 1 TATGACCCGTTTAATCCCCA 20

Template 305 ..C.....A........... 286

Products on target templates