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Commonly Used Stock Solutions & Buffers (I)

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Stock Solutions
10M Ammonium Acetate: 1M KCl:
Ammonium acetate 385.4g KCl 74.6g
H2O to 500ml H2O to 1 liter
1M CaCl2: 1M MgCl2:
CaCl2x2H2O 147g MgCl2 x6H2O 20.3g
H2O to 1 liter H2O to 100ml
100X Denhardt Solution: 1M MgSO4:
Ficoll 400 10g MgSO4 x7H2O 24.6g
Polyvinylpyrrolidone 10g H2O to 100ml
Bovine serum albumin 10g 5M NaCl:
H2O to 500ml NaCl 292g
Filter sterilize and store at -20°C in 25ml aliquots H2O to 1 liter
1M Dithiothreitol (DTT): 10M NaOH:
DTT 15.45g NaOH 400g
H2O to100ml H2O to 1 liter
Store at -20°C
1M Tris-HCl [tris(hydroxymethyl)aminomethane]:
0.5M EDTA (ethylenediamine tetraacetic acid) Tris base 121g
(pH 8.0): H2O to 800ml
Na2EDTAx2H2O 186.1g Adjust to desired pH with concentrated HCl.
H2O to 700ml Mix and add H2O to 1 liter
Adjust pH to 8.0 with 10M NaOH (~50ml)
H2O to 1 liter 3M Sodium Acetate (pH 5.2 and 7.0):
Sodium acetate. 3H2O 408.1g
10mg/ml Ethidium Bromide: H2O to 800ml
Ethidium bromide 0.2g Adjust the pH to 5.2 with glacial acetic acid
H2O to 20ml or
Mix well and store at 4°C in dark. adjust the pH to 7.0 with dilute acetic acid.
CAUTION: Ethidium bromide is a mutagen and must
H2O to 1 liter
be handled carefully.

LITHUANIA FERMENTAS AB, fax: +370 2 602142; info@fermentas.lt

CANADA MBI FERMENTAS INC., fax: +1 800 4728322, +1 905 3333135; info@fermentas.com
GERMANY MBI FERMENTAS GMBH, fax: +49 622753694; fermentas@t-online.de
USA FERMENTAS INC., fax: +1 800 4728322, +1 905 3333135; info@fermentas.com
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Commonly Used Stock Solutions & Buffers (II)

Buffers (per liter):

10X Stock Phosphate-buffered Saline (PBS): 5X SDS Electrophoresis Buffer
Buffer:
NaCl 80g Tris base 15.1g
KCl 2g Glycine 72.0g
Na2HPO4 14.4g SDS 5.0g
KH2PO4 2.4g H2O to 1 liter
H2O to 800ml Dilute to 1X or 2X for working solution, as appropriate.
HCl to pH 7.4 Store up to 1 month at 0°C to 4°C.
H2O to 1 liter Do not adjust the pH of the solution, as the solution is
20X SSC: pH 8.3 when diluted.
NaCl 175.3g (3M) 50X TAE (Tris/acetate/EDTA) Electrophoresis Buffer:
Na3citrate x H2O 88.2g (0.3M) Tris base 242g
H2O to 800ml Glacial acetic acid 57.1ml
Adjust pH to 7.0 with 1M HCl 0.5M EDTA (pH 8.0) 100ml
H2O to 1 liter H2O to 1 liter
20X SSPE: Adjust pH to ~8.5
NaCl 175.3g (3M) 10X TBE (Tris/borate/EDTA) Electrophoresis Buffer:
NaH2PO4 x H2O 27.6g (0.2M) Tris base 108g
Na2EDTA 7.4g (0.02M) Boric acid 55g
H2O to 800ml 0.5M EDTA (pH 8.0) 40ml
Adjust pH to 7.4 with 10M NaOH H2O to 1 liter
H2O to 1 liter 10X TPE (Tris/phosphate/EDTA) Electrophoresis Buffer:
Tris base 108g
Phosphoric acid (85%) 15.5ml
0.5M EDTA (pH 8.0) 40ml
H2O to 1 liter
TE (Tris/EDTA) Buffer, pH 7.4, 7.6 or 8.0:
1M Tris, pH 7.4, 7.6 or 8.0 10ml (10mM)
0.5M EDTA (pH 8.0) 2ml (1mM)
H2O to 1 liter

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