Gene Editing & CRISPR/Cas9

https://www.drugtargetreview.com/news/56842/researchers-create-new-cas9-variant-to-reduce-off-target-crispr-edits/

D. Listyorini, Ph.D

1) Department of Biology; 2) Department of Biotechnology

State University of Malang
Last Edited: Sept. 20, 2021
 Concept:

Memperbaiki kondisi genomik suatu

Dwi Listyorini, Ph.D. Sept. 2021

organisme melalui introduksi sekuen
normal suatu gen

2
 CRISPR-Cas9
• Merupakan bagian dari sistem imun bakteri dan arkhea
terhadap infeksi virus dan plasmid dari virus lain.

Dwi Listyorini, Ph.D. Sept. 2021

• Bakteri mengambil Sebagian DNA virus yang
menginfeksinya untuk membuat segmen DNA yang
kemudian disebut CRISPR arrays

• CRISPR arrays menjadikan bakteri yang bersangkutan

mengingat virus-virus (dan yang berkerabat dekat) yang
bisa menginfeksinya.

3
 CRISPR-Cas9 System Structure

Dwi Listyorini, Ph.D. Sept. 2021

4
https://www.researchgate.net/publication/260109346_CRISPR_Is_an_Optimal_Target_for_the_Design_of_Specific_PCR_Assays_for_Salmonella_enterica_Serotypes_Typhi_and_Paratyphi_A/figures?lo=1
 CRISPR-Cas9
• CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats
• Cas9: CRISPR-associated protein 9

Dwi Listyorini, Ph.D. Sept. 2021

5
 Dwi Listyorini, Ph.D. Sept. 2021
6
Theranostics 2017, Vol. 7, Issue 18
Various targets of Genome Editing

Dwi Listyorini, Ph.D. Sept. 2021

7
http://lifvisindi.hi.is/crispr
 CRISPR: Embryonic Editing

Dwi Listyorini, Ph.D. Sept. 2021

 CRISPR-Cas9
for Various Health Problems

Dwi Listyorini, Ph.D. Sept. 2021

9
https://www.sciencedirect.com/science/article/abs/pii/S1525001620304858
CRISPR-Cas9 for Cancer Therapy

Dwi Listyorini, Ph.D. Sept. 2021

10
https://science.sciencemag.org/content/367/6481/eaba7365
CRISPR for COVID19
and
other Viral Disease?

Dwi Listyorini, Ph.D. Sept. 2021

Tell us
your idea

11