Assignment – 1:

Research Assignment

1- This will be the individual assignment.

2- You have to download two research articles from google scholar on the assigned topics
in addition to one paper provided.
i. (Note: Write the topic name in google scholar search bar and download
unpaid articles therefrom)
3- A data extraction sheet is given below for you to do appropriate working.
4- Write an abstract to your work

Assignment on Data Extraction (Articles)

Name: MUHAMMAD ALEEM, Roll No. 35, Class: BIOTECHNOLOGY

Data Extraction Form

Article no: 02
Title: Ultrahigh resolution metabolomics for S-containing metabolites

Author (s): Nakabayashi, Ryo and

Publication Date: 2017
Saito, Kazuki
Publisher (if any): Elsevier Country: JAPAN
Journal: Current opinion in Volume: Issue Number: Page no:
biotechnology 43 12 8--16
IF: 10.1016 Citations: 19
Keywords/definitions: bioactive properties, genetically modified organisms, health
promotion, ions, mass spectrometry, metabolites, metabolomics, sulfur, synthetic biology
Objective of the Study: (Conceptual Framework):
Metabolomic analysis targeted at S-omics is performed for S-metabolites containing the S (om-
metabolites). The distinction between stable isotopes of 32S and 34S is key to S-omics. The metabolite
asparagine found in Asparagus officinalis uses S-omics'-omics streamlines to obtain S-metabolites
from metabolome resources.

Findings:
The advent of the genome planning period greatly increases the chances of biological research aimed at
improving the production of active metabolism in heterologous host. Different types of sulfur (S) that
contain metabolites (S-metabolites) are known to contain organisms and health-promoting structures,
but the discovery and distribution of chemicals using mass spectrometry-based metabolomics has been
difficult. In this review, we highlight recent developments in targeted metabolomic analysis of S-
metabolites (S-omics) in plants using ultrahigh resolution mass spectrometry. The use of direct and
indirect weight loss between 32S-containing ion-containing ions and its 34S isotopic ions is using a
completely new method for synthesizing S-metabolites. Finally, we discuss the discovery of S-omics of
synthetic biology.

Methodology:
Recent research focuses on stable isotope labeling using 15N, 18O, and 34S. This technique enhances
the signal intensity of the resulting isotopic ions (e.g., 15N). Therefore, a comparative analysis of non-
stable and stable isotope-labeled data can comprehensively extract ions containing N, O, and S.
Recently, research targets are changing from model organisms to those of economic or medical
importance. Although some stable isotope-labeled plants are commercially available in several plant
species (e.g., Iso Life), there are still limitations to prepare these in general laboratory experiments. A
new method was required applicable profiling S-metabolites in any plants.
S-Omics aims to characterize 32S-containing monoisotopic ions (S-ions) using the differences between
S-ions and 34S isotopic ions in ultrahigh resolution metabolome data. In S-omics, the use of native S-
ions and their counterpart 34S isotopic ions are prioritized to cover a wide range of plants such as
vegetables or medicinal plants, which cannot be easily labeled by the use of stable isotopes. One
current limitation remaining with this approach is that only relatively abundant S-ions can be extracted
from the metabolome data. Finally, chemical assignments of extracted S-ions are performed to
determine molecular formulae or putative/complete structures.

What you have learnt from the research paper

S-metabolites has not yet been fully developed. Therefore, it seems highly probable that the
experiments of high-spectral spectral weight using carefully calculated weight variations will find
interesting S-metabolites in metabolome resources. S-Omics has the potential to be a promising
approach to S-metabolite-based synthetic biology.