Novel Humectant having “Long-Lasting Moisturizing Effect”

Glycerin Derivative of Arginine

”Long-Lasting Moisturizing Effect” for both Skin and Hair

13th edition August 20, 2019

1
What’s Amitose R ?

“Keeping moisture” is closely

related to skin and hair beauty.

[Relation between skin condition and moisture] [Relation between hair condition and moisture]

High
Water (moisture) content of

High

Secondary transpiration
100
(% base of healthy skin)

6.0
stratum corneum

moisture (%)
80
5.5

Moisture
Moisture
60
5.0
40

20 4.5
～
～
Low Low
0 0
Healthy skin Damaged skin Healthy hair Damaged hair

※ Figure of skin surface

2
What’s Amitose R ?
Structural design of Amitose R
Amitose R is a new ingredient made from
the combination of Glycerin and Arginine.

Glycerin Arginine
High moisturizing effect Moisturizing effect “Long-Lasting Moisturizing Effect”
Mostly used as Amino acid,
moisturizing ingredient Natural moisturizing factor (NMF)
High moisturizing effect
Adsorption power High adsorption power
“Hydrogen bond” “Ionic bond”
by hydroxyl groups in molecule by guanidino group in molecule High sustained effect
from high adsorption power
Synergy effect of
“Hydrogen bond” and “Ionic bond”

Glyceryl group Derivation from Arginine

※Strength of binding energy : Hydrogen bond < Ionic bond

3
What’s Amitose R ?
Chemical structure and characteristics

Glyceryl group Derivation from Arginine

Moisturizing Adsorption Long-Lasting

effect power Moisturizing Effect
Amitose R closely adsorbs to skin and hair.
As a result, moisturizing effect continues for a long time.
Amitose R contributes to good sensory feeling; “Moist feeling” but “Non sticky feeling”.
Amitose R gives skin and hair flexibility.
Amitose R is safe for use.

4
Effect of Amitose R
Mechanism of “Long-Lasting Moisturizing Effect” for skin and hair
Amino acid composition of
human keratin K1/K10
Acidic amino acids
(anionic functional group)

Basic amino acids Amitose R

Neutral
amino acids

Ionic bond
Hydrogen bond

Skin or Hair
R ： Side chain of amino acids

Amitose R closely adsorbs to skin (stratum corneum) and damaged parts of hair (anionic feature)
thanks to “Ionic bond” from guanidino group in arginine in addition to “Hydrogen bond” from hydroxyl
group in glyceryl group.
That is why it is difficult to rinse off, and moisturizing effect continues for a long time. In case of hair
care, Amitose R penetrates into inner of hair because of its low-molecular weight, and keeps moisture.

5
“Long-Lasting Moisturizing Effect” for both Skin and Hair

All-in-One Care

For
Scalp
care

6
Effect for skin

Validation of skin conditioning effect

7
Effect for skin
“Long-Lasting Moisturizing effect” for skin
in vivo test (W/W%)

Volunteers Skin toner Control Amitose R

5 volunteers (20’s to 30’s) Amitose R - 1.0

Glycerin* 0.1 -
Measurement (before use) Arginine* 0.2 -
Water Up to 100 Up to 100
The volunteers’ inner lower arm was washed, acclimated ※ The molar mass was taken into consideration when
and the water content was measured. formulating the control
(W/W%)
Application Face-wash Common
Myristic Acid 7.0
On the lower inner arm a solution of 1.0% Amitose R and a
control were applied and left 15 minutes. Palmitic Acid 10.0
Stearic Acid 10.0
Lauric Acid 2.0
Washing
Butylene Glycol 10.0
The inner lower arm was washed with a face-wash, then Glycerin 3.0
rinsed with running water for a minute and wiped off. PEG-150 Stearate 2.0
Cocamide Methyl MEA 2.0
Measurement (after use) Phenoxyethanol (90%) /
0.5
Ethylhexylglycerin (10％)
Potassium Hydroxide 5.3
Water Up to 100

8
Effect for skin
“Long-Lasting Moisturizing effect” for skin

Amitose R shows a higher and longer lasting moisturizing effect

Measurement of water content in the epidermal stratum corneum (in vivo test)
30
# p<0.1 (t-test) High
Amitose R
25 Control
Not applied
20
Capacitance()

Moisture
15

10 * * *
5

0
Low
-5
Application After washing 15min 30min 45min 60min
[Method] The inner lower arm of 5 volunteers (20’s to 30’s) was washed, acclimated 20 min (20±2 deg C, 50±5%) and water content in the stratum corneum
measured. A solution of 1% Amitose R or a control of arginine and glycerin (molar mass of 10.6 μmol/L, identical to Amitose R) was applied and left 15
min. The surface was then washed 30 sec. with a lathering face-wash diluted at 5% and rinsed with running water 1 min. The water content was
measured with Corneometer CM-825 (Courage+ Khazaka electronic GmbH) immediately after washing and 15, 30, 45 and 60 min after.
[Conditions] Temperature: 20±2 deg C, humidity 50±5%

9
Effect for skin
“Long-Lasting Moisturizing effect” for skin

In vivo test (W/W%)

Volunteers Gel Control Amitose R

Amitose R - 1.0
13 volunteers (20’s to 40’s)
Polyacrylamide, C13-14
2.0 2.0
Isoparaffin, Laureth-7, Water
Evaluation (before use) Phenoxyethanol 0.5 0.5
Citric Acid / Sodium Citrate * q.s. q.s.
Water Up to 100 Up to 100
Application for 6 weeks * Adjusted to pH5.5

Volunteers applied gel formulae twice a day on their cheeks.

Evaluation (after use)

Test-1; Measurement of water content

in the epidermal stratum corneum

Test-2; Evaluation of suppressing effect

of multilayer desquamation of the stratum corneum

Test-3; Evaluation of
wrinkle, texture, pore and inflammation of skin

10
Effect for skin
“Long-Lasting Moisturizing effect” for skin

Amitose R provides moisture and hydration to the skin and exhibits

skin conditioning efficacy.
Measurement of water content in the epidermal stratum corneum (in vivo test)

20
# p<0.1 (t-test) n = 13 High Skin conditioning effect
Amitose R
Suppression of multilayer
Capacitance()

15
Control
desquamation of stratum

Moisture
corneum
10
Anti-wrinkle effect
#
5 Suppression of rough skin

Low Skin pore tightening effect

0
0 3 6
Suppression of inflammation
Time (weeks)
[Method] A gel containing 1.0% of Amitose R was applied on the cheek of a volunteer (20’s to 40’s) twice a day (morning/ afternoon) for
6 weeks. The water content in the epidermal stratum corneum was measured with Corneometer CM-825 (Courage+ Khazaka
electronic GmbH) before use, after 3 weeks use and after 6 weeks use.

11
Effect for skin
Skin conditioning effect by moisturizing

Amitose R suppresses abnormal cornification and multilayer

desquamation of the stratum corneum by the skin moisturizing effect.
Evaluation of suppressing effect of multilayer desquamation of the stratum corneum
(in vivo test)

Before After 6 weeks

“Multilayer desquamation of
the stratum corneum” is …
A phenomenon that stratum corneum is
peeled off in folds through the tape-
stripping process. It is one of the index
standard of skin problems.

Desquamation degree: 38% Desquamation degree: 24%

[Method] A gel containing 1.0% Amitose R was applied on the cheek of a volunteer (20’s to 40’s) twice a day (morning/ afternoon) for 6 weeks. Then,
the stratum corneum (SC) on the cheek was collected by tape-stripping. SC transferred onto a glass slide was stained with 0.5% brilliant
green and 1.0% gentian violet for 10 min. After washing with water, the ratio of multilayer desquamation was analyzed for images observed
with an optical microscope (BX-51-FL, Olympus) . Degree of multilayer desquamation was calculated by the ratio of area in multilayer
desquamation and area in the total SC using the SC measuring program; Image-Pro Plus (Media Cybernetics Inc.)
[Conditions] Temperature: 20±2 deg C, humidity 40-50%

12
Effect for skin
Skin conditioning effect by moisturizing

Amitose R has anti-wrinkle effect and improves skin texture

by the skin conditioning effect resulting from moisturizing.

Wrinkle Texture

Before After 6 weeks Before After 6 weeks

Score: 36.2 Score: 12.4 Score: 26.5 Score: 13.0

[Method] A gel containing 1.0% Amitose R was applied on the cheek of a volunteer (20’s to 40’s) twice a day (morning/ afternoon) for 6 weeks. Wrinkle
score and Texture score were measured by VISIA evolution® (Canfield Scientific Inc.). The green marks in the left figure indicate scanned
wrinkle part. The yellow marks in the right figure indicate scanned cristae cutis and the blue marks indicate scanned sulci cutis of skin.
- Wrinkle score : Calculated value by both the number and the length of wrinkle.
- Texture score : Calculated value by cristae cutis and sulci cutis of skin.
[Conditions] Temperature: 20±2 deg C, humidity 40-50%

13
Effect for skin
Skin conditioning effect by moisturizing

Amitose R reduces skin pore and suppresses inflammation

by the skin conditioning effect resulting from moisturizing.

Pore Inflammation

Before After 6 weeks Before After 6 weeks

Score: 26.5 Score: 20.6 Score: 37.5 Score: 31.3

[Method] A gel containing 1.0% Amitose R was applied on the cheek of a volunteer (20’s to 40’s) twice a day (morning/ afternoon) for 6 weeks. Pore
score and red area’s score(result from inflammation) were measured by VISIA evolution® (Canfield Scientific Inc.). The violet marks in the left
figure indicate scanned pore part. The sky blue marks in the right figure indicate scanned skin redness.
- Pore score : Calculated value by both the number and the area of pores.
- Red area score : Calculated value by the state of inflammation, capillary vessel and acne .
[Conditions] Temperature: 20±2 deg C, humidity 40-50%

14
Effect for skin
Skin conditioning effect
in vivo test
(W/W%)

Volunteers Cream Control Amitose R

Amitose R - 3.0
13 volunteers (20’s to 50’s) PEG-45 Stearate 1.0 1.0
Glyceryl Stearate 3.0 3.0
Observation/evaluation Hydrogenated Jojoba Oil 2.0 2.0
(before use) Cetyl Alcohol 3.5 3.5
Stearic Acid 2.5 2.5
Squalane 7.0 7.0
Petrolatum 2.0 2.0
Application for 4 weeks Dimethicone 1.0 1.0

Volunteers applied formulae twice a day on their cheeks. Phenoxyethanol 1.0 1.0
Water Up to 100 Up to 100
Observation/evaluation
(after use)

Test-1; Measurement of water content

in the epidermal stratum corneum

Test-2; Evaluation of skin texture

15
Effect for skin
Skin conditioning effect

Amitose R gives moisture to dry skin and improves the skin

condition when formulated in cosmetics.
Measurement of water content in the Observation with microscope
epidermal stratum corneum (in vivo test) (in vivo test)
180
Before After 4 weeks
170
Amitose R High
Increase in capacitance(%)

Control

Amitose R
160

150

Moisture
140

130

Control
120

110
Low
100
0 1 2 3 4
Time (week)
[Method] Cream containing 3.0% of Amitose R was applied on the forearm of volunteers (20’s to 50’s) twice a day (morning/afternoon) for 4
weeks. Then water content in the epidermal stratum corneum is measured by using Corneometer CM-825 (Courage+
Khazaka electronic GmbH), and skin condition is observed by using USB microscope (Scalar Corporation).
[Conditions] Temperature: 20±2 deg C, humidity 40-50%

16
Effect for skin
Face wash

Amitose R keeps adsorbing to skin even after rinse-off and

moisturizing effect continues for a long time.
Sensory evaluation [Method]

Volume of foam 10 panelists evaluated the sensory-texture of skin

and gave 2 point to a better evaluation item, or 1
20 point to each items when they feel same sensory
texture. The graph shows the sum of total points.
15
Preference Texture of foam Reference formula No.0163 (W/W%)
10 (Fineness)
Face wash Control Amitose R
5
Amitose R － 1.0
0 Myristic Acid 7.0 7.0
Palmitic Acid 10.0 10.0
Stearic Acid 10.0 10.0
Smoothness Non-friction Lauric Acid 2.0 2.0
(after dry) (at rinse off) Butylene Glycol 10.0 10.0
Glycerin 3.0 3.0
PEG-150 Stearate 2.0 2.0
Moist-feel Cocamide Methyl MEA 2.0 2.0
Phenoxyethanol (90%),
(after dry) Ethylhexylglycerin (10%)
0.5 0.5
Potassium Hydroxide 5.3 5.3
Amitose R Control Water Up to 100 Up to 100

17
Effect for skin
Facial cleansing gel

Amitose R gives moist-feel both during use and after dry

thanks to closely adsorption to skin.
Sensory evaluation [Method]
8 panelists evaluated the sensory-texture of skin and
Moist feel gave 2 point to a better evaluation item, or 1 point to
(while in use) each items when they feel same sensory texture.
15 The graph shows the sum of total points.

10
Reference formula No.0142 (W/W%)
Smoothness 5 Moist-feel
(after rinse off) Face cleansing gel Control Amitose R
0 Amitose R - 1.0
Promois EFLS * 5.0 5.0
Ethylhexylglycerin 0.5 0.5
Glycerin 18.0 18.0
PEG-20 Glyceryl Triisostearate 5.0 5.0
Non-dryness Moist-feel
Caprylic/Capric Triglyceride Up to 100 Up to 100
(after dry) (after dry)
Water 1.0 －
* Sodium Lauroyl Hydrolyzed Silk, Water (SEIWA KASEI)
Amitose R Control

18
Effect for skin
Skin toner

Amitose R exhibits skin conditioning effect

due to uniform adsorption.
Sensory evaluation [Method]
11 panelists evaluated the sensory-texture
Affinity of skin and gave 2 point to a better
20 evaluation item, or 1 point to each items
when they feel same sensory texture.
15 The graph shows the sum of total points.
Preference Non-friction
10

5 Reference formula No.0162 (W/W%)

0
Skin ltoner Control Amitose R
Amitose R - 1.0
Glycerin 8.0 8.0
Smoothness Moist feel Butylene Glycol 4.0 4.0
Alcohol 4.0 4.0
Citric acid / Sodium Citrate * q.s. q.s.
Non-stickiness Phenoxyethanol 0.5 0.5
Water Up to 100 Up to 100
Amitose R Control * Adjusted to pH 5.5

19
Effect for hair

Validation of hair conditioning effect

20
Effect for hair
“Long-Lasting Moisturizing effect” for hair

Amitose R closely adsorbs to hair even after washing off

and exerts “Long-Lasting Moisturizing Effect”.
Confirmation of adsorption to hair

Bleached hair
SEM image TOF-SIMS image

Treatment
Hair was soaked in 1.0% water solution of Amitose R
and shaken at 40 deg C for 10 min.
 After that, it was washed with 40 deg C running water
and dried with towels and a drier.

Measurement of adsorption
to hair
Amitose R
[Measurement instrument]
TOF-SIMS (Time-of-Flight Secondary Ion Mass Spectrometry)

21
Effect for hair
“Long-Lasting Moisturizing effect” for hair

Amitose R closely adsorbs to hair even after washing off

and exerts “Long-Lasting Moisturizing Effect”.
Measurement of adsorption amount to hair

16.0
Bleached hair ** p<0.01 (t-test)

14.0
n=3 **

Adsorption amount to hair

12.0
Treatment
10.0
Hair was soaked in 1.0% water solution of Amitose R

(mg/g)
and shaken at 40 deg C for 10 min. 8.0
 After that, it was washed with deionized water.
Comparison; same treatment was conducted using
6.0
Glycerin instead of Amitose R.

4.0
Measurement of adsorption
amount to hair 2.0

[Measurement instrument] 0
LC-MS Glycerin Amitose R
LCMS-2020 (Shimadzu Co., Ltd.)

22
Effect for hair
Moisturizing effect

Measurement of secondary transpiration [Method]

moisture Moisturizing effect was evaluated by measurement of moisture
inside of the hair. The sample hair was heated at 65 deg C
(assumed the temperature of hair-dryer) for 40 min, and then the
Bleached hair (Asian) hair was heated at 180 deg C (assumed the temperature when
whole hair moisture transpires) for 30min. As shown in Figure,
based on the difference of weights between A and B,the
secondary transpiration moisture was calculated according to the
below equation.
Treatment (10 times)
65 deg C 180 deg C
Hair tress was soaked in 3% of active
solution with agitation at 40 deg C for 10 min.
Control was soaked in deionized water.
After that, they were washed with rapping Primary

Relative weight (%)

transpiration
water and dried with towels and a drier. moisture Moisture
in hair
A
Measurement of secondary Secondary
transpiration
transpiration moisture B moisture

[Measurement instrument]
Electric moisture balance 0 20 40 60 80
EB-340MOC (Shimadzu Co., Ltd.) Heating Time (min.)

Secondary transpiration moisture (A－B)

= ×100
(% dry base) B

[Reference] Seiji Nagao, Sakiko Uchida, Fragrance Journal, special issue, No.17, 169-174 (2000)

23
Effect for hair
Moisturizing effect

Amitose R closely adsorbs and gives moisture to damaged hair.

Measurement of secondary transpiration moisture

8.0
Secondary transpiration moisture

** p<0.01 (t-test)
n=5
High
7.0
**
6.0
(% dry base)

5.0

Moisture
4.0

3.0

2.0
1.0
Low
0
Control Amitose R

24
Effect for hair
Improvement of combing character and suppression of static electricity
[Evaluation method] (W/W%)

Shampoo Control Amitose R

Bleached hair (Asian)
Amitose R - 1.0 or 3.0
Sodium Laureth Sulfate(25%),
30.0 30.0
Water
Averaging combing load Cocamidopropyl Bethaine(30%),
10.0 10.0
Water
The tresses were divided into 3 tresses that gave the Cocamide DEA 3.0 3.0
same mean load value. Polyquaternium-10 0.2 0.2
Preservatives 0.5 0.5
Shampoo and conditioner (1 time for each) Citric Acid 0.1 0.1
Water Up to 100 Up to 100
Shampoo  Washing (in running water) 
conditioner  Washing (in running water)  Drying up conditioner Control Amitose R
* As the control, hair tresses treated with the control Amitose R - 1.0 or 3.0
shampoo and conditioner were used.
Behentrimonium Chloride(80%),
1.0 1.0
Isopropyl Alcohol(20%)
Combing and Electrostatic Cetearyl Alcohol 3.0 3.0
measurement Cetearyl Alcohol, Cetearyl
1.0 1.0
Glucoside
[Measurement instrument] Steareth-20 0.5 0.5
Combing tester (Techno Hashimoto) Glycerin 2.0 2.0
[Conditions]
Jojoba Seed Oil 2.0 2.0
Temperature: 23 deg C
Humidity (RH): 52% Preservatives 0.3 0.3
Water Up to 100 Up to 100

25
Effect for hair
Improvement of combing character and suppression of static electricity

Amitose R improves combing character

and suppresses static electricity of damaged hair.

Combing character Static electricity

7.0 8.0 Large

Low
Average value of loading (N/cm)

* p<0.05 (t-test) n=3

6.0 n=3
* 7.0

Combing performance
6.0

Static electricity
5.0

Static charge (kV)

5.0
4.0
4.0
3.0
3.0
2.0 2.0
1.0 1.0
High Small
0 0
Control
Amitose R Amitose R Control
Amitose R Amitose R
1.0% 3.0% 1.0% 3.0%

26
Effect for hair
Anti-hair frizzing effect

Amitose R suppresses hair frizzing in high humidity conditions.

Observation of hair style upkeep
Start After 6 hours After 24hours Prevents
humidity-induced
volume-up

Control Amitose R Control Amitose R Control Amitose R

[Method] On the right part of the wig of 10g of solution of 3.3% of Amitose R was applied. On the left side 10g of ion exchanged water
applied. The wig was set and hair style upkeep was observed.
[Conditions] Temperature: 26-28 deg C, Humidity 75±5 %

27
Effect for hair
Anti-dust / Anti-pollution effect
Evaluation of static particles adsorption on the hair (W/W%)
Shampoo Control Amitose R
Bleached hair Amitose R - 3.3
Sodium Laureth Sulfate (25%),
30.0 30.0
water
Cocamidopropyl Betaine (30%),
Shampoo and conditioner (1 time for each) water
10.0 10.0

Cocamide DEA 3.0 3.0

Shampoo  Washing (in running water) 
Polyquaternium-10 0.2 0.2
conditioner  Washing (in running water)  Drying up
* As the control, hair tresses treated with the control Seisept H* 0.5 0.5
shampoo and conditioner were used. Citric Acid 0.1 0.1
water Up to 100 Up to 100
Drying
Conditioner Control Amitose R
Hair tresses were left 15 hours at Amitose R - 3.3
Low temperature (RH 30~40%) . Behentrimonium Chloride (80%),
1.0 1.0
Isopropyl Alcohol (20%)
Application of microbeads Cetearyl Alcohol 3.0 3.0
Cetearyl Glucoside, Cetearyl
1.0 1.0
Alcohol
Hair tresses were immersed 1min in charged
microbeads. Steareth-20 0.5 0.5
Glycerin 2.0 2.0
Simmondsia Chinensis (Jojoba)
Evaluation an observation of Seed Oil
2.0 2.0
hair tresses Seisept H*1 0.3 0.3
Water Up to 100 Up to 100
*Ethoxydiglycol, Methylparaben, Phenoxyethanol, Propylparaben, Butylparaben,
Ethylparaben (SEIWA KASEI)

28
Effect for hair
Anti-dust / Anti-pollution effect

Amitose R prevents the adsorption of charged particles

in low humidity conditions

Quantity of adsorbed particles

n=3
0.2
Few particles Dirty

Quantity of microbeads (g)

adsorbed 0.16

0.12

0.08

0.04
Clean
0
Control Amitose R
Control Amitose R

29
Effect for scalp

Validation of scalp care effect

30
Effect for scalp
Scalp care effect
in vivo test (W/W%)
Shampoo Control Amitose R
Volunteers Amitose R - 1.0
Promois EFLS-F *1 37.5 37.5
22 volunteers; male and female in 20’s to 60’s Sodium Laureth Sulfate (25%), Water 8.0 8.0
PEG-120 Methyl Glucose Dioleate 1.0 1.0
Observation and evaluation of scalp Citric Acid/ Sodium Citrate 0.5 0.5
Polyquaternium-10 0.3 0.3
Methylparaben 0.2 0.2
Water Up to 100 Up to 100
Continuous use of
shampoo and conditioner conditioner Common
(for 4 weeks) Steartrimonium Chloride (28%), Water 4.5
Behentrimonium Chloride (80%), Isopropyl Alcohol (20%) 1.2
Once a day Glyceryl Stearate 1.2
Cetyl Alcohol 4.8
Observation and evaluation of scalp Paraffin 1.8

Observation of scalp; evaluation of dandruff Octyldodecanol 0.6

Isopropyl Myristate 1.2
VAS questionnaire: evaluation of scalp itching Propylparaben 0.1
Methylparaben 0.1
[Measurement instrument]
Digital microscopy KH-3000 (HIROX Co., Ltd.) Dimethicone 3.0
Dimethicone(500 cs) 1.0
Water Up to 100
*1 Sodium Lauroyl Hydrolyzed Silk, Water (SEIWA KASEI)

31
Effect for scalp
Dandruff prevention effect

Amitose R suppresses the dryness of scalp by hair washing

and prevents dandruff when formulated in shampoo.
Observation of scalp

Before use After 4 weeks

Dandruff!!

32
Effect for scalp
Scalp itching improving effect

Amitose R relieves scalp itching.

VAS questionnaire about scalp itching (in vivo continuous use test)

30
* p<0.10 (t-test)
20 High What’s VAS (Visual Analogue Scale)
10
questionnaire ?

Scalp itching
-1.7 Method used to evaluate numerically sensations such as itching:
0
VAS Score

intensity
The VAS is a 10-cm long line, on which subjects indicate the itch intensity by
marking the line at the point that corresponds to the degree of their itch, where
-10 the beginning of the scale refers to no itch (0 point) and the end of the scale to
*
the worst imaginable itch(10 points). It provides numerical values of the itch
-20 intensity from 0 (no itch) to 10 (the worst imaginable itch), and each subjects
-21.8 must indicate a number that corresponds to the perceived itch intensity.
-30

-40 Low
100 mm
Worst
No itch imaginable
-50 XX mm
0 100 itch
Before After
Answer
Amitose R Control

33
Effect for skin microbiome

Validation of skin microbiome improvement effect

34
What is the skin microbiome ?

“Skin Microbiome” refers to the community of bacteria

which inhabit on the skin and interacts with skin condition.

The microbiome of a healthy skin The microbiome of a unhealthy skin

- Good bacteria predominant - Bad bacteria predominant

- Very diverse bacteria - Unbalanced bacteria composition

35
What is the skin microbiome ?
Common bacteria of the skin microbiome

Good bacteria Opportunistic Bad bacteria

bacteria
Staphylococcus epidermidis Propionibacterium acnes Staphylococcus aureus

Called the beautiful skin bacteria, they are
found in number on healthy skins. They
keep the skin slightly acid and produces
anti-bacterial substances which prevent
the proliferation of S.aureus.
Called the bacteria of acne, they help the
skin to keep its good condition.
Nevertheless, when they grow too much
the cause inflammations and induce acne.
Known as the bacteria of roughness, they
are found in number in rough skins.
When some skin troubles makes the skin
more alkaline, they multiply and worsen
the troubles and leading to inflammation.

There are more than 1000 species of bacteria on the skin.

The composition of the skin microbiome depends on individuals and changes daily.

36
Effect for skin microbiome
Bacteria proliferation & utilization
“Utilization” is a biological phenomenon where a given substance
is used as a nutrient by bacteria which as a consequence proliferate.

[Non utilization] [Utilization]

Bacteria

Bacteria

Substance Substance

The substance in not utilized. The substance is utilized.

Bacteria do not proliferate Bacteria proliferate

37
Effect for skin microbiome
Utilization by S.epidermidis (good bacteria)

Amitose R is utilized by S.epidermidis which multiplies and

improves skin beauty.
Measurement of S.epidermidis increase Observation of culture plates
n=3 **p<0.01, *p<0.05 (t-test) significant from control Control Glycerin
0.14 High
0H 24H 48H

0.12

Increase in S.epidermidis
OD 620 variation rate

0.10 S.epidermidis
** increased ! **
0.08
*
0.06 *
0.04
Amitose R
0.02

0 Low

[Method] BPW medium with 1.0% sample was mixed with Staphylococcus epidermidis and cultured at 37 deg C for 48 hours. At each
stage(After mixing, 24 h after, 48 h after), OD620 was measured. The culture plates were observed after 48h.

38
Effect for skin microbiome
Utilization by S.aureus (bad bacteria)

Amitose R is not utilized by S.aureus so no skin trouble is to be

expected.
Measurement of S.aureus increase Observation of culture plates
n=3 **p<0.01, *p<0.05 (t-test) significant from control Control PCA-Na
0.14 High
0H 24H 48H

0.12
OD 620 variation rate

**

Increase in S.aureus
0.10
No change in
0.08 S.aureus **
**
0.06 * **
0.04 *
Amitose R
0.02

0 Low

[Method] BPW medium with 1.0% sample was mixed with Staphylococcus aureus and cultured at 37 deg C for 48 hours. At each stage(After
mixing, 24h after, 48 h after), OD620 was measured. The culture plates were observed after 48h.

39
Effect for skin microbiome
Utilization by P.acnes (opportunistic bacteria)

Amitose R is not utilized by P.acnes, so risks of acne are

minimized.
Measurement of P.acnes increase Observation of culture plates
n=3 **p<0.01, *p<0.05 (t-test) significant from control Control Glycerin
1.20 High
0H 24H 48H

1.00 **
OD 620 variation rate

**

Increase in P.acne
0.80
No change in
0.60 P.acne **

0.40 **
** * * Amitose R
0.20

0 Low

[Method] GAM semisolid medium with 1.0% sample was mixed with Propionibacterium acnes and cultured at 37 deg C for 48 hours under
anaerobic conditions. At each stage(After mixing, 24 h after, 48 h after), OD620 was measured. The culture plates were
observed after 48h.

40
Effect for skin microbiome
Utilization by P.acnes (opportunistic bacteria)

Amitose R isn’t utilized by P.acnes, so risks of acne are minimized.

Utilized moisturizers
ex. Glycerin
Sebum barrier Epidermis Proliferation

P.acnes
Washing Moisturizer
Cleansing having utilization

Moisturizer was used as nutrient of P.acnes

Without care Sebaceous gland

No effect Amitose R
Proliferation Amitose R

Excess sebum
Amitose R isn’t
propagates P.acnes utilized by P.acnes

41
Product Information

Glycerin Derivative of Arginine

Composition

INCI name Chinese INCI name CAS No. Composition

Dihydroxypropyl Arginine HCl 二羟丙基精氨酸 HCl 787607-61-8 30%

Water 水 7732-18-5 70%

* This product contains 1.0% of Phenoxyethanol as an additive.

We can provide preservative-free type product, Amitose R-PD, containing 1.0% of Pentylene Glycol as an additive.

All rights reserved. Data appear on this catalog are actual obtained values by our experimental procedures, but not the specification values. Upon the use of the material on this catalog, please
verify the functions, effects and safety of the materials in your company’s using conditions. SEIWA KASEI cannot guarantee that the applications, which are introduced in this catalog, never
interfere with any patents. For certain reasons, the information of this catalog may be changed without notice.

1-2-14, Nunoichi-cho, Higashiosaka, Osaka, Japan URL: https://www.seiwakasei.co.jp/en

TEL +81-72-987-2626 FAX +81-72-987-2072 e-mail: sales@seiwakasei.co.jp 42