Matrix effect determination for methiocarb-sulphoxide in tomato

post extraction spiked sample (0.4 mg/Kg)

c (mg/Kg) %𝑀𝐸= 〖𝐴𝑛𝑎𝑙𝑦𝑡𝑒 𝑠𝑖𝑔𝑛𝑎𝑙〗 _(𝑝𝑜𝑠𝑡−𝑒𝑥𝑡𝑟𝑎𝑐𝑡𝑖𝑜𝑛 𝑠𝑝𝑖𝑘𝑒𝑑 𝑚

Signal
0.4 2.45E+07
0.4 2.56E+07
0.4 2.63E+07

average signal 2.55E+07

%ME= 28.0%

Calibration graph in solvent

c (mg/Kg) Signal
0.01 2.52E+06
0.04 8.40E+06
0.1 3.53E+07
0.4 9.08E+07
1 2.93E+08
4 1.08E+09

100% no supresion no mejora

>100% mejora
<100% supresion
n tomato

𝑡𝑟𝑎𝑐𝑡𝑖𝑜𝑛 𝑠𝑝𝑖𝑘𝑒𝑑 𝑚𝑎𝑡𝑟𝑖𝑥)/ 〖𝐴𝑛𝑎𝑙𝑦𝑡𝑒 𝑠𝑖𝑔𝑛𝑎𝑙〗 _𝑠𝑜𝑙𝑣𝑒𝑛𝑡 ∗100%

Matrix effect determination for methiocarb-sulphoxide in tomato

Post-extraction spiked sample (0.4 mg/kg)

Cspiked (mg/Kg) Signal Ccalculated (mg/Kg)

0.4
%𝑀𝐸= 〖𝐴𝑛𝑎𝑙𝑦𝑡𝑒 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 𝑓𝑜𝑢𝑛𝑑〗 _(𝑝𝑜𝑠𝑡−𝑒𝑥𝑡𝑟𝑎𝑐
2.45E+07 0.090
0.4 2.56E+07 0.094
0.4 2.63E+07 0.097

Average concentration= 0.094 mg/Kg

%ME = 23.5%

Calibration graph in solvent

c (mg/Kg) Signal
0.01 2.52E+06 slope 2.71E+08 0.00E+00 intercept
0.04 8.40E+06 3.21E+06 #N/A
0.1 3.53E+07 9.99E-01 1.33E+07
0.4 9.08E+07
1 2.93E+08
4 1.08E+09
 tomato

〗 _(𝑝𝑜𝑠𝑡−𝑒𝑥𝑡𝑟𝑎𝑐𝑡𝑖𝑜𝑛 𝑠𝑝𝑖𝑘𝑒𝑑 𝑚𝑎𝑡𝑟𝑖𝑥)/ 〖𝑆𝑝𝑖𝑘𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛〗 _𝑠𝑜𝑙𝑣𝑒𝑛𝑡 ∗100%

〗 _𝑠𝑜𝑙𝑣𝑒𝑛𝑡 ∗100%
Aldicarb in tomatos
c(mg/Kg) Signal Solvent Signal Matrix
0.04 1.14E+07 1.75E+07
0.1 3.28E+07 4.87E+07
0.4 1.62E+08
%𝑀𝐸= 〖𝐴𝑛𝑎𝑙𝑦𝑡𝑒 𝑠𝑖𝑔𝑛𝑎𝑙〗 _(𝑝𝑜𝑠𝑡−𝑒𝑥𝑡𝑟𝑎𝑐𝑡𝑖𝑜𝑛
1.84E+08
1 2.82E+08 3.46E+08
4 1.18E+09 1.46E+09

1.60E+09
Matrix calibration function
1.40E+09
slope 3.65E+08 0.00E+00 intercept 1.20E+09
5.34E+06 #N/A
1.00E+09
9.99E-01 2.21E+07
8.00E+08

señal
6.00E+08
Calibration function in solvent
4.00E+08

slope 2.95E+08 0.00E+00 intercept 2.00E+08

5.55E+06 #N/A
0.00E+00
9.99E-01 2.30E+07 0 0.5

%ME= 123.56%

*mejora ionizacion
correlating compound taht has exactly the same ms/ms transition como aldicarb

correlating matrix compound that increases our ionization efficiency of our compound

pequeñas variaciones en la matriz pueden cambiar el efecto matriz

para tener unn metodo robusto necesitamos tener un efecto matriz cercano a 100%
(𝑝𝑜𝑠𝑡−𝑒𝑥𝑡𝑟𝑎𝑐𝑡𝑖𝑜𝑛 𝑠𝑝𝑖𝑘𝑒𝑑 𝑚𝑎𝑡𝑟𝑖𝑥)/ 〖𝐴𝑛𝑎𝑙𝑦𝑡𝑒 𝑠𝑖𝑔𝑛𝑎𝑙〗 _𝑠𝑜𝑙𝑣𝑒𝑛𝑡 ∗100%

1.60E+09

1.40E+09

1.20E+09
solvente
1.00E+09

8.00E+08 Linear
señal

(solvente)
6.00E+08
matrix
4.00E+08

2.00E+08 Linear (ma-

trix)
0.00E+00
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5
c mg/Kg
s transition como aldicarb

tion efficiency of our compound

efecto matriz

efecto matriz cercano a 100%

𝑛𝑡 ∗100%