Cytotoxic and genotoxic effects of lead and cadmium on HL-60 and Jurkat cell lines Pon.9 A. Soft *!, E, Dawovié "1M. Shivié Huseinovie*, M. Bengunt", D, Subtovel, N. Sabovié A. Smalovis' L. Begs! "University of Tua, Faculty of Pharmacy, Tura, Bosnia and Herregvin,"Univeity of Nova Goria, Laborsory for Enviroamentl an Lif Scienes, Nova Gora, Slovenia, Unvenity Cina Comer Tula, Tl, Bosnia an Herzegovina HI-60 cella Jurkat cells were used as moves to explore the single and cambined cytotoxic effet of ea (Pb) and cadmium (Ca) on immune cells Ak, the voto potential of each individ metal was analyzed. Lead (10-100 jM) and cadmium (25-10 uM) were introduced individually and as mines cel ies fr 24 ‘hours. The concenrations of Pb and Ca forthe analysis of mixture intracton were scl from their individual cyotaxic effets. The eet adetivity metod was utilize, in which concentration ofeach compound, equivalent is 1C20, was calulate from single metal response curve. Forte assesment of eytetucity cells were incubated for hours with Peso lve reagent and the shsorhance was measured at $70 rn, Statistical analysis was performed according o Stadeets lest p ‘values below 0.08 wer considered significant. To determine genotoxicity of Ph and Cd indirect immunoftutescence mcd with primary ant-pespho-hysone HZA.X antibody was used, The genotoxic efccs were evaluated by the csquare tet p values below 0.05 were considered significant. Lead and cadmium caused ‘etotoxie effects bo cx nes Cadmium exhibited higher eytotaxc potential. The most pronounced cytotoxic effect was observed atte lowest concentations of metals (25 pM fora and 25M for Pb, Funhernccase in metal concentaion did nt Kd to propetonal decline in el viability, in both ines, respectively. Metal mixtures showed synergistic efet in IL-60 cells and antagonistic eet in Just cls, compare to individual metas. Cadmium exhibited more potent genotoxic effet than ead, and showed dose dependence in bath cll ines. The combined ets should be considered inthe sk assexsment of heavy metal onc. Previously published in: Dautovi Eet al (2019) Gents & Applications 3, 57-64 Lau K, MeLean WG, Willams DP, Howard CV. (2006) Toxicol Se 90,175-187. The authors marked with an asters equsly contributed tthe work