College of Medical Laboratory Science | OUR LADY OF FATIMA UNIVERSITY

Name: _________________________________________________ Date: __________________

Section: __________________ Group: __________________ Score: _________________

The student will have to research and answer the different procedures in the experiment worksheet.
Provide rationale behind the results of each procedure.

The work area of the experiment should be sterilized. The student should wear proper personal
protective equipment before the experiment (laboratory gown, eye protector, hair net, laboratory mask, and
gloves). The student should be familiar to the chemicals and laboratory procedures involve in every
laboratory experiments so that proper handling will be observed. First aid treatment and safety facilities
should be in noted by the faculty before the start of any procedure.

Attentiveness is NECESSARY. DO NOT play with the chemicals! Follow the procedure as
instructed and follow the proper disposal of the chemicals.

*Note that this phase is not applicable since we are having laboratory classes virtually.

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College of Medical Laboratory Science | OUR LADY OF FATIMA UNIVERSITY
0.5% Bromine in Carbon tetrachloride 2% potassium permanganate Benzene
10% Sodium hydroxide Concentrated Sulfuric acid Hexane
Nitrating mixture: 1mL of concentrated nitric acidand 1 mL of concentrated sulfuric acid
Test tube Light match Watch glass

A. Physical State, Color and Odor

1. Observe the physical state of the benzene and hexane at room temperature.
2. Note the color and odor with a waffling motion of hand and investigate the odor.
Characteristics Observations
Benzene Hexane
Physical state Colorless Liquid Colorless Liquid
Color Light Yellow Clear
Odor Sweet / Rugby-like Gas-like / Petroleum-like / Balloon-like

B. Solubility in concentrated sulfuric acid

Tube 1 – Sample Tube 2 – Sample Tube 3 – Control
5 drops of benzene 5 drops of hexane 5 drops of distilled water
10 drops of concentrated 10 drops of concentrated 10 drops of concentrated
sulfuric acid sulfuric acid sulfuric acid
Observe for color and warming effect.
Observation: Observation: Observation:
Formed Translucent Oily Layer and Formed Oily Layer, the Most Clear in Color and the Warmest
second to the warmest Yellowish and No Warming Effect
C. Ignition test
Watch glass 1 – Sample Watch glass 2 – Sample
5 drops of benzene 5 drops of hexane
Light with a match Light with a match
Note the color of the flame
Observation: Observation: Orange Flame and Non-soot
Red-orange Flame, W/ Soot, and Emits Black Smoke
D. Test for active unsaturation

a. Baeyer’s test
Tube 1 – Sample Tube 2 – Sample Tube 3 – Control
5 drops of benzene 5 drops of hexane 5 drops of distilled water
2 drops of 2% KMnO4 2 drops of 2% KMnO4 2 drops of 2% KMnO4
Shake and note the formation of brown suspension.
Observe the rate and extent of decolorization
Observation: Observation: Observation:
Brown Suspension is Visible Brown Suspension is Visible / Brown Suspension is Not Visible
Dark Magenta

b. Bromine test
Tube 1 – Sample
5 drops of benzene
10 drops of 0.5% Bromine
in Carbon tetrachloride
Tube 2 – Sample Tube 3 – Control
5 drops of hexane 5 drops of distilled water
10 drops of 0.5% Bromine 10 drops of 0.5% Bromine
in Carbon tetrachloride in Carbon tetrachloride

CHEM111 LABORATORY MANUAL | 2

 College of Medical Laboratory Science | OUR LADY OF FATIMA UNIVERSITY
Observe the rate and extend of decolorization
Observation: Yellowish Observation: Yellowish Observation: Clear / Colorless

c. Test for aromaticity

Tube 1 – Sample Tube 2 – Sample Tube 3 – Control
5 drops of benzene 5 drops of hexane 5 drops of distilled water
8 drops of nitrating mixture 8 drops of nitrating mixture 8 drops of nitrating mixture
Shake.
Note the color formation of oily layer. If there is no reaction within 1 minutes, place it in
water bath at 50°c for 10 minutes.
Observation: Has Oily Layer Observation: Has Oilier Layer Observation:
Non-Oily Layer / Colorless
d. Basic oxidation test
Tube 1 – Sample Tube 2 – Sample Tube 3 – Control
5 drops of benzene 5 drops of hexane 5 drops of distilled water
8 drops 2% KMnO4 8 drops 2% KMnO4 8 drops 2% KMnO4
3 drops of 10% NaOH 3 drops of 10% NaOH 3 drops of 10% NaOH
Water bath both tubes for 10 minutes
Note the color change and formation of precipitate.
Observation: Violet Observation: Blue-green Observation: Violet

Before leaving, the students must do all of the following:

o Returning of materials, chemicals and equipment
o Disposal of wastes materials.
o Disinfection of the working area
o The PPE of each individual should be removed properly. These cannot be exposed outside the
laboratory premises.
o Disposal of chemicals reaction residues in the experiment in their proper container or as instructed
by the faculty
*Note that this phase is not applicable since we are having laboratory classes virtually.

Outcome To achieve each outcome, the learner must

demonstrate the ability to:
Research and record the appropriate products of a. Research and record the appropriate products
the different test reactions. of the different test reactions.

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