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    AUBF-EXP-6 - Angowan

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    Nimfa Gumiran
    The document summarizes key differences between hematuria, hemoglobinuria, and myoglobinuria based on parameters from a patient's urine and blood test results. It also provides details on the composition, sensitivity, potential interferences, and test correlations of blood reagent strips commonly used to detect these conditions. Specifically, it notes that hematuria involves red blood cells in urine sediments, while hemoglobinuria and myoglobinuria show elevated muscle enzyme levels but few or no red blood cells. The reagent strips contain compounds that produce a color change in the presence of blood, with varying sensitivity cut-offs, and can be subject to false positives or negatives from certain substances.

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    AUBF-EXP-6 - Angowan

    Uploaded by

    Nimfa Gumiran
    9 views3 pages
    The document summarizes key differences between hematuria, hemoglobinuria, and myoglobinuria based on parameters from a patient's urine and blood test results. It also provides details on the composition, sensitivity, potential interferences, and test correlations of blood reagent strips commonly used to detect these conditions. Specifically, it notes that hematuria involves red blood cells in urine sediments, while hemoglobinuria and myoglobinuria show elevated muscle enzyme levels but few or no red blood cells. The reagent strips contain compounds that produce a color change in the presence of blood, with varying sensitivity cut-offs, and can be subject to false positives or negatives from certain substances.

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    AUBF-EXP-6- Angowan

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    The document summarizes key differences between hematuria, hemoglobinuria, and myoglobinuria based on parameters from a patient's urine and blood test results. It also provides details on the composition, sensitivity, potential interferences, and test correlations of blood reagent strips commonly used to detect these conditions. Specifically, it notes that hematuria involves red blood cells in urine sediments, while hemoglobinuria and myoglobinuria show elevated muscle enzyme levels but few or no red blood cells. The reagent strips contain compounds that produce a color change in the presence of blood, with varying sensitivity cut-offs, and can be subject to false positives or negatives from certain substances.

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    © All Rights Reserved
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    9 views3 pages

    AUBF-EXP-6 - Angowan

    Uploaded by

    Nimfa Gumiran
    The document summarizes key differences between hematuria, hemoglobinuria, and myoglobinuria based on parameters from a patient's urine and blood test results. It also provides details on the composition, sensitivity, potential interferences, and test correlations of blood reagent strips commonly used to detect these conditions. Specifically, it notes that hematuria involves red blood cells in urine sediments, while hemoglobinuria and myoglobinuria show elevated muscle enzyme levels but few or no red blood cells. The reagent strips contain compounds that produce a color change in the presence of blood, with varying sensitivity cut-offs, and can be subject to false positives or negatives from certain substances.

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    of 3

    SAINT MARY’S UNIVERSITY

    School of Health and Natural Sciences


    DEPARTMENT OF MEDICAL LABORATORY SCIENCE

    Name: Angowan, Conie Rose Date: 11/09/22

    EXPERIMENT 6
    TEST DETERMINATION FOR BLOOD

    Drawings:
    Draw and label properly:
    1. Results of Benzidine test.

    Question for Research:


    1. In tabulated form, differentiate Hematuria, Hemoglobinuria, and
    Myoglobinuria using the following parameters;

    she mendoza-ramos,rmt AUBF Lab


    SAINT MARY’S UNIVERSITY
    School of Health and Natural Sciences
    DEPARTMENT OF MEDICAL LABORATORY SCIENCE

    Parameter Hematuria Hemoglobinuria Myoglobinuria

    Reagent strip for blood Positive Positive Positive


    reaction

    Urine sediments for RBC Present Absent(few) Absent(few)

    Urine appearance Cloudy red Cloudy red Cloudy red-brown

    Plasma appearance Normal Pink to Normal


    red(hemolysis)

    Total serum Normal Slight elevation(10 Marked


    creatinekinase (CK) times normal upper elevation(42 times
    limit) marked elevation)
    Total serum lactase Normal Elevated Elevated
    dehydrogenase (LDH)

    LDH1 ad LDH2 Normal Elevated Normal

    LDH4 ad LDH5 Normal Normal Elevated

    2. Give the following details about the blood reagent strip;


    a. Reagent composition
     Multistix: Diisopropylbenzene
     dihydroperoxide and
     3,3’,5,5’-tetramethylbenzidine
     Chemstrip: dimethyldihydroperoxy hexane
     tetramethylbenzidine

    b. Sensitivity
     Multistix: 5 to 20 RBCs/mL, 0.015 to

    she mendoza-ramos,rmt AUBF Lab


    SAINT MARY’S UNIVERSITY
    School of Health and Natural Sciences
    DEPARTMENT OF MEDICAL LABORATORY SCIENCE

    0.062 mg/dL hemoglobin


     Chemstrip: 5 RBCs/mL, hemoglobin
    corresponding to 10 RBCs/mL

    c. Interferences:
    False-positive:
     Strong oxidizing agents
     Bacterial peroxidases
     Menstrual contamination
    False-negative:
     High specific gravity/crenated cells
     Formalin
     Captopril
     High concentrations of nitrite
     Ascorbic acid greater than 25 mg/dL
     Unmixed specimens

    d. Test correlation:
     Protein
     Microscopic

    she mendoza-ramos,rmt AUBF Lab

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