NO ANIMAL DIED TO MAKE THIS STEAK

The first 3-D-printed ribeye relies on tissue-engineering technology

Stephen Ornes
May 21, 2021

It looks like a steak. It cooks like a steak. And according to the scientists who made and
ate it, the thick and juicy slab smells and tastes like a steak. A ribeye, specifically. But
appearances can be deceiving. Unlike any steak found on a menu or store shelf today, this one
didn’t come from a slaughtered animal.
Scientists printed it earlier this year with a bioprinter. The machine is much like a
standard 3-D printer. The difference: This type uses cells as a form of living ink.
“The technology involves the printing of actual living cells,” explains biologist Neta
Lavon. She helped develop the steak. Those cells are incubated, she says, to “grow in a lab.” By
that she means they’re given nutrients and kept at a temperature that lets them keep growing.
Using real cells this way, she says, is a real innovation over previous “new meat” products. This
allows the printed product “to acquire the texture and qualities of a real steak.”
Lavon works at Aleph Farms, a company in Haifa, Israel. Her team’s steak project grew
out of a partnership between the company and scientists at Technion–Israel Institute of
Technology, which is in Rehovot. The ribeye is the latest addition to a growing list of meats
grown in a lab instead of as part of some animal.
Researchers call these new meats “cultivated” or “cultured.” Interest in them has grown
in recent years, partly because the technology shows they are possible. Advocates say that if
meat can be printed, then no animal would need to lose its life to become human food. But
don’t look for these products on the store shelves just yet. Making meat this way is much harder
— and therefore costs more — than raising and killing an animal. “The technology will require
drastic reductions in cost before cultured meat will be widely available,” says Kate Krueger. She’s
a cell biologist in Cambridge, Mass., who started Helikon Consulting. Her business works with
companies who want to grow animal-based foods from cells.
One of the most expensive components, says Krueger, is the cell-growth medium. This
mix of nutrients keeps the cells alive and dividing. The medium contains expensive ingredients
called growth factors. Unless the cost of growth factors drops, says Krueger, “cultured meat
can’t be produced at comparable prices to animal meat.”
The ribeye joins a growing list of cultured meat products. It started in 2013. Back then,
a physician and scientist named Mark Post debuted the world’s first burger made with lab-
grown meat. Three years later, Memphis Meats, based in California, unveiled a cultured-meat
meatball. In 2017, it debuted cultured duck and chicken meat. Aleph Farms entered the picture
the next year with a thin-cut steak. Unlike its new ribeye, it was not 3-D-printed.
To date, none of these cultured-meat products is yet on sale in stores.
The companies working on them use technology borrowed from tissue engineering.
Scientists in this field study how to use real cells to build living tissues or organs that might help
people.
At Aleph Farms, the process of building a ribeye begins with collecting pluripotent stem
cells from a cow. Scientists then place these in a growth medium. This type of cell can produce
more cells by dividing again and again. They are special because they can develop into almost
any type of animal cell. For instance, Lavon notes, “They can mature into the cell types that
comprise meat, such as muscle.”
The incubated cells will grow and reproduce. When there are enough, a bioprinter will
use them as a “living ink” to build a printed steak. It lays the cells down one layer at a time. This
printer also creates a network of tiny channels “that mimic blood vessels,” Lavon says. These
channels let nutrients reach the living cells.
 After printing, the product goes in what the company calls a tissue bioreactor. Here, the
printed cells and channels grow to form a single system. The company hasn’t yet shared how
long it takes to print a ribeye from start to finish.
Lavon says the technology works, but can’t yet print lots of ribeye steaks. She predicts
that within two or three years, though, cultured ribeye steaks could reach supermarkets. The
company plans to start selling its first product, that thin-cut steak, next year.
Like Krueger, Lavon says costs remain a challenge. In 2018, Aleph Farms reported that
producing one serving of cultured steak cost $50. At that price, Lavon says, it can’t compete with
the real thing. But if scientists can find lower-cost methods, she says, then tissue engineering
may stand a chance of giving beef without the moo.

RETRIEVED FROM: https://www.sciencenewsforstudents.org/article/innovation-no-animal-

died-to-make-this-steak
ACCESS ON: JULY, 27TH 2021.