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L3 - EILab Manual - Sem5
L3 - EILab Manual - Sem5
Gandhinagar
Semester: - V
Gandhinagar
CERTIFICATE
Date:- 24-9-21
Institute
Vision:To be a premier engineering institution, imparting quality education for innovative
solutions relevant to society and environment.
Mission:
1. To develop human potential to its fullest extent so that intellectual and innovative
engineers can emerge in a wide range of professions.
2. To advance knowledge and educate students in engineering and other areas of
scholarship that will best serve the nation and the world in future.
3. To produce quality engineers, entrepreneurs and leaders to meet the present and
future needs of society as well as environment.
Department
Vision: To impart quality education in Instrumentation & Control engineering through
innovation to meet technological challenges of industries and society with environmental
consideration.
Mission:
1. To impart knowledge about present trends in the field of Instrumentation & Control
engineering.
2. To train competent Instrumentation & Control engineers who can design, operate process
instrumentation and also manufacturing an automation systems in industries.
3. To encourage students to work on innovative research projects related to
Instrumentation & Control engineering considering environmental aspect.
General Instructions
Do’s
➢ Ensure your presence five minutes before the commencement of the lab.
➢ Attend all the lab sessions without fail.
➢ All bags must be kept at the indicated place.
➢ Come well prepared for every lab session.
➢ Complete and Bring the Lab records regularly.
➢ Ensure the proper polarity of cables before connecting the kits.
➢ Ensure the checking of the circuit connections before turning ON the circuit.
➢ Tuck in your shirts and not to play with instruments laid on the bench.
➢ Wearing loose garments inside the lab is strictly prohibited.
➢ You have to wear shoes compulsorily.
➢ Keep the space around you clear for others.
Don’ts
➢ Don’t bring the food items to Lab.
➢ Don’t switch ON voltage supplies after making circuit connections in the absence
of the teacher.
➢ Don’t rotate the Knobs unnecessarily.
➢ Don’t make noise in the laboratory.
INDEX
Sr. No. Name of Experiments Pg. No. Date
High volatility
Thermal
stability
Low molecular weights
Mobile phase
Carrier gas – This is needed for the transfer of the injected sample to the separation
column. They are also responsible for the subsequent transfer of separated
components to the detector.Common examples: Nitrogen, helium, or hydrogen
Fuel gas – They support the flame in Flame ionization detector (FID) detector such as
Hydrogen.
Zero air – These are the purified air that plays the role of oxidant to support the
combustion of flame in the detector. Before being led to the gas chromatographic
system, the above three are intermixed in the desired proportion.
Sample injector
The injector is a heated block where the sample is injected. Through the carrier gas
stream, the sample is spontaneously vaporized and led to the column.
With the help of a gas-tight syringe, the liquid sample mixtures are injected whereas,
with the help of automated injection valves, the gaseous mixtures are injected.
Column
This is filled with the stationary phase or its walls are covered with a liquid adsorbent.
This is done for selective absorbance and retention of the sample components.
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Commonly used: Packed columns and Capillary columns (More
Detector
Here, the regions of individual peaks created relate to their concentrations and their
retention times are representative of their identity.
Conclusion:
In this experiment we learn that gas chromatography is one of the most important tools in
chemistry because of its simplicity , sensitivity and effectiveness in separating components of
mixtures. It is widely used for qualitative and quantitative analysis of mixture and for the
purification of compounds.
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EXPERIMENT-07
AIM: Study of High Performance Liquid Chromatography.
THEORY:
As bands emerge from the column, flow carries them to one or more detectors which
deliver a voltage response as a function of time. This is called a chromatogram. For
each peak, the time at which it emerges identifies the sample constituent with respect
to a standard. The peak’s area represents the quanti ty.
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Basic HPLC System Components:
Solvent Degasser – removes air gases from the solvents as they flow to the HPLC
pump
HPLC Pump –Pump is positioned in the most upper stream of the LC system and generates a flow of
eluent from the solvent reservoir to the system. In the earlier stage of LC development, to be able to
generate the high pressure was the one of the most important system requirements. However, nowadays,
the high pressure generation is a “standard” requirement and what is more concerned nowadays is to be
able to provide a consistent pressure at any condition, to provide a controllable and reproducible flow rate.
Since a change in the flow rate can influence the analysis largely.
Most pumps used in current LC systems generate the flow by back-and-forth motion of a motor-
driven piston (reciprocating pumps). Because of this piston motion, it produces “pulses”. There have been
large system improvements to reduce this pulsation and the recent pumps create much less pulse
compared to the older ones. However, recent analysis requires very high sensitivity to quantify a small
amount of analytes, and thus even a minor change in the flow rate can influence the analysis.
Injector-An injector is placed next to the pump. The simplest method is to use a syringe, and the
sample is introduced to the flow of eluent. Since the precision of LC measurement is largely affected by
the reproducibility of sample injection, the design of injector is an important factor. The most widely used
injection method is based on sampling loops. The use of autosampler (auto-injector) system is also widely
used that allows repeated injections in a set scheduled-timing.
Column-The separation is performed inside the column; therefore, it can be said that the column is the
heart of an LC system. The eluent used for LC varies from acidic to basic solvents. Most column housing
is made of stainless steel, since stainless is tolerant towards a large variety of solvents. However, for the
analysis of some analytes such as biomolecules and ionic compounds, contact with metal is not desired,
thus polyether ether ketone (PEEK) column housing is used instead.
Autosampler – draws samples from vials and injects them into the solvent flow
provided by the pump.
Detector – responds to the separated analytes emerging from the HPLC column and
produces a signal output for the software
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Column Oven – houses the HPLC column and keeps a stable temperature for
reproducible separations
Conclusion:
In this experiment we learn the High Performance Liquid Chromatography (HPLC) is a process of
separating components in a liquid mixture and its chromatrogram. And basic HPCL components
and setup of HPCL.
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EXPERIMENT-08
➢ Prepare a 3-4 page report on weather station available nearby you, in a group of 4
students from the same batch.
➢ The report should include the main features and working of weather station and
the description of pollutants to be measured and the instrumentation for the same.
History :
Meteorological Centre Ahmedabad was established with the aim of rendering quick and better
meteorological services to the Gujarat state. The Meteorological Observatory, Ahmedabad was
established on 18th January 1893 and the Meteorological Centre, Ahmedabad was established in
October 1974. To have efficient administrative control and better technical support, Meteorological
Centre Ahmedabad has 15 departmental and 7 Part Time synoptic observatories, 8 aeronautical
meteorological stations, one seismological observatories, one DWR and number of meteorological
observatories are established in Gujarat state.
ObservationalNetwork :
To collect meteorological data surface, upper air and remotely sensed data, number of
meteorological observatories, Automatic weather stations and Doppler Weather Radars are
established across the region. There are 19 manual observatories, 33 Automatic Weather Stations,
66 Automatic Rain gauges, 9 Agro Automatic Weather Stations and 4 upper air observatories. One
Doppler weather Radar and various other observing systems.
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Services :
With time the activities and responsibilities of Met Centre, Ahmedabad has increased in all
fields, for rendering the Meteorological services to the general public of the state of Gujarat,
and Union Territory of Diu, Daman and Dadra Nagar Haveli and concerned departments of
Central and State Governments like Agriculture, Irrigation, Power, Flood Control, Relief and
Rehabilitation, Roadways and Railways etc. All types of information relating to weather, viz.
weather bulletins, warnings etc. are issued to the public and State Govt., through the most
popular media like Press, Doordarshan and Akashwani for the safety of life and property.
Aviation Services to aeronautical communities are one of the valuable services rendered at
Ahmedabad Airport. Current Weather information and route weather forecasts are given to air
navigators. Quantitative precipitation Forecast (QPF) is issued on basin/ sub basin wise,
catchments/sub catchments-wise for the major rivers in Gujarat to Central Water Commission
during monsoon period. During non-monsoon period the task is to maintain all Hydro
meteorological rain gauge stations and preparation of various reports. Agro-Meteorological
Advisory is issued twice a week i.e. every Tuesday and Friday for the benefits of farmers of the
state. X-band radar is used to provide the Upper Air Weather Information which are very much
useful for the forecasting/nowcasting. Collection of rainfall of 252 DRMS stations and prepare
statistics of district wise rainfall of all 33 districts of the state on daily / weekly / Monthly /
Seasonal basis
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Objectives :
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EXPERIMENT-09
Ahmedabad being the largest city in Gujarat gets 1060 MLD water per day. It meets its
water requirements from 4 different water treatment plants after receiving the water from
the sources; Narmada Canal and Sabarmati River, of which 80% is met by the Kotarpur
Water Treatment Plant, Naroda using the gravitational force. The treatment plant was in
the construction process from 1984 to 1996 and started functioning in 1996.
The capacity of the plant is 650 MLD and over-loading capacity is 750 MLD.
A gravity line of 2500mm is used to connect Narmada canal and Kotarpur water works.
The water from Sabarmati River is taken by 2 Intake wells.
➢ The report should include the main features and working of the treatment plant
and the description of the process and the instrumentation for the same.
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PROCESS OF WATER TREATMENT
Following are the steps are involved in treatment of the water from the Input
(Intake bay) to the Output (Pumped out):
SCREENING
ALUM DOZING
COAGULATION
FLOCCULATION
SEDIMENTATION
FILTRATION
CHLORINATION
TREATMENT PROCEDURE
▪ Before the water enters the inlet bay, the water is first passed through
the screening where the big size particles are removed from water.
▪ In the inlet bay, alum is added which facilitates the process of
coagulation. The dosage of alum is decided after testing the water. The
turbidity of raw water is approximately 3000 NTU (Nephelometric
Turbidity Unit). Pre Chlorination process is done at the inlet bay.
▪ After the alum is added to the water at the inlet bay, the water is made
flowed to the distribution station from where the water is distributed to
12 clarifiers. Baffles are provided in structure so as to facilitate the
formation of hydraulic jumps which helps in proper mixing of alum in
water.
▪ The alum added at the inlet bay helps in formation of flocs in the
flocculator. The flocs formed settles in flocculator, which are then
transferred to sludge pit. The structure of the clarifier is such that a
sludge pit is formed in the centre. The clarifier has skimmers which
slowly rotates around the clarifier and pushes the floating material to
the sludge pit. The clarifiers are of diameter 50.10 metre. The sludge is
pumped and is discharged into the downstream of river. Almost 70%
purification takes places in clarifier.
▪
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KOTARPUR WATER WORKS LOCATION
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▪ The next process in the filtration. The water from the clarifier is then
flowed to the filter house, where there are 48 filter beds i.e. sand beds
of 750mm thickness. The water is allowed to flow through the sand beds
which further purifies the water.
▪ The filter beds are cleaned at an interval if 72 hours. The cleaning of
beds is done by pumping of air and then water from the bottom of the
bed.
▪ The water is then stored in the underground water tank. Air vents are
provided to release the air from the underground tank.
▪ The water is then pumped to the other water distribution stations.
▪ There are 10 no. of vertical turbine pumps which pumps water to the
city. Six of the pumps are working and other four are the standby
pumps. The capacity of each pump is 41 lakhs/hr.
Chlorine is added to the water before pumping. The final step is to
disinfect the water and maintain chlorine residuals that will remain in
the water as it travels through the distribution system.
▪ The chlorine consumption is approximately 1600 to 1700 kg per day.
QUALITY TEST
▪ The water is tested in site laboratory every two hours and record is
maintained.
▪ The turbidity of treated water is less than 1 NTU (Nephelometric
Turbidity Unit)
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EXPERIMENT-10
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CO, SMOKE DETECTING EMBEDDED SYSTEM
By using protius software we made the CO, SMOKE DETECTING EMBEDDED
SYSTEM.
Components lists for the CO, SMOKE detector
1) MQ 2 gas sensor
2) 16*2 l2c lcd
3) Arduino UNO
4) Jumper wires
CONNECTIONS
ARDUINO MQ2
A0 A0
5V VCC
GND GND
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CODE FOR THE PROJECT:
#include <MQ2.h>
#include <Wire.h>
#include <LiquidCrystal_I2C.h>
//I2C pins declaration
LiquidCrystal_I2C lcd(0x27, 2, 1, 0, 4, 5, 6, 7, 3, POSITIVE);
int Analog_Input = A0;
int lpg, co, smoke;
MQ2 mq2(Analog_Input);
void setup(){
Serial.begin(9600);
lcd.begin(16,2);//Defining 16 columns and 2 rows of lcd display
lcd.backlight();
mq2.begin();
}
void loop(){
float* values= mq2.read(true); //set it false if you don't want to print the values in the Serial
//lpg = values[0];
lpg = mq2.readLPG();
//co = values[1];
co = mq2.readCO();
//smoke = values[2];
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