Enzyme Mechanism

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Name: ______________________________

Course/Year: ________________________

Assessment Task 6.9

Lysozyme is an enzyme that catalyzes the hydrolysis of a glycoside (carbohydrate) through its (1,4) bond.
Given below are series of reactions which serve as its reaction mechanism. Put the appropriate curve arrows
to complete the mechanism.

Step 1: Binding of the substrate to the active site of the enzyme.


Step 2: Cleavage of the glycosidic bond, producing product 1 and oxonium ion.
Step 3: Bonding of oxonium ion with Asp-52.

Step 4: Reaction with water, release of the second product and return of enzyme to its native state.

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