TEST OF CARBOHYDRATES

S.NO EXPERIMENT OBSERVATION INFERENCE

1. Concentrated Sulphuric acid test: Charring with the Indicates the presence of
Small amount of sample is taken in smell of burnt sugar is carbohydrates.
the test tube and added few drops observed.
of conc.H2SO4

2. Fehling’s test: Reddish precipitate is Indicates the presence of reducing

2 ml of aqueous solution of formed. sugar.
carbohydrate is taken and added 1-
2ml of each of Fehling’s solution A
and Fehling’s solution B. The test
tube is kept in boiling water bath.
3. Benedict’s test: Reddish precipitate is Indicates the presence of reducing
1-2ml of aqueous solution of the formed. sugar.
carbohydrate is taken in a test tube
and added 1-2 ml of Benedict’s
reagent. The test tube is kept in
boiling water bath.
4. Tollen’s test: A shining silver mirror Indicates the presence of reducing
is formed. carbohydrate.
2-3ml of aqueous solution
of the given compound is taken in a
test tube ,added 2-3ml of tollen’s
reagent .The test tube is kept in a
boiling water bath for 10 minutes.

RESULT: The given organic compound is a carbohydrate.

 TEST OF PROTEINS

S.NO EXPERIMENT OBSERVATION INFERENCE

1. Bluish violet Indicates the presence of proteins.

To the dispersion of the substance colouration is
to be tested, added about 2ml of observed.
the NaOH solution, added 4-5 drops
of
1 % of CuSO4 solution. The mixture
is warmed for about five minutes.

2. Xanthoproteic test: Yellow colouration is Indicates the presence of proteins.

observed.
2 ml of egg albumin dispersion is
taken in a test tube ,added a few
drops of Conc.HNO3 and heated.

3. Ninhydrin test: Intense blue Indicates the presence of proteins.

2 ml of egg albumin dispersion is colouration is
taken in a test tube ,added 3-4 observed.
drops of Ninhydrin solution and the
contents is boiled.

4. Millon’s Test: White precipitate Indicates the presence of proteins.

2 ml of egg albumin dispersion and changes to red on
added 2 drops of Millon’s reagent is boiling.
added and boiled.

RESULT: The given organic compound is a protein.

 TEST FOR OILS AND FATS

S.NO EXPERIMENT OBSERVATION INFERENCE

1. Translucent Spot test: Appearance of Indicates the presence of of oil or fat.

A small amount of the given translucent spot on
substance is placed on a filter paper. the filter paper.
The filter paper is folded and The spot grows larger
pressed tightly. on heating and drying
the filter paper.

2. Solubility test:
Small amount of sample is shaken Sample is immiscible Indicates the presence of of oil or fat.
with water.

Small amount of sample is shaken

with alcohol Sample forms lower Indicates the presence of of oil or fat.
layer which dissolves
on heating.
3. Acrolein test: Irritating smell due to Indicates the presence of of oil or fat.
A few drops of the sample is taken the formation of
in a test tube and added few drops acrolein is observed.
of potassium bisulphate .The test
tube is heated .

RESULT: The given organic compound contains fat or oil.