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Gedi (Abelmoschus manihot L.) Leaf Extract Antioxidant Potential as FRAP

Method

Conference Paper · November 2015

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Muammar Fawwaz
Universitas Muslim Indonesia
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 Gedi (Abelmoschus manihot L.) Leaf Extract
Antioxidant Potential as FRAP Method
*Muammar Fawwaz, Nur Hikmah Hidayanti, Tadjuddin Naid
Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar
*Corres Author : fawwaz_pharm@yahoo.com

Gedi  plant  (Abelmoschus  manihot  L.),  

Malvaceae  family,  is  an  annual  plant  that  is  
trunked  upright  with  a  height  of  about  1.2  
to  1.8  m.  This  plant  contains  quercetin-­‐3-­‐O-­‐
robinobiosid,  hyperin,  Isoquercetin,  
gossipetin-­‐8-­‐O-­‐glucuronide,  and  myricetin  
(Liu,  2006).  The  antioxidant  activity  with  
DPPH  by  Esty  Dewi  A.  Marahena  Pratama   0.5  
Quarcetin  
(2013)  IC50  values  obtained  12.360  g  /  mL.   0.4  

Absorbance  
Base  on  these  data  it  is  necessary  to  test  the   0.3   y  =  0.0081x  +  0.1295  
  R²  =  0.98843  
antioxidant  activity  continued  with  FRAP   0.2  
method.   0.1  
0  
0   20   40   60  
Concentration  

Quarcetin (1000 ppm)  

Etanolic  Extract  of  Gedi  Leaves  (1000  ppm)   Made  a  series  of  serial  
dilutions  of  a  standard  
Pipette 1 mL solution  of  quercetin  
+ 1 mL fosfat buffer 0,2 M (pH 6,6)
+ 1 mL K3Fe(CN)6 1% 10 ppm 20 ppm 30 ppm 40 ppm
o
Incubated 20 minutes (50 C)
+ 1 mL TCA 10 %
Centrifuge at 3000 rpm (10 minutes)
 

Sample   Absorb Antioxidant   Avarage  

Residual Supernatant Replica ance   Capacity  (µg   Antioxidant  
Pipette 1 mL tion   Q  /  g  sample)   Capacity  (µg  
+ 1 mL aquades Q  /  g  sample)  
+ 0,5 mL FeCl3 0,1%
I   0,206   41,046    
The complex is green
to blue (blue berlin) II   0,267   48,039   45,033  
Absorbance measured at 700 nm
in spectrophotometer Uv-Vis
III   0,249   46,014  

Data

Conclusion

International  Seminar  of  

Biocatalyst  for  food,  energy  
and  medical  applications.  
Bandung  10  November  2015.  
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