THE FIRST PREBOARD EXAMINATION 2022-2023

CLASS XII (ISC)

BIOTECHNOLOGY PAPER-1 (THEORY)
Time: Three hours Maximum marks:70
You are given 15 minutes of reading time.
This paper consists of three parts, Section A, Section B and Section C.
Answer all questions in all sections.
Internal choices have been provided in two questions of Section B and one question of Section C.
SECTION A [14Marks]
Question 1
i. RNA dependent DNA polymerase was first discovered in _________. [1]
ii. _________ blotting technique is used to isolate proteins. [1]
iii. A detergent was manufactured by changing the nucleotide sequence [1]
to a desirable one. Which of the following techniques have been used?
a. Southern blotting c. PCR
b. Site directed mutagenesis d. Northern blotting
iv. Which instrument can be used for counting of viable plant cells? [1]
a. Hemocytometer c. Colony counter
b. CO2 incubator d. Evan’s blue staining
v. State whether the following statements are TRUE or FALSE. Give a reason in support of your
answer. [2]
a. Introns are found in the circular chromosome of prokaryotes.
b. A collection of cloned DNA fragments that represents the entire genome is called gene library.
vi. Define: [2]
a. Proteomics
b. Cryopreservation
vii. Differentiate between each of the following: [2]
a. Androgenesis and Gynogenesis
b. Blunt ends and sticky ends of restriction enzymes
viii. Expand each of the following: [2]
a. SDS-PAGE b. HEPA
ix. Assertion: Agrobacterium tumefaciens is called natural genetic engineer [1]
as it can transfer DNA from one plant to another.
Reason: The T-DNA is disarmed for insertion of foreign genes.
a. Both Assertion and Reason are true and Reason is the correct explanation of Assertion.
b. Both Assertion and Reason are true but Reason is not the correct explanation for Assertion.
c. Assertion is true but Reason is false.
d. Both Assertion and Reason are false.
x. Assertion: The hnRNA undergoes post transcriptional modification [1]
in eukaryotes.
Reason: Splicing occurs to protect DNA polymerases from destruction.
 a. Both Assertion and Reason are true and Reason is the correct explanation of Assertion.
b. Both Assertion and Reason are true but Reason is not the correct explanation for Assertion.
c. Assertion is true but Reason is false.
d. Both Assertion and Reason are false.
                                                          SECTION B [28 marks]
Question 2
Write short notes on: [4]
a. Golden Rice
b. Chargaff’s Equivalence Rule
Question 3
Briefly explain the following: [4]
a. Search tools used in bioinformatics
b. Batch culture
OR
a. Sterilization of explants
b. Sterilization of nutrient media
Question 4
State any two differences between the following: [4]
a. YAC and BAC
b. BLAST and FASTA
Question 5
i. Give an account of any four global bioinformatics databases. [4]
OR
ii. Explain the different levels of stem cells.
Question 6
Give reasons for the following: [4]
a. Cold trypsinization is preferred to warm trypsinization for primary cell culture.
b. tRNA needs to be acylated for protein synthesis.
Question 7
Describe the process of Southern blotting. [4]
Question 8
Give an account of Somatic hybridization in plants. [4]
SECTION C [28 marks]
Question 9
i. Describe the semi conservative nature of DNA replication. [4]
ii. Explain the different types of nutrient media for microbial culture. [3]
OR
i. Describe the steps in monolayer culture of animal cells. [4]
ii. Write any three achievements of HGP. [3]
Question 10
i. Describe any four methods of transfer of recombinant DNA into a host cell. [4]
ii. Explain the criteria of selection of organisms for genome sequencing. [3]
Question11
i. Study the figure given below and answer the questions that follow: [4]
a. Which regulatory mechanism is depicted in the above diagram?
b. Identify B, C and D.
c. What is the role of A?
d. State one difference between the above and trp mechanism.

ii. The figure given below is an important process. Study the figure and answer the questions that
follow.

i. What is the process shown above and who devised it?

ii. Identify A, B and C.
iii. State the thermostable enzyme used in the process.

Question 12
Read the following passage and answer the questions that follow:
Recombinant vaccines hold greater promise than traditional attenuated vaccines. A small portion of
DNA is taken from the virus or bacterium against which we want to protect and inserted into the
manufacturing cells. Many such vaccines have been prepared for Hepatitis B, meningitis, Covid 19
etc.
i. Name the gene that codes for Hepatitis B? [1]
ii. How is the Hepatitis B vaccine prepared using rDNA technology? [3]
iii. What are edible vaccines and how are they different from recombinant vaccines? [3]