A

PROJECTREPORT
On
SAFETYI
NTHECHEMI CALLABORATORY
To
DEPARTMENTOFCHEMI
STRY(
ORGANI
CCHEMI
STRYPRACTI
CAL)

Sessi
on-
2021-
2022

SubmittedTo Submitt
edBy
Dr.DhruvaKumar VanshThakral(10815)
(Assi
stantProf
essor
) SheenuGarg(10811)
M.Sc-I,Chemistry

DEPARTMENTOFCHEMI STRY,GURUNANAKCOLLEGE,
BUDHLADA-151502
(Undert
heManagementofS.
G.P.C.Sr
iAmr
it
sarSahi
b)
 Cont
ent
s

Chapt
er-SAFETYI
NTHECHEMI
CALLABORATORY

1.
1Int
roduct
ionoft
heLabor
ator
ySaf
ety

1.
2 Saf
etyGui
del
ines

1.
2.1 Wearey
epr
otect
ion.

1.
2.2 Dr
essappr
opr
iat
elyf
orl
ab.

1.
2.3 Wearappr
opr
iat
egl
oves.

1.
2.4 Keept
hel
abcl
eanandneat
.

1.
2.5 Per
for
m onl
yaut
hor
izedexper
iment
s.

1.
2.6 Donotwor
kal
one.

1.
2.7 Tr
eatal
ll
abor
ator
yreagent
sasi
ftheyar
ehazar
dous.

1.
2.8 St
oreanddi
sposeofl
abor
ator
yreagent
sasdi
rect
ed.

1.
2.9 Knowhowt
otr
anspor
tchemi
cal
ssaf
ely
.

1.
2.10Donotbr
ingf
ood,
dri
nks,
orcosmet
icsi
ntot
hel
abor
ator
y.

1.
2.11I
mmedi
atel
yrepor
tal
li
nci
dent
stot
hel
abor
ator
yinst
ruct
or.

1.
2.12Knowt
hel
ocat
ionanduseofal
lsaf
etyequi
pment
.

1.
2.13Bef
ami
l
iarwi
tht
heexper
imentbef
orebegi
nni
ngt
hel
ab.

1.
2.14Wor
kint
hel
abor
ator
yasi
fsomeoneel
sei
sgoi
ngt
ohav
e

anacci
dent
.

1.
2.15Readl
abel
scar
eful
l
y

1.
2.16Ask“
dumb”quest
ions.

1.
2.17Don’
trush.

1.
2.18Label
vial
sandf
lasks.
1.
2.19Knowwhatt
odoi
ntheev
entofanemer
gency
.

1.
2.20I
nfor
myouri
nst
ruct
orofanyspeci
almedi
cal
condi
ti
ons.

1.
3 Toxi
cChemi
str
yExposur
e

1.
4 Saf
etySi
gns
1.
1Int
roduct
ionoft
heLabor
ator
ySaf
ety
Ther
esponsi
bil
ityf
orl
absaf
etyr
est
swi
theachandever
yst
udenti
nthe

l
abor
ator
y.Youmustusecommonsenseandwor
kcar
eful
lyt
oavoi
dchemi
cal

spi
l
ls,
brokengl
asswar
e,andf
ir
es.Thi
sensur
esnotonl
yyourownsaf
ety
,butal
so

t
hatofy
ourl
abmat
es.Knowt
hehazar
dsofeachchemi
caly
ouusesot
haty
ou

wi
l
lknowwhatl
evelofcaut
iont
ousewhenhandl
i
ngi
t.I
fyoudot
his,
youwi
l
lnot

beexposedt
oahar
mfulamountofanychemi
caldur
ingyouryeari
nor
gani
c

chemi
str
ylab.

I
fanacci
dentdoeshappen,
youmustt
akest
epst
opr
eventf
urt
heri
njur
y.Most

acci
dent
sar
emi
nor
,andmet
hodsofdeal
ingwi
tht
hem ar
edet
ail
edi
nthe

sect
ionsbel
ow.I
ntheev
entofaser
iousacci
dent
,remembert
hati
njur
edpeopl
e

ar
eof
teni
nshockandar
eunabl
etohel
pthemsel
ves.Youshoul
dbepr
epar
edt
o

hel
pyournei
ghbori
ncaseofanacci
dent
.Amat
terofsecondscanbecr
it
ical
.

1.
2Saf
etyGui
del
ines
Theor
gani
cchemi
str
ylabor
ator
yhast
hepot
ent
ialt
obev
erydanger
ous.Thi
sis

whyi
tisext
remel
yimpor
tantt
haty
ouf
oll
owal
lsaf
etygui
del
i
neswheny
ouar
ein

t
hel
abor
ator
y.Asanet
hicalchemi
str
ylabor
ator
yst
udenty
oumustmakesur
e

t
haty
ouunder
standandf
oll
owal
lsaf
etypr
ecaut
ions.I
gnor
ingsaf
etypr
ecaut
ions

mayr
esul
tinaser
iousi
njur
ytoy
our
sel
foranot
herst
udent
.Anyst
udentwor
king

i
nanunsaf
emanneri
sadangert
oot
herst
udent
sandwi
llbeaskedt
oleav
ethe

l
abor
ator
y.Al
way
sadher
etot
hef
oll
owi
ngsaf
etygui
del
i
nes:
1.
2.1Wearey
epr
otect
ion.

Chemi
cal
scancauseser
iousdamaget
oyoureyes,i
ncl
udi
ngbl
indness.To

pr
eventchemi
cal
sandot
herhazar
doussubst
ancesf
rom comi
ngi
ncont
actwi
th

y
ourey
es,
saf
etygl
assesorgoggl
esmustbewor
natal
lti
mesy
ouorsomeone

el
sei
swor
kingi
nthel
abor
ator
y.(
Ify
oumustadj
ustorcl
eany
ourey
epr
otect
ion,

st
epout
sideoft
hel
abor
ator
ytodoso.
)Goggl
esof
fert
hebestpr
otect
ionandar
e

par
ti
cul
arl
yrecommendedt
oguar
dagai
nstspl
ashesandexpl
osi
ons.

1.
2.2Dr
essappr
opr
iat
elyf
orl
ab.

Thel
essski
nthati
s exposed,t
he bet
ter
!Sandal
s ar
e notal
lowedi
nthe

l
abor
ator
ybecauset
heyexposeyourt
oest
ochemi
calspi
ll
sandbr
oken

gl
asswar
e.Shoesmustbecl
ose-
toedandof
fert
hebestpr
otect
ionwhent
heyar
e

madef
rom anon-
por
ousmat
eri
al.Long-
sleevedshi
rt
s/bl
ousesandl
ongpant
s

ar
erecommended.I
fyouchooset
owearashi
rtt
hatexposesy
ourmi
dri
ff,
you

mustwearal
abcoat
.Labcoat
sar
eal
sor
ecommendedt
opr
otectcl
othi
ngyou

val
ue.Avoi
dloose-
fit
tingcl
othi
ngandaccessor
ies(
e.g.
,scar
ves,t
ies,l
ong

neckl
aces,
etc.
)becauset
heymaycausespi
l
lsorcomei
ncont
actwi
thhazar
dous

chemi
cal
s.Hai
rthati
slongert
hanshoul
derl
engt
hmustbet
iedback.

1.
2.3.Wearappr
opr
iat
egl
oves.

Gl
ovesar
erequi
redatal
lti
meswhen y
ou ar
e handl
ing chemi
cal
sandi
tems

t
hathav
ebeenexposedt
ochemi
cal
s.Keepi
nmi
ndt
hatdi
ff
erentgl
ovespr
otect

agai
nstdi
ffer
entt
ypesofchemi
cal
s.However
,onceyouhavegl
oveson,t
his

doesnotmeant
hatyouar
efr
eet
otouchever
ythi
ng!Forexampl
e,youdonot

wantt
oruby
oureyewi
thagl
ovedhandonceyouhavehandl
edchemi
cal
s.A

goodr
uleoft
humbi
stonott
ouchany
thi
ngwi
thy
ourgl
ovest
haty
oudonotwant

chemi
cal
son.Obt
ainanewpai
rofgl
ovesi
ftheydev
elopar
iport
ear
.
1.
2.4.Keept
hel
abcl
eanandneat
.

Av
oidcl
utt
eri
ngt
hel
abbenchorhood.Cl
utt
ercanl
eadt
ospi
l
ls,sor
etur
nit
ems

pr
ompt
lyt
othei
rpr
operl
ocat
ions.
Donotpl
aceper
sonali
tems,
suchasbagsand

backpacks,ont
hef
loorast
heymaycauseot
her
stot
rip.Pr
ompt
lynot
ifyt
he

l
abor
ator
yinst
ruct
orofanyspi
l
lsandcl
eant
hem upasi
nst
ruct
ed.Spi
l
lsmustbe

cl
eanedupassoonaspossi
blet
opr
eventot
her
sfr
om exposur
etot
hespi
ll
ed

chemi
cal
.Inf
ormt
hel
abor
ator
yinst
ruct
orofanycondi
ti
onst
hatseem unsaf
e.

1.
2.5.Per
for
m onl
yaut
hor
izedexper
iment
s.

Unaut
hor
izedexper
iment
ati
on may expose you andyourl
abmat
est
o

unf
oreseenhazar
ds.

1.
2.6.Donotwor
kal
one.

Al
wayshavesomeonepr
esenti
nthel
abor
ator
ythatknowswhatt
odoi
nthe

ev
entofanemer
gency
.

1.
2.7.
Treatal
llabor
ator
yreagent
sasi
ftheyar
ehazar
dous.

Of
ten,
notal
lhazar
dsar
eknownaboutachemi
cal
youmaybewor
kingwi
th,
so

al
waysusemaxi
mum pr
ecaut
ions.I
fyouspi
llachemi
calonyourski
n,

i
mmedi
atel
yri
nsei
twi
thwat
er,
andt
hennot
if
yyourl
abor
ator
yinst
ruct
or.Al
way
s

washy
ourhandst
hor
oughl
ywi
thsoapordet
ergentbef
orel
eav
ingt
hel
abor
ator
y,

eveni
fyouweargl
oves.Appr
opr
iat
elabel
sont
her
eagentbot
tleswi
llof
ten

i
ndi
cat
especi
alhazar
ds.

1.
2.8.St
oreanddi
sposeofl
abor
ator
yreagent
sasdi
rect
ed.

Paycar
efulat
tent
iont
othel
ab manualandt
oyouri
nst
ruct
oronhowt
ost
ore

anddi
sposeofr
eagent
s.Forexampl
e,somer
eagent
sar
eext
remel
yfl
ammabl
e

andmustbest
oredi
naf
lammabl
ecabi
netwhennoti
nuse.Ot
herr
eagent
smust

bekeptawayf
rom chemi
cal
sthatt
heyar
eincompat
ibl
ewi
th.Manyr
eagent
s
cannotbedi
sposedofdownt
hedr
ainast
heymaybet
oxi
ctot
heenv
ironment
.If

y
ouar
einst
ruct
edt
ouseawast
econt
ainerf
ordi
sposalofar
eagent
,insur
ethe

wast
econt
aineri
scl
osedaf
tery
ouar
efi
nishedt
opr
eventexposur
etonoxi
ous

f
umes.Pr
ompt
lynot
if
ythel
abor
ator
yinst
ruct
orofanyspi
ll
sandcl
eant
hem as

i
nst
ruct
ed.Spi
l
lsmustbecl
eanedupassoonaspossi
blet
opr
eventot
her
sfr
om

bei
ngexposedt
othespi
l
ledchemi
cal
.

1.
2.9.Knowhowt
otr
anspor
tchemi
cal
ssaf
ely
.

I
fyoumustt
ranspor
tachemi
calout
sideoft
hel
abor
ator
y,t
hechemi
calmustbe

i
nacl
osedcont
ainer(
i.
e.,
noopenbeaker
s).Addi
ti
onal
ly,l
argebot
tlesand

par
ticul
arl
yhazar
douschemi
cal
smustbet
ranspor
tedi
nasaf
etycar
rier
.

1.
2.10.Donotbr
ingf
ood,
dri
nks,
orcosmet
icsi
ntot
hel
abor
ator
y.

Eat
ing,dr
inki
ng,orappl
yingcosmet
icsi
nthel
abor
ator
ycani
ntr
oducet
oxi
cor

cor
rosi
vechemi
cal
sint
oyoursyst
em.Youpar
ticul
arl
ywantt
oavoi
dany

cont
ami
nat
iont
oyourmout
hor
eyes.

1.
2.11.I
mmedi
atel
yrepor
tal
linci
dent
stot
hel
abor
ator
yinst
ruct
or.

Ani
nci
denti
sanyi
njur
yorsi
tuat
iont
hatmi
ghtr
esul
tinani
njur
y.Thel
abor
ator
y

i
nst
ruct
orwi
llbestknowhowt
ohandl
ethesi
tuat
ionandmayal
souset
he

i
nfor
mat
iony
oupr
ovi
det
ohel
pot
herst
udent
sav
oidasi
mil
ardi
ff
icul
ty.

1.
2.12.Knowt
hel
ocat
ionanduseofal
lsaf
etyequi
pment
.

Al
llabor
ator
ieswi
llhav
ean ey
ewashst
ati
on,
saf
etyshower
,fumehood,
afi
re

ext
ingui
sher
,andacar
rierf
ort
hesaf
etr
anspor
tofchemi
cal
s.Al
soknowt
he

l
ocat
ionoft
henear
estemer
gencyal
arm.

1.
2.13.Bef
ami
li
arwi
tht
heexper
imentbef
orebegi
nni
ngt
hel
ab.

Paypar
ti
cul
arat
tent
iont
ocaut
ionsgi
veni
nthepr
ocedur
eandbyt
hel
abor
ator
y

i
nst
ruct
or.Lear
nabouthazar
dsassoci
atedwi
tht
hechemi
cal
syouwi
llbeusi
ng
byl
ooki
ngupandr
eadi
ngt
hei
rMat
eri
alSaf
etyDat
aSheet(
MSDS)
.Addi
ti
onal
ly
,

beawar
eofpr
ecaut
ionst
obet
akenwi
thanyspeci
alequi
pmenty
oumaybeusi
ng.

1.
2.14.Wor
kint
hel
abor
ator
yasi
fsomeoneel
sei
sgoi
ngt
ohav
eanacci
dent
.

Justbecausey
ouar
efol
lowi
ngal
loft
hesaf
etypr
ecaut
ionsdoesn’
tmeant
hat

y
ournei
ghbor
is!

1.
2.15.
Readl
abel
scar
eful
ly
.

Onl
yuset
hecor
rectchemi
cal
sint
hepr
operconcent
rat
ions.Thewr
ongchemi
cal

orconcent
rat
ioncoul
dresul
tinav
iol
ent
react
ion.

1.
2.16.
Ask“
dumb”quest
ions.

I
fyouar
enotsur
eabouthowt
opr
oper
lyper
for
m apr
ocedur
eorhandl
ea

chemi
cal
,asky
ouri
nst
ruct
or.Regar
dlessofwhat
everpr
econcei
vednot
ionsy
ou

mayhav
e,aski
ngquest
ionsdoesnotmakey
oul
ookst
upi
d,especi
all
ywhen

saf
etyi
saconcer
n!

1.
2.17.
Don’
trush.

I
fyouar
eanxi
oust
oleavel
abear
lyorwor
kinghur
riedl
ytocat
chup,youmay

negl
ecti
mpor
tantsaf
etypr
ecaut
ions.Al
way
smakesaf
etyapr
ior
it
y.

1.
2.18.Labelv
ial
sandf
lasks.

I
fyoudonotl
abel
avi
alandt
henf
orgetwhati
tcont
ains,
youwi
l
lnotknowhowt
o

pr
oper
lyhandl
eanddi
sposeofi
tscont
ent
s.

1.
2.19.
Inf
ormy
ouri
nst
ruct
orofanyspeci
almedi
calcondi
ti
ons.

I
fyouhaveamedi
calcondi
ti
ont
hatmi
ghtbeaf
fect
edbyl
abor
ator
ywor
k(e.
g.,

al
ler
gies)ort
hatmi
ghtaf
fectsaf
eper
for
manceofl
abor
ator
ywor
k(e.
g.,
sei
zur
e

di
sor
der
s),i
nfor
myouri
nst
ruct
ori
mmedi
atel
y.Addi
ti
onal
ly,st
udent
swhoar
e

pr
egnantorbr
eastf
eedi
ngmustcont
actt
hei
nst
ruct
orpr
ior
tot
hei
nit
iat
ionof

l
aborat
orywor
k.Chemical
susedint
helabor
ator
ymaybepar
ti
cul
arl
yhar
mful
to
dev
elopi
ngfet
usesandyounginf
ant
s.
1.
2.20.Knowwhatt
odoi
ntheev
entofanemer
gency
.

Pr
omptr
esponset
oani
nci
dentcanmaket
hedi
ff
erencebet
weenami
norev
ent

andper
manenti
njur
y.Youshoul
dbef
ami
li
arwi
thact
ionst
otakei
ntheev
entof

ani
nci
dent
,aswel
last
hel
ocat
ionoft
heSt
udentHeal
thCent
er.

1.
3Toxi
cChemi
calExposur
e
Oneoft
hel
eastpr
edi
ctabl
e,mostdanger
ousr
iskst
hatper
sonnelf
acei
nsi
dea

l
abor
ator
yist
het
oxi
cit
yofvar
iouschemi
cal
s.I
nthechemi
str
ylabor
ator
y,no

subst
ancei
sent
ir
elysaf
eandal
lchemi
cal
sresul
tinsomet
oxi
cef
fect
sifal
arge

enoughamountoft
hesubst
ancecomesi
ncont
actwi
thal
ivi
ngsy
stem.Many

chemi
cal
sdi
spl
aymor
ethanonet
ypeoft
oxi
cit
y.Tabl
eES.
1li
stst
hemost

commoncl
assesoft
oxi
csubst
ances.
1.
4Saf
etysi
gns
Thepur
poseoft
hesaf
etysi
gnsy
stem i
sthatofat
tract
ingt
heat
tent
iont
oobj
ect
s

andsi
tuat
ionst
hatcoul
dcausedet
ermi
neddanger
sinaqui
ckandeasi
ly

under
standabl
ewayandi
npar
ti
cul
art
opr
ohi
bitdanger
ousbehavi
our
;war
nthe

peopl
eexposedofar
iskordanger
;pr
escr
ibesaf
ebehav
iourf
orsaf
etypur
poses;

suppl
yindi
cat
ionsr
elat
ivet
othesaf
etyexi
tsandt
otheai
dandr
escuemeans;

i
ndi
cat
epr
event
ionandsaf
etyel
ement
s.

*
Ther
egul
ati
onsdef
inet
heshapeoft
henot
icesi
gn,
thecol
our
soft
hebor
der
s,of

t
hebackgr
oundandoft
hepi
ctogr
amsont
hebasi
soft
het
ypeofsi
gn:

-pr
ohi
bit
ionsi
gnsar
eroundwi
thar
edbor
der
,ar
edt
ransv
ersalbarandabl
ack

pi
ctogr
am onawhi
tebackgr
ound.Ther
edcol
ourmustcov
eratl
east35%oft
he

sur
faceont
hist
ypeofsi
gn;

-war
ningsi
gnsar
etr
iangul
arwi
thabl
ackpi
ctogr
am onay
ell
owbackgr
ound.The

yel
low col
ourmustcoveratl
east50% oft
hesur
faceoft
hist
ypeofsi
gn;

-pr
escr
ipt
ionsi
gnsar
eroundwi
thawhi
tepi
ctogr
am onal
i
ghtbl
uebackgr
ound.

Thel
i
ghtbl
uecol
ourmustcov
eratl
east50%oft
hesur
faceoft
hist
ypeofsi
gn;

-ai
dandr
escuesi
gnsar
esquar
eorr
ect
angul
ari
nshapewi
thawhi
tepi
ctogr
am

onagr
eenbackgr
ound.Thegr
eencol
ourmustcov
eratl
east50%oft
hesur
face

oft
hist
ypeofsi
gn;

-f
ir
epr
otect
ionsi
gnsar
esquar
eorr
ect
angul
ari
nshapewi
thawhi
tepi
ctogr
am

onar
edbackgr
ound;

-i
nfor
mat
ionsi
gnsar
esquar
eorr
ect
angul
ari
nshapewi
thawhi
tepi
ctogr
am on

al
i
ghtbl
uebackgr
ound.
Ar
rangementoft
hesi
gnsi
nthechemi
call
abor
ator
ies
Whenar
rangi
ngt
henot
icesi
gnswi
thsaf
etyi
ndi
cat
ionsi
nthechemi
cal

l
abor
ator
ies,
iti
snecessar
ytof
oll
owt
hesei
ndi
cat
ions:

-At
tachasi
gnboar
datt
heent
rancet
othel
abor
ator
ywhi
chst
atest
hemai
n

pr
ohi
bit
ions,
prescr
ipt
ionsandwar
nings(
anexampl
eisgi
veni
nthef
igur
e).

-Att
achthenecessarysi
gnboardspert
aini
ngtowarni
ngsandhazardst
oeach
cabi
net
,ref
ri
geratororanyotherst
ruct
urecont
aini
ngchemical
product
s.

-At
tachsi
gnboar
dsper
tai
ningt
othemai
npr
ohi
bit
ions,
hazar
ds,
war
ningset
c.i
n

t
hel
abor
ator
yinsi
det
hepr
emi
ses.
 REFERENCES
1.Googl
e.co.
in
2.http:
//ht
tps:
//l
aney.edu/chel
i-
fossum/wp-
content/
uploads/si
tes/210/2012/01/1-
Lab-
Saf
ety
-i
n-t
he-
Organic-
Lab.pdf
3.Armour,M.A.2003.HazardousLaborat
oryChemi
cal
s
Disposal
Guide,Thi
rdEdit
ion.BocaRaton,Fl
a.:
CRCPress.
4.Lunn,G.,
andE.B.Sansone.1990.Destr
uct
ionofHazar
dous
Chemicalsint
heLaborat
ory.NewYor k:
JohnWiley&Sons.