Amoz

BIOO MaxSignal® ELISA Analysis Program in Excel (with BIOO Specified Standards)
Use worksheet "BIOO Specified Standards" only when you follow the standards specified in BIOO manuals. All the standards are preset and
automatically entered per our protocol. BIOO standards may change from time to time, please double check with product manual for confirmation,
or use worksheet "Cust. Specified Standards" to input.
Step 1 Fill in "Test Notes"
Step 2 Name the samples in "Plate Layout Diagram" (Only in blue area. DO NOT INPUT OD VALUES HERE)
Step 3 Input OD values
Step 4 Check on "Solvent Blank Sample"
Step 5 Input Standard Concentration Values
Step 6 Input Positive Cut-off Value
Step 7 Input Dilution Factor
Step 8 Review Test Results
STEP 1: TEST NOTES OTHER NOTES
Test Kit Name: 1020-03 - FURALTADONE (AMOZ)

1020-03 - FURALTADONE (AMOZ)
Kit Lot #: 102003112916$64975146799
Sample Type: SHRIMP
Check in Time: 02/12/17
Detection Time: 06:11:52 AM
Test Technician: NITA S.S
QA Technician: NITA S.S
STEP 2: PLATE LAYOUT DIAGRAM

(Name the samples in blue area ONLY. Do NOT input OD values in this table)
1 2 3 4 5 6 7 8 9 10 11 12
A Std1 Sample3 Sample11 Sample19 Sample27 Sample35 Sample43 Sample51 Sample59 Sample67 Sample75 Sample83
B Std2 Sample4 Sample12 Sample20 Sample28 Sample36 Sample44 Sample52 Sample60 Sample68 Sample76 Sample84
C Std3 Sample5 Sample13 Sample21 Sample29 Sample37 Sample45 Sample53 Sample61 Sample69 Sample77 Sample85
D Std4 Sample6 Sample14 Sample22 Sample30 Sample38 Sample46 Sample54 Sample62 Sample70 Sample78 Sample86
E Std5 Sample7 Sample15 Sample23 Sample31 Sample39 Sample47 Sample55 Sample63 Sample71 Sample79 Sample87
F Std6 Sample8 Sample16 Sample24 Sample32 Sample40 Sample48 Sample56 Sample64 Sample72 Sample80 Sample88
G Sample1 Sample9 Sample17 Sample25 Sample33 Sample41 Sample49 Sample57 Sample65 Sample73 Sample81 Sample89
H Sample2 Sample10 Sample18 Sample26 Sample34 Sample42 Sample50 Sample58 Sample66 Sample74 Sample82 Sample90
BIOO MaxSignalTM ELISA Detection Analysis . 1

STEP 3: OD450 INPUT
1 2 3 4 5 6 7 8 9 10 11 12
A 2.5760 2.3040 Sample11 Sample19 Sample27 Sample35 Sample43 Sample51 Sample59 Sample67 Sample75 Sample83
B 2.1040 2.4110 Sample12 Sample20 Sample28 Sample36 Sample44 Sample52 Sample60 Sample68 Sample76 Sample84
C 1.6180 2.3270 Sample13 Sample21 Sample29 Sample37 Sample45 Sample53 Sample61 Sample69 Sample77 Sample85
D 1.0710 2.2470 Sample14 Sample22 Sample30 Sample38 Sample46 Sample54 Sample62 Sample70 Sample78 Sample86
E 0.5380 2.3180 Sample15 Sample23 Sample31 Sample39 Sample47 Sample55 Sample63 Sample71 Sample79 Sample87
F 0.1860 Sample8 Sample16 Sample24 Sample32 Sample40 Sample48 Sample56 Sample64 Sample72 Sample80 Sample88
G 2.4780 Sample9 Sample17 Sample25 Sample33 Sample41 Sample49 Sample57 Sample65 Sample73 Sample81 Sample89
H 2.2360 Sample10 Sample18 Sample26 Sample34 Sample42 Sample50 Sample58 Sample66 Sample74 Sample82 Sample90
STEP 4: PLEASE DEFINE SAMPLE 1 - IS IT A SOLVENT BLANK SAMPLE? Yes

If you have set B as the solvent blank sample, the result of the solvent control will be automatically substracted from the Yes
sample results. You can also use these two wells for real sample testing, in which case you should select "No"
STEP 5: STANDARDS CONCENTRATION VALUES

(From Low to High)
STD. NO. Std Conc. Log Conc. OD450-1 B/B0*
Std1 0.000 2.576 1.000
Std2 0.025 (3.689) 2.104 0.817
Std3 0.100 (2.303) 1.618 0.628
Std4 0.400 (0.92) 1.071 0.416
Std5 1.600 0.47 0.538 0.209
Std6 6.400 1.86 0.186 0.072
* Note: b/b0 =absorbance standard (or sample)/absorbance zero standard
STEP 6: POSITIVE CUT-OFF VALUE 0.05 In ppb or ppm, input

STEP 7: SAMPLE DILUTION FACTOR 2.00 Check manual for input
Linear Regression
Standard Curve Parameters
Slope (0.14)
Intercept 0.30
Correl (1.00)
PLEASE DOUBLE CHECK:

-- Solvent blank sample (Yes or No) checked
-- Standard concentration values checked (from low to high)
-- Positive Cut-off Value checked
-- Sample Dilution Factor checked

STEP 8: TEST SUMMARY
Sample Name OD-1 B/B0 Results Pass/Fail Note Test Kit Name: 1020-03 - FURALTADONE (AMOZ)
Sample1 2.478 0.962 0.0000 NA Kit Lot #: 102003112916$64975146799

Sample2 2.236 0.868 0.000 PASS Sample Type: SHRIMP
Sample3 2.304 0.894 0.000 PASS Check in Time: 02/12/17
Sample4 2.411 0.936 0.000 PASS Detection Time: 06:11:52 AM
Sample5 2.327 0.903 0.000 PASS Test Technician: NITA S.S
Sample6 2.247 0.872 0.000 PASS QA Technician: NITA S.S
Sample7 2.318 0.900 0.000 PASS
Sample8 Sample8 #VALUE! #VALUE! #VALUE! OTHER NOTES
Sample9 Sample9 #VALUE! #VALUE! #VALUE! 0
Sample10 Sample10 #VALUE! #VALUE! #VALUE!
Sample17 Sample17 #VALUE! #VALUE! #VALUE! Standard Curve
Sample18 Sample18 #VALUE! #VALUE! #VALUE! 100%
90%
Relative Absorbance
R² = 0.995199400155628
Sample24 #VALUE! #VALUE! #VALUE!
60%
Sample24
40%
Sample28 #VALUE! #VALUE! #VALUE! 30%
Sample28
10%
Sample31 Sample31 #VALUE! #VALUE! #VALUE! 0.0 0.1 1.0 10.0 100.0
Sample33 Sample33 #VALUE! #VALUE! #VALUE! Standard Concentration (ppb)


BIOO MaxSignal® ELISA Analysis Program in Excel (with Customer Specified Standards)
Use worksheet "Customer Specified Standards" if you want to use the standards rather than those specified in BIOO manuals. BIOO does NOT
recommend you do so in that the performance of the kit is designed to meet our strict QC in accordance with our protocol specifics. You may also
use this worksheet if preset values are not up to date.
Step 1 Fill in "Test Notes"
Step 2 Name the samples in "Plate Layout Diagram" (Only in blue area. DO NOT INPUT OD VALUES HERE)
Step 3 Input OD values
Step 4 Check on "Solvent Blank Sample"
Step 5 Input Standard Concentration Values
Step 6 Input Positive Cut-off Value
Step 7 Input Dilution Factor
Step 8 Review Test Results
STEP 1: TEST NOTES OTHER NOTES
Test Kit Name:

1024-051024-05 - FLUOROQUINOLONE
- FLUOROQUINOLONE
Kit Lot #:
Sample Type:
Check in Time:
Detection Time:
Test Technician:
QA Technician:
STEP 2: PLATE LAYOUT DIAGRAM

(Name the samples in blue area ONLY. Do NOT input OD values in this table)
1 2 3 4 5 6 7 8 9 10 11 12

STEP 3: OD450 INPUT
1 2 3 4 5 6 7 8 9 10 11 12
STEP 4: PLEASE DEFINE SAMPLE 1 - IS IT A SOLVENT BLANK SAMPLE? Yes

If you have set B as the solvent blank sample, the result of the solvent control will be automatically substracted from the Yes
sample results. You can also use these two wells for real sample testing, in which case you should select "No"
STEP 5: STANDARDS CONCENTRATION VALUES

(From Low to High)
STD. NO. Std Conc. Log Conc. OD450-1 B/B0*
Std1 0.000 Std1 #VALUE!
Std2 0.100
0.100 (2.303) Std2 #VALUE!
Std3 0.100
0.10 (2.303) Std3 #VALUE!
Std4 0.100
0.100 (2.30) Std4 #VALUE!
Std5 1.600
1.600 0.47 Std5 #VALUE!
Std6 0.800
0.800 (0.22) Std6 #VALUE!
* Note: b/b0 =absorbance standard (or sample)/absorbance zero standard
STEP 6: POSITIVE CUT-OFF VALUE 1.00 In ppb or ppm, input

STEP 7: SAMPLE DILUTION FACTOR 1.00 Check manual for input
Linear Regression
Standard Curve Parameters
Slope #VALUE!
Intercept #VALUE!
Correl #VALUE!
PLEASE DOUBLE CHECK:

-- Solvent blank sample (Yes or No) checked
-- Standard concentration values checked (from low to high)
-- Positive Cut-off Value checked
-- Sample Dilution Factor checked

STEP 8: TEST SUMMARY
Sample Name OD-1 B/B0 Results Pass/Fail Note Test Kit Name: 1024-05 - FLUOROQUINOLONE
Sample1 Sample1 #VALUE! #VALUE! NA Kit Lot #: 0
Sample2 Sample2 #VALUE! #VALUE! #VALUE! Sample Type: 0
Sample3 Sample3 #VALUE! #VALUE! #VALUE! Check in Time: 0
Sample4 Sample4 #VALUE! #VALUE! #VALUE! Detection Time: 0
Sample5 Sample5 #VALUE! #VALUE! #VALUE! Test Technician: 0
Sample6 Sample6 #VALUE! #VALUE! #VALUE! QA Technician: 0
Sample8 Sample8 #VALUE! #VALUE! #VALUE! OTHER NOTES
Sample9 Sample9 #VALUE! #VALUE! #VALUE! 0
Sample17 Sample17 #VALUE! #VALUE! #VALUE! Standard Curve
100%
Sample20
Relative Absorbance
60%
Sample25
20%
Sample31 Sample31 #VALUE! #VALUE! #VALUE! 0.0 0.1 1.0 10.0 100.0
Sample32 Sample32 #VALUE! #VALUE! #VALUE! Standard Concentration (ppb)


Please be careful when you change the parameters on this page
Ractopamine Clenbuterol Diazepam Sulfamethazine Diethylstilbestrol Chlorampheni Streptomycin

1 2
1 Yes
2 No
Food & Feed Safety ELISA Kits Standards
34 28 2 3 4 5 6
1 100801 1008-01 - RACTOPAMINE 0.50 1.00 5.00 10.00 25.00
2 100801 1008-01B - RACTOPAMINE 0.50 1.00 5.00 10.00 25.00
3 100803 1008-03 - RACTOPAMINE 0.04 0.10 0.25 0.60 1.50
4 100803 1008-03B - RACTOPAMINE 0.04 0.10 0.25 0.60 1.50
5 100805 1008-05 - RACTOPAMINE 0.05 0.20 0.80 3.20 9.60
6 100805 1008-05B - RACTOPAMINE 0.05 0.20 0.80 3.20 9.60
7 100901 1009-01 - BETA-AGONIST 0.05 0.15 0.50 2.00 4.50
8 100902-03 1009-02&03 - BETA-AGONIST 0.05 0.15 0.50 1.50 4.50
9 101001 1010-01 - DIAZEPAM 0.03 0.05 0.10 0.50 1.00
10 101102 1011-02 - SULFAMETHAZINE 0.25 0.50 1.00 5.00 10.00
11 101201 1012-01 - DIETHYLSTIBESTROL 0.15 0.50 1.50 4.50 9.00
12 101301-02-03
1013-01, 02 & 03 - CHLORAMPHENICOL (CAP) 0.050 0.15 0.50 1.50 4.50
13 101304-05 1013-04, 05 - CHLORAMPHENICOL (CAP) 0.02 0.05 0.15 0.50 1.50
14 101401 1014-01 - STREPTOMYCIN 0.50 1.00 2.50 5.00 10.00
15 101502 1015-02 - FURAZOLIDONE (AOZ) 0.05 0.15 0.50 1.50 4.50
17 101503 1015-03D - FURAZOLIDONE (AOZ) 0.03 0.09 0.18 0.54 1.62
18 101604 1016-04E - TETRACYCLINE 0.050 0.150 0.400 0.800 1.600
19 101701 1017-01 - ENROFLOXACIN 0.100 0.250 0.500 1.000 5.000
20 1017-02 1017-02A - ENROFLOXACIN 0.05 0.20 0.80 3.20 9.60
21 101703 1017-03, 03A - ENROFLOXACIN 0.35 1.00 2.50 7.50 22.50
22 101703B 1017-03B - ENROFLOXACIN 0.15 0.50 1.50 4.50 13.50
23 101704 1017-04 - ENROFLOXACIN 0.15 0.10 0.20 0.40 0.80
24 101901-02-03
1019-01, 02 & 03 - MALACHITE GREEN/LMG 0.500 1.50 5.00 10.00 50.00
25 101904-05-06
1019-04, 05 & 06 - MALACHITE GREEN/LMG 0.05 0.15 0.50 1.50 4.50
26 101907 1019-07 - MALACHITE GREEN/LMG 0.05 0.15 0.50 1.50 4.50
27 102001-02 1020-01 & 02 - FURALTADONE (AMOZ) 0.05 0.20 0.80 3.20 9.60
28 1020-03 1020-03 - FURALTADONE (AMOZ) 0.03 0.10 0.40 1.60 6.40
29 102101-03 1021-01&03 - CLENBUTEROL 0.05 0.15 0.50 1.50 4.50
30 102201 1022-01 - SALBUTAMOL 0.05 0.15 0.50 1.50 4.50
31 102401 1024-01 - FLUOROQUINOLONE 0.10 0.25 0.50 1.00 5.00
32 102401 1024-01B1 - FLUOROQUINOLONE 0.05 0.20 0.80 3.20 9.60
36 102502 1025-02 - CIMATEROL 0.250 1.00 5.00 20.00 50.00
37 102601 1026-01 - TYLOSIN 1.000 2.50 5.00 10.00 25.00
38 102701 1027-01 - GENTAMICIN 0.25 0.75 1.50 3.00 15.00

39 102702 1027-02 - GENTAMICIN 1.00 2.00 4.00 12.00 30.00
40 102901-02 1029-01 & 02 - CRYSTAL VIOLET/LCV 0.05 0.15 0.50 1.50 4.50
41 103002 1030-02 - TOTAL AFLATOXIN 0.05 0.10 0.20 0.40 0.80
42 103003 1030-03A - TOTAL AFLATOXIN 0.250 0.500 1.000 2.000 5.000
43 103004 1030-04 - TOTAL AFLATOXIN 0.100 0.300 1.000 3.000
44 103005 1030-05 - TOTAL AFLATOXIN 0.02 0.05 0.15 0.50 1.50

45 103102 1031-02 - ZILPATEROL 0.02 0.05 0.10 0.20 0.50
46 103103 1031-03 - ZILPATEROL 0.02 0.05 0.10 0.20 0.50
47 103104 1031-04 - ZILPATEROL 0.03 0.10 0.30 0.90 2.70
48 103302 1033-02 - SULFADIAZINE 1.000 2.000 5.000 10.000 25.000
49 103402 1034-02 - SAXITOXIN (PSP) 0.05 0.15 0.50 1.50 4.50
50 103501 1035-01 - ZEARALENONE 0.10 0.25 0.50 1.50 4.50
51 103503 1035-03 - ZEARALENONE 0.25 0.50 1.00 2.00 4.00
52 103602 1036-02 - OCHRATOXIN A 0.02 0.03 0.06 0.12 0.24
53 103603 1036-03 - OCHRATOXIN A 0.20 0.40 0.60 0.80 1.00
54 103701 1037-01 - T2 1.00 5.00 10.00 25.00 50.00
55 103703 1037-03 - T2 1.00 5.00 10.00 25.00 50.00
56 103802 1038-02 - FUMONISIN 1.00 5.00 10.00 25.00 50.00
57 103804 1038-04 - FUMONISIN 2.00 5.00 10.00 25.00 50.00
59 105301 1053-01 - STREPTOMYCIN (Honey) 0.50 1.00 2.50 5.00 10.00
60 105401 1054-01 - SULFAMETHOZAZOLE 0.500 1.500 5.00 15.00 50.00
61 105501 1055-01 - AFLATOXIN B1 0.05 0.10 0.20 0.40 0.80
62 105503 1055-03 - AFLATOXIN B1 0.50 1.00 2.00 5.00 10.00
63 105503 1055-03A - AFLATOXIN B1 0.13 0.50 2.00 8.00 32.00

64 105601 1056-01 - SULFONAMIDE 0.50 1.50 5.00 15.00 50.00
65 105701 1057-01 - SULFAQUINOXALINE (SQX) 1.000 2.50 5.00 15.00 50.00
66 105801 1058-01 - NORFLOXACIN 0.100 0.25 0.60 1.50 5.00
67 105901 1059-01 - FLUMQUINE 1.00 5.00 10.00 25.00 75.00

68 105902 1059-02 - FLUMQUINE 1.500 5.00 10.00 25.00 50.00
69 105903 1059-03 - FLUMQUINE 0.20 0.60 1.80 5.40 16.20
70 106001 1060-01 - AFLATOXIN M1 0.05 0.15 0.50 1.50 5.00
71 106002 1060-02 - AFLATOXIN M1 0.05 0.15 0.30 0.90 2.70
72 106004 1060-04 - AFLATOXIN M1 0.03 0.10 0.40 1.60 6.40
73 106005 1060-05 - AFLATOXIN M1 0.01 0.02 0.03 0.09 0.27

74 106005 1060-05A - AFLATOXIN M1 0.00 0.01 0.03 0.05 0.15
75 106006 1060-06 - AFLATOXIN M1 0.02 0.05 0.10 0.30 1.00
76 106401 1064-01 DON 2.50 5.00 10.00 25.00 50.00

77 106403 1064-03 DON 10.000 30.00 60.00 120.00 250.00
78 106501 1065-01 BETA LACTAM 0.080 0.20 0.40 0.80 1.20
79 106601 1066-01 Trifluralin 0.250 0.75 1.50 3.00 7.50
80 106801 1068-01 Ciprofloxacin 0.350 1.00 2.50 7.50 22.50
81 106901 1069-01 SEM 0.025 0.10 0.40 1.60 6.40
82 106902 1069-02 SEM 0.050 0.20 0.80 3.20 9.60

83 107001 1070-01 AHD 0.025 0.10 0.25 0.75 2.50
84 107002 1070-02 AHD 0.025 0.10 0.40 1.60 6.40
85 107101 1071-01 Zeranol 0.100 0.25 0.75 2.25 6.75
86 107301 1073-01 Avermectins 0.500 1.00 2.00 4.00 8.00
87 107302 1073-02 Avermectins 0.150 0.50 1.00 2.00 4.00
88 107402 1074-02 Methyltestosterone 0.100 0.50 2.50 10.00 50.00
89 107701 1077-01 Melamine 5.000 20.00 50.00 100.00 200.00
90 107703 1077-03 Melamine 1.000 5.00 15.00 45.00 135.00
91 107801 1078101 Terbutaline 0.050 0.15 0.50 1.50 4.50
92 107901 1079-01 Mabuterol 0.050 0.15 0.50 1.50 4.50
93 108001 1080-01 Testosterone 0.050 0.15 0.45 1.35 4.05
94 108101 1081-01 Oxytetracycline 0.150 0.38 0.75 1.50 4.50
95 108201 1082-01 Chlortetracycline 0.050 0.10 0.20 0.40 0.80
96 108301 1083-01 Doxycycline 0.150 0.38 0.75 1.50 4.50
97 108401 1084-01 Ceftiofur 2.000 8.00 16.00 32.00 64.00
98 108501 1085-01 Boldenone 0.050 0.15 0.50 1.50 4.50
99 108601 1086-01 Nandrolone 0.015 0.05 0.15 0.50 1.50
100 109002 1090-02 BaP 0.250 0.75 1.50 2.50 5.00
101 109301 1093-01 Erythromycin 0.500 1.00 2.00 4.00 8.00
102 109401 1094-01 Neomycin 1.000 2.50 5.00 10.00 25.00
103 109402 1094-02A Neomycin 1.500 4.50 13.50 27.00 81.00
104 109501 1095-01 Colistin 0.500 1.50 5.00 15.00 50.00
105 109601 1096-01 Cyproheptadine 0.015 0.05 0.15 0.50 1.50
106 109602 1096-02 Cyproheptadine 0.030 0.15 0.30 0.90 2.70
107 109701 1097-01 Dimetridazole 0.500 1.50 3.00 6.00 12.00
108 109801 1098-01 Carbendazim 0.500 1.50 3.00 6.00 12.00
109 110401 1104-01 Doramectin 1.500 5.00 15.00 30.00 60.00
110 110501 1105-01 Chlorpromazine 0.040 0.10 0.25 0.50 1.50
111 110601 1106-01 Reserpine 0.250 0.50 2.50 5.00 10.00
112 110701 1107-01 Florfenicol 0.150 1.00 5.00 25.00 100.00

113 110901 1109-01 Ethoxyquin 0.100 0.30 0.90 2.25 4.50
114 111001 1110-01 Okadaic Acid (DSP) 0.300 0.90 2.70 5.40 16.20
115 111201 1112-01 Dexamethasone (Milk) 0.015 0.05 0.15 0.50 1.50
116 111201 1112-01 Dexamethasone 0.015 0.05 0.10 0.20 0.50
117 111202 1112-02 Dexamethasone 0.015 0.05 0.15 0.50 1.50
118 111203 1112-03 Dexamethasone 0.050 0.15 0.50 1.50 4.50
119 111301 1113-01 Ofloxacin 0.100 0.25 0.50 1.00 5.00
120 111401 1114-01 Amoxicillin 0.200 0.40 0.80 1.60 3.20
121 111701 1117-01 Domoic Acid (ASP) 0.063 0.13 0.25 0.50 1.50
122 112001 1120-01 Tilmicosin 0.250 1.00 2.50 5.00 10.00
123 112101 1121-01 Benzalkonium Chloride (BKC) 0.200 0.60 1.80 5.40 16.20
124 112102 1121-02 Benzalkonium Chloride (BKC) 2.500 7.50 25.00 75.00 250.00
125 112201 1122-01 Cefoperazone 0.250 0.50 1.00 2.00 4.00
126 112301 1123-01 Amantadine 0.500 1.50 4.50 13.50 27.00
127 112303 1123-03 Amantadine 0.300 0.90 2.70 8.10 16.20
128 120602 1206-02 Lincomycin 0.100 0.25 0.60 1.50 3.75
129 120701 1207-01 Pirlimycin 5.000 50.00 250.00 750.00 2250.00
130 120801 1208-01 Sarafloxacin 0.050 0.20 0.80 3.20 12.80
131 121001 1210-01 Ampicillin 0.080 0.20 0.40 0.80 1.20
132 109901 1099-01 Trimethoprim 0.015 0.04 0.12 0.36 1.08

Analysis Model
1 1
2 Linear Regression
Cubic Spline
AOZ Tetracycline Enrofloxacin Malachite Green Salbutamol
Furaltadone (AMOZ)
1 1 2
Std. Conc. (ppb)

6 5 4 9 6
Std1 Std2 Std3 Std4 Std5 Std6
1 0 0.010 0.030 0.060 0.120 0.240
2 0 0.015 0.040 0.075 0.150 0.250
3 0 0.020 0.050 0.080 0.160 0.320
4 0 0.025 0.075 0.100 0.300 0.500
5 0 0.050 0.100 0.150 0.400 0.600
6 0 0.100 0.150 0.200 0.500 0.800
7 0 0.150 0.200 0.300 1.000 0.900
8 0 0.250 0.250 0.400 1.500 1.000
9 0 0.450 0.500 0.450 1.600 1.500
10 0 0.500 0.750 0.500 2.000 4.500

11 0 1.000 1.000 0.800 2.500 5.000
13 0 2.000 2.000 1.500 3.200 9.000

14 0 2.500 2.500 2.500 4.500 9.600
15 0 5.000 5.000 5.000 5.000 10.000
16 0 10.000 10.000 10.000 10.000 15.000

17 0 15.000 15.000 15.000 20.000 25.000
18 0 25.000 25.000 25.000 25.000 50.000

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BIOO MaxSignal® ELISA Analysis Program in Excel (with BIOO Specified Standards)

STEP 1: TEST NOTES OTHER NOTES

Test Kit Name: 1020-03 - FURALTADONE (AMOZ)

STEP 2: PLATE LAYOUT DIAGRAM

BIOO MaxSignalTM ELISA Detection Analysis . 1

STEP 4: PLEASE DEFINE SAMPLE 1 - IS IT A SOLVENT BLANK SAMPLE? Yes

STEP 5: STANDARDS CONCENTRATION VALUES

STEP 6: POSITIVE CUT-OFF VALUE 0.05 In ppb or ppm, input

PLEASE DOUBLE CHECK:

BIOO MaxSignalTM ELISA Detection Analysis . 2

Sample1 2.478 0.962 0.0000 NA Kit Lot #: 102003112916$64975146799

BIOO MaxSignalTM ELISA Detection Analysis . 3

BIOO MaxSignalTM ELISA Detection Analysis . 4

STEP 1: TEST NOTES OTHER NOTES

Test Kit Name:

STEP 2: PLATE LAYOUT DIAGRAM

BIOO MaxSignalTM ELISA Detection Analysis . 5

STEP 4: PLEASE DEFINE SAMPLE 1 - IS IT A SOLVENT BLANK SAMPLE? Yes

STEP 5: STANDARDS CONCENTRATION VALUES

STEP 6: POSITIVE CUT-OFF VALUE 1.00 In ppb or ppm, input

PLEASE DOUBLE CHECK:

BIOO MaxSignalTM ELISA Detection Analysis . 6

BIOO MaxSignalTM ELISA Detection Analysis . 7

BIOO MaxSignalTM ELISA Detection Analysis . 8

Ractopamine Clenbuterol Diazepam Sulfamethazine Diethylstilbestrol Chlorampheni Streptomycin

36 102502 1025-02 - CIMATEROL 0.250 1.00 5.00 20.00 50.00

37 102601 1026-01 - TYLOSIN 1.000 2.50 5.00 10.00 25.00

38 102701 1027-01 - GENTAMICIN 0.25 0.75 1.50 3.00 15.00

41 103002 1030-02 - TOTAL AFLATOXIN 0.05 0.10 0.20 0.40 0.80

42 103003 1030-03A - TOTAL AFLATOXIN 0.250 0.500 1.000 2.000 5.000

43 103004 1030-04 - TOTAL AFLATOXIN 0.100 0.300 1.000 3.000

44 103005 1030-05 - TOTAL AFLATOXIN 0.02 0.05 0.15 0.50 1.50

63 105503 1055-03A - AFLATOXIN B1 0.13 0.50 2.00 8.00 32.00

66 105801 1058-01 - NORFLOXACIN 0.100 0.25 0.60 1.50 5.00

67 105901 1059-01 - FLUMQUINE 1.00 5.00 10.00 25.00 75.00

70 106001 1060-01 - AFLATOXIN M1 0.05 0.15 0.50 1.50 5.00

71 106002 1060-02 - AFLATOXIN M1 0.05 0.15 0.30 0.90 2.70

72 106004 1060-04 - AFLATOXIN M1 0.03 0.10 0.40 1.60 6.40

73 106005 1060-05 - AFLATOXIN M1 0.01 0.02 0.03 0.09 0.27

76 106401 1064-01 DON 2.50 5.00 10.00 25.00 50.00

78 106501 1065-01 BETA LACTAM 0.080 0.20 0.40 0.80 1.20

79 106601 1066-01 Trifluralin 0.250 0.75 1.50 3.00 7.50

80 106801 1068-01 Ciprofloxacin 0.350 1.00 2.50 7.50 22.50

81 106901 1069-01 SEM 0.025 0.10 0.40 1.60 6.40

82 106902 1069-02 SEM 0.050 0.20 0.80 3.20 9.60

84 107002 1070-02 AHD 0.025 0.10 0.40 1.60 6.40

85 107101 1071-01 Zeranol 0.100 0.25 0.75 2.25 6.75

86 107301 1073-01 Avermectins 0.500 1.00 2.00 4.00 8.00

87 107302 1073-02 Avermectins 0.150 0.50 1.00 2.00 4.00

88 107402 1074-02 Methyltestosterone 0.100 0.50 2.50 10.00 50.00

89 107701 1077-01 Melamine 5.000 20.00 50.00 100.00 200.00

90 107703 1077-03 Melamine 1.000 5.00 15.00 45.00 135.00

91 107801 1078101 Terbutaline 0.050 0.15 0.50 1.50 4.50

92 107901 1079-01 Mabuterol 0.050 0.15 0.50 1.50 4.50

93 108001 1080-01 Testosterone 0.050 0.15 0.45 1.35 4.05

94 108101 1081-01 Oxytetracycline 0.150 0.38 0.75 1.50 4.50

95 108201 1082-01 Chlortetracycline 0.050 0.10 0.20 0.40 0.80

96 108301 1083-01 Doxycycline 0.150 0.38 0.75 1.50 4.50

97 108401 1084-01 Ceftiofur 2.000 8.00 16.00 32.00 64.00

98 108501 1085-01 Boldenone 0.050 0.15 0.50 1.50 4.50

99 108601 1086-01 Nandrolone 0.015 0.05 0.15 0.50 1.50

100 109002 1090-02 BaP 0.250 0.75 1.50 2.50 5.00

101 109301 1093-01 Erythromycin 0.500 1.00 2.00 4.00 8.00