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Enzimatic Kinetic Acosta Y. - Altamar R. - Vargas S.
Enzimatic Kinetic Acosta Y. - Altamar R. - Vargas S.
FACULTAD DE INGENIERIA
INGENIERIA QUÍMICA
ENZIMATIC KINETIC
Authors: Yourladys Acosta – Roberto Altamar - Stephanie Vargas
Bioprocesses
Delivery date: 07/03/2022
Parameter value
k1 20
k-1 1
k2 10
S0 10
[E]0 2
𝜕𝑆
= 𝑘−1 [𝐸𝑆] − 𝑘−1 [𝑆][𝐸]
𝜕𝑡
𝜕𝐸
= (𝑘2 + 𝑘−1 )[𝐸𝑆] − 𝑘1 [𝑆][𝐸]
𝜕𝑡
𝜕𝐸𝑆
= 𝑘1 [𝑆][𝐸] − [𝐸𝑆](𝑘2 + 𝑘−1 )
𝜕𝑡
𝜕𝑃
= 𝑟2 = 𝑘2 = [𝐸𝑆] = 𝜈
𝜕𝑡
SOLUTION
First you must have the desolve library installed, then enter the equations to be solved,
the parameters, the time range where the equations will be evaluated and then use the
ode function to solve the equations.
UNIVERSIDAD DEL ATLÁNTICO
FACULTAD DE INGENIERIA
INGENIERIA QUÍMICA
Then the concentrations of the substrate, enzyme, enzyme-substrate and product are
plotted with respect to time.
SOLUTION
First we add the data that give us the concentration and initial velocity of the lactose, then
we plot the graph the profile of initial reaction velocity vs substrate concentration.
UNIVERSIDAD DEL ATLÁNTICO
FACULTAD DE INGENIERIA
INGENIERIA QUÍMICA
Figure 3. Lineweaver-Burk
Lineweaver Eady – Hofstee
For the method we plot v vs v/[S], from the graphs we obtain the parameters to find km and
vm. Taking into account that the slope corresponds to -km and the intercept to vm.
UNIVERSIDAD DEL ATLÁNTICO
FACULTAD DE INGENIERIA
INGENIERIA QUÍMICA
Solution
Initially find the inverse of the substrate concentration and the inverse of the rate of product
formation of the three types of inhibitor and no inhibitor and plot this values
Regression is calculated to find the values of the slope and the intercept, this is done
without inhibitor type and inhibitor A, B and C
UNIVERSIDAD DEL ATLÁNTICO
FACULTAD DE INGENIERIA
INGENIERIA QUÍMICA
Being the first coeficcientthe values of intercept and second one the values of slope
After that, we calculated the kinetic parameters of the different types of reversible
inhibition
Competitive Inhibition
𝑣𝑚 𝑆
𝑣=
𝐼
𝐾𝑑 (1 + 𝐾 ) + 𝑆
𝑖
𝐼
𝐾𝑑 (1 + ) = 𝐾𝑑𝑎𝑝𝑝
𝐾𝑖
𝑣𝑚 𝑆
𝑣=
𝐾𝑑𝑎𝑝𝑝 + 𝑆
UNIVERSIDAD DEL ATLÁNTICO
FACULTAD DE INGENIERIA
INGENIERIA QUÍMICA
Uncompetitive Inhibition
𝑣𝑚𝑎𝑝𝑝 [𝑆]
𝑣= ′
𝐾𝑑𝑎𝑝𝑝 + [𝑆]
′
𝐾𝑑
𝐾𝑑𝑎𝑝𝑝 =
𝐼
(1 + )
𝐾𝑖′
𝑣𝑚
𝑣𝑚𝑎𝑝 =
𝐼
1+
𝐾𝑖′
UNIVERSIDAD DEL ATLÁNTICO
FACULTAD DE INGENIERIA
INGENIERIA QUÍMICA
Non-Competitive Inhibition
𝑣𝑚𝑎𝑝𝑝 [𝑆]
𝑣=
𝐾𝑑 + [𝑆]
𝐾𝑖 = 𝐾𝑖′
𝑣𝑚
𝑣𝑚𝑎𝑝𝑝 =
𝐼
(1 + 𝐾 )
𝑖