XII BIOLOGY ASSIGNMENT BIOTECHNOLOGY

BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

1) Name and describe the technique that helps in separating the DNA fragments formed by the
use of restriction endonuclease.
2) A) Draw a schematic sketch of pBR322 plasmid and label the following in it
 Any two restriction sites.
 Ori and rop
 An antibiotic resistant gene.
B) Why are genes encoding resistance of antibiotics considered useful selectable markers for
E.coli cloning vector?
3) Name the vector for-A ) Bacteria B) Plant cell C) Animal cell
4) How is insertional inactivation of an enzyme used as a selectable marker to differentiate
recombinants from non-recombinants?
5) How growth of bacteriophage restrict in E.coli
6) Any recombinant DNA with a desired gene is required in billion copoies for commercial use.
How is the amplification done? Explain.
7) What are bioreactors? List five growth conditions that a bioreactor provides for obtaining the
desired product.

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Biotechnological Applications in Agriculture and Medicine

1) Name the cry genes that control cotton bollworm and com borer respectively.
2) How does silencing of specific mRNA in RNA interference prevent parasitic infection?
3) What is ADA deficiency? Describe three methods to cure it.
4) How did Eli Lilly synthesise the human insulin? Mention one difference between this insulin
and the one produced by the human pancreas.
5) List the three molecular diagnostic techniques that help to detect pathogens from suspected
patients, Mention one advantage of these techniques over conventional methods.
6) Define the fallowing term
‘Rosie’
Biopiracy
GEAC

PREPARED BY –SUDHEER VERMA (ACADEMIC CO-ORDINATOR)

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