General objective

 Screening of Black Cumin (Nigella sativa) Varieties for Drought Tolerance

and Response Analysis Using Morphological, Physiological Traits, and
Proteomic, Approach

Specific objectives
1. To screen drought resistant varieties
2. To analyze mechanism of drought response using morphological traits
3. To analyze mechanism of drought response using physiological traits
4. To identify drought resistance proteins using proteomic approach

Physiological traits Proteomic/molecular traits Morphological traits

Leaf temperature Protein Extraction plant size
Stomata closure Quantification Leaf area
Cell wall integrity Gel Electrophoresis Root extension
Oxidative stress Gel Staining Roots dry weight,
Photosynthesis Image Analysis density, and length
Turgor pressure Protein Classification Early maturity
CO2 concentration Mass Spectrometry Yield
Growth rate iTRAQ analysis Leaf extension
Stomata conductance QTL mapping Leaf size
Relative water content Leaf number
Membrane integrity Leaf longevity
Transpiration Root-to-shoot ratio
Water use efficiency
Transpiration efficiency
Total biomass
Alternative oxidase (AOX)

In vitro screening of chili (Capsicum frutenscens)/black pepper/korarima varieties for drought

stress tolerance and response analysis using physiological, proteomic, and biochemical methods

5. To screen drought resistant variety

6. To analyze mechanism of drought response using physiological characters
7. To analyze mechanism of drought response using proteomic methods
8. To analyze mechanism of drought response using biochemical methods
6.11.9. Drought Frequent drought also another factor that affects the growth and yield of red
pepper in the quality and quantity of the product. Hence, if the smallholder producers are unable
to get other supplementary inputs, i.e., irrigation, the production and productivity are obviously
at risk as well (Wubalem, 2019).

Physiological traits associated with drought, such as

1. Carbon isotope discrimination,
2. Leaf temperature and
3. stomata conductance (Khan et al. 2010),
4. spectral indices structure‐insensitive pigment index and
5. normalized pheophytinization index (Maalouf, Nachit, Ghanem, & Singh, 2015)

Genome Wide Identification and Characterization of Polygalacturonase Gene in

Korarima (Aframomum corrorima (Braun) Jansen)

Identification and characterization of polygalacturonase gene family involved in

banana fruit softening

from and after a genome-wide investigation of the polygalacturonase gene family

from the banana genome.

Genome-wide analysis of polygalacturonase

gene family from banana genome and
identification of the member involved in
banana fruit softening