Mansoura University Post graduate studies exam Faculty of Veterinary Medicine ! Diploma of Clinical Chemistry Department of Biochemistry ess Molecular biology Course, 14 August 2020 and Chemistry of Nutrition we Time allowed :1 hours, Total Marks:30 (Oral Examination) Question (1): Which of the following statement is true (T) or false (F). (35 marks). 41) Uracil is a nitrogenous base derived from purines. - 2) RNA molecule contains uracil instead of thiamine 3) Mature RNA contains Intron in its structure 4) FRNA consists of large and smail subunits. ’5) The most energy consuming process in molecular biology is translation 6) histones are tetrameric proteins 7) Histones are rich in acidic amino acids 8) Okazaki! fragments are formed in leading strand of DNA 9) Topoisomerase is required for removal of super coils of DNA 10) RNA polymerase is consisted of 6 subunits 11) The DNA template for transcription runs from 5 to3 direction 12) The poty adenine tail of mRNA presents in 3'and 43) Repressor protein binds with operator genes for induction of transcription +44) The main difference between nucleotide and nucleoside is the absence of phosphoric acid . 15) Replication on the (agging strand is interrupted 46) RHO factor is required for termination of transcription 417) The most dangerous type of mutation is point mutation 18) Silent mutation is occurred mainly ifthe change in the third base of the codon 19) The termination codons are UAA, UGA, UGG 20) The circular ONA that is required for cloning is plasmid 21) The type of restriction enzymes that is used in cloning should produce blunt end, 22) transformation of clones is required for expression of inserted genes 2) There are 3 types of restriction enzymes 24) Easiern blotting is required for detecting proteins 26) Gel electrophoresis is required for visualizing of PCR products 3¢ 26) Recombinant DNA technology is required for production of vaccines . 27) The transfer of RNA to cDNA required reverse polymerase enzyme 28) The first step in cloning technique is ONA extraction 28) PCR is conducted for ampitication of desired genes 30} For quantification of certain gene, PT-PCR is conducted 31) SDS-PAGE Is conducted for protein electrophoresis process » + 32) Isolation of DNA requires high sall concentration for the movement of DNA from nucleus to the butter solution 33) Final elongation step acts as proof-reading step simulating DNA replication 34) Before, PCR, northern biot is used for detection of foreign DNA 35) Extraction of RNA is occurred in alkaline medium vlF Uésle Wet {Ff fis] ry ger fF peel er Pe | : 27 fe lf Peel pels feel pat] Ff 32 | 3 ]F Baler Fgts| 7 Pes |r yes 7 p33 TF a |r g7ir re Cae oe Ee EX Ste hte LE Tyee (r se Pee TF fs TePractical Examination Question (2) Select the one lettered answer in each case of the following (15 marks) | |) Answer the following questions on the following gel electrophoresis 41) The sequencing technique is. }) Sanger method. ‘b) Chemical cleavage method } Automatic sequencing method ¢) None of these 2) The deduced sequence WGACTATC. b)CTATCAG —¢) Both _d) None 3) For chemical cleavage method... Is used to cut fragment 2) Pepsin b) Papain N)Piperacine a) Trypsin 4) In maxam and gilbert... is used for depurination a) Dimethy! sulfate Formic acid c) phenyl hydrazine d} Nod a) deoxynucleotide W) di deoxynucleotide ¢) rbonuclectide d) None 6) The sequencing technique that is used before PCR discovery is 5) In sanger sequencing method,..... is required ! a) Sanger’ MMaxam and Gibert c) Automated sequencing d) None} L JL Answer the following question after reading the following sequence AGT CAG TAG TCA GTC AGT CAG TAG TA 7) Tm of this sequence is a) 104 W74 c)26 a)30 8) The optimum annealing temperatures °c 59 B/S c)21 d)25 9) GC% Is W423 b)4a4 Qa42 ara 410) The optimum nucleotides bases for a proper primer for gene expression ranges from a)5-10 b)10-15 18-23 d)30.35 414) The optimum Tm value of primer for conducting PCR Is. °c W55-85 —b)40-45¢) 70-75" 6) 30-35 12) The optimum GC% for primer for conduction POR is a) 25-30 b)35-45 WL 45-65 4) 55.65 43) The following primer is not suitable for PCR bec: a)Tmis high b)GC%islow )Bothaand’ —d) Neither nor b 14) The optimum temperature for denaturation of DNA template during PCRis °c ajon 95 d)85 d) 99 18) The bonds between guanine and cytosine Is @)Single b) double ¥) tripled) Quaderate Good Luck