LC 300 HPLC and UHPLC Systems P R O D U C T N O T E

Liquid Chromatography

Key Features:
• Enhanced ease-of-use and
operational simplicity
• High visibility color display for immediate
assessment of system status
• Integrated solvent containment and column
oven for reduced system complexity
• Precise, repeatable solvent delivery
• Outstanding injection reproducibility – even at
low volumes
• Automated sample dilution and derivatization
• Wide range of detectors: PDA,
Multi-Wavelength UV/Vis,
Single wavelength UV/Vis, FL and RI
• Robust and easy to maintain system
• Fully controlled by PerkinElmer
Simplicity™Chrom and other leading
CDS platforms
• Instrument control and data acquisition via
Ethernet communication

Increasing Your Productivity

Takes More Than Just Superior Introduction
Laboratories are continuously
Analytical Performance – faced with the daunting task

It Takes PerkinElmer’s LC 300 of increasing their productivity,

while facing challenges with
budgets and staffing levels.
Meeting these challenges takes more than just a high performing liquid chromatography
system – it requires a system from a partner who understands your challenges, and
designs solutions to solve them.
The PerkinElmer LC 300 liquid chromatography systems are the result of listening to
user needs, and paying careful attention to every detail in the resulting design. The LC
300 systems are the ideal solution for labs faced with high throughput requirements or
challenging chromatographic separations.
Whatever the application, the PerkinElmer LC 300 system’s performance and enhanced
user experience will ensure your lab’s success, whether that need be for pharmaceutical,
food, consumer products or specialty chemicals.
 LC 300 AUTOSAMPLERS

Key Features Include:

• 10,000 psi (690 bar) or 18,000 psi
(1240 bar) autosampler
• Patented injection valve design
• High visibility color LCD display showing complete
LC system status, current queue and sample progress
• Integrated column temperature compartment settable
from ambient +5 °C to 60 °C
• Precise, uniform sample tray temperature control from
4 °C to 40 °C (Peltier option)
• Wide variety of vial and microtiter tray formats
• Sample injection volumes as low as 1 μL, at pressures
up to 18,000 psi
• Three injection modes: full loop, partial fill and
The most important part of your lab’s workflow is the μL-pickup for maximum flexibility
handling of your samples by the autosampler. Ensuring that • Loads sample in only eight seconds (in partial fill mode)
samples are not subject to degradation or contamination, • Integrated solvent and waste tubing management design
that required dilution or derivatization is done in an
automated and fully documented manner, and that the
samples themselves are injected precisely are all paramount
The LC 300 also features an integrated column oven
to achieving reproducible and accurate analytical results.
with preheater to support a single column up to 150 mm in
It’s also critical that the flowpath and needle be thoroughly
length. This capability eliminates the need for another module
cleaned to avoid cross-contamination, and that the system
in the system stack, and reduces the length of the required
be ready for the next injection quickly, to ensure throughput
interconnect tubing for optimal peak shape and resolution.
goals are achieved. The LC 300 family of autosamplers have
been engineered with the most advanced fluidics technology In addition to the key technical features of the LC 300
available, with close attention paid to ensuring precision and Autosampler, an integrated, full color LCD display provides users
high reproducibility for superior analytical performance. with critical operating feedback. Unlike many other systems, the
LC 300 status display allows users to see operational conditions in
From the moment your samples are placed in the LC 300,
real time, right on the system. The high visibility display includes
the system works to protect them from degradation or
color coded information on pump composition and pressure,
precipitation. While many autosamplers claim to offer some
detector wavelength(s), sample and column temperature,
form of cooling or heating of the sample tray, the consistency
current vial being analyzed, as well as the overall progress of the
of the temperature may not always be reliable. The LC 300
total sequence. The freedom and convenience of always having
Autosampler can be configured with optional Peltier cooling to
this information available at a glance eliminates the need to
provide uniform and precise cooling of all samples in the tray,
continuously check in on the controlling computer.
as well as the environment surrounding them. If automated
dilution or derivatization is required – or if preparation of
calibrants is desired – the LC 300 can perform these once
laborious tasks with no additional hardware or software. When
it’s time to perform an analysis, the LC 300’s advanced fluidics
design delivers outstanding injection-to-injection reproducibility,
even for very low injection volumes. The patented* injection
valve design makes the LC 300 ideal for analyses at extreme
pressures (up to 18,000 psi with the UHPLC configuration),
while an ultra-fast valve switching mechanism reduces
pressure and shock perturbations that would otherwise
negatively affect analytical performance and impact column
life. Performing fast and effective needle washing with a
choice of different wash solvents reduces the chances of
carryover and contaminant concerns.

*U.S. Patent No. 8,322,197 B2, European Patent No. 2196801

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 LC 300 PUMPS
Accuracy and precision aren’t just requirements for an
autosampler. In fact, accurate, precise and rugged formulation
and delivery of your mobile phase is critical to your results.
The LC 300 Pump family utilizes an advanced implementation
of a rugged linear drive to deliver ultra-precise gradient flows
up to 10,000 psi (HPLC configuration) or 18,000 psi (UHPLC
configuration). Each of the four pump heads is individually
motor-driven and controlled, generating maximum freedom
for precise flow control and pulsation reduction. Intelligent
flow control algorithms in the microprocessor create stable and
accurate solvent flows, no matter what solvents or flow rates you
choose. The LC 300 Pump incorporates truly automatic solvent
compressibility compensation (no need to enter factors in the
method), and is essentially self-cleaning via an active seal wash
system that ensures maximum life of the piston seals. Unlike
many other high pressure and ultra-high pressure solvent delivery
system, the LC 300 was engineered to allow for quick routine
maintenance to provide maximum uptime and lab productivity.
LC 300 FAMILY OF DETECTORS
High sensitivity detection is essential for most LC applications.
The PerkinElmer LC 300 system can be configured with either a
single detector, or multiple LC detection options to meet all of
your needs, even if they evolve or change in the future. Whether
its photodiode array (PDA), multi-wavelength UV/Vis (MWD),
single wavelength UV/Vis, fluorescence (FL) or refractive index
(RI) detection, the LC 300 family of detectors connect quickly
Key Features Include:
• 10,000 psi (690 bar) or 18,000 psi (1240 bar) solvent
delivery system
• Select from up to four solvents with binary solvent delivery
• Integrated vacuum degassing technology
• Accurate and precise flows via advanced linear drives
and intelligent flow control algorithms
• Automatic solvent compressibility compensation to
ensure accurate solvent blends
• Long seal life via a dedicated and automated piston
seal wash mechanism
• Specifically designed for quick and easy
routine maintenance
• Self-priming to speed up and simplify solvent change-
over and system start-up
• Patent pending pump head design prevents the
formation of air bubbles in the pump chamber
• Integrated solvent and waste tubing management design
A novel automatic priming design allows the system to achieve full
self-priming capabilities, automating the otherwise cumbersome
priming process utilized in many other systems. There’s no need to
manually help prime the pump with a syringe, as the LC 300 can
truly prime and purge all by itself in just a few minutes, freeing
you to prepare your samples or handle other important laboratory
tasks. Finally, the LC 300 incorporates built-in solvent degassing
technology, thus eliminating the need for a separate, external
degassing module.
Key Detector Range Features:
• Support for both HPLC and UHPLC applications
• Choice of five detectors: PDA*, MWD*, UV/Vis*, FL*
and RI
• Designed for quick and easy routine maintenance
• Integrated solvent and waste tubing management design
*Denotes detectors with wavelength programming capability.
and easily to the system via Ethernet communication to provide
robust control and secure data transfer. All detectors feature
an integrated solvent and waste tubing management system,
while the PDA, MWD and UV/Vis detectors also feature a built-
in solvent organizer to reduce the number of modules in the LC
stack, thus reducing asset management complexity.
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 LC 300 PHOTODIODE ARRAY DETECTOR (PDA)
The LC 300 PDA detector is ideal for laboratories balancing
high sample throughput requirements with methods that
demand high levels of sensitivity. Additionally, the PDA
detector’s qualitative capabilities of spectral identification and
confirmation reduce risk and increase confidence in analysis.
The LC 300 PDA’s wide linear dynamic range (up to 2.0 AU
with less than 3% deviation) rivals or exceeds that of even
conventional UV detectors. Low baseline noise coupled
with high linear dynamic range provides for the ultimate in
analyte concentration tolerance. This allows detection from
very low concentrations (those previously lost in the noise)
to high concentrations, with no need to dilute samples. An
interchangeable, modular, novel patented* flow cell design
LC 300 MULTI-WAVELENGTH UV/VIS DETECTOR (MWD)
The LC 300 Multi-Wavelength UV/Vis detector (MWD) is
designed for laboratories that require more than one single
wavelength to be monitored, processed and stored per analysis.
In fact, the LC 300 MWD allows for the collection of up to eight
channels simultaneously at any desired wavelength or sampling
rate. The LC 300 MWD offers a wide linear dynamic range
(up to 2.0 AU, with less than 3% deviation), very low baseline
noise, and sampling rates up to 200 Hz. This allows detection
from very low to very high concentrations, with no need to
LC 300 UV/VIS DETECTOR
The LC 300 UV/Vis detector was designed to achieve a
high level of sensitivity and stability through innovative
engineering. The LC 300 UV/Vis features a proven dual-beam
optical design for ultimate stability and sensitivity. Utilizing an
optional tungsten lamp, the LC 300 UV/Vis can be configured
LC 300 FLUORESCENCE DETECTOR
The LC 300 Fluorescence detector enables the selective
detection of fluorescing compounds, with sensitivity that
can be up to 1,000 times greater than UV detection, which
is critical in many applications. The LC 300 Fluorescence
detector was designed to achieve a high level of sensitivity and
stability through innovative engineering, including low noise
electronics, an axially irradiated flow cell, and a dynamically
*US Patent Numbers US8797528, US8947654, and US9025142; Chinese Patent Number CN204945029; EU Patent Allowed EP13780024.9
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allows for one-motion installation and exchange, enabling greater
flexibility and ultimately higher productivity in your lab. To
further improve your sensitivity, an optional 50 mm flow cell
is available. The combination of sampling rates up to 200 Hz,
low optical dispersion, and the benefits of spectral identification
make the LC 300 PDA detector the perfect solution for almost
any application.
Patented light guided 50 mm flow cell.
dilute samples. Borrowing from the LC 300 PDA design, the
MWD also features an interchangeable modular flow cell that
allows for quick and easy one-motion installation and exchange,
enabling greater flexibility and higher productivity in your lab.
To further improve your sensitivity, an optional patented 50 mm
flow cell is available. To future-proof your investment should
your analysis needs change, you can upgrade your MWD to a
full-featured photodiode array detector with spectral analysis
capabilities.
for high sensitivity in the visible region – a sacrifice many
other detectors make when using a single, universal source
lamp design. The LC 300 UV/Vis can be optimized for any
application, and supports sample rates up to 100 Hz for
UHPLC application needs.
temperature-controlled cell body design. The LC 300
Fluorescence detector can simultaneously collect data at two
sets of wavelengths (excitation and emission) for additional
chromatographic confirmation. An integrated mercury
lamp is provided to check wavelength accuracy, ensuring
confidence in performance.
 LC 300 REFRACTIVE INDEX DETECTOR

The LC 300 Refractive Index (RI) detector enables the system mounted within a precisely temperature-controlled
detection of compounds that do not absorb in the UV/Vis housing, reducing the impact of even subtle changes in
range, and do not naturally, or cannot be made to fluoresce. ambient temperature. The LC 300 RI detector contains a
RI detection is commonly employed in the analysis of long-life LED light source for the ultimate in uptime and
carbohydrates and sweeteners. The LC 300 RI detector was productivity.
designed to achieve outstanding stability through an optical

LC 300 PELTIER COLUMN OVEN

While the LC 300 system incorporates an integrated

Key Features Include:
column oven within the autosampler, occasionally
the need to support multiple or long column formats • Accurate and precise temperature control
is required. In addition, some applications require a from 5 °C to 90 °C (75 °C when the
column temperature specifically at or below ambient for column selection valve is installed)
optimal results. The LC 300 Peltier Column Oven was • Integrated solvent preheater
designed as the ideal solution for all of these situations. • Temperature and vapor safety sensors
The LC 300 Peltier Column Oven delivers precise and with alarm
consistent temperature control, while offering a spacious • Large column compartment that can
multi-column compartment for quick and easy access. accommodate HPLC or UHPLC columns
An integrated solvent pre-heater mechanism that affects up to 30 cm in length
solvent temperature before it reaches the column is
• Optional column selection valve for
employed to ensure maximum stability and reproducibility
automated column switching of up to
from run-to-run. An optional column selection valve can
six columns
be installed at any time to support automated column
selection of up to six columns via the SimplicityChrom
chromatography data system (CDS).

LC 300 WASTE MANAGEMENT MODULE

The LC 300 Waste Management Module allows for switch allows for easy and efficient powering on and off
increased flexibility with the option to drain from the front of the entire system. A standalone solvent organizer is also
or the back of this module. An integrated power strip built available for the LC 300.
into the instrument streamlines operation, and reduces
external power outlet requirements. Further, a single power
Key Features Include:
• Drainage from front or back
• Built-in power strip
• Easily accessible single power switch for the entire system

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com

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