Problems (Chapter: UV-VIS spectroscopy)

(1) Aniline C6H5 NH2 ( max = 280 nm, = 1430 mol –1. cm-1.L). How many grams of aniline
should be used to make 200.0 mL of aniline with 40% transmittance (standard cuvette, b =
1cm).
(2) Determination of quinine in a sample solution:
25.00 mL of quinine sample solution is diluted to 50.00 mL and absorbance of this
solution is measured at  max = 384 nm, A = 0.426 ( cuvette, b = 1cm).
25.00 mL dd quinine sample solution + 10.00 mL of 20 ppm quinine, diluted to 50.00
mL and absorbance of this solution is measured at  max = 384 (cuvette, b = 1cm), A = 0.720.
Calculate concentration (ppm) of quinine in the original sample solution.
(3) Determination of Cu in a food sample by VIS spectroscopy: solid sample (5.000g) is
dissolved and Cu 2+ is reacted with dithizone to form a colored complex in chloroform. The final
volume of copper in chloroform is 50.0 mL. Absorbance of sample is measured at = 545 nm, b
= 1cm), A (sample) = 0.205. A standard solution containing 20 µg Cu 2+ is performed (the same
procedure with sample) in 25.00 mL volumetric flask. Absorbance of standard solution is 0.375.
Calculate concentration of Cu (ppm) in the original solid sample.
(4) Determination of iron by VIS spectroscopy: Iron (Fe3+) is reduced to Fe2+ and then Fe2+ is
reacted with 1-10 phenanthroline at pH 5 to form an orange-red solution. Absorbances of
standard and sample solutions are measured at 510 nm (pathlength b = 1cm).
Sample (7.720 g) is dissolved using a suitable method and diluted to 100.0 mL (solution A).
Take 10.00 mL of solution A, perform complex reaction in 100.0 mL–volumetric flask. Standard
solutions are prepared from a stock solution of 1.00 M x 10-3 M Fe 3+ in 50.0 mL–volumetric
flasks (Table 1). Absorbances of sample and standard solutions are reported in Table 1.
Calculate concentration of iron (ppm) in the original solid sample.
Table 1
Standards Samples
C0 C1 C2 C3 C4 C5 M0 M1
V (0.00100 M Fe3+), mL 0 1.00 2.00 3.00 4.00 5.00 - -
C (Fe3+), mol/L
A 0 0.221 0.432 0.653 0.875 1.092 0.003 0.443