3.5 2.

996 Concentration Absorbance

(µg/ml) At 570 nm
3
0 0
2.5 20 0.535
40 0.959
Absorbance

2 80 1.333
120 2.028
1.5
160 2.673
1 200 2.984
Unknown = 186 2.996
0.5

0
0 50 100 150 200 250
Concentration

0.6

0.5

0.4
Concentration Absorbance
Absorbance

0.3 (µg/ml) At 750 nm

0 0
0.2 10 0.042
0.06 20 0.093
0.1 40 0.197
60 0.308
0
0 20 40 60 80 100 120 80 0.348
Concentration 100 0.436
Unknown = 13 0.06