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    Bacte Pics Comp

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    Bacte Pics Comp

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    BACTE-PICS-COMP

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    of 14
    FIG. 14.1 Mierococel growing on sheep blood agar showing| yellow pigment. FIG. 14.2 Numerous gram-positive coed in clusters, with mam polymorphon clear cas from an aspirated abscess in staph) lococcal disease (Gram stain, original magnification x1000) FIG. 14.3 A, Microscopic morphology of Stephylococcus spp. (On Gram stain, gram-nogative-looking cells show how older cells decolorize (1000). B, Scanning electron micrograph showing the typical “clusters” of staphylococci («20,000). FIG. 14.4 Staphylocaccus aureus growing on sheep blood. showing B:hemolytic, creamy, buttery-looking coloni FIG, 14.5 Ceagulase:negative staphylococd growing on sheq blood agar, revealing nonhemolytic, white creamy colonies. FIG, 14.6 Tube coagulase test detects extracellular enzyme “free coagulase.” Top tube Is coagulase postive FG. 147 Novobiocn suscepti tes toclffereriate coagu- ase negatiesaphylocec! (CaNs)Kolated rom unne samples Phylococeun seprophytcu op) restart to nowsbiccn, indated bythe lack of sone of infbition around the dk FIG. 14.10 D-zone test~positive isolate showing flattening of the dindamydin (CC) zone adjacent to the erythromycin (E) disk and the characteristic D-lice pattern FG. 15.1 Gram stain of Spoeoeis. A, Solid madi. 8, quid medi FIG. 15.3 B-Hemolytic streptécoccal colonies on sheep blood agar. FIG. 15.4 a-Hemolytic streptococcal colonies on sheep bloo} agar FIG. 15.5 Streptococcus agalactiae colony gro blood agar. on she FIG. 15.2 the streptococci wal : OT of sputum from a patier with pneumonia caused by 5. pneu monige. ‘The lear, nonstained area around the organism Fepresents the capsule, (1000) FIG. 15.8 Streptococcus pneumoniae colonies on sheep blood agar. The colonies demanstrate a characteristic mucoid appear nce and 2 concave center. FIG. 15.9 Enterococcus species growing on sheep blood agar. FIG. 15.14 Colonies of Leuconostoc species growing on sheep blood agar (SBA). Leuconostoc spp. may produce a-hemolysis and can resemble viridans streptococci FIG. 15.17 Group A streptococci on sheep blood ager’ showin: susceptibility to bacitracin. Left, Susceptible. Right, Resistant. FIG. 15.18 Christie, Atkins and Mundh-Petersen test for presump- tivedentifiation of group 8 streptococ. Streptococas agalactine shows the classic arrow shape near the streptococcal streak FIG. 15.19 Modification of the Christie Atkins, and Munch Petersen test showing the enhanced hemolysis produced by Streptococcus agalactiae when a drop of extracted Blyrin & placed on the colon FIG. 15.21 Bile esculin test. Left Positive test shows blackening of the agar. Right. Negative test. FIG, 1520 Pyrrolidonyka-naphthylamide test for Streptococcus pyogenes and Enterococcus. Left, Negative. Right, Positive. FIG. 15.22 Streptococcus préumoniaé on blood agar showing susceptibiity to optochin. Left, Susceptible Streptococcus ant viidans streptococc, ms one wt : yo rap Gon 0 eis rin a mayo 1) 8 Me rls of Canc p00 Tauren Caey cane FIG. 16.3 Corynebacterium diphtheriae growing on sheap blood agar. (Courtesy Cathy Bissonette ) Z er. FIG. 16.6 Gram stain of Listeria monocytogenes in the bloo| (1000). (Courtesy Cathy Bissonette.) FIG. 16.7 Gram stain of Literla monocytogenes from 2 culture (1000): (Courtesy Steve Mahlen and Amanda Harrington ) G16 A, eas itis ap (4 te La morons vg 2 Ath em poo Smit ef coup Peep Cae (ae andl (Att ah Lerten ig” ho, coh teas the ope of ck, the urton wh the Sophos ares vec FIG. 16.9 Umbrella motility: Listeria. Motility test for Listeria ‘monocytogenes showing the typical * umbrella” pattern, which ‘occurs toward the surface of the medium when this organism is incubated at room temperature. Tube on left is positive; tube On right is negative control ee REIN Rear iy CF fuiptie Tas vn te edo along aren wich | seca 0 Kou Cty sone) FIG. 16.11 Comparison of colony morphology of Listeria (A) and Erysipelothric (B) growing on sheep blood agar (SBA) after 24 hours of incubation. (Courtesy Cathy Bissonette,) from a colony (1000). (Courtesy Steve Manien and Amanda Harrington.) FIG. 16.14 A, Gram staining of Nocardia demonstrating irregular staining (<1000). B, Acid-fat staining of Nocardia showing Dartialy acii-ast appearance (&1000). (Courtesy Steve Mahlen and Amanda Harrington.) FIG. 16.15 Appearance of sulfur granule collected from draining sinus tracts. These granules contain masses of filamentous ‘organisms with pus materials, The arrow poirts out eosinoph projections (clubs) characteristic of sulfur granules 'rom gram. Positive bacteria. (Hematoxylin and eosin stain, x1000). FIG. 16.16 Colony morphology of M ‘on chocolate agar. (Courtesy Steve Mahlen and Amanda Harrington) XN Coa FIG. 16.17 Gram staining of Bad (1000). (Courtesy Cathy Bieconette) FIG. 16.18 Spore stain of Bacillus. Vegetative cells are red; spores are green (1000). (Courtesy Cathy Bssonette) FIG. 16.19 Bacillis cereus on sheep blood agar. (Courtesy Cathy Bissonette) ‘ona James E. Martin Biological Environmental Chamber UJEMBE( plate inoculated in a characteristic "2" pattern, FIG. 17.3 Twenty-four-hour growth of Neisseria gonarthoea B FIG. 17.4 A, Direct Gram-stained smear ot male urethral dis charge showing intracellular and extracellular, gram-negative diplococcic (arrows), which s diagnostic of Newsseria gonorrhoeae (61000). 8, Diract smear with more than five polymorphonuclear neutrophils per field but no bacteria may suggest nongonocaccal urethritis (<1000) r y, FIG. 17.5 Neisseria gonorthoese colony morshology after hours of growth on modified Thayer-Martin (MTM) agar. FIG. 17.9 Direct Gram-stained smear of cerebrospinal fluid (CSF) illustrating intracellular and extracellular gram-negative dip- lococci (arrows) of NelSSeita meningitis (x1000), Fe NTA ‘ouponteig ety ont con, so tele ade pian eatin tn eee ed FIG. 17.10 Growth of MBS@H MEBIAGIGE after 48 hours on chocolate (CHO agar (left) and sheep blood agar (SBA) (igh. OF the dassic Neseria pathogens, only the meningococcus grows on SBA and CHOC ager FIG. 17.11 A, Direct Gramstained smear of an otitis media specimen illustrating intracellular, gram-negative diplocaces (61000). The organism was identified biochemically as M. catarrhalis from cultures. Blows GfMarseellalestSirialis after 48 hours, ilustrating the “wagon-whee!” appearance on chocolate agar FIG. 17.12 Colony morphology of MBiSeHalEMeFEa on sheep blood agar (48-hour culture). FIG. 17.13 Culture of NOSESASUSESMIG after 48 hours on sheep blood agar (left) and chocolate agar (right). This organism resembles Nevsseria meningitidis FIG. 17.14 Dry, wrinkled, breaderumb-like colony morphology of Neisseria Sicea on sheep blood agar (48-hour culture). FIG. 18.1 HB@m@pRIGSUAMUBAZE

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