Name & Roll No:

Thapar Institute of Engineering & Technology, Patiala

Department of Biotechnology
PBT306: Animal & Stem Cell Technology
M.Sc-Biotech Ord Semester)
MST
. 26th September 2018
Time: 02 Hours; MM: 25
Name of faculty: Siddharth Sharma, Ph.D

Note: Attempt all the following questions.

Section A

1. Explain the application of animal cell culture in the area of Genomics

(2)

2. Explain the disadvantages of using serum free media in animal cell culture (2)

3. How does cell shape and polarity play important role in process of differentiation
in vivo
cell culture (2)

4. Explain the process of cold trypsinization in the establishment of primary cell culture (2)

5. What are the steps involved in mechanical disaggregation during primary culture (2)

6. Explain the following (0.5 marks each)

(a) Pluronic-F68
(b) Passage
(c) Transferrin
(d) BSS

Section B

1. Describe the correlation between Viscosity, surface tension and foaming in cell culture
(3)
2. Explain how paracrine signaling plays an important role in cell differentiation
(3)
3. What are the reasons of not using serum in media when growing animal cells in large scale?
(3)
4. Explain the procedure of sub-culturing and the criteria of doing sub-culture (2+2)