1.

Purpose:

This STM is to explain step by step Procedure for estimation of Available

P2O5 in given sample.

2. Scope:

Method is applicable to GSSP.

3. Principle:

The plant can only consume available P2O5 as their food.

4. Apparatus:

4.1. UV Spectrophotometer.

4.2. Analytical Balance.

4.3. Water Bath Shaker.

4.4. Beaker 250ml.

4.5. Volumetric flask 500 ml.

4.6. Volumetric flask 100 ml.

4.7. Funnel.

4.8. Filter paper

4.9. Mortar.

4.10. Pipette 25ml, 5 ml.

5. Reagents:

5.1. Ammonium Mollybdovanadate.

5.2. Deminerlized H2O.

5.3. Ammonium citrate Solution.

6. Procedure:

6.1. Grind the GSSP/ROP in a mortar & carefully weigh 1 gm sample on

analytical balance.

Prepared by: ______________ Reviewed by: ________________

Chemist Lab In charge
 6.2. Transfer the weigh sample in 500 ml volumetric flask with Ammonium
citrate Solution (50 mL).
 Keep the flask in Water Bath Shaker for 60 Minutes at 650C with 100rpm
shaking speed? Make up the Volume after 30 minutes.
6.3. Filter a sizeable quantity (at least 50 %) in beaker.
6.4. Pipette out 5 ml from solution and put it in 100 ml volumetric flask, also
add 20 ml mollybdovanadate and make up the volume by demin water.
Stay for 10 minutes for color developing.
6.5. Take another 100 ml volumetric flask add 20 ml mollybdovanadate and
make up the volume by demin water. This is blank solution.
6.6. Power on the UV Spectrophotometer set at wave length 422 nm.
6.7. First wash the quevet with demin water and zero with blank solution.
6.8. After that take the reading of sample.
7. Calculation:

P2O5 content will be calculated using standard calibration curve.

8. References:

Prepared by: ______________ Reviewed by: ________________

Chemist Lab In charge