Operating Instructions Photometer 5010: Robert Riele GMBH & Co KG

Operating Instructions
Photometer 5010 V5+
ROBERT RIELE GmbH & Co KG
Software Version 7.3

Documentation Version 04.2019
5010e_73.docx / 23.04.19
SYMBOLS
The packaging material, the type plate on the instrument and the manual may contain the following symbols or
abbreviations:
Manufactured by:
This product fulfills the requirements of Directive 98/79/EC on

in vitro diagnostic medical devices.
In vitro diagnostic medical device
Caution (refer to accompanying documents)!

Please refer to safety-related notes in the manual accompanying this device.
Please consult instructions for use
Symbol for the marking of important information for appropriate handling of the device
Biohazard
Samples containing material of human origin must be treated as potentially infectious.
The relevant laboratory guidelines on safe use must be observed.
Symbol for the marking of electrical and electronics devices according to § 7 ElektroG
No special protection against penetrating moisture (IP = International Protection)

IP XO
Order number
REF
Serial number
SN
INSTRUMENT APPROVALS
The Photometer 5010 meets the requirements of Directive 98/79/EC on in vitro diagnostic medical devices (IVDD).
Furthermore, the Photometer 5010 is manufactured according to the special safety requirements for IVD medical devices
stated in EN 61010.
ROBERT RIELE GmbH & Co KG Page 2 GENERAL NOTES

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SAFETY INFORMATION
Operator qualification
Only appropriately trained operators are qualified to operate the device.
Environmental conditions
The Photometer 5010 is approved for indoor use only.
For further environmental conditions see chapter 10.1.
Patient ambience
The Photometer 5010 may not be used in the patient ambience.
Electrical Safety
This device was examined and left the factory in perfect technical condition. To preserve this and protect safe and
faultless operation, the operator must follow the orders and remarks of this operating manual.
Connect the device to grounded power outlets only. All peripheral devices that are connected to the Photometer
5010 must comply with safety standard EN 60950. Before connecting read the documentation of the peripheral
devices.
Opening covers or removing parts of the instrument, except where this can be achieved manually without the use
of any tool, may expose voltage-carrying components. Connectors can be live, too. Never try to maintain or repair
an open instrument, which is carrying voltage.
Only authorized specialist staff may carry out repairs at the device including replacement of the Lithium battery.
Through improper repairs, the warranty extinguishes, and the operator can be heavily jeopardized.
If suspected the device can no longer be operated safely, turn it off and take steps to ensure that no one will
subsequently attempt to use it.
Electromagnetic waves
Devices that emit electromagnetic waves may affect measured data, or cause the Photometer 5010 to malfunc-
tion. Do not operate the following devices in the same room where the Photometer 5010 is installed: mobile phone,
transceiver, cordless phone, and other electrical devices that generate electromagnetic waves.
Reagents
Regarding reagents follow the safety as well as the operating instructions of the manufacturers.
Pay attention to the currently valid German “Gefahrstoffverordnung” (GefStoffV)!
Biological safety
Liquid waste is potentially biologically hazardous. Always wear gloves if handling those materials. Do not touch
parts of the device other than those specified. Consult the laboratory protocol for handling biohazardous materials.
Pay attention to the currently valid German “Biostoffverordnung” (BioStoffV)!
Spillings and cleaning

If a sample is spilled on the device, wipe up immediately and apply disinfectant.
Waste
Handle liquid waste properly, according to legislation on water pollution, and on the treatment of drainage and
waste matter.

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MANUFACTURER’S WARRANTY
ROBERT RIELE GmbH & Co KG warrants Photometer 5010 against defects in material and workmanship.
For further information contact the local distributor.
WASTE MANAGEMENT NOTE
At the end of the life or utilization time the device and the accessories can be given back to the manufacturer with
costs for an environmental waste disposal. The previous professional decontamination has to be proved with a
certificate.
Address of the manufacturer:

Kurfuerstenstrasse 75-79
13467 BERLIN
GERMANY
Phone: +49 (0)30 404 40 87

Fax: +49 (0)30 404 05 29
E-Mail: info@riele.de
www.riele.de
QUALITY MANAGEMENT SYSTEM
ROBERT RIELE GmbH & Co KG maintains a quality management system according to ISO 13485, certified by
mdc medical device certification GmbH.

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CONTENTS
1 INTRODUCTION TO PHOTOMETER 5010 ................................................ 7

2 INSTALLATION ............................................................................................. 8
2.1 DELIVERY ........................................................................................................................................ 8
2.2 PREPARATION FOR INSTALLATION ............................................................................................ 8
2.3 INSTALLATION ................................................................................................................................. 8
2.4 LOADING PRINTER PAPER .......................................................................................................... 9
3 OPERATING ELEMENTS ........................................................................... 10

3.1 FRONT ............................................................................................................................................ 10
3.2 BACK .............................................................................................................................................. 10
3.3 TOUCHSCREEN............................................................................................................................. 11
3.4 WORKING AREA ........................................................................................................................... 11
3.5 CUVETTES AND CUVETTE ADAPTOR ...................................................................................... 12
3.5.1 Changing cuvette adaptor ....................................................................................................... 12
3.5.2 Working with original flow-through system .............................................................................. 12
3.5.3 Working with standard cuvettes .............................................................................................. 13
3.5.4 Working with discrete flow-through cuvette............................................................................. 13
4 PROGRAM SELECTION............................................................................. 14
4.1 Measurement with programmed methods ....................................................................................... 14
4.2 Measurement with basic methods ................................................................................................... 15
4.3 Method editor .................................................................................................................................. 15
4.4 Utility programs ............................................................................................................................... 16
4.5 Line feed [LF] .................................................................................................................................. 16
5 CALCULATION PROCEDURES ................................................................. 17

5.1 GENERAL NOTES ......................................................................................................................... 17
5.1.1 Fundamental to the handling ... .............................................................................................. 17
5.1.2 Fundamental to the tempering ... ............................................................................................ 17
5.1.3 Fundamental to the inputs ... .................................................................................................. 17
5.1.4 Fundamental to the methods with standard ... ........................................................................ 18
5.1.5 Fundamental to the methods with multi-standards ... ............................................................. 18
5.1.6 Fundamental to bichromatic measurements … ...................................................................... 18
5.1.7 Fundamental to the Kinetic….................................................................................................. 19
5.1.8 Fundamental to the methods with reagent blank… ................................................................. 22
5.1.9 Fundamental to ID-NO. and sample numerator… .................................................................. 22
5.1.10 Fundamental to storing test results… ..................................................................................... 22
5.2 ABBREVIATIONS ........................................................................................................................... 23
5.3 SURVEY OF THE METHODS ..................................................................................................... 24
5.4 DESCRIPTION OF METHOD PROCEDURES ............................................................................ 25
5.4.1 Calculation procedure 1 (C/F) ................................................................................................. 26
5.4.2 Calculation procedure 2 (C/F/Rb) ........................................................................................... 27
5.4.3 Calculation procedure 3 (C/F/Sb) ........................................................................................... 28
5.4.4 Calculation procedure 4 (C/F/SbRb) ....................................................................................... 29
5.4.5 Calculation procedure 5 (C/S)................................................................................................. 30
5.4.6 Calculation procedure 6 (C/S/Rb) ........................................................................................... 31
5.4.7 Calculation procedure 7 (C/S/Sb) ........................................................................................... 32
5.4.8 Calculation procedure 8 (C/S/SbRb) ....................................................................................... 33
5.4.9 Calculation procedure 9 (FTK/F/Rb) ....................................................................................... 34
5.4.10 Calculation procedure 10 (FTK/S/Rb) ..................................................................................... 35
5.4.11 Calculation procedure 11 (KIN/F/Rb) ...................................................................................... 36
5.4.12 Calculation procedure 12 (KIN/S/Rb) ...................................................................................... 37
5.4.13 Calculation procedure 13 (TRANSMISSION) ......................................................................... 38
5.4.14 Calculation procedure 14 (C/F Delta)...................................................................................... 39
5.4.15 Calculation Procedure 15 (C/F 3 WL) ..................................................................................... 41
5.4.16 Calculation Procedure 16 (DELTA R1R2) ............................................................................... 42
6 METHOD EDITOR ....................................................................................... 44
ROBERT RIELE GmbH & Co KG Page 5 CONTENTS

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7 UTILITY PROGRAMS ................................................................................. 47

7.1 SELECTION OF UTILITY PROGRAMS ....................................................................................... 47
7.2 DESCRIPTION OF UTILITY PROGRAMS ................................................................................... 48
7.2.1 Optic adjustment ..................................................................................................................... 48
7.2.2 Multi-standard functions .......................................................................................................... 49
7.2.3 Printer ON / OFF ..................................................................................................................... 50
7.2.4 Pump menu ............................................................................................................................ 51
7.2.5 Menu serial com ..................................................................................................................... 54
7.2.6 Quality control ......................................................................................................................... 55
7.2.7 Settings printout ...................................................................................................................... 59
7.2.8 Stored results.......................................................................................................................... 60
7.2.9 Temperature ON / OFF ........................................................................................................... 60
7.2.10 Temperature adjustment ......................................................................................................... 60
7.2.11 Laboratory name ..................................................................................................................... 61
7.2.12 User name .............................................................................................................................. 61
7.2.13 Error list .................................................................................................................................. 62
7.2.14 Key signal ON / OFF ............................................................................................................... 62
7.2.15 Touchscreen adjustment......................................................................................................... 62
7.2.16 Date / Time ............................................................................................................................. 63
7.2.17 Language ................................................................................................................................ 64
7.2.18 ADC counts (Optic) ................................................................................................................. 64
7.2.19 Bar Code................................................................................................................................. 64
7.2.20 Service tools ........................................................................................................................... 65
8 MAINTENANCE ........................................................................................... 66
8.1 CLEANING INSTRUCTION ........................................................................................................... 66
8.2 CALIBRATING MEASURING SYSTEM ........................................................................................ 66
8.3 ADJUSTMENT OF BUBBLE DETECTOR .................................................................................... 66
8.4 CALIBRATION OF PERISTALTIC PUMP .................................................................................... 66
8.5 REPLACEMENT OF PAPER ROLL ............................................................................................. 66
8.6 REPLACEMENT OF ASPIRATION TUBE ................................................................................... 67
8.7 REPLACEMENT OF LINE FUSES............................................................................................... 67
9 ERROR MESSAGE / CORRECTION .......................................................... 68

9.1 GENERAL NOTE ........................................................................................................................... 68
9.2 ACOUSTIC ERROR MESSAGES ................................................................................................. 68
9.3 PLAINTEXT ERROR MESSAGES ................................................................................................ 68
9.4 CODED ERROR MESSAGES ...................................................................................................... 68
10 TECHNICAL DATA ..................................................................................... 71

10.1 ENVIRONMENTAL CONDITIONS ................................................................................................. 71
10.2 MINIMAL OPERATION QUALITY ................................................................................................. 71
10.3 TYPE PLATE ................................................................................................................................. 71
10.4 SHORT SPECIFICATIONS ............................................................................................................ 72
10.5 TECHNICAL SPECIFICATIONS ..................................................................................................... 73
11 ACCESSORIES AND SPARE PARTS ....................................................... 75

12 METHOD LIST ............................................................................................ 76
12.1 BASIC METHODS.......................................................................................................................... 76
12.2 LIST OF USER SPECIFIC METHODS ....................................................................................... 77
ROBERT RIELE GmbH & Co KG Page 6 CONTENTS

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1 INTRODUCTION TO PHOTOMETER 5010
This device is a semi-automated, programmable photometer for In Vitro Diagnostic (IVD) by qualified laboratory
staff.
It is operated via touchscreen. Remote control is possible by a serial data interface (chapter 7.2.5 - Menu serial
com – REMOTE CONTROL).
For measuring methods several programmed methods with open parameters are available (chapter 5 -
CALCULATION PROCEDURES and chapter 12 - METHOD LIST).
Besides, up to 231 methods - built up on the basic methods - can be established and stored by the operator with
a method editor. A list of methods can be printed out (chapter 6 - METHOD EDITOR).
Up to 50 nonlinear calibration curves with maximum 20 sets of points can be stored (chapter 7.2.2 - Multi-standard
functions).
Photometer 5010 offers a flexible cuvette concept: A special flow-through cuvette and a peristaltic pump provide
for speedy operation with an optimal tempering of the measuring solution. Alternatively, the measuring solution
can be measured in one-way or glass cells in the provided standard cuvette adaptor.
By means of a combination of transistor and Peltier element the solution reaches fast and accurately one of the
three selectable temperatures, 25 °C, 30 °C or 37 °C.
The Photometer 5010 is standard equipped with six optical filters of the wavelengths 340, 405, 492, 546, 578 and
623 nm. If required, they can be exchanged against any wavelength within the range of 340-730 nm. Three
additional filters, e.g. 670 nm, can be installed.
The device is equipped with a thermal printer.
The measuring data can be stored and managed in the Photometer 5010 (chapter 7.2.8 - Stored results).
According to a GLP conformal documentation the names of lab and operator can be printed out as well as
transferred to EDP (chapter 7.2.5 - Menu serial com – EDP ON/OFF).
In Photometer 5010 up to 50 methods can be supervised with a quality control (chapter 7.2.6 - Quality control).
Numerous utility programs permit the individual configuration of the device. Function tests support the analysis of
sources of error.
Photometer 5010 is future-proof by FLASH MEMORY technology: The operating system can be updated with
program novelties and/or improvements comfortably, without having to open the equipment (please ask distributor
for further information).
ROBERT RIELE GmbH & Co KG Page 7 1 - INTRODUCTION TO PHOTOMETER 5010

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2 INSTALLATION
2.1 DELIVERY
Check the device and contents of the enclosed box as follows on visible transport damages and completeness:
1 Operator’s Manual
1 Aspiration tube
1 Dust cover
2 Fuses for line power
1 Mains cable
2 Thermal printer paper
1 Pump tube with joints
1 Standard cuvette adaptor
1 Top cover small for printer
1 Waste tube
Inform the sales office immediately about transport damages. Keep the original packaging for a possible
return.
2.2 PREPARATION FOR INSTALLATION
Place the device on a stable, level surface. Do not obstruct the input air at the bottom and the waste air at the
back plate to guarantee the ventilation of the device.
If the device was exposed to extraordinary fluctuation in temperature and/or humidity, it must acclimatize suffi-
ciently before operation.
Before connecting the waste tube to the pump tube remove pump tube at both ends from the metal clamps.
The waste tube of the flow-through system must be led through the tunnel to the backside of the device (chapter
3.2 - BACK) and then into any drain tank.
2.3 INSTALLATION
Photometer 5010 operates at any line voltage between 100 VAC and 240 VAC at 50/60 Hz. The device plug of the
mains cable must be put into the socket at the back of the device and the mains plug into a grounded mains
socket.
While connecting or disconnecting an external device (PC, printer) to Photometer 5010 both devices must
be switched off.
Switch on Photometer 5010 by the mains switch at the back.
Greeting screen:
( c ) R O B E R T R I E L E
After switching on copyright, website, type of
G m b H & C o K G device and version designation are
displayed and - in the case of activated
P H O T O M E T E R 5 0 1 0 printer - printed out.
V 7 . X a d d / m m / y y D
w w w . r i e l e . d e
ROBERT RIELE GmbH & Co KG Page 8 2 - INSTALLATION

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Indication for the direction of the light in

version 7 with LED.
[DELETE] Window will not be displayed next

time.
(Appears again when overwriting the

software or when initializing the system.)
[OK] Exit the window. Window appears

again next time you turn the device on.
M A I N M E N U After around 15 minutes the device is heated

up and ready for measurement.
First the tempering is switched off. If working
M E A S U R E W I T H P R O G R . M E T H O D S with tempered material is required later,
switch on the tempering already now either
M E A S U R E W I T H B A S I C M E T H O D S directly by the utility program (chapter 7.2.9
- Temperature ON / OFF) or indirectly by
M E T H O D S N E W / C H A N G E / C O P Y selection of a method with programmed
tempering (chapter 5.1 - GENERAL
NOTES).
U T I L I T I E S L F
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
If errors appeared during operation, first of all they have to be confirmed with [E] before remedy (chapter 9 -
ERROR MESSAGE / CORRECTION).
2.4 LOADING PRINTER PAPER
With initial operation or if the colored end of the paper roll appears, printer paper must be inserted:
• Open the printer cover.
• Put the green head-up lever in the up position.
• Remove the rest of paper.
• Put the green head-up lever in the down position.
• Put printer paper axis into the new printer paper reel.
• Insert the paper inside the printer. The roller will automatically feed the paper for about 4 cm.
• Press [LF] several times for line-feed until the paper has a length of about 5 cm. In case of no reaction
the printer may be deactivated.
• Insert printer paper reel into the axis guide.
• Push the printer paper through the slot in the printer cover and close the printer with the cover.
ROBERT RIELE GmbH & Co KG Page 9 2 - INSTALLATION

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3 OPERATING ELEMENTS
3.1 FRONT
printer cover
lid
working area
touchscreen
aspiration tube sipping lever [P]
3.2 BACK
ROBERT RIELE GmbH & Co KG Page 10 3 - OPERATING ELEMENTS

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3.3 TOUCHSCREEN
The touchscreen shows applications and
information. It is contact-sensitive and reacts
to the pressure exerted on it. In order to
execute a function, the desired range on the
screen must be touched
The surface of the touchscreen may

be never touched with ball-point pen, pencil
or another pointed article!
3.4 WORKING AREA
waste tubing
REF 1704834001 pump tube with joints
REF 5010-050
outlet tube cuvette

REF 5010-066
aspiration tubing
REF 5010-065
joint inlet tube cuvette

REF 1707175001

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3.5 CUVETTES AND CUVETTE ADAPTOR

3.5.1 Changing cuvette adaptor
If a flow system is installed, first the tubing system must be emptied: Go to the main menu and execute a washing
process by sipping lever [P] five times. Air is aspirated and the residual liquid is pumped off through the waste
tube.
Switch off the device.
If a flow system is installed, disconnect the bubble detector, remove the aspiration tube from the metal tube and
disconnect the outlet tube cuvette from the joints of the pump tube.
Loosen the milled screw and remove the adaptor.
Another adaptor can easily be inserted. Before check the cleanliness of the lense and the underside of the
adaptor. When inserting the adaptor electrical contacts in the device are closed. Turn milled screw only by hand!
After replacing of a cuvette adaptor by another one an optic adjustment should be done after reaching operating
temperature (see chapter 7.2.1 - Optic adjustment).
Direction of the light path:
Direction of the light
3.5.2 Working with original flow-through system

Lead the aspiration tube with attached bubble detector without sharp bend from the working area through the
metal tube. Fasten outlet tube cuvette to the metal nipple of the cuvette as well as to the joint of the pump tube.
Before connecting the tubes remove pump tube at both ends from the metal clamps. The pump tube must
not be tensed during longer time out (fig. below).
The lid of the cuvette compartment may stay open. The optic is not sensitive to stray light.

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Tension release:
The upper connection of
the pump tube can be
loosened easily from the
metal clamps.
Plug the bubble detector to the socket in the working area. It must sit as close as possible at the connection of
the flow-through cuvette. For working without bubble detector switch off this function (chapter 7.2.4.3 - Bubble
detector ON / OFF).
While working with the flow-through system the tubes must not be sharply bended. No residues should be
inside. Check from time to time that the tubing and the connections are leakproof. After each replacement of
tubing execute a Pump calibration (chapter 7.2.4.2).
Before as well as after all measuring it is absolutely necessary to wash the tube system repeatedly with
distilled water or another appropriate rinse solution by pressing [WASH] or sipping lever [P]. This is also necessary
after a method change (chapter 8.1 - CLEANING INSTRUCTION). For the procedure within a measuring series
see the application regulation.
In order to aspirate solution into the measuring system put the aspiration tube deeply enough into the respective
vessel.
Before setting to zero press [ZERO]. Trigger setting to zero by sipping lever [P].
Trigger a normal measuring by sipping lever [P]. Repeat a measuring of a solution which is already aspirated by
[RESULT].
To work in optimized volume mode use the function Volume optimized ON / OFF (chapter 7.2.4.3.2). This
function makes it possible, for example, to pump two consecutive times 500 µl from 1000 µl sample volume.
3.5.3 Working with standard cuvettes
The optical path is directed from left to right of the device. Insert single cuvette according to the drawing
OPTIC CONSTRUCTION in TECHNICAL DATA.
The lid of the cuvette compartment may stay open. The optic is not sensitive to stray light.
Trigger setting to zero by [ZERO].
Trigger a normal measuring by [RESULT].

3.5.4 Working with discrete flow-through cuvette
For use of a discrete flow-through cuvette insert the standard cuvette adaptor.
The optical path is directed from left to right of the device. Insert flow-through cuvette according to the drawing
OPTIC CONSTRUCTION in TECHNICAL DATA.
Pay attention to the correct connection of aspiration tube and outlet tube cuvette.
Switch on the pump by the function Pump ON / OFF (chapter 7.2.4.1).
Switch on or off bubble detector by the function Bubble detector ON / OFF (chapter 7.2.4.3).
After the installation execute a Pump calibration (chapter 7.2.4.2).

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4 PROGRAM SELECTION
After switch-on the touchscreen shows the main menu.

From this screen the basic methods (unalterably programmed in the system) or operator specific programmed
methods can be reached. In addition, the adjusting programs are started from this mask. With the method editor
own methods can be established and changed. The utility programs cover the configuration adjustments and
check routines. The line feed function of printer can directly be reached by [LF].
After completion of a method or execution of a utility program the program always returns to the main menu.
M A I N M E N U Main menu:
Down in the status line from left to right
following is shown:
M E A S U R E W I T H P R O G R . M E T H O D S
• Current temperature of the cuvette
M E A S U R E W I T H B A S I C M E T H O D S adaptor in °C.
In the case of switched off tempering the
M E T H O D S N E W / C H A N G E / C O P Y display changes between --.--C und
xx.xxC.
In the case of switched on tempering and
instable temperature the display
changes between --.--C and e.g. 37.3°C.
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9 In the case of stable temperature the
current temperature of e.g. 37.0°C is
shown. Small fluctuations of the value
are normal.
• Date in the format day/month/year
• Time
4.1 Measurement with programmed methods

A programmed method for a photometric
test can be called directly by input of the
method number.
M E T H O D S N O . 3 0
M E T H O D : G l u c o s e
The valid range for a method number lies
U N I T : m g / d l between 20 and 250.
1 2 3 4 5 6 7 8 Scroll all existing methods by [+] or [-]. If no
method is programmed, a plain text error
E S C 9 0 . + - E message (chapter 9.3 - PLAINTEXT
ERROR MESSAGES) is shown.
Call the selected method by [E].

Return to main menu by [ESC].
A programmed method can be estab-

lished via menu METHOD NEW /CHANGE /
COPY (chapter 4.3 - Method editor).
The transmission of a method collection is
possible by PC with special software.
Further information:
Application sheets of reagent manufacturers
ROBERT RIELE GmbH & Co KG Page 14 4 - PROGRAM SELECTION

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4.2 Measurement with basic methods

A photometric test can be executed by a method already permanently programmed, but open in all setting
parameters. 14 different methods with different calculation procedures are available. Each of these methods can
serve as prototype for a method programmed by the operator.
B A S I C M E T H O D S P A G E 1 / 4 Available are:
• Absorbance measurement
C O N C . W . F A C T O R P A G E
• Concentration measurement / end point
measurement
C O N C . W . F A C T O R R B
• Fixed time kinetic / two point kinetic
C O N C . W . F A C T O R S B • Kinetic
C O N C . W . F A C T O R R B S B E X I T • Transmission
Scrolling through all methods is possible by
[PAGE]. The current page is shown at the
right upper screen corner. By [END] the
program returns to the main menu.
A method is selected by pressing the corre-

sponding key.
The following abbreviations are used for the

distinction of the methods:
• CONC. = concentration
• KIN = kinetic
• FTK = fixed time kinetic
• F = factor
• STD = standard
• RB = reagent blank
• SB = sample blank
Chapter: 5 - CALCULATION
PROCEDURES
4.3 Method editor

M E T H O D N E W / C H A N G E / C O P Y Each photometric test can be permanently
stored with its setting parameters by the
method editor.
M E T H O D C O P Y L I S T
With the functions of the method editor are
M E T H O D E D I T possible the new installation, the change
and removing a method.
M E T H O D N E W
By [LIST] an overview of the programmed
methods can be printed and transmitted via
M E T H O D D E L E T E E X I T the serial interface.
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9 Further information:
Chapter: 6 - METHOD EDITOR

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4.4 Utility programs

Utility programs are necessary for the
U T I L I T I E S P A G E 1 / 5 adjustment and maintenance of
Photometers 5010.
O P T I C A D J U S T M E N T P A G E
M U L T I - S T A N D A R D Chapter: 7 - UTILITY PROGRAMS
P R I N T E R
P U M P M E N U E X I T
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
4.5 Line feed [LF]
M A I N M E N U Pressing [LF] in the main menu triggers a

line feed in the case of activated printer.
Several lines can be advanced by
M E A S U R E W I T H P R O G R . M E T H O D S continuous pressure on [LF].
M E A S U R E W I T H B A S I C M E T H O D S
M E T H O D S N E W / C H A N G E / C O P Y
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9

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5 CALCULATION PROCEDURES
5.1 GENERAL NOTES

The device offers operator guidance in the display by a combination of plaintext and short terms.
Messages and inputs regarding the method always have to be confirmed by [OK]. By [EXIT] all methods can be
broken off. For a restart see chapter 4 - PROGRAM SELECTION. Measuring is generally triggered by sipping
lever [P] or [RESULT], zero measuring by [ZERO] and sipping lever [P] (chapter 3.5.2 - Working with original flow-
through system and 3.5.3 - Working with standard cuvettes -).
5.1.1 Fundamental to the handling ...
• When measuring with standard cuvettes the lid of the cuvette compartment may stay open during
measurement.
• Deviations from normal operation, caused by the device or by the operator, are notified by
“ERROR”. They always have to be confirmed by [E] (chapter 9 - ERROR MESSAGE / CORRECTION).
Example 1: The reading exceeds the programmed upper limit

Example 2: Too little liquid when sucking in a measuring solution
5.1.2 Fundamental to the tempering ...
• Tempering switched on or off is parameter of a method.
• After switching on the tempering it lasts up to 10 minutes until a constant temperature of 25 °C, 30 °C or
37 °C is reached.
• The current temperature of the flow-through cuvette or of the cuvette adaptor is shown at the Iower edge
of the touchscreen. For meaning of the display see chapter 4 - PROGRAM SELECTION /MAIN MENU.
A temperature instable or out of tolerance during measuring is marked by an asterisk (*) at the utmost
right position in the corresponding print line.
To avoid deviations due to temperature influence a delay between triggering and actual measuring can
be programmed in each method.
• For a quick mode of operation all temperature-sensitive samples, reagents and washing solutions should
be externally tempered by Incubator T12/T16 (REF 500-002 / 500-001) or a water bath.
5.1.3 Fundamental to the inputs ...

• The input format of the factor and/or the standard with sign determines the output format of the result
concerning the number of decimal places.
Example: With factor “36.8” the calculated concentration will be shown with one decimal place.
• Each factor or standard can be minus signed, so that the result is calculated with correct sign.
Example: The test GOT is programmed with the factor “-1746“ because the measuring principle implies
a decreasing absorbance.
• For a homogeneous solution the input of a delay before a measuring is possible at all methods.
• All delay times can be cancelled by pressing the aspiration tube [P] for a long time.
ROBERT RIELE GmbH & Co KG Page 17 5 - CALCULATION PROCEDURES

5010e_73.docx / 23.04.19
5.1.4 Fundamental to the methods with standard ...

• Each measuring of a standard (calibrator) can be executed as single, double or triple determination.
Following is shown:
In the white reading window the

M E A S U R E S T G l u c o s e
averaged absorbance of the
0.615 S T [ A ]
standard is shown.
Below the white reading window

the absorbance 1, 2 and 3 of a
standard are shown.
0 . 6 1 6
0 . 6 1 2
0 . 6 1 7 By [OK] the average of all values is
taken over. Values with 0 are
ignored and excluded from the
O K C U R S . D E L . R E S U L T
calculation. The resulting factor is
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9 calculated from the average of the
standard.
By [CURS.] a value is selected. A

flashing white square marks the
current value.
By [DEL.] a value is deleted and

excluded from the calculation.
By [RESULT] a measuring is
triggered.
• The determined resulting factor of a standard measurement is stored together with the corresponding
method number. After renewed selection of this method the last resulting factor is offered as "OLD STD".
• The principle of the multiple measurement can also be expanded to all measurements. The correspond-
ing entry can be set invoking a basic method. The parameter is definable in preprogrammed methods
(chapter 6 - METHOD EDITOR).
5.1.5 Fundamental to the methods with multi-standards ...

• Linear calibration is used in the case of two calibrators. The absorbance forms a linear diagram with the
concentrations (chapter 7.2.2 - Multi-standard functions).
• Nonlinear calibration is used for samples with a nonlinear but reproducible connection between the
absorbance and the concentrations. At least three (maximum 20) calibrators are required for nonlinear
calibration (chapter 7.2.2 - Multi-standard functions).
5.1.6 Fundamental to bichromatic measurements …

• The calculation procedures based on endpoint measurement (CP 1 to CP 8, CP 13 and CP 14) can be
executed bichromatic. The zero measurement will be done with a wavelength defined as bichromatic.
The bichromatic wavelength might be not included in the standard set of filters. The bichromatic
wavelength can be set after calling a method (chapter 6 METHOD EDITOR Fig. 6.5).

5010e_73.docx / 23.04.19
5.1.7 Fundamental to the Kinetic…

In a kinetic method the sample absorbance is measured several times in pre-established time intervals.
The user can define a delay time and a quantity and duration of time intervals after the delay time (Deltas or Δt).
At the beginning and at the end of the delay time the absorbance values ABS.1 and ABS.2 are measured
respectively. The difference |ABS.1 – ABS.2| allows the differentiation between normal and abnormal activities.
This is followed by a sequence of measurements in regular time intervals (Deltas or Δt). An example of a resulting
curve is shown in Fig. 5.1.7.1:
Fig. 5.1.7.1: Resulting curve of kinetic test, decreasing absorbance
In each time interval (Delta or Δt) the difference between the relating absorbance values as well as the gradient
of the curve are calculated.
To obtain the alteration per minute AS,Minute the gradients must be averaged. This is done by a simple linear
regression calculation also giving an indicator for the linearity of the test. This indicator is called the coefficient of
correlation R. For practical reasons, the square of the coefficient of correlation R^2 or coefficient of determination
is taken in a Kinetic calculation. The value of R^2 can vary between 0 and 1. An R^2 value of 1 indicates perfect
linearity and a value of 0 indicates absolute non-linearity. Already values < 0.9 indicate a bad linearity and
therefore an incorrect test. In order to improve the linearity of the kinetic only the best three deltas are considered
in the calculation procedure of the regression calculation. Therefore, at least five deltas are required when
programming a new method. If this procedure does not lead to an improvement all deltas are reconsidered in the
calculation procedure.
In practice, linear tests show values of R^2 near to 1. In the example for Calculation procedure 11 (KIN/F/Rb)
values of R^2 ≥ 0.998 are permitted. Results with smaller R^2 values could be caused by temperature instability,
pollution, expired reagents, unfavorable delay time, etc.
For a better monitoring the number of deltas (deltas or Δt) should be bigger than specified for the manual
procedure. The classic three-minutes-test with three deltas of 60 s can be replaced by 15 deltas of 12s.
When programming a new method, which is based on CP 11 or CP 12, it is possible to set lower and upper limits
for the measurement result within the method editor (see chapter 6 - METHOD EDITOR, Fig. 6.5). This can be
achieved setting the parameters MIN. VALUE and MAX. VALUE. If the measured value exceeds the MAX.
VALUE, a message RANGE MAX. is shown, and if the measured value falls below, MIN. VALUE message
RANGE MIN. is shown. Also a lower limit for R^2 can be entered by setting MIN. R^2, if the obtained R^2 value
falls below the entered value a message NON-LINEAR is shown.
In order to get positive results at tests with decreasing absorbance (see Fig. 5.1.7.1), a negative factor has to be
entered. Only if MAX. VALUE is set and the sign of the measured value is not equal to the sign of the entered
MAX. VALUE a message RANGE +/- is shown.
The parameters MIN. VALUE, MAX. VALUE and MIN. R^2 are deactivated entering a zero value.

5010e_73.docx / 23.04.19
Presentation of the results on the display after a successful measurement:

View after the successful measurement
By [DiAG] the progress of the kinetic is shown.
View after confirming by [MODE].
View after pressing [MODE] [MODE]
By [PRN] the internal printer is switched off.

By [DETAIL] all immediate test results are
shown or printed.
By [ZERO] the zero measurement is repeated.
The measurement is repeated by [RETRY].
View after pressing [DIAG.]
The progress of the kinetic is shown after a few

seconds.
The axis of time is marked by [s], the axis of

extinction is marked by [ABS]
The currently used FACTOR and the calculated

DELTA REG are shown in the bottom lines.
If R^2 is activated, the term LINEAR or NON-

LINEAR is shown in the upper right corner. By
[PRINT] a graphical printout is generated. By
[LIST] all data points are shown sequentially.
Sequential View of data points after confirming

[DIAG] and [LIST]
By [NEXT] for each data point the numeration,

the time [s] and the extinction is shown.

5010e_73.docx / 23.04.19
Presentation of the results on the printout after a successful measurement:
PHOTOMETER 5010 #20001

V7.1a dd/mm/yy D
LAB.: RIELE BERLIN
USER 1: M.MUSTERMANN
DATE: 09/03/17
TIME: 08:44:12
METHOD 11: KIN/F/Rb
PROGRAM: 11
FACTOR: 4130.0
WAVELENGTH: 340nm
TEMPERATURE: 37C
DELAY: 180s
DELTAS: 6
TIME/DELTA: 30s
UNIT: U/l
- - - - - - - - - - - -
MEASURE ZERO
OLD Rb[A]: 0.000
NO. ABS. RESULT

1 0.107 150.8 ABS = ABS.2 - ABS.1
R^2: 0.9994 RESULT = DELTA REG x FACTOR
Printout after confirming [DIAGR.] and

[PRINT]
The chronic of the kinetic is printed. The

axis of time is marked by [s]; the axis of
extinction is marked by [ABS].
NO. TIME [s] ABS.
NO. TIME [s] ABS. In the next step a chart including all data
1 0 0.718
2 30 0.734 points is printed.
3 60 0.750
4 90 0.767
5 120 0.785
6 150 0.805
7 180 0.825
ABS.1: 0.642
ABS.2: 0.750 Printout after confirming [MODE], [MODE]
1: 0.0312 and [DETAIL].
2: 0.0320
3: x 0.0345 ABS/min
4: x 0.0364
5: x 0.0387 As of five programmed deltas in case of an
6: 0.0417
DELTA REG: 0.0365 improvement of the linearity the three best
deltas are considered and marked with a
“x”. If this procedure does not lead to an
improvement all deltas are reconsidered
in the calculation procedure and none of
the deltas are marked.

5010e_73.docx / 23.04.19
5.1.8 Fundamental to the methods with reagent blank…
After selecting a method with

reagent blank (RB), the reagent
blank can be measured, entered or
put on zero.
R E A G E N T B L A N K
Press [EDIT] to enter the RB
manually or [MEAS.] to measure it.
E D I T M E A S .
When using a programmed method

based on a calculation procedure
with RB, the used RB will be stored
together with the corresponding
O L D R B 0 . 1 1 0
method number.
After renewed selection of this

method the last stored reagent
N O Y E S blank is offered as “OLD RB”.
Press [YES] to use the last stored

RB or [NO] to enter or measure it.
5.1.9 Fundamental to ID-NO. and sample numerator…

• All test results are labeled with a numerator.
• Additionally, all results can be labeled with a 5-digit ID-NO. When the ID-NO. is not zero, it will be
displayed and printed together with the sample result.
• When a method is selected you can edit both numerator and ID-NO. of a sample with [MODE] [NUM.]
Press [N / I] to switch between
editing numerator and ID-NO.
N E W S A M P L E N O . 1 - 9 9
1 2 3 4 5 6 7 8
E S C 9 0 . + - N / I E
5.1.10 Fundamental to storing test results…

• All test results are stored automatically. Up to 1000 results can be stored in memory.
• See Table 7.2.5.1 for the format of the stored data.
• Stored results can be output through serial interface (chapter 7.2.8 - Stored results).
• When memory is full oldest test results will be overwritten or you can send all results through the serial
interface and then delete them.

5010e_73.docx / 23.04.19
5.2 ABBREVIATIONS
A, ABS .................. Absorbance

ARB ........................ Absorbance of reagent blank
ARB,0 ...................... At Fixed Time: absorbance of reagent blank after incubation time T0
ARB,1 ...................... At Fixed Time: absorbance of reagent blank after reaction time T1
ARBB ....................... Absorbance of blank of reagent blank
AS .......................... Absorbance of sample
AS,0 ........................ At Fixed Time: absorbance of sample after incubation time T0
AS,1 ........................ At Fixed Time: absorbance of sample after reaction time T1
ASB ........................ Absorbance of sample blank
AST......................... Absorbance of standard
AST,0....................... At Fixed Time: absorbance of standard after incubation time T0
AST,1....................... At Fixed Time: absorbance of standard after reaction time T1
ASTB ....................... Absorbance of standard blank
C ........................... Concentration
CST ........................ Concentration of standard
CV ......................... Quality control: Coefficient of variation
dA/min ................... At Kinetic: ΔA / min
ARB,Minit ................ At Kinetic: change of reagent blank per minute (measured in A / min)
AS,Minit .................. At Kinetic: change of sample per minute (measured in A / min)
F ............................ Factor
FTK ....................... Fixed Time Kinetic
KIN ........................ Kinetic
n ............................ Quality control: number of values
nm ......................... Nanometer (dimension of wavelength)
m ........................... Quality control: mean of values
R ........................... Result, Sample
Rb ......................... Reagent blank
Rbb ....................... Blank of reagent blank
R^2 ........................ At Kinetic: square of correlation coefficient
or coefficient of determination shows the linearity of a test
S, ST ..................... Standard
STb ....................... Standard blank
Sb .......................... Sample blank
s ............................ Quality control: standard deviation
TRANSM., T .......... Transmission in %
T0 .......................... At Fixed Time: incubation time in seconds
T1 .......................... At Fixed Time: reaction time in seconds
T1 .......................... At Kinetic: time per delta in seconds

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5.3 SURVEY OF THE METHODS

The calculation procedures, on which all methods are traceable from the list of methods, are mentioned in the
following table. Criterion is the characteristic of the calculation procedure (see below). For detailed description of
the respectively accompanying procedure of method see chapter 5.4 - DESCRIPTION OF METHOD
PROCEDURES.
CP-No. Characteristic Method Calculation formula

CP 1 C/F Endpoint with Factor C = F  AS
CP 2 C/F/Rb Endpoint with Factor C = F  (AS - ARB)
CP 3 C/F/Sb Endpoint with Factor C = F  |AS - ASB|
CP 4 C/F/SbRb Endpoint with Factor C = F  (|AS - ASB| - |ARB - ARBB|)
CP 5 C/S Endpoint with Standard C = F  AS
CP 6 C/S/Rb Endpoint with Standard C = F  (AS - ARB)
CP 7 C/S/Sb Endpoint with Standard C = F  |AS - ASB|
CP 8 C/S/SbRb Endpoint with Standard C = F  (|AS - ASB| - |ARB - ARBB|)
CP 9 FTK/F/Rb Fixed Time Kinetic with Factor C = F  ( |AS,0 - AS,1| - |ARB,0 - ARB,1| )
CP 10 FTK/S/Rb Fixed Time Kinetic with Standard C = F  ( |AS,0 - AS,1| - |ARB,0 - ARB,1| )
CP 11 KIN/F/Rb Kinetic with Factor C = F  ( AS,Minit - ARB,Minit )
CP 12 KIN/S/Rb Kinetic with Standard C = F  ( AS,Minit - ARB,Minit )
CP 13 TRANSM. Transmission in %
CP 14 C/F DELTA Endpoint with Factor C = F  ( AS2-Sb2 - AS1-Sb1 )
CP 15 C/F 3 WL Measurement with 3 Wavelengths C = 168 * A415nm – 84 * A380nm – 84 *
A450nm
CP 16 DELTA R1R2 Diff. measurement of two reagents C = AS
Explanations:
CP-No. ........................Number of the calculation procedure (chapter 6 - METHOD EDITOR)
Characteristic ..............Name of the calculation procedure (chapter 12.1 - BASIC METHOD)
Calculation formula .....Calculation basis of basic method

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5.4 DESCRIPTION OF METHOD PROCEDURES

In the descriptions of the calculation procedures a typical print-out by the internal printer is shown on the left side.
All examples were built with active pump. In case of measurements without pump the volumes for sipping the
media are not printed.
All print-outs begin with the device information, laboratory data and method parameters followed by all measuring
data necessary for a manual examination of the readings.
The measuring window
The arrangement of the measuring window is alike in all calculation procedures. Depending on the method,
various numbers of readings or diagrams are shown.
status of measuring system or name of active method

next action
M E A S U R E S A M P L E G l u c o s e
reading window
numerator
16.5 Additional method
specific information
3 m m o l / l
ID number I D - N O . 1 3 4 6 7
dimension
action keys
E X I T M O D E W A S H Z E R O R E S U L T
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
info line
Functions of the action keys in the measuring window:

[EXIT] Leads to the query whether the measuring program is to be terminated
[MODE] Occupies the action keys with following mode functions:

[NUM.] [MODE] [LF] [QC] [RETURN]
[PRN] [DETAIL] [M-STD] [RETURN]
[PRN] [DETAIL] [ZERO] [RETRY] [RETURN]
[PRN] [DETAIL] [ZERO] [E1/E2] [RETURN]
[WASH] With activated pump the wash volume defined to the method is pumped
[ZERO] Starts the zero measuring

With activated pump the sipping lever [P] must also be pressed
[RESULT] Starts the measuring

With activated pump a solution already sipped is measured once again
To aspirate and measure a new sample press sipping lever [P]
Mode functions:
[NUM.] Edit sample numerator or ID-NO. (chapter 5.1.9)
[LF] Line Feed
[QC] Quality Control functions
[PRN] Switch printer ON/OFF / printout of current time by [TIME]
[DETAIL] Display/printout of detailed results
[M-STD] Multi standard functions
[E1/E2] Change to measuring E2 (chapter 5.4.14)
[RETRY] Repeat a measurement
[RETURN] Return to normal functions

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5.4.1 Calculation procedure 1 (C/F)

Method at which a measured sample value AS is multiplied with a predefined factor F.
Calculation procedure .......................................................CP 1

Characteristic .................................................................... C / F
Method ....................................................End Point with Factor
Calculation formula .................................................. C = F  AS
Factor ............................................................... given / entering
Start method selection in the main menu.

PHOTOMETER 5010 #20001 See chapter:
V7.1a dd/mm/yy D 4.1 - Measurement with programmed methods
LAB.: RIELE BERLIN 4.2 - Measurement with basic methods
DATE: 09/03/17 In the case of activated printer the print-out of the method data
TIME: 08:44:12 follows.
METHOD 20: HEMOGLOBIN
PROGRAM: 1 The measuring window is shown.
FACTOR: 29.4
WAVELENGTH: 405nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 5s
MAX.UNITS: 25 Method procedure:
UNIT: g/l
- - - - - - - - - - - - →Insert / measure zero solution
MEASURE ZERO
NO. ABS. RESULT

1 0.675 19.8 →Insert / measure sample
2 0.843 24.8
→Insert / measure sample

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5.4.2 Calculation procedure 2 (C/F/Rb)

Method at which the difference of sample value AS and reagent blank ARB is multiplied with a given factor F.
The reagent blank ARB is entered or measured once.

Characteristic ............................................................ C / F / Rb
Calculation formula ....................................... C = F  (AS - ARB)
Reagent blank ........................................ entering or measuring

METHOD 21: HDL-C
FACTOR: 325
WAVELENGTH: 546nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 5s Method procedure:
UNIT: mg/dl
MEASURE ZERO
→Insert / measure reagent blank
Rb[A]: 0.058
NO. ABS. RESULT

1 1.064 327 →Insert / measure sample
2 1.188 367

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5.4.3 Calculation procedure 3 (C/F/Sb)

Method at which the difference of sample value AS and sample blank ASB regarding the amount is multiplied with
a given factor F. The sample blank ASB is measured before every test.

Characteristic ............................................................ C / F / Sb
Calculation formula ....................................... C = F  |AS - ASB|

METHOD 23: BILIRUBIN
FACTOR: 12.80
WAVELENGTH: 546nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 5s
MAX.UNITS: 8.0 Method procedure:
UNIT: mg/dl
MEASURE ZERO
NO. ABS. RESULT →Insert / measure sample blank

Sb[A]: 0.671
2 1.215 4.25 →Insert / measure sample blank
Sb[A]: 0.884 →Insert / measure sample
3 1.033 4.23
Sb[A]: 0.702 →Insert / measure sample blank

5010e_73.docx / 23.04.19
5.4.4 Calculation procedure 4 (C/F/SbRb)

Method at which the difference of reagent blank A RB and blank of reagent blank ARBB regarding the amount is
subtracted of the difference of sample value AS and sample blank ASB regarding the amount, and this difference
is multiplied with a given factor F.
The sample blank ASB is measured before every test. The reagent blank ARB is entered or measured once.

Characteristic ........................................................ C / F / SbRb
Calculation formula .............. C = F  ( |AS - ASB| - |ARB - ARBB| )

METHOD 24: Fe
FACTOR: 1330
WAVELENGTH: 578nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 5s
MIN.UNITS: 37
UNIT: ug/dl
MEASURE ZERO
→Insert / measure blank of reagent blank
Rb[A]: 0.085 →Insert / measure reagent blank
Rbb[A]: 0.198 (Resulting blank)
DELTA Rb: 0.113

Sb[A]: 0.486
2 0.646 49 →Insert / measure sample blank

5010e_73.docx / 23.04.19
5.4.5 Calculation procedure 5 (C/S)

Method at which a measured absorbance value AS is multiplied with a factor F which is determined by measuring
of a standard solution with known concentration CST.

Characteristic ....................................................................C / S
Method ............................................... End Point with Standard
Calculation formula .................................................. C = F  AS
Resulting factor .................................................... F = CST / AST

METHOD 25: GLUCOSE
STANDARD: 5.55
WAVELENGTH: 546nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 3s
MAX.UNITS: 22.2 Method procedure:
UNIT: mmol/l
MEASURE ZERO
→Insert / measure standard 1
ST[A] 1: 1.110 →Insert / measure standard 2 (optional)
ST[A] 3: 1.059
(Averaged standard)
ST[A]: 1.088 (Resulting factor)
FACTOR: 5.10
NO. ABS. RESULT

2 1.357 6.92

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5.4.6 Calculation procedure 6 (C/S/Rb)

Method at which the difference of sample value AS and reagent blank ARB is multiplied with a factor F which is
determined by measuring of a standard solution with known concentration C ST and under consideration of reagent
blank ARB.
The reagent blank ARB is entered or measured once.

Characteristic ............................................................ C / S / Rb
Calculation formula ....................................... C = F  (AS - ARB)
Resulting factor ........................................... F = CST / (AST-ARB)

METHOD 26: SODIUM
STANDARD: 150.0
WAVELENGTH: 405nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 3s
UNIT: mmol/l
MEASURE ZERO
→Insert / measure reagent blank
Rb[A]: 0.108
ST[A] 3: 1.118
(Averaged standard)
ST[A]: 1.121 (Resulting factor)
FACTOR: 148.2
NO. ABS. RESULT

2 1.118 149.6

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5.4.7 Calculation procedure 7 (C/S/Sb)

Method at which the difference of sample value AS and sample blank ASB regarding the amount is multiplied with
a factor F which is determined by measuring of a standard solution with known concentration C ST and under
consideration of standard blank ASTB.
The sample blank ASB is measured before every test.

Characteristic ............................................................ C / S / Sb
Calculation formula ....................................... C = F  |AS - ASB|
Resulting factor ........................................ F = CST / |AST - ASTB|

METHOD 27: UREA COL
STANDARD: 50.0
WAVELENGTH: 546nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 3s
UNIT : mg/dl
MEASURE ZERO →Insert / measure standard blank

ST[A] 3: 0.620 (Averaged standard)
(standard blank)
ST[A]: 0.621 (Averaged standard minus standard blank)
STb[A]: 0.106 (Resulting factor)
DELTA ST: 0.515
FACTOR: 97.1

Sb[A]: 0.257
3 2.223 197.2

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5.4.8 Calculation procedure 8 (C/S/SbRb)

Method at which the difference of reagent blank A RB and blank of reagent blank ARBB regarding the amount is
subtracted of the difference of sample value AS and sample blank ASB regarding the amount and this difference
is multiplied with a factor F which is determined by measuring of a standard solution with known concentration
CST and under consideration of standard blank ASTB and the difference of reagent blank ARB and blank of reagent
blank ARBB
The sample blank ASB is measured before every test. The reagent blank ARB is entered or measured once.

Characteristic ........................................................ C / S / SbRb
Calculation formula .............. C = F  ( |AS - ASB| - |ARB - ARBB| )
Resulting factor ................... F = CST / ( |AST-ASTB| - |ARB-ARBB| )

METHOD 28: Ca
STANDARD: 8.02
WAVELENGTH: 546nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 3s
MAX.UNITS: 12
UNIT: mg/dl
- - - - - - - - - - - - Method procedure:
MEASURE ZERO →Insert / measure zero solution
Rb[A]: 0.150 →Insert / measure blank of reagent blank

Rbb[A]: 0.046 →Insert / measure reagent blank
DELTA Rb: 0.104 (Resulting blank)
ST[A] 1: 1.485
ST[A] 2: 1.521 →Insert / measure standard blank
ST[A] 3: 1.495 →Insert / measure standard 1
→Insert / measure standard 2 (optional)
ST[A]: 1.501 →Insert / measure standard 3 (optional)
STb[A]: 0.479
DELTA ST: 1.022 (Averaged standard)
FACTOR: 8.74 (standard blank)
(Averaged standard minus standard blank)
NO. ABS. RESULT (Resulting factor)
1 1.495 7.89
Sb[A]: 0.489
3 1.394 8.39
→Insert / measure sample blank


5010e_73.docx / 23.04.19
5.4.9 Calculation procedure 9 (FTK/F/Rb)

Method at which a reagent blank is measured after an incubation time ( ARB,0) and after a reaction time (
ARB,1) and also a sample after an incubation time ( AS,0) and after a reaction time ( AS,1).
The difference from the change of the test and the change of the reagent blank is multiplied by a predefined factor
F. The reagent blank ARB is entered or measured once.
During the procedure the dialog asks for the use of a reagent blank. The default value is OFF. To continue with-
out reagent blank press [NO].
After each measurement the next sample can be measured with [NEXT].
With [RESULT] it is possible to measure the same sample again.

Characteristic ........................................................ FTK / F / Rb
Method ................................................. Fixed Time with Factor
Calculation formula ......... C = F  ( |AS,0 - AS,1| - |ARB,0 - ARB,1| )

METHOD 29: CK-MB
FACTOR: 2751.3
WAVELENGTH: 340nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
INCUBATION: 120s
REACTION: 180s
MAX.UNITS: 1500
UNIT: U/l Method procedure:
MEASURE ZERO
Without reagent blank (insert / measure optionally)
Rb[A]: 0.145
DELTA [A]: 0.121
NO. ABS. RESULT

1 0.346 -0.14 →Insert / measure sample
DELTA [A]: 0.053
2 1.029 1128.1
DELTA [A]: 0.410 →Insert / measure sample
3 0.829 1381.2
DELTA [A]: 0.502

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5.4.10 Calculation procedure 10 (FTK/S/Rb)

Method at which a reagent blank is measured after an incubation time ( ARB,0) and after a reaction time (
ARB,1) and also a sample after an incubation time ( AS,0) and after a reaction time ( AS,1).
The difference from the change of the sample and the change of the reagent blank becomes multiplied with a
factor F which is determined by means of the change of standard solution |A ST,0-AST,1| and the change of reagent
blank |ARB,0-ARB,1| during the reaction time and given concentration of standard. The reagent blank A RB is entered
or measured once.
out reagent blank press [ENTER].
After each measurement the next sample can be measured with [NEXT].
With [RESULT] it is possible to measure the same sample again.
Calculation procedure ..................................................... CP 10

Characteristic ........................................................ FTK / S / Rb
Method ............................................. Fixed Time with Standard
Calculation formula ......... C = F  ( |AS,0 - AS,1| - |ARB,0 - ARB,1| )
Resulting factor .............. F = CST / ( |AST,0-AST,1| - |ARB,0-ARB,1| )

METHOD 30: CREATININ
STANDARD: 2.00
WAVELENGTH: 492nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
INCUBATION: 45s
REACTION: 60s
UNIT: mg/dl
MEASURE ZERO
Rb[A]: 0.000
DELTA Rb: 0.000 →Insert / measure standard 1
ST/KIN 1: 0.194 →Insert / measure standard 3 (optional)
ST/KIN 2: 0.203 (Averaged standard)
ST/KIN 3: 0.214 (Resulting factor)
ST/KIN: 0.204
FACTOR: 9.80
NO. ABS. RESULT

DELTA [A]: 1.005
2 0.336 10.81
DELTA [A]: 1.103 →Insert / measure sample
3 0.329 12.84
DELTA [A]: 1.310

5010e_73.docx / 23.04.19
5.4.11 Calculation procedure 11 (KIN/F/Rb)

Method at which a sample S is measured several times (depending on the number of deltas) in an equidistant
time grid. From the resulting absorbance values an alteration per minute AS,Minute is determined by a linear
regression calculation. The reagent blank ARB,Minute is measured in the same way as the sample (or entered
directly in U/l) and subtracted from the sample value. This difference is multiplied by a given factor F. When
running a kinetic test with decreasing absorbance the factor F should be minus signed (e.g. F = -1746) in order
to get a positive result. The factor F should be positive for tests with increasing absorbance.

Characteristic ......................................................... KIN / F / Rb
Method ........................................................ Kinetic with Factor
Calculation formula ..................... C = F  ( AS,Minit - ARB,Minit )
Number of deltas ........................................... entering (3 to 19)
Time per delta ........................................ entering (4 s to 255 s)

METHOD 31: GOT
FACTOR: - 1746
WAVELENGTH: 340nm This example shows a negative factor producing a positive result
TEMPERATURE: 37C at decreasing absorbance.
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul The absorbance value will be constantly refreshed on the display
DELAY: 60s during the delay time.
DELTAS: 10 At the beginning and at the end of the delay time, the absorbance
TIME/DELTA: 6s values ABS.1 and ABS.2 are measured respectively.
MAX.UNITS: 280
MIN.R^2: 0.998
UNIT: U/l Method procedure:
- - - - - - - - - - - -
Rb[A]: 0.000 Without reagent blank (insert / measure optionally)

DELTA Rb: 0.000
R^2: 0.9762 →Insert / measure sample
Numerator / |ABS.1 – ABS.2| / Result
NO. ABS. RESULT R^2: coefficient of determination, used for linearity control of the
1 0.123 189 test (see chapter 5.1.7 Fundamental to the Kinetic…).
R^2: 0.9996
R^2: 0.9993
3 0.209 96
R^2: 1.0000 →Insert / measure sample
A detail print-out of ABS.1, ABS.2 and deltas can be made after

each measurement with [MODE] [MODE] [DETAIL] (see chapter
5.1.7 Fundamental to the Kinetic…).

5010e_73.docx / 23.04.19
5.4.12 Calculation procedure 12 (KIN/S/Rb)

Method at which a sample S is measured several times (depending on the number of deltas) in an equidistant
time grid. From the resulting absorbance values an alteration per minute AS,Minute is determined by a linear
regression calculation. The reagent blank ARB,Minute is measured in the same way as the sample (or entered
directly in U/l) and subtracted from the sample value. This difference is multiplied by a factor F which is determined
by measuring of a standard solution AST,Minute with known concentration CST and under consideration of the
reagent blank ARB,Minute.

Characteristic ......................................................... KIN / S / Rb
Method .................................................... Kinetic with Standard
Calculation formula ..................... C = F  ( AS,Minit - ARB,Minit )
Resulting factor ....................... F = CST / ( AST,Minit - ARB,Minit )
Number of deltas ........................................... entering (3 to 19)
Time per delta ........................................ entering (4 s to 255 s)

METHOD 32: UREA
STANDARD: 100.0
WAVELENGTH: 340nm
TEMPERATURE: 37C The absorbance value will be constantly refreshed on the display
MEAS. VOLUME: 900ul during the delay time.
WASH VOLUME: 1000ul At the beginning and at the end of the delay time, the absorbance
DELAY: 5s values ABS.1 and ABS.2 are measured respectively.
DELTAS: 5
TIME/DELTA: 8s
MIN.R^2: 0.998 Method procedure:
UNIT: mg/dl
MEASURE ZERO
Rb[A]: 0.000
Rb/KIN: 0.000 →Insert / measure standard 1
R^2: 0.1973
ST/KIN 1: 0.327
R^2: 0.9996 →Insert / measure standard 3 (optional)
ST/KIN 2: 0.330
R^2: 0.9989 (Averaged standard)
ST/KIN 3: 0.324 (Resulting factor)
R^2: 0.9994
ST/KIN: 0.327
FACTOR: 244.3 →Insert / measure sample
Numerator / |ABS.1 – ABS.2| / Result
NO. ABS. RESULT R^2: coefficient of determination, used for linearity control of the
1 0.232 41.5 test (see chapter 5.1.7 Fundamental to the Kinetic…).
R^2: 0.9984
R^2: 0.9997
A detail print-out of ABS.1, ABS.2 and deltas can be made after
each measurement with [MODE] [MODE] [DETAIL] (see chapter
5.1.7 Fundamental to the Kinetic…).

5010e_73.docx / 23.04.19
5.4.13 Calculation procedure 13 (TRANSMISSION)

Characteristic ................................................................. T in %

METHOD 13: TRANSM.
FACTOR: 1.0
WAVELENGTH: 546nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 2s
UNIT: % Method procedure:
→Insert / measure zero solution
MEASURE 100%
NO. ABS. RESULT →Insert / measure sample

3 2.020 1.0

5010e_73.docx / 23.04.19
5.4.14 Calculation procedure 14 (C/F Delta)

Method at which a difference of sample E2 – E1 is measured several times depending on the quantity of samples.
In the first course the samples E1 (maximum 25) will be measured, optionally with or without sample blank. After
a user defined measure time the samples E2 will be measured in a second course. Attention should be paid to
the order within the series to avoid errors. The procedure corresponds to a fixed time kinetic and can only be
processed using the standard cuvette adaptor.
Quality control samples cannot be saved.
This calculation procedure has special parameters that allow a time controlled measuring process. These
parameters are: time/delta T1, measure time T2, delay T3, reagent time #2 and reagent time #3. By setting a
time/delta (value between 10s and 255s) the other parameters for the time controlled measuring will be used. In
the time controlled mode the quantity of samples is determined by the measure time and the time/delta, e.g. with
a measure time of 60s and a time/delta of 10s it is possible to measure 6 samples (without sample blank). The
measure time should be chosen greater than or equal to the time/delta.
At the beginning of the method the use of a sample blank is queried.

After the blank is measured the time controlled measuring process will be started with [RESULT]. With a
combination of acoustic signals and text messages on the display the Photometer takes control of the timing for
the whole measuring. The measurement of the samples E1 can be finished at any time with [E1/E2]. In the second
course will be so many samples measured as in the first course.
Before starting a new E1/E2 course a new zero measurement has to be done.
The reagent time #3 is usable only if the reagent time #2 is set. In that case the reagent mode will be entered, i.e.
the photometer also takes control of the timing for dispensing the reagent before measuring the samples. The
quantity of samples will be determined by the reagent time #2 and the time/delta. The reagent time #2 should be
chosen less than or equal to the reagent time #3 and greater than or equal to the time/delta.
Figure 5.4.14.1 shows the time sequence of a time controlled measuring process with N samples, a delay time
T3 and without reagent time.
E11 E12 E1N E21 E22 E2N

meas. meas. meas. meas. meas. meas.
A E1 E2
... T3 T1 T1
... T1 T1
...
T2
Blank- Press Insert Insert Insert Insert Insert Insert

measure Result E11 E12 E13 E21 E22 E23
t: Time
T1: Time/Delta : automatic process
T2: Measure time
T3: Delay, optional : manual process
A: Start of time controlled measuring
E1: Start of first course (E1)
E2: Start of second course (E2)
Fig. 5.4.14.1: time controlled measuring

5010e_73.docx / 23.04.19

Characteristic ........................................................ C / F / Delta
Method ................................................... Difference with Factor
Calculation formula ..................... C = F  ( AS2-Sb2 - AS1-Sb1 )
Sample blank ....................................................... with / without
Time / Delta T1.................................. entering (0, 10s to 255s)
Measure time T2 .....................................entering (0 to 1800s)
Delay T3 ..................................................entering (0 to 1800s)
Reagent time #2 .......................................entering (0 to 1800s)
Reagent time #3 .......................................entering (0 to 1800s)

DATE: 09/03/17 In the case of activated printer the print out of the method data
METHOD 14: C/F DELTA
PROGRAM: 14
FACTOR: 1.000
WAVELENGTH: 405nm
TEMPERATURE: 37C
TIME/DELTA: 12s
MEASURE TIME: 100s The measuring window is shown.
DELAY: 10s
REAGENT 2: 40s
REAGENT 3: 60s Method procedure with sample blank:
UNIT: U/l
MEASURE ZERO
NO. Sb[A] S[A]E1 →Measure all samples E1 (maximum 25)

1 0.083 0.411
2 0.110 0.382 →Change to measuring E2 by [E1/E2]
3 0.146 0.492
→Measure all samples E2 (maximum 25)
NO. Sb[A] S[A]E2
1 0.091 1.090
2 0.140 0.991
3 0.200 1.165
Results based on the differences of the measured samples
NO. RESULT
1 0.671 →Show the results by [MODE] [MODE] [DETAIL]
2 0.578
3 0.619 Method procedure without sample blank:

1 0.000 1.012
2 0.000 1.138 →Change to measuring E2 by [E1/E2]
3 0.000 1.076

1 0.000 1.458
2 0.000 1.530
3 0.000 1.384
NO. RESULT Results based on the differences of the measured samples

1 0.446
2 0.392 →Show the results by [MODE] [MODE] [DETAIL]
3 0.307

5010e_73.docx / 23.04.19
5.4.15 Calculation Procedure 15 (C/F 3 WL)

Method at which a sample is measured with three different wavelengths: 380 nm, 415 nm and 450 nm. This
method is appropriated for free hemoglobin measurements.
The wavelength 380 nm is only avalilable in combination with a halogen lamp optic. Please contact the
manufacturer for further details.
The mentioned wavelengths are not included in the standard set of filters.
The factor has to be adjusted if using thinner (see chapter 5.1.3).
Calculation procedure ..................................................................................... CP 15

Characteristic ............................................................................................ C / F 3 WL
Method .................................................................. Measurement with 3 wavelengths
Calculation formula .............. C [mg/dl]= F * (168 * A415nm – 84 * A380nm – 84 * A450nm)
Factor ................................................................................................. given / entering
Conversion factor ............................................................... µmol/L ≡ 0,6206 * mg / dl

METHOD 15: C/F 3 WL
FACTOR: 1.00
WAVELENGTH:
380/415/450nm
TEMPERATURE: 37C
DELAY: 2s
UNIT: mg/dl
- - - - - - - - - - - -
NO. µmol/L mg/dl

1 4.859 7.83 →The result will be displayed in two units of measurement
2 5.865 9.45

5010e_73.docx / 23.04.19
5.4.16 Calculation Procedure 16 (DELTA R1R2)

Method for two endpoint assays to calculate the difference in absorbance (E1 and E2) after addition of two
reagents R1 and R2 to a sample. E1 represents the absorbance of a sample in addition with reagent 1 (R1) just
before a second reagent (R2) is added. E2 is the absorbance after adding R2. The course of the procedure is
outlined in Fig. 5.4.16.1.
The maximum number of samples depends on the length of the measure time of reagent 1 and 2 (T2 and T3).
The number of samples may be reduced by pushing [->R2] before starting the measurement or by skipping of
further samples during the first pipetting phase.
During the procedure the user is guided by indications on the screen (draw up and dispense reagent, etc., see
Fig. 5.4.16.2) and acoustic signals.
The factor Fdil is the volume correction factor which is calculated on the basis of the given volumes (sample volume
(a), R1 volume (b) and R2 volume (c) on page 3/3 of the method parameters). The factor is set to 1.000 by default
when given volumes are not set.
AS = E2 – Fdil * E1
whereby Fdil = (a + b) / (a + b + c)
Fig. 5.4.16.1: time controlled measuring
name of active method
D E L T A R 1 R 2
reading window Number of
0 S A M P L E S : 3 samples
F d i l : 1 . 0 0 0 0
Z [ A ]
T I M E 4 s Current time
dimension N E X T A C T I O N A T T I M E 5 s
Instructions
D I S P E N S E R E A : I N S A M P L E # 1 for next
action
action keys
E X I T M O D E - > R 2 Z E R O R E S U L T
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
info line
Fig. 5.4.16.2: screen during the method

5010e_73.docx / 23.04.19
Calculation procedure .................................................... CP 16

Characteristic ...................................................... DELTA R1R2
Method ..................... Difference measurement of two reagents
Calculation formula ...................................................... C = AS
Time / Delta T1........................................entering (20 to 255s)
Measure time R1 T2 ................................entering (0 to 1800s)
Measure time R2 T3 ................................entering (0 to 1800s)
Delay T4 ..................................................entering (0 to 1800s)

METHOD 20: DELTA R1R2
PROGRAM: 16
FACTOR: 1.000
WAVELENGTH: 546nm
TEMPERATURE: 37C The measuring window is shown.
TIME/DELTA: 30s
DELAY: 0s
TIME REA.#1 130s
TIME REA.#2 130s
UNIT:
MEASURE ZERO
→Insert / measure samples with reagent 1
E1 1 0.285 [A]
E1 2 0.285 [A]
E1 3 0.285 [A] →Insert / measure samples with reagent 2
E2 1 0.165 [A]
E2 2 0.165 [A] The user is guided through the measuring procedure on the
E2 3 0.165 [A] screen.
NO. RESULT
→The result will be displayed
1 -0.116
2 -0.116
3 -0.116

5010e_73.docx / 23.04.19
6 METHOD EDITOR
By the method editor the daily laboratory work can be substantially facilitated. Based on the 15 calculation
procedures up to 231 user-defined methods with their setting parameters can be saved. With the functions of the
editor a method can be established, changed or deleted.
Fig. 6.1
M E T H O D N E W / C H A N G E / C O P Y In the main window of the method editor
following options are available:
M E T H O D C O P Y L I S T [METHOD COPY] Change to Fig. 6.2,

where different copy functions can be
M E T H O D E D I T selected.
[METH. EDIT] Change to Fig. 6.3, where

M E T H O D N E W the number of the method to be edited is
queried. Afterwards all setting parameters of
M E T H O D D E L E T E E X I T the selected method can be changed.
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9 [METHOD NEW] Change to selection of

the calculation procedure (see 5.3 SURVEY
OF THE METHODS). In Fig. 6.4 all setting
Print-out of a method list: parameters can be edited.
METHOD 20: HEMOGLOBIN [METHODE DELETE] Change to Fig. 6.3,

F 29.4 405nm g/dl where the number of the method to be
deleted is queried. After a prompt for
METHOD 21: HDL-C confirmation the selected method is deleted.
F 1.000 546nm mg/dl (Basic and fixed methods cannot be
deleted).
METHOD 30: CREATININ
S 2.0 492nm mg/dl
[LIST] A list of all programmed methods
METHOD 31: GOT can be printed and transmitted via the serial
F–1746.0 340nm U/l interface.
[EXIT] Return to main menu
Fig. 6.2
[#→#] All methods from no 20 can be
copied to a new method no. First the method
to be copied is queried in Fig. 6.3. Its
M E T H O D C O P Y parameters can be changed starting with
Fig. 6.4.
[LAST] The method used last can be

copied on a new method place. Its setting
E X I T L A S T  →  parameters can be changed starting with
Fig. 6.4.
This function is very useful if a basic method
with new setting parameters was
successfully tested. These parameters can
be saved as a new method starting from no
20.
[EXIT] Return to main menu
ROBERT RIELE GmbH & Co KG Page 44 6 - METHOD EDITOR

5010e_73.docx / 23.04.19
Fig. 6.3
Inquiry window of the desired method.
The method used last is suggested. With [+]
M E T H O D N O . or [-] the methods can be scrolled. A numeric
input of the method number is possible at
M E T H O D : ( N A M E ) any time. A known method is indicated with
U N I T : ( D I M . )
name and dimension.
1 2 3 4 5 6 7 8 [E] Select shown method
E S C 9 0 . + - E [ESC] Return to main menu
Fig. 6.4 (parameter window 1)

M E T H O D E D I T R V ( x ) ( N A M E ) The parameter windows 1 and 2 show the
general method data.
1 - W A V E L E N G T H The parameter window 3 has special
2 - F A C T O R ( S T A N D A R D ) E X I T functions which are necessary for quality
3 - T E M P E R A T U R E
4 - D E L A Y control only (see below).
5 - U N I T O K
6 - M E A S . V O L U M E For each setting parameter a leading
7 - D E L T A ( I N C U B A T I O N )
8 - T I M E / D E L T A ( R E A C T I O N ) identification number is shown. If the
identification number is selected on the
keyboard, the corresponding setting
1 2 3 4 5 6 7 8 P 1 / 3 parameter becomes configurable.
Number and kind of setting parameters

depend on the calculation procedure. So
identification numbers can be occupied
Fig. 6.5 (parameter window 2)
variedly. Characteristic numbers without
M E T H O D E D I T R V ( x ) ( N A M E ) parameters do not have a function.
1 - M I N . V A L U E
2 - M A X . V A L U E E X I T [EXIT] Return to main menu
3 - M I N . R ^ 2
4 - M E T H O D N A M E [OK] Accept setting parameters (depending
5 - M U L T I M E A S U R E O K
6 - W A S H V O L U M E on editor mode sometimes with query of
7 - M U L T I - S T A N D A R D target method)
8 - B I C H R O M A T I C
[P../3] Change to next parameter window
1 2 3 4 5 6 7 8 P 2 / 3
Specifics in parameter window 3:

At least one control serum must be defined,
before data can be entered (see chapter
Fig. 6.6 (parameter window 3) 7.2.6 Quality control).
M E T H O D E D I T R V ( x ) ( N A M E )
If at least one control serum with its setpoint
and range is entered, corresponding
1 - I D S 1 memory of the quality control is reserved for
2 - M I N . V A L U E S 1 E X I T this method. So it can be supervised with
3 - R E Q U I R E D S 1
4 - M A X . V A L U E S 1 integrated quality control.
5 - I D S 2 O K
6 - M I N . V A L U E S 2
7 - R E Q U I R E D S 2 If both ID identifications are deleted,
8 - M A X . V A L U E S 2 then also all data and reserved memory of
this method in the quality control are
1 2 3 4 5 6 7 8 P 3 / 3 deleted!

5010e_73.docx / 23.04.19
Fig. 6.7
Query of the desired method number, under
which the new method is to be stored. The
next free method number is indicated. How-
M E T H O D N O . ever each free method number can be
N E X T F R E E N O . x x selected within the range of 20 to 250.
[E] Store method with selected number. In

1 2 3 4 5 6 7 8 case of multi-standard method Fig. 6.8
follows.
E S C 9 0 . + - E
[ESC] Break storage and return to editor
menu
Fig. 6.8 For a method with multi-standard there is

the editor window for the curve bases.
[P+] and [P-] Consecutive numbering of the

C U R V E N O . x x current bases
P 1 A - 0 0 0 0 0 N E W A
C - 0 0 0 0 0
[A/C] Switch input between A for
absorbance and C for concentration
1 2 3 4 5 6 7 8
[E] Accept the edited value
E S C 9 0 . P + P - A / C E
Input and confirmation of a single “0”

at A lead to the deletion of the current pair of
points. In order to set the value to zero enter
e.g. “0.0”.
[ESC] End input and save curve data
For measuring in a multi-standard

method at least 2 bases with A and C must
be defined!

5010e_73.docx / 23.04.19
7 UTILITY PROGRAMS
7.1 SELECTION OF UTILITY PROGRAMS
M A I N M E N U
Main menu:
Utility programs are necessary for the

M E A S U R E W I T H P R O G R . M E T H O D S adjustment and maintenance of the
photometer.
M E A S U R E W I T H B A S I C M E T H O D S
M E A S U R E N E W / C H A N G E / C O P Y
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
Page 1 of utility programs:

U T I L I T I E S P A G E 1 / 5
Scrolling through all utility programs is
O P T I C A D J U S T M E N T P A G E possible by [PAGE]. The current page is
shown at the right upper screen corner. By
M U L T I - S T A N D A R D [EXIT] the program returns to the main
menu.
P R I N T E R
A utility program is selected by pressing the
relating key.
P U M P M E N U E X I T
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
Utilities Description in
chapter
Optic adjustment 7.2.1

Multi-standard functions 7.2.2
Printer ON / OFF 7.2.3
Pump menu 7.2.4
Menu serial com 7.2.5
Quality control 7.2.6
Settings printout 7.2.7
Stored results 7.2.8
Temperature ON / OFF 7.2.9
Temperature adjustment 7.2.10
Laboratory name 7.2.11
User name 7.2.12
Error list 7.2.13
Key signal ON / OFF 7.2.14
Touchscreen adjustment 7.2.15
Date / Time 7.2.16
Language 7.2.17
ADC counts (Optic) 7.2.18
Bar Code 7.2.19
Service tools 7.2.20
ROBERT RIELE GmbH & Co KG Page 47 7- UTILITY PROGRAMS

5010e_73.docx / 23.04.19
7.2 DESCRIPTION OF UTILITY PROGRAMS

7.2.1 Optic adjustment
In case of working with flow-through
system:
O P T I C A D J U S T M E N T The optic adjustment should be done not

before the warm-up time of 15 minutes has
W I T H D I S T I L L E D W A T E R passed, better after one hour operation.
Aspirate distilled water by [sipping

E X I T S T A R T lever P] before starting the procedure.
The lid of the cuvette compartment

may stay open. The optic is not sensitive to
stray light.
Start the optic adjustment by [START].
In case of working with standard cuvette

adaptor:
O P T I C A D J U S T M E N T The optic adjustment should be done not

before the warm-up time of 15 minutes has
W I T H O U T C U V E T T E passed, better after one hour operation.
Remove any cuvette from the cuvette

E X I T S T A R T compartment.
The lid of the cuvette compartment

may stay open. The optic is not sensitive to
stray light.
Start the optic adjustment by [START].
Calibration procedure:
Wait for about 40s until adjustment is
D A R K A D J U S T M E N T finished.
8 3 9 2 1 9 6 The function cannot be interrupted. After
completion the program returns to the utility
P L E A S E W A I T program level.
Monthly executed the optic adjust-

ment compensates possible deviations of
the measuring accuracy due to
environmental influences.

5010e_73.docx / 23.04.19
7.2.2 Multi-standard functions

Before curve data of a method with multi-standard can be processed, the method must have been established in
the method editor (chapter 6 - METHOD EDITOR). A curve without corresponding method cannot be processed!
The term “curve no” has the same meaning as “method no”.
If using a multi-standard method later on, pay attention that all extinction values of the samples lie within the range
of the curve bases. Values outside of the extinction range cannot be calculated. In this case “+-“ is shown and
“<<< >>>” printed instead of the reading.
Fig. 7.2.2.1
Main window of multi-standard functions
U T I L I T I E S S U B M E N U E
[MEASURE CURVE] After query of a curve
M E A S U R E C U R V E L I S T number and the first standard the program
changes automatically to the method
P R I N T C U R V E selection window. There the given
parameters of the respective method can
be again controlled and/or changed. All
E D I T C U R V E
further standards are queried during the
following procedure.
S H O W C U R V E E X I T
The program for measuring the multi-
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9 standards branches out automatically to the
calculation procedures as follows:
CP1 → CP5
CP2 → CP6
CP3 → CP7
CP4 → CP8
CP9 → CP10
CP11 → CP12
CP14 → CP14
Print-out of curve:
For measuring in another standard
method than suggested, start measuring
multi-standards in each other selected
standard method (before measuring the first
standard) by [MODE] [MODE] [M-STD].
Curve number and standards are queried
accordingly.
[MEASURE CURVE] After inquiry of a

curve number start of measuring of standard
directly with a basic method which is based
on standard. This special mode ends
automatically after measuring or after
selection of a factor method. The standard
of the selected basic method represents the
first standard which can be measured. All
further standards are queried during the
measuring execution in the special mode.
[PRINT CURVE] After inquiry of the curve

number print-out on the internal printer or via
the serial interface.
For measuring in a multi-standard

method at least 2 bases with A and C must
be defined!

5010e_73.docx / 23.04.19
[EDIT CURVE] After inquiry of the curve

number all curve bases can be edited (see
fig. 7.2.2.2).
C U R V E [SHOW CURVE] After inquiry of the curve

N O . 2 0 number the function will be shown.
E X I T
[LIST] The method number and the date of

preparation of all current curves are shown.
[EXIT] Return to the utilities
Fig. 7.2.2.2 [P+] and [P-] Consecutive numbering of the

current bases
[A/C] Switch input between A for

C U R V E N O . x x absorbance and C for concentration
P 1 A - 0 0 0 0 0 N E W A
C - 0 0 0 0 0
[E] Accept the edited value
1 2 3 4 5 6 7 8
Input and confirmation of a single “0”

E S C 9 0 . P + P - A / C E at A lead to the deletion of the current pair
of points. In order to set the value to zero
enter e.g. “0.0”.
[ESC] End input and save curve data. The

bases are sorted in ascending order
according to their A value.
7.2.3 Printer ON / OFF
The current status of the internal printer is
indicated in the first line by OFF or ON.
P R I N T E R O F F Change setting by [SELECT]
Save setting permanently by [OK]
Save setting temporarily up to next switch-

E X I T O K S E L E C T off of the device by [EXIT]
NO. ABS. RESULT

- - - - - - - - - - - - When printer ON via [MODE] [PRN]
1 0.675 19.8
TIME: 11:21:32 after a measurement the current time can be
printed out by [TIME].

5010e_73.docx / 23.04.19
7.2.4 Pump menu

With these functions the pump and the bubble detector can be checked and adjusted. The check and adjustment
is possible only with inserted flow-through cuvette adaptor.
U T I L I T I E S S U B M E N U The menu offers following functions:
• Activation and deactivation of pump

P U M P O N / O F F
• Calibration of pump volume
P U M P C A L I B R A T I O N • Activation and deactivation of bubble
detector and of the volume optimized
P U M P S E T T I N G S mode.
• Media test: check and adjustment of
M E D I A T E S T E X I T bubble detector.
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
7.2.4.1 Pump ON / OFF

The status of the pump is indicated in the
first line by OFF or ON.
P U M P O N
Change setting by [SELECT]
E X I T O K S E L E C T Save setting temporarily up to next switch-

off of the device by [EXIT]
7.2.4.2 Pump calibration

The pump calibration can be executed with
or without bubble detector. So set bubble
P U M P C A L I B R A T I O N detector ON or OFF (see chapter 7.2.4.3.1).
E X I T W A S H
Behavior with bubble detector:

In preparation for the calibration first clean
the system by [WASH].
P U M P C A L I B R A T I O N
A I R 1 5 9 Pump exactly 1000 µl distilled water by
W A T E R 1 0 9 6 [Sipping lever P].
M S / 5 0 µ l 5 8
When the pump has stopped, values for air
E X I T O K W A S H and water are indicated which correspond to
the delivered volumes.
If the calibration is not possible,

although the pump tube is connected and
1000 µl were sucked in, execute a media
test (see chapter 7.2.4.4). Afterwards the
pump calibration can be repeated.

5010e_73.docx / 23.04.19
Behavior without bubble detector:

In preparation for the calibration first clean
the system by [WASH].
P U M P C A L I B R A T I O N Start to pump exactly 1000 µl distilled water
A I R 1 5 9 by [Sipping lever P]. With each operation of
W A T E R 1 0 9 6 [Sipping lever P] the pump wheel rotates
and delivers air. As soon as the liquid in the
M S / 5 0 µ l 5 8 tube can be seen between the metal inlet
E X I T O K W A S H tube and the screw connection of the
aspiration tube, confirm the event by [OK].
The turns are shown beside the entry AIR on
the screen and represent the air path.
During the second phase the rest of the

1000 µl distilled water needs to be pumped
by pushing [Sipping lever P] repeatedly.
The turn of the pump wheel is shown beside
the entry WATER. As soon as the end of the
liquid in the tube between the metal inlet
tube and the screw connection of the
aspiration tube can be seen, confirm the
event by [OK].
7.2.4.3 Pump settings

In this menu it is described how to configure
the bubble detector and the optimized
volume mode.
After having configured the bubble detector

the window for the configuration of the
optimized volume mode will be displayed
automatically.
7.2.4.3.1 Bubble detector ON / OFF

The current status of the bubble detector is
B - D E T . O N


5010e_73.docx / 23.04.19
7.2.4.3.2 Volume optimized ON / OFF

The status of this setting is indicated in the
first line by OFF or ON.
V O L U M E O P T I M I Z E D O N

off of the device by [EXIT].
When volume optimized is on, an acoustic

signal will be emitted after the required
volume is pumped. At this moment the
sample must be retired from the aspiration
tube. After a pause of approx. 1s the rest of
liquid in the tube will be completely pumped
into the flow through cuvette. For example,
this function makes it possible to pump two
consecutive times 500 µl from a 1000 µl
sample volume.
7.2.4.4 Media test

With the media test the bubble detector can
be checked and adjusted.
MEDIA TEST In the second line to the left of the medium
WATER the current sensitivity level of the
4 WATER
bubble detector is shown.
Sip distilled water by [Sipping lever P]. Be
1 2 3 4 5 6 7 8 sure that the aspiration tube is completely
filled with water.
ESC 9 0 . + - E Enter new sensitivity level step by step by
numeric key [0] up to [9], starting with 0, until
the display changes from “AIR” to “WATER”.
Then increase the sensitivity level by one
further step.
Empty the aspiration tube by [Sipping lever
P]. The display changes from “WATER” to
“AIR”.
The adjustment is permanently saved by [E].

5010e_73.docx / 23.04.19
7.2.5 Menu serial com

A PC or an external printer can be connected to Photometer 5010 via the RS 232 serial interface at the back. A
suitable data cable can be supplied (REF 501-002). The connected device must comply with safety standard
EN 60950.
U T I L I T I E S S U B M E N U The menu offers following functions:
• Activation and deactivation of EDP

E D P O N / O F F
• Activation of remote control
R E M O T E M O D E • Activation or deactivation of external
printer with serial interface
S E R I A L P R I N T E R • Setting of baud rate
B A U D R A T E E X I T
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
7.2.5.1 EDP ON / OFF

The status of the EDP (Electronic Data
Processing) interface is indicated in the first
E D P O F F line by OFF or ON.
Change setting by [SELECT]:
• EDP OFF: no output,

E X I T O K S E L E C T
• EDP ON (CR-LF): system output
through serial port with CR-LF protocol,
• EDP ON (STX-ETX-BCC): system
output through serial port with STX-
ETX-BCC protocol,
• EDP ON (CR-LF-LOG): after each
measurement a formatted string will be
output through the serial port. (e.g. see
table. 7.2.5.1)

Table 7.2.5.1
Serial Method ID-no. Sample Result Temperature User Date Time
no. no. no. control
11000 20 12345 1 15.5 * [user name] 09/08/09 09:30:47
7.2.5.2 REMOTE CONTROL

Activate remote control by [START].
R E M O T E M O D E When activated, Photometer 5010 can be
remote-controlled by a PC and a suitable
program.
Deactivate remote control by sipping lever

E X I T S T A R T [P].
By [EXIT] the program returns to the

superordinate menu item.

5010e_73.docx / 23.04.19
7.2.5.3 SERIAL PRINTER ON / OFF

The status of the external serial printer is
S E R I A L P R I N T E R O F F

7.2.5.4 BAUD RATE

The current baud rate is shown in the first
line.
B A U D R A T E 1 9 2 0 0
Change setting by [SELECT].
Save setting permanently by [OK].
E X I T O K S E L E C T Discard changes by [EXIT].
7.2.6 Quality control

In Photometer 5010 up to 50 methods can be supervised with a quality control. The device can manage up to 6
control serums. Each QC supervised method can be connected with 2 control serums. The QC data of a series
of measurements are stored in a daily memory. Each reading is stored with method number, date and user
identification. From the daily memory the individual QC data can become deleted or saved in the monthly memory
of the corresponding method. The monthly memory of a QC method can record up to 31 readings. With the 32nd
the oldest reading is deleted in the memory. For the calculation of the quality values of a method at least 20
readings in the monthly memory must be present. The average of all readings, the standard deviation and the
coefficient of variation are calculated. Contents of the daily and monthly memory can be indicated and printed out.
Except the basic methods all methods can be connected with a quality control. The method-typical data of a
control serum are entered via the method editor (see chapter 6 METHOD EDITOR).
U T I L I T I E S S U B M E N U E The QC menu offers following functions:
[EDIT CONTROL SERUM] Up to 6 control

E D I T C O N T R O L S E R U M serums can be defined. Without a defined
serum the QC cannot be started!
D A I L Y M E M O R Y
[DAILY MEMORY] View, print and process-
ing of the daily memory for serum 1/2
M O N T H L Y M E M O R Y
[MONTHLY MEMORY] View, print and
Q C E D P E X I T processing of the monthly memory for serum
1/2
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9
[QC EDP] - not implemented -

5010e_73.docx / 23.04.19
C O N T R O L S E R U M 1 7.2.6.1 INPUT OF CONTROL SERUM
1 - I D [1] Enter name max 15-digit

2 - E X I T
3 - L O T
4 - [3] Enter LOT no max 10-digit
5 - C O M P A N Y O K
6 - [5] Enter company max 10-digit
7 - D A T E
8 -
[7] Enter expiry date max 8-digit
1 2 3 4 5 6 7 8 N O . [NO.] Change to next control serum
3 7 . 0 ° C 0 1 / 1 3 / 1 5 1 1 : 3 9 [EXIT] and [OK] Accept input and return to

QC menu
7.2.6.2 DAILY MEMORY
[S1] Select daily memory for serum 1

S E L E C T M E M O R Y
[S2] Select daily memory for serum 2
[EXIT] Return to previous window
E X I T S 1 S 2
Measuring data of the corresponding daily

memory are shown with method number,
D A I L Y M E M O R Y S 1 method name, reading and dimension.
+
2 5 G L U C O S E [+] Change to next reading
1 3 . 0 3 m m o l / l - [-] Change to previous reading
E X I T D E L . S T O R E P R I N T [DEL] Delete shown reading in the daily

memory and confirm again by [DEL]
[STORE] Store shown reading in the

monthly memory and confirm again by
[STORE]. Afterwards the reading is deleted
in the daily memory.
Print-out of daily memory for serum 1:
[PRINT] Print all readings
*** DAILY MEMORY ***S1 *
PHOTOMETER 5010 #20001 [EXIT] Return to QC menu
V7.1a dd/mm/yy D
LAB.: RIELE BERLIN
DATE: 09/03/17
TIME: 08:44:12
25 GLUCOSE 13.03
21 HDL-C 367
27 UREA COL 197.2
29 CK-MB 1128.1
31 GOT 189.9

5010e_73.docx / 23.04.19
7.2.6.3 MONTHLY MEMORY

After query of method number select serum
1 or 2 of the method.
C O N T R O L S E R U M
[S1] Select monthly memory for serum 1
[S2] Select monthly memory for serum 2
E X I T S 1 S 2 [EXIT] Return to previous window
M O N T H L Y M E M O R Y S 2
In the overview window of the selected
M E T H O D 2 5 G L U C O S E method all data of quality control are visible.
S E R U M 1 L T - S Y S a b n o r m a l In the line above the keys following
L O T G 3 1 2 information the current reading is indicated:
C O M P A N Y L A B O R + T E C H
D A T E M A Y 0 6
R E Q U I R E D 1 4 . 4 m m o l / l (# 1) → Numerator of the monthly memory.
R A N G E L : 1 2 . 1 O : 1 6 . 7 The oldest reading corresponds to the 1.
C A L C U L . s : 1 . 0 1 2 C V : 7 . 2 9 8
m : 1 3 . 8 6 0 n : 2 0
(01.27.06) → Date of reading
 1 0 7 . 0 9 . 0 7 1 3 . 3 0 - 1 s 1
E X I T M O R E D E L E T E - + (13.30) → Reading
(-1.s) → Deviation of the reading lies within

minus 1s. From +/-3s the warning level
starts. With a deviation of > 3s an * is
displayed. For the calculation of the quality
values of a method at least 20 readings in
the month memory must be present!
(1) → User identification
The keys have following functions:
[+] Change to next reading
[-] Change to previous reading
[DEL] Delete all measuring data of the

monthly memory of the selected method and
confirm again (e.g. at change of serum)
[MORE] Change to output dialog
[EXIT] Return to QC menu

5010e_73.docx / 23.04.19
Output dialog
If at least 20 readings are stored in the
P R N - N monthly memory, these are indicated in the
Levey Jennings plot. In this representation
the deviations can be controlled visually and
P R N - E
thus tendencies or systematic errors be
better recognized.
The keys for the printout are located next to

E X I T the curve diagram:
[PRN-N] Start the normal printout of the

data of the current monthly memory. The
single data of the readings are not printed
Printout of the monthly memory of a method with serum 2: thereby.
** MONTHLY MEMORY **S2 * [PRN-E] Start the extended printout of the

DATE: 09/03/17 data of the current monthly memory. As
PHOTOMETER 5010 #20001 shown in the example left, also the single
V7.1a dd/mm/yy D data of the readings are printed.
LAB.: RIELE BERLIN
METHOD 25: GLUCOSE [EXIT] Return to QC menu
UNIT: mmol/l
SERUM NO. 5
ID LT-SYS abnormal
LOT G312
COMPANY LABOR+TECH
DATE MAY 11
REQUIRED 14.4
MIN.VALUE 12.1
MAX.VALUE 16.7
QC VALUES n: 20
MEAN m: 13.860
STD.DEVIATION s: 1.012
COEFF.OF VAR CV: 7.298
02/15/10 13.54 -1s 1

02/14/10 14.07 +1s 1
02/13/10 14.69 +1s 1
02/12/10 13.50 -1s 3
02/11/10 14.68 +1s 3
02/10/10 15.33 +2s 1
02/09/10 15.99 +3s 1
02/08/10 15.38 +2s 2
02/07/10 14.61 +1s 1
02/06/10 13.70 -1s 1
02/05/10 12.74 -2s 1
02/04/10 12.13 -2s 1
02/03/10 12.65 -2s 2
02/02/10 13.11 -1s 1
02/01/10 13.88 +1s 3
01/31/10 13.51 -1s 3
01/30/10 13.24 -1s 3
01/29/10 12.50 -2s 1
01/28/10 12.74 -2s 2
01/27/10 13.30 -1s 1

5010e_73.docx / 23.04.19
7.2.6.4 QC EDP
Not implemented
7.2.7 Settings printout

By [START] the program version and the
complete status of the saved settings are
printed out.
S E T T I N G S P R I N T O U T
E X I T S T A R T
******ACTUAL*SETS*******
DATE: 09/03/17
TIME: 08:44:12
PHOTOMETER 5010 #20001
V7.1a dd/mm/yy D
/T1.7
CORE V1.0
PCB LAYOUT c
ADC COUNTS (DARK ADJ.
S: 8394148 A: 8392514
FILTER
1: 340 2: 405 3: 492 The percentage is proportional to the
21/21% 18/23% 12/18%
4: 546 5: 578 6: 623 brightness level.
13/20% 17/26% 16/24% The percentage value left represents the S
7: 999 8: 999 9: 999 adapter and the right one the A adapter.
0/ 0% 0/ 0% 0/ 0%
TEMPERATURE S
25C 8939 3000
30C 10165 3000
37C 11874 3000
TEMPERATURE A
25C 8687 3000
30C 9892 3000
37C 11601 3000
PUMP
MS AIR 178
MS/50µl 56
PUMP SPEED (1-30) 25
B-DET. 6
VOLUME OPTIMIZED OFF
BATTERY: OK
ADC CORRECTION 250
EDP ON (CR-LF)
TOUCH
Mx148 My192 Fx120 Fy100
LANGUAGE
1: ENGLISH
2: GERMAN
KEY SIGNAL ON
T.COUNTER : 334:45
S.COUNTER : 402
PROGR. METHODS 0
STORED RESULTS 123

5010e_73.docx / 23.04.19
7.2.8 Stored results

[EXIT] terminates the program.
[SHOW] indicates stored data step by step.

S T O R E D R E S U L T S
A C T U A L 5 [SEND] transmits stored results through the

serial port.
After transmitting is completed you will be

E X I T S H O W S E N D prompted to delete stored results. Press
[START] to delete results or [EXIT] to exit
without deleting results.
7.2.9 Temperature ON / OFF

The status of the tempering is indicated in
the first line by OFF or ON. Besides the
inserted cuvette adaptor is marked by A or
T E M P E R A T U R E O N A S.
O F F
2 5 C Change setting by [SELECT]. Following
3 0 C options are possible:
3 7 C
E X I T O K S E L E C T • OFF
• 25° C
• 30° C
• 37° C

off of the device by [EXIT].
7.2.10 Temperature adjustment
The temperature control was

T E M P . A D J U S T M E N T
adjusted at the factory!
Anyhow the tempering control can be cali-
T E M P . A C T U A L : 3 6 . 9 0 brated provided that the tempering was
switched on for at least 30 minutes:
1 2 3 4 5 6 7 8 Measure the current temperature with an
independent measuring system (e.g.
thermistor, REF 090-063) inside the cuvette
E S C 9 0 . + - E
and enter this value. According to the
difference to 25.0 °C, 30.0 °C or 37.0 °C the
system corrects its internal setting. The
calibrating of the temperature is interrupted
when the tempering is off or the temperature
unstable.
Enter the password “5010”.
Enter the actual temperature in TEMP.

ACTUAL four-digit in °C (e.g. 36.90) and
confirm by [E].

5010e_73.docx / 23.04.19
7.2.11 Laboratory name

The name of the laboratory can be stored
permanently.
L A B O R A T O R Y N A M E In case of a stored name an additional line

within the header is sent to the printer or to
the EDP.
By [NEW] the entry of the laboratory name is

possible.
E X I T N E W
U T I L I T I E S P A G E 3 / 5 Enter the laboratory name via the alphanu-

meric keyboard.
Following functions are available:
N E W L A B O R A T O R Y N A M E
< • [a/1] : change to lowercase

• [1/A] : change to numeric characters
Q W E R T Y U I O P
• [A/a] : change to uppercase
A S D F G H J K L  • [] : delete character

• [→] : blank
E S C Z X C V B N M A / 1 E N T
• [ESC] : finish input without storage
• [ENT] : finish input with storage
7.2.12 User name

The names of maximum five users can be
stored permanently.
U S E R N A M E After calling a method the user is queried.
1 :
2 :
3 : In case of a stored name an additional line
4 : within the header is sent to the printer or to
5 :
the EDP.
E X I T N E W S E L E C T
Select user by [SELECT]. By [NEW] the
entry of the user name is possible.
U T I L I T I E S P A G E 3 / 5 Enter the user name via the alphanumeric

keyboard.
Following functions are available:
N E W U S E R N A M E 1
• [a/1] : change to lowercase
<
• [1/A] : change to numeric characters
Q W E R T Y U I O P
• [A/a] : change to uppercase
A S D F G H J K L  • [] : delete character

• [→] : blank
E S C Z X C V B N M A / 1 E N T
• [ESC] : finish input without storage
• [ENT] : finish input with storage

5010e_73.docx / 23.04.19
7.2.13 Error list

The last 10 serious errors are shown or
printed.
E R R O R L I S T The oldest error is shown first. The last error

N O . 1 is always marked with no 1.
D A T E 0 9 / 0 8 / 0 9
E R R O R C O D E 4 6 By [NEXT] earlier error messages are
shown.
E X I T P R I N T N E X T
By [PRINT] the complete error list is printed
or output to the serial interface.
For troubleshooting the coded error list can

be consulted (chapter 9.4 - CODED
ERROR MESSAGES).
7.2.14 Key signal ON / OFF

The current status of the key signal is indi-
cated in the first line by OFF or ON.
K E Y S I G N A L O N Change setting by [SELECT].

E X I T O K S E L E C T off of the device by [EXIT].
The deeper signal tone for error messages

remains active in any case.
7.2.15 Touchscreen adjustment
X 0 Y 0 By this function the touchscreen can be

adjusted. After call of the function a white
cross is shown in the left lower corner of the
T O U C H S C R E E N A D J U S T M E N T screen. Touch the intersection point in the
cross with a non-scratching plastic tip
(touchscreen pen, pipette tip) as exactly as
P R E S S E X A C T L Y T H E C R O S S P O I N T
possible. In the first line the coordinates are
shown as X- and Y-value. The input will be
T I M E 1 0 accepted and the coordinate display will be
reset after a time out of 10s. Then the cross
is shown in the right upper corner. Touch the
intersection point. After a timeout of 10s the
memory inquiry follows.
Save the adjustment by [OK].
Reject the adjustment by [EXIT].
S A V E T S A D J U S T M E N T
E X I T O K

5010e_73.docx / 23.04.19
Hint: If the device is maladjusted, this function can directly be called during the switching on routine:
Switch on the device. After greeting screen (chapter 2.3 - INSTALLATION) is displayed, keep the touchscreen or
the zipping lever [P] pressed. After some seconds a deep signal tone sounds and the text message
“TOUCHSCREEN ADJUSTMENT” will be shown at the first line of the greeting screen. Release the touchscreen
within one second. Execute the adjustment of the touchscreen as described above. Select the type of photometer
after touchscreen adjustment is completed.
Select type of photometer:
Reject setting by [EXIT].

S E L E C T P H O T O M E T E R
P H O T O M E T E R 5 0 1 0 Save setting permanently by [OK].
With [SELECT] the type of photometer is

selected.
7.2.16 Date / Time

The status of the date/time display is
D A T E / T I M E O N Change setting by [SELECT].
With activation of the clock date and time

can be changed by [OK]. Each entry of day,
DATE TIME month, year, minute and second must be
mm/dd/yy hh:mm:ss confirmed by [E].
10/27/10 15:35:00
If a value is to be changed, then all
values must be entered again!
1 2 3 4 5 6 7 8
ESC 9 0 . + - E
NO. ABS. RESULT

- - - - - - - - - - - - When printer ON via [MODE] [PRN]
1 0.675 19.8
TIME: 11:21:32 after a measurement the current time can be
printed out by [TIME].

5010e_73.docx / 23.04.19
7.2.17 Language
The status of the language is indicated in the
first line.
The setting can be changed by [SELECT]

L A N G U A G E 1 E N G L I S H Following options are possible:
• LANGUAGE : ENGLISH
E X I T O K S E L E C T • LANGUAGE : GERMAN
The setting is temporarily saved up to next

switch-off of the device by [EXIT].
With LANGUAGE 6 DOWNLOAD, three

additional languages can be added with
[OK] using the download program.
If you select START DOWNLOAD [YES], all

the old languages 3 – 5 are deleted and
exchanged with the new set of languages.
7.2.18 ADC counts (Optic)
U T I L I T I E S P A G E 5 / 5 Indicated is the current value of the optical

analogue-digital converter. The value is
proportional to the light-current.
A D C C O U N T S ( O P T I C )
F 2 4 0 5 n m 7 6 3 9 4 6 8 (A) value
S 0 7 5 2 8 5 1 (B) value = Dark level – (A) value
L .
To a key actuation the system reacts
possibly only after three seconds.
F 0 F 1 F 2 F 3 F 4 F 5 F 6 F 7 F 8 F 9
The functions F0 to F9 place the filter wheel
E S C into the positions 0 to 9. The position 0 corre-
sponds to the filter wheel position at the dark
level adjustment.
The function [L] switches the LED off and
on.
Stop function by [ESC].

7.2.19 Bar Code
The status of the bar code is indicated in
the first line.
The bar code reader can be activated by
B A R C O D E O N changing the setting by [SELECT].
The settings of the barcode reader

must be set to: RS232, CR/LF,
XON/XOFF, 19200 baudrate, 8 Bit, 1
Stop, non parity, XON/XOFF setting.
When a bar code is inserted it is displayed

instead of an ID-NO on the display, printout
and in the stored results.
Stop function by [EXIT].

5010e_73.docx / 23.04.19
7.2.20 Service tools

The service tools are reserved for trained
specialists only and therefore protected by a
password.
S E R V I C E T O O L S
Stop function by [ESC].
P A S S W O R D
1 2 3 4 5 6 7 8
E S C 9 0 . + - E

5010e_73.docx / 23.04.19
8 MAINTENANCE
This chapter provides necessary information concerning general maintenance by the user.
If any faults should occur which cannot be remedied, then service should be contacted. Repairs at the
device may be carried out only by authorized specialist staff. Through improper repairs the warranty extinguishes,
and the user can be heavily jeopardized.
8.1 CLEANING INSTRUCTION
Liquid waste is potentially biologically hazardous. Always wear gloves if handling those materials. Do not
touch parts of the device other than those specified. Consult the laboratory protocol for handling biohazardous
materials.
Take care that no liquid enters the device! There is no protection against penetrating of liquids
(Code IP X0). Check from time to time that the tubing and the connections are leakproof.
The flow-through system of the Photometer 5010 has to be washed with dist. water regularly before and after
measuring 2 – 3 times. Depending on sample material and reagent, and always at the end of a working day, the
Photometer 5010 has to be cleaned additionally with a phosphate-free detergent, e.g. with approx. 5ml of Biogent-
A (REF 5010-024), and afterwards rinsed with 5ml distilled water.
Persistent residues are to be removed with a combined alkaline-acid treatment. The following procedure is
advised step by step:
1. 5 x wash with NaOH 1 N
2. 5 x rinse with dist. water
3. 5 x wash with HCl 0.5 N
If the system is contaminated severely, it may alternatively be cleaned with hypochlorite. The following procedure
is advised step by step:
2. 2 – 3 x wash with hypochlorite (1:20 diluted solution) or ISE Cleaning Solution,
undiluted, let it possibly affect up to 20 minutes
3. 5-10 x rinse with dist. water
For device cleaning and surface decontamination purposes use commercial decontaminating solution which are
usually available in clinical chemistry laboratories like Mikrozid ® AF Liquid, Bacillol® plus, 3 % Kohrsolin® or similar
solutions. Switch off the device and disconnect it from the mains voltage. Then clean the device with soft cloth
and decontaminating solution.
Empty the drain tank at the end of daily measurement, or whenever filled.
8.2 CALIBRATING MEASURING SYSTEM

At doubtful measurement results an optic adjustment has to be carried out corresponding to chapter 7.2.1.
8.3 ADJUSTMENT OF BUBBLE DETECTOR

See chapter 7.2.4 - Pump
8.4 CALIBRATION OF PERISTALTIC PUMP

See chapter 7.2.4 - Pump
8.5 REPLACEMENT OF PAPER ROLL

See chapter 2.4 - LOADING PRINTER PAPER
ROBERT RIELE GmbH & Co KG Page 66 8 - MAINTENANCE

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8.6 REPLACEMENT OF ASPIRATION TUBE

When replacing the aspiration tube pay attention to the sequence of the assembly parts as shown below. Then
put aspiration tube without bending from the cuvette area side through the metal inlet tube. Turn screw connection
with fingers into the cuvette.
inlet tube with base
screw connection, milled O-ring washer
8.7 REPLACEMENT OF LINE FUSES

The Photometer 5010 operates at any line voltage between 100 VAC and 240 VAC at 50/60 Hz without adjustment.
It has two line fuses in series with the power supply. They are located on the rear panel. To replace those fuses,
unplug the mains cable and remove the fuse holder with the fuses as shown below. The instrument is delivered
with two spare fuses.
Neither use makeshift fuses nor short-circuit the fuse holder!

Specifications of mains fuse:
• dimensions [mm] : 5 * 20
• standard: IEC 60127-2/V
• time-current characteristic: time lag (T)
• voltage rating: 250 V
• rated current: 1.6 A
• marking: T 1.6 A H
mains switch
fuse holder
mains cable connector
ROBERT RIELE GmbH & Co KG Page 67 8 - MAINTENANCE

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9 ERROR MESSAGE / CORRECTION
9.1 GENERAL NOTE

Faulty input (e.g. wrong method number or wrong factor), recognized by the user, can be corrected by filling up
the respective entry field with any signs. After replenishing beyond the last position the faulty input is deleted and
the entry field is free again for the renewed correct input.
Error messages by the device are carried out either exclusively via a signal tone (chapter 9.2 - ACOUSTIC
ERROR MESSAGES) or as combination signal tone and display.
In the display errors are shown as plaintext (chapter 9.3 - PLAINTEXT ERROR MESSAGES)
… or coded with an error number (chapter 9.4 - CODED ERROR MESSAGES).
Each error message has always to be confirmed with [E].
9.2 ACOUSTIC ERROR MESSAGES

When pressing a key which is not permitted or not meaningful a deeper signal tone still sounds as error message
after the higher signal tone (which is to confirm the keystroke, can be switched off according to chapter 7.2.14 -
Key signal ON / OFF). In the display no corresponding error message appears parallel to this. The operation of
the device can directly be continued by the correct keyboard entry.
9.3 PLAINTEXT ERROR MESSAGES
RANGE MIN. The programmed low limit was under-run by the measurement.
RANGE MAX. The programmed upper limit was exceeded by the measurement.
NON-LINEAR The square of the correlation coefficient R lies at the kinetic measuring below the
programmed low limit.
RANGE +/- At the kinetic measuring the procedure of the kinetic is wrong (increasing / falling).
NO METHOD Dialed method is not programmed. Select other method according to method list.
HEATING OFF Heating / cooling is off during temperature calibration.
TEMP. UNSTABLE Temperature is unstable during temperature calibration.
9.4 CODED ERROR MESSAGES
No. (possible) Causes Remedy
1 method is write protected, method cannot be cleared by special software

2 check sum of a freely programmed method is wrong program new method
3 forbidden input, wrong number format repeat input in permitted area
4 method not available method editor:
check method no.
5 dark value is absolutely too high (> 16 bit) or higher as the repeat optic adjustment (chapter 7.2.1);
measurement, check LED / filter; check blank
ADC overflow
6 all multiplexer positions are too bright/too dark at setting to repeat optic adjustment (chapter 7.2.1);
zero check filter / LED; check blank
7 mathematical overflow, at measurement calculation check filter; check standard;
check measuring solution
8 check sum error in the data record of the dark offset repeat optic adjustment (chapter 7.2.1)
ROBERT RIELE GmbH & Co KG Page 68 9 - ERROR MESSAGE / CORRECTION

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9 check sum error in the data record of the device basic automatic error remedy
setting (status, ADC correction)
10 division by a too small value (< 0.001 A) check filter; check standard;
check measuring solution
11 invalid calibration curve Select valid number
12 setting to zero not possible (zero value is < 32768 cycle) check LED; check filter;
check zero solution
13 setting to zero not possible (zero value is > 983039 cycle) repeat optic adjustment (chapter 7.2.1);
check LED; check filter;
check zero solution
14 invalid standard measure valid standard solution
15 no parameter memory vacant (too little memory for non- delete a no longer actual nonlinear
linear methods) method
16 method no. is occupied select other method no.; delete a no
longer actual nonlinear method
17 check sum error in the parameter memory (nonlinear program method newly
method)
18 at calculation overflows in nonlinear method check factor; check parameter
19 clock malfunction
20 overflow at Kinetic check measuring solution
29 wrong input of deltas or time per delta restart method
30 battery empty contact service partner
31 communication: wrong data format contact service partner
32 communication: sent data not plausible / not interpretable contact service partner
33 communication: mentioned module does not answer in a check interconnecting cable;
certain time check mentioned module
34 communication: overflow send/receive buffer reduce amount of data at the
communication partner
35 remote control: wrong method number external software problem
36 remote control: unknown command external software problem
37 remote control: wrong data format external software problem
38 check sum of operating system bank 0 damaged contact service partner
39 check sum of operating system bank 1 damaged contact service partner
40 timeout at reception from module 2 switch off/on device
41 check sum error at reception from module 2 switch off/on device
42 NAK at reception from module 2 switch off/on device

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44 error at pump calibration - clamp pump tube;

- pump tube not clamped; - control volume of aspiration;
- no liquid aspirated; - control connectors of tubes;
- too much liquid aspirated during calibrating; - the plug of the bubble detector has a bad
- power of the pump too small; contact;
- motor does not rotate or stops; - the cable of the bubble detector has a
- connectors of tubes leaky; bad contact;
- bubble detector works falsely; - adjust bubble detector (chapter 7.2.4);
- aspiration tube blocked; - replace aspiration tube (chapter 8.6)
- aspiration tube badly stained
45 error at sipping solution - clamp pump tube;

- pump tube not clamped; - adjust bubble detector (chapter 7.2.4);
- no liquid aspirated; - control volume of aspiration;
- power of the pump too small; - control connectors of tubes;
- motor does not rotate or stops; - do a calibration of peristaltic pump
- connectors of tubes leaky; (chapter 7.2.4);
- bubble detector works falsely; - the plug of the bubble detector has a bad
- aspiration tube blocked; contact;
- aspiration tube badly stained - the cable of the bubble detector has a
bad contact;
- replace aspiration tube (chapter 8.6)
46 filter position out of tolerance contact service partner
51 Wrong adaptor insert standard cuvette adaptor
(chapter3.5.1)
52 timeout printer internal the internal printer is temporarily discon-
nected
53 Set of data points is missing check multi-standard functions
55 number of given data points < 2 add data points
56 pump calibration not successful repeat pump calibration using exactly
1000 µl (chapter 7.2.4)
59 error in the automatic measuring operation (remote) check interface
60 error at multiplexer of operational amplifier contact service partner
61 error at multiplexer of bubble detector contact service partner
62 free method number not found check method memory
63 wrong address at multi-standard loading check multi-standards
64 current method not found in monthly memory check QC data of method
65 more than 50 QC methods defined delete unused QC methods
66 internal clock is off. QC data not storable switch on internal clock
67 BCC error in dataset of QC method values check current method
68 free monthly memory not found at QC delete unused QC methods
69 free space in daily memory not found at QC empty QC daily memory
70 error at QC calculation check QC data
71 QC serum not found check QC data
72 wrong address at saving test results send results through serial interface and
delete results (chapter 7.2.8)
73 results memory full, it will be overwritten overwrite results memory or send and
74 BCC error at sending result string send results through serial interface and
87 Concentration too low Check sample
88 Concentration too high Check sample
90 At startup error in the text area Languages 3 - 5 are deleted

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10 TECHNICAL DATA
10.1 ENVIRONMENTAL CONDITIONS

Climatic conditions for storage and transport of the packed device:
• Temperature: -25 °C to +70 °C
• Relative humidity: 20 % to 85 %
•
The Photometer 5010 must be used in an environment that meets the following conditions:
• Temperature: +15 °C to +35 °C
• Relative humidity: 20 % to 85 %
• Not exposed to direct sunlight or other source of direct light (e.g. a spot light)
• Well-ventilated area
• Free from excessive dust
• Free from combustible gases
• Free from vibrations
• Free from electromagnetic wave interference
• Well-distanced from a machine generating a high frequency high voltage (e.g. a centrifuge)
10.2 MINIMAL OPERATION QUALITY

Signal processing in analogue amplifiers with high amplification factors cannot differentiate desired from
undesired signals. Amplifiers thus are apt to be overloaded or produce spurious signals. The equipment will
operate correctly when the undesired signals are removed. Short-term changes of the operational behavior do
not influence the overall function of the device.
10.3 TYPE PLATE

For installation pay attention to the specifications on the type plate.
ROBERT RIELE GmbH & Co KG Page 71 10 - TECHNICAL DATA

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10.4 SHORT SPECIFICATIONS

Type Semi-automatic, single-beam filter photometer
Light Source LED with long lifetime
Wavelength 340 nm and 390 nm – 730 nm
Wavelength Selection Automatic via 9-position filter wheel :
6 standard interference filters: 340 nm, 405 nm, 492 nm, 546 nm, 578 nm
and 623 nm; 3 positions for optional filter of choice
Photometric Range 0.0 – 2.5 A
Cuvette System Micro flow cell:32 l, 10 mm light path interchangeable with normal standard
cuvettes (macro or semi-micro, disposable or special optical glass)
Temperature Control ▪ Internal Peltier element, temperature variable, pre-adjusted to 25 °C,
30 °C and 37 °C
▪ Equilibration time for aspirated reaction mixture to reach 37 °C from
ambient temperature: 15 s
Aspiration System Built-in peristaltic pump driven by stepper motor, programmable
aspiration volume controlled by infrared light barrier
Sipping Volume ▪ Minimum 250 l, typically 500 l up to 2000 l
▪ Separate setting of aspirate volume and wash volume
Operator Interface Touchscreen for direct functions and alphanumerical inputs
Data Presentation Graphic display: White characters or symbols, blue background, lighted,
resolution 240 * 128 dots
Integrated Printer Thermal printer
Languages English and French/German/Indonesian/Russian/Spanish
Memory ▪ General operating software can be updated by PC
▪ Reagent-open system with capacity for up to 231 programmable methods
▪ Data import by touchscreen or PC
▪ Up to 50 nonlinear calibration curves with max 20 sets of points can be
stored
Signal Port Serial port for connection to an external printer and/or PC
Data Logging Up to 1000 results can be saved in the memory automatically
Measurement ▪ Absorbance
Procedures ▪ End point with factor, standard or multiple standards,
with or without reagent blank and/or sample blank
▪ Bichromatic end point
▪ Kinetics with factor, standard or multiple standards, with or without blank
▪ Fixed time with factor, standard or multiple standards,
with or without reagent blank
▪ Turbidimetry with optional timer function
▪ Single, double and triple determinations
▪ Curve fitting for nonlinear standard curves
▪ Free hemoglobin in combination with optional interference filters
Quality Control Up to 50 methods can be controlled with two control serums,
Levey Jennings plot
Measuring Time ▪ Kinetic: variable from 3 – 19 deltas, time per delta 3 – 255 s
▪ Fixed time: variable from 0 – 1800 s
Delay Time Programmable from 0 – 1800 s
Mains Supply Range: 100 VAC up to 240 VAC at 50/60 Hz
Dimensions Length 33 cm x width 34 cm x height 18 cm
Weight 5.0 kg
Marking

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10.5 TECHNICAL SPECIFICATIONS

Description according to DIN 58960 part 4
A Identification
A.1 Type of photometer: Photometer 5010

A.2 Model: 5010
A.3 Instruction for use: Photometer 5010, user manual
A.4 Manufacturer ROBERT RIELE GmbH & Co KG

Kurfuerstenstrasse 75-79
D-13467 Berlin
Germany
DECLARATION OF CONFORMITY:
The above mentioned absorption photometer is in conformity with the following metrological description.
Berlin, April 2019
W. Riele
B Metrological description
B.1 Measuring System

B.1.1 Optical configuration: cf. Figure
B.1.2 Source[s] of radiation: LED
B.1.3 Spectroscopic apparatus: filter wheel
B.1.4 Radiation detector[s]: photodiode
B.1.5 Cuvette[s]/cell[s]: 10 mm glass or plastic cuvette (square
shaped) or 10 mm flow-through cuvette
B.1.6 Temperature regulation of the cuvette: 25 °C, 30 °C or 37 °C
B.1.7 Displayed units: absorbance, mass concentration,
enzyme activity
B.1.8 Display device: digital display,
absorbance: 0.000 to 2.500
mass concentration: 0.000 to 9999
enzyme activity: 0.000 to 9999
B.2 Method of measurement

B.2.1 Generation of spectral absorbance A() monochromatic measurement
B.2.2 Zero compensation of spectral absorbance manual
B.2.3 Control of the measured spectral absorbance: with an absorption reference
solution (see manual)
B.2.4 Determination[s] of concentration: Lambert-Beer-Equation

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B.3 Specified measuring range

Outside the specified measuring range and under rated operating conditions other than those
stated in section B.4, the maximum permissible errors given in section B.5 can be exceeded.
B.3.1 Spectral absorbance A(): 0.0 A to 2.5 A

B.3.2 Wavelength  useable for measurements: 340 nm and 390 nm to 730 nm
B.4 Specified Operation conditions

B.4.1 Spectral transmittance of the cuvette: > 75 %
B.4.2 Warm-up time: 15 min
B.4.3 Operating voltage [mains]: between 100 VAC and 240 VAC at 50/60 Hz
with a tolerance of 10 %
B.4.4 Ambient temperature: 15 °C to 35 °C
B.4.5 Sound pressure level SPL < 50 dB
B.5 Maximum permissible errors and other limiting values

B.5.1 Photometric uncertainty of the spectral absorbance: max  3 %
B.5.2 Photometric short-time variation coefficient: 1%
B.5.3 Uncertainty of wavelengths: max  2 nm
B.5.4 Spectral half-width of spectral radiation flux at
detector:  10 nm
B.5.5 Percentage of wavelength integrated false
radiation ( measured at 340 nm as transmittance
of a cut-off filter NaNO3):  0.1 %
B.5.6 Uncertainty of temperature regulation:  0.2 °C
B.5.7 Specimen related carry-over ratio:
measure according to Broughton with
p-Nitro phenol 1%
OPTIC CONSTRUCTION
The optical path is directed from left to right of the device. Insert standard cuvette accordingly.
Diaphragm
Lens 1 Lens 2
Cuvette Photodiode
LED
Cuvette adaptor Filter

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11 ACCESSORIES AND SPARE PARTS
Kindly contact directly the responsible dealer.

REF Description
5010-065 Aspiration tubing 185 mm, 2 pcs
5010-024 Biogent-A, 1000 ml
1706870001 Bubble detector with plug
573655001 Cuvettes of optical glass, 4 pcs
501-002 Data cable serial interface
805-410 Disposable cuvettes, 1000 pcs
1704818001 Dust cover
5010-201 Flow through cuvette adaptor V7
5010-018 Fuses for line power, 10 pcs
500-002 Incubator T12
500-001 Incubator T16
1707175001 Joint inlet tube cuvette
0552402001 Mains cable
5010-005 Operator’s manual
5010-066 Outlet tube cuvette, 5 pcs
090073 Thermal printer paper, 5 rolls
090075 Thermal printer paper, permanent 10 years, 5 rolls
5010-050 Pump tube with joints
090-064 Secondary calibration standards, four-piece, certified
5010-202 Standard cuvette adaptor V7
1707574001 Top cover small for printer
1704834001 Waste tubing, 2 pcs
Incubator T12 Incubator T16
ROBERT RIELE GmbH & Co KG Page 75 11 - ACCESSORIES AND SPARE PARTS

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12 METHOD LIST
1 - 16 .......... 16 basic methods (chapter 12.1 - BASIC METHOD)

17 - 19 ........ free (reserved for further 3 automatic calculation methods)
20 - 250 ...... up to 231 user specific methods (chapter 12.2 - LIST OF USER SPECIFIC METHODS as copy
master / to be filled out by the user)
12.1 BASIC METHODS
ROBERT RIELE GmbH & Co KG Page 76 12 - METHOD LIST

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12.2 LIST OF USER SPECIFIC METHODS
ROBERT RIELE GmbH & Co KG Page 77 12 - METHOD LIST

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Operating Instructions

Photometer 5010 V5+

ROBERT RIELE GmbH & Co KG

Software Version 7.3

This product fulfills the requirements of Directive 98/79/EC on

In vitro diagnostic medical device

Caution (refer to accompanying documents)!

Please consult instructions for use

No special protection against penetrating moisture (IP = International Protection)

ROBERT RIELE GmbH & Co KG Page 2 GENERAL NOTES

Spillings and cleaning

ROBERT RIELE GmbH & Co KG Page 3 GENERAL NOTES

WASTE MANAGEMENT NOTE

Address of the manufacturer:

ROBERT RIELE GmbH & Co KG

Phone: +49 (0)30 404 40 87

QUALITY MANAGEMENT SYSTEM

ROBERT RIELE GmbH & Co KG Page 4 GENERAL NOTES

1 INTRODUCTION TO PHOTOMETER 5010 ................................................ 7

3 OPERATING ELEMENTS ........................................................................... 10

5 CALCULATION PROCEDURES ................................................................. 17

6 METHOD EDITOR ....................................................................................... 44

ROBERT RIELE GmbH & Co KG Page 5 CONTENTS

7 UTILITY PROGRAMS ................................................................................. 47

9 ERROR MESSAGE / CORRECTION .......................................................... 68

10 TECHNICAL DATA ..................................................................................... 71

11 ACCESSORIES AND SPARE PARTS ....................................................... 75

ROBERT RIELE GmbH & Co KG Page 6 CONTENTS

1 INTRODUCTION TO PHOTOMETER 5010

ROBERT RIELE GmbH & Co KG Page 7 1 - INTRODUCTION TO PHOTOMETER 5010

ROBERT RIELE GmbH & Co KG Page 8 2 - INSTALLATION

Indication for the direction of the light in

[DELETE] Window will not be displayed next

(Appears again when overwriting the

[OK] Exit the window. Window appears

M A I N M E N U After around 15 minutes the device is heated

ROBERT RIELE GmbH & Co KG Page 9 2 - INSTALLATION

ROBERT RIELE GmbH & Co KG Page 10 3 - OPERATING ELEMENTS

The surface of the touchscreen may

3.4 WORKING AREA

outlet tube cuvette

joint inlet tube cuvette

ROBERT RIELE GmbH & Co KG Page 11 3 - OPERATING ELEMENTS

3.5 CUVETTES AND CUVETTE ADAPTOR

Switch off the device.

Loosen the milled screw and remove the adaptor.

Direction of the light path:

Direction of the light

3.5.2 Working with original flow-through system

ROBERT RIELE GmbH & Co KG Page 12 3 - OPERATING ELEMENTS

Trigger a normal measuring by [RESULT].

Switch on the pump by the function Pump ON / OFF (chapter 7.2.4.1).

After the installation execute a Pump calibration (chapter 7.2.4.2).

ROBERT RIELE GmbH & Co KG Page 13 3 - OPERATING ELEMENTS

After switch-on the touchscreen shows the main menu.

4.1 Measurement with programmed methods

Call the selected method by [E].

A programmed method can be estab-