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    FG1617 TP5

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    João Domingos
    This document discusses several methodologies used to test for genetic variants including restriction digestion and electrophoresis, Southern and Northern blotting, hybridization, fluorescence in situ hybridization (FISH), various DNA sequencing techniques like chemical sequencing and chain termination sequencing, polymerase chain reaction (PCR) and several PCR-based techniques like PCR-RFLP, PCR-ASO, SSCP, dHPLC, real-time PCR, and microarrays.

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    FG1617 TP5

    Uploaded by

    João Domingos
    13 views3 pages
    This document discusses several methodologies used to test for genetic variants including restriction digestion and electrophoresis, Southern and Northern blotting, hybridization, fluorescence in situ hybridization (FISH), various DNA sequencing techniques like chemical sequencing and chain termination sequencing, polymerase chain reaction (PCR) and several PCR-based techniques like PCR-RFLP, PCR-ASO, SSCP, dHPLC, real-time PCR, and microarrays.

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    FG1617_TP5

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    This document discusses several methodologies used to test for genetic variants including restriction digestion and electrophoresis, Southern and Northern blotting, hybridization, fluorescence in situ hybridization (FISH), various DNA sequencing techniques like chemical sequencing and chain termination sequencing, polymerase chain reaction (PCR) and several PCR-based techniques like PCR-RFLP, PCR-ASO, SSCP, dHPLC, real-time PCR, and microarrays.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    13 views3 pages

    FG1617 TP5

    Uploaded by

    João Domingos
    This document discusses several methodologies used to test for genetic variants including restriction digestion and electrophoresis, Southern and Northern blotting, hybridization, fluorescence in situ hybridization (FISH), various DNA sequencing techniques like chemical sequencing and chain termination sequencing, polymerase chain reaction (PCR) and several PCR-based techniques like PCR-RFLP, PCR-ASO, SSCP, dHPLC, real-time PCR, and microarrays.

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    Download as PDF, TXT or read online from Scribd
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    of 3

    Methodologies

    used to test for gene0c variants

    Restric(on diges(on and electrophoresis. Southern and


    Northern blo7ng. Hybridiza(on, FISH. DNA sequencing
    (chemical, chain termina(on, pyrosequencing).
    Polymerase chain reac(on (PCR) and PCR-based
    techniques (PCR-RFLP, PCR-ASO, SSCP, dHPLC). Real-(me
    PCR. Microarrays.

    PCR RFLP Analysis
    Restriction enzyme analysis

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