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Bio101 Lab Activity 03 - The Microscope
Bio101 Lab Activity 03 - The Microscope
Bio101 Lab Activity 03 - The Microscope
03
THE MICROSCOPE
Introduction
Microscope is a powerful tool that is commonly used in laboratories to observe biological
structures and specimens that are too small to be seen with the unaided eye. It has three major
parts namely: the optical parts, the illuminating parts, and the mechanical parts. For efficient
operation and use of the microscope, one should know its parts and functions. Proper handling,
manipulation, and care of the microscope should always be observed.
Materials
Compound light microscope
Lens paper
Prepared slide of the letter “e”
Transparent plastic ruler
Procedure
A. Parts and Functions of a Compound Light Microscope
1. Borrow a compound light microscope from the biology stockroom.
2. Carefully carry the microscope to your workbench. Place the microscope on the table at
least five (5) inches from the edge. Remove the dust cover.
3. Identify the optical, illuminating, and mechanical parts of the microscope. State the
function(s) of each component part. Complete Figure 3.1 and Table 3.1.
1. Determine the total magnification of the letter “e” under the scanner, LPO, HPO, and
OIO.
2. Record your answers in Table 3.2. Show your computations on the space provided.
For example, if 4.45 mm is the diameter of FOV at 4x magnification (scanner), then the
diameter of the FOV at 10x magnification (LPO) will be:
The circular area of the FOV can be computed using the area of circle as follows:
where: π=3.1416
Using the example above, the area of FOV under a 4x scanning objective with an FOV
diameter equal to 4.45 mm will be:
1. Obtain a transparent plastic ruler with millimeter (mm) metric markings. Place the ruler
on the stage and under the stage clips.
2. Carefully rotate the nosepiece until the scanner is aligned.
3. Focus up and down until the metric markings on the ruler are clear.
4. Align the ruler to measure the diameter of the FOV. The space between each marking on
the ruler should represent a 1-mm interval. Record in Table 3.3 the diameter of FOV
under a scanner magnification.
5. Calculate and record in Table 3.3 the diameter of FOV under an LPO, HPO, and OIO
magnification. Show your computations on the space provided.
6. Calculate and record in Table 3.3 the area of FOV under a scanner, LPO, HPO, and OIO
magnification. Show your computations on the space provided.
Answer Sheet 02
THE MICROSCOPE
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Computation for Total Magnification Computation for FOV Diameter and Area
Scanner
LPO
HPO
OIO
REVIEW QUESTIONS
1. Identify whether the following statements pertaining to the proper care of a microscope are
true or false.
a. Always carry the microscope using both hands.
b. Always carry the microscope in an upright position.
c. Start with the highest power objective when focusing a microscope.
d. Put away the microscope with the lowest power objective in position.
e. Always adjust the knob in a downward direction when focusing the objective.
2. In a properly aligned microscope, objectives are said to be parfocal. What does it mean?
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3. When should the high power objective be used? How about the low-power objective?
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