Cell Cycle Control

G1/S Checkpoint

G1/S Checkpoint

Growth Factor
Receptor Activation
Akt
TGF-β DNA
Replicative Damage
Senescence
FoxO1/3

Differentiation ATM/
ATR

Hormones Smad3
BMI1 Myc
Smad4 p53

Ultra Violet Replicative

Stress Response Senescence

Chk1/2
p21 Cip1
p19 INK4D p18 INK4C p16 INK4A p15 INK4B

Myc Myc
Growth p27 Kip1 Ubiquitination
Factor
Withdrawal
Myc Myc
CDK Inhibitors: cdc25A SCF
GSK-3β
• Palbociclib CUL1 RBX1
• Abemaciclib
Skp1
• Ribociclib
• Trilaciclib

SCF CDK4/6 CDK2

CUL1 RBX1
Cyclin D Cyclin E Ubiquitination
Skp1
R
E S-PH
Ubiquitination -PHAS ASE
G1

Suv39H1
Abl HDAC Rb
Rb E2F/DP Target Genes:
FoxO1 Bim Cyclin E/A, E2F-1/2/3,
E2F E2F
FasL cdc2, c-Myc, p107,
DBE Apoptosis DP-1 DP-1
RanGAP, TK, DHFR,
TRAIL OFF ON
PCNA, H2A, etc.

rev. 03/08/20

For pathway key and background, please visit:

www.cst-science.com/pathway
 Key Products for Cell Cycle Control
Visit www.cellsignal.com for a full listing of antibodies, related kits, and reagents.

ANTIBODIES APPLICATIONS REACTIVITY ANTIBODIES APPLICATIONS REACTIVITY

88439 53BP1 (E7N5D) XP® Rabbit mAb WB, IHC-P, IF-IC H 64016 p21 Waf1/Cip1 Antibody (Mouse Preferred) WB, IP M
2675 Phospho-53BP1 (Ser1778) Antibody WB, IF-IC, F H, Mk 3686 p27 Kip1 (D69C12) XP® Rabbit mAb WB, IP, IF-IC, F H, R, Mk
10519 Ape1 (E5Y2C) Rabbit mAb WB, IHC-P, IF-IC H, M, R 2527 p53 (7F5) Rabbit mAb WB, IHC-P, IF-IC, F, ChIP H, Mk
2873 ATM (D2E2) Rabbit mAb WB H, M 32532 p53 (D2H9O) Rabbit mAb (Rodent Specific) WB, IP, ChIP M, R
13050 Phospho-ATM (Ser1981) (D25E5) Rabbit mAb WB, F H, (Mk) 9286 Phospho-p53 (Ser15) (16G8) Mouse mAb WB, IF-IC, F H
13934 ATR (E1S3S) Rabbit mAb WB, IP H, M, R 9284 Phospho-p53 (Ser15) Antibody WB, IP, ChIP H, M, R, Mk, (Mi, B, Pg)
30632 Phospho-ATR (Thr1989) (D5K8W) Rabbit mAb WB H 13109 p63-α (D2K8X) XP® Rabbit mAb WB, IP, IF-IC, F, ChIP, H
ChIP-seq
14823 BRCA1 (A8X9F) Rabbit mAb WB, IP H
13110 PCNA (D3H8P) XP® Rabbit mAb  WB, IP, IHC-P, IF-F, IF-IC, F H, M, R, Mk
10741 BRCA2 (D9S6V) Rabbit mAb WB H
8875 Rad51 (D4B10) Rabbit mAb WB, IP H, M, R, Mk
2561 Phospho-CDK2 (Thr160) Antibody WB, IP, F, E-P H, M, R
9309 Rb (4H1) Mouse mAb WB, IP, IHC-P, IF-IC, F, ChIP H, Mk, B, Pg
12790 CDK4 (D9G3E) Rabbit mAb WB, IHC-P, IF-IC, F H, Mk
9313 Rb (D20) Rabbit mAb WB, IP, ChIP H, M, Mk
13331 CDK6 (D4S8S) Rabbit mAb WB, IP H, Mk
8180 Phospho-Rb (Ser780) (D59B7) Rabbit mAb WB, IP H, M, R, Mk
11973 CDK12 Antibody WB, IP H, Mk
8516 Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M, R, Mk
8064 CDT1 (D10F11) Rabbit mAb WB, IP, IF-IC H, Mk
35869 RPA32/RPA2 (E8XP5) XP® Rabbit mAb WB, IP, IHC-P, IF-IC H
2360 Chk1 (2G1D5) Mouse mAb WB H, M, R, Mk
54762 Phospho-RPA32/RPA2 (Ser8) (E5A2F) WB, IF-IC, F H
12302 Phospho-Chk1 (Ser317) (D12H3) XP ®
WB, IP, IF-IC H, M, Mk
Rabbit mAb
Rabbit mAb
65939 RRM2 (E7Y9J) XP® Rabbit mAb WB, IHC-P, IF-IC H
6334 Chk2 (D9C6) XP® Rabbit mAb WB, IP, IHC-P, IF-IC H
28755 Thymidine Kinase 1 (E2H7Z) Rabbit mAb WB, IP, IF-IC, F H
2197 Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb WB, IP, IHC-P, F H
13439 Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb WB, IP H
55506 Cyclin D1 (E3P5S) XP Rabbit mAb
®
WB, IP, IHC-P, IF-IC, F H, M, R
20808 Cyclin E1 (D7T3U) Rabbit mAb WB H, M, R KITS & RELATED REAGENTS
67825 DeltaN p63 (E6Q3O) Rabbit mAb WB, IP, IF-IC, ChIP H 7037 PathScan® Phospho-Chk2 (Thr68) Sandwich ELISA Kit
38168 DNA-PKcs (E6U3A) Rabbit mAb WB, IHC-P, IF-IC, F H 7870 PathScan® Phospho-Chk1 (Ser317) Sandwich ELISA Kit
68716 Phospho-DNA-PKcs (Ser2056) (E9J4G) Rabbit mAb WB H 7167 PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Kit
52508 Geminin (E5Q9S) XP Rabbit mAb WB, IP, IHC-P, IF-IC H 13152 PathScan® Phospho-Rb (Ser807/811) Sandwich ELISA Kit
80312 Phospho-Histone H2A.X (Ser139) (D7T2V) WB, IHC-P, IF-IC, F H, M, R, Mk 13016 PathScan® Phospho-Rb (Ser780) Sandwich ELISA Kit
Mouse mAb
9932 Cell Cycle Regulation Antibody Sampler Kit
9027 Ki-67 (D2H10) Rabbit mAb (IHC Specific) IHC-P H
9917 Cell Cycle/Checkpoint Antibody Sampler Kit
86934 MDM2 (D1V2Z) Rabbit mAb WB, IP, IF-IC H
17498 Cell Cycle Phase Determination Antibody Sampler Kit
2017 MSH2 (D24B5) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M
9947 DNA Damage Antibody Sampler Kit
74560 p14 ARF (E3X6D) Rabbit mAb WB, IHC-P, IF-IC, F H
4084 DRAQ5®
80772 p16 INK4A (D7C1M) Rabbit mAb WB, IP, F H
4087 Propidium Iodide (PI)/RNase Staining Solution
2947 p21 Waf1/Cip1 (12D1) Rabbit mAb WB, IP, IHC-P, IF-IC, F H, Mk, (Dg)

Untreated Nutlin 3a-treated

7
La

CF

kDa
He

M
For Research Use Only. Not For Use In Diagnostic Procedures. 19-CEL-1114-PWH-MAY22

200
140
100
80
60
50
40
30

20
Cyclin D1 (E3P5S) XP® Rabbit mAb #55506: MDM2 (D1V2Z) Rabbit mAb #86934: Confocal IF analysis of U-2 OS cells, untreated p16 INK4A Phospho-Rb (Ser807/811) (D20B12) XP®
IHC analysis of paraffin-embedded human lung (left) or treated with Nutlin 3a (10 μM, 24 hr; right), using #86934 (green). Actin filaments 60
Rabbit mAb #8516: IIHC analysis of paraffin-
adenocarcinoma using #55506. were labeled with DyLight™ 554 Phalloidin #13054 (red). 50 embedded human colon carcinoma using #8516.
40 β-Actin

p16 INK4A (D7C1M) Rabbit mAb

#80772: WB analysis of extracts from
HeLa and MCF7 cells using #80772
(upper) or β-Actin (D6A8) Rabbit mAb
#8457 (lower).

© 2022 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, and XP are trademarks of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

For pathway key and background, please visit:

www.cst-science.com/pathway