3/9/2022

1 Bacterial Growth : 2

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Nutritional Requirements
NRS 1205: ❑ Carbon

Microbiology ✓ Autotrophs- utilize inorganic compounds and

inorganic salts as Carbon source

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✓ Organotrophs- use organic substances (glucose) as
their carbon source
✓ Photolithotrophs
✓ Photoorganotrophs
✓ Chemolititrophs
✓ Chemoorganotrophs

Bacterial Growth : 3 Bacterial Growth : 4

Nutritional Requirements Nutritional Requirements

❑ Nitrogen, Sulfur, Phosphorus ❑ Inorganic Ions
✓ N and P –nucleic acids and ATP ✓ Magnesium
✓ N and S - proteins ✓ Potassium
✓ Calcium
✓ Iron
✓ Trace elements (Manganese, Zinc, Copper cobalt)

Bacterial Growth : 5 Bacterial Growth : Physical 6

Nutritional Requirements Requirements

❑ Growth Factors ❑ Water
✓ Vitamin B Complex ❑ Oxygen
✓ Amino Acids ✓ Aerobes
✓ Obligate Anaerobes
✓ Facultative Organisms
❖ Microaerophiles
❖ Capnophiles

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Bacterial Growth : Physical 7 Bacterial Growth : Physical 8

Requirements Requirements
❑ Temperature ❑ pH
✓ Thermophiles- higher than 40 C ✓ Alkalophiles- ph 8.4- 9
✓ Mesophiles- 20-40 C ✓ Neutrophiles- 6.5-7.5
✓ Psychrophiles-10-20 C ✓ Acidophiles-less than 6

Bacterial Growth : Physical 9 Bacterial Growth Curve 10

Requirements
❑ Osmotic Conditions ❑ Lag Phase
✓ Halophiles- high salt concentration ✓ No applicable increase in number of organism
✓ Osmophiles- high osmotic pressure ✓ 1-4 hours

Bacterial Growth Curve 11 Bacterial Growth Curve 12

❑ Log Phase ❑ Stationary Phase

✓ Logarithmic/ Exponential phase ✓ Period of equilibrium
✓ Rapid Cell division ✓ Rate of growth slows
✓ 8 hours ✓ Nutrient starts to deplete
✓ Toxic wastes accumulate

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Bacterial Growth Curve 13 The End 14

❑ Death
✓ Decline Phase
✓ Varies from few hours to days