CA-1500 SM #2 Hydraulic&Mech

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SECTION 2 HYDRAULIC/MECHANICAL SYSTEMS
2.1HYDRAULIC DIAGRAM ---------------------------------------------------------------------------------------- 1
2.2 OPERATION OF THE HYDRAULIC SYSTEM ------------------------------------------------------- 2

2.2.1 Aspiration/Dispense of a Sample/Reagent ---------------------------------------------------- 2
2.2.2 Rinsing Pipette --------------------------------------------------------------------------------------- 2
2.2.3 Operation Procedures After Starting Analysis ------------------------------------------------ 3
2.2.4 Analysis Operation ---------------------------------------------------------------------------------- 9
2.2.5 Shutdown Operation ------------------------------------------------------------------------------ 12
2.2.6 Rinsing Pipette(During Analysis)--------------------------------------------------------------- 13
2.3DETECTING UNIT--------------------------------------------------------------------------------------------- 15
2.3.1 Outline------------------------------------------------------------------------------------------------ 15
2.3.2 Specifications/Functions ------------------------------------------------------------------------- 15
2.3.3 Assembly Drawing--------------------------------------------------------------------------------- 16
2.4COOLER UNIT NO. 8 ----------------------------------------------------------------------------------------- 17
2.4.1 Outline------------------------------------------------------------------------------------------------ 17
2.5SAMPLE PLATE HOLDER ---------------------------------------------------------------------------------- 19
2.5.1 Outline------------------------------------------------------------------------------------------------ 19
2.6ROTARY FEEDER NO. 2 ------------------------------------------------------------------------------------ 20
2.6.1 Outline------------------------------------------------------------------------------------------------ 20
2.7SAMPLE FEEDER --------------------------------------------------------------------------------------------- 21
2.7.1 Outline------------------------------------------------------------------------------------------------ 21
2.8TRASH UNIT ---------------------------------------------------------------------------------------------------- 22
2.8.2 Assembly Diagram -------------------------------------------------------------------------------- 22
2.9SAMPLER (OPSU-6)------------------------------------------------------------------------------------------ 23
2.9.1 Outline------------------------------------------------------------------------------------------------ 23
CA-1500 S/M December 2000 Revised

SECTION 2 HYDRAULIC/MECHANICAL SYSTEMS
2.1 HYDRAULIC DIAGRAM

The hydraulic of the CA-1500 whole system as below:
2
Reagent syringe
Reagent pipette
MV4 (with heating 2
M
function)
Rinse cup
MV5
MV6
Sample syringe Sample aspiration

pipette
MV1
M
Rinse cup
MV2
MV3
Cooler
r c unit No. 8
MV11
Vacuum (Source
Distilled water pressure)
MV8
Vacuum (Source pressure) 1.0 kg/cm2
2
1.0 kg/cm MV7
MV10
Distilled water
(Rinse) Waste
CA-1500 SM 2-1 December 2000 Revised

2.2 OPERATION OF THE HYDRAULIC SYSTEM
2.2.1 Aspiration/Dispense of a Sample/Reagent

(1) Aspiration/Dispense of a Sample
1) Sample aspiration
a) The sample pipette descends into the test tube. After the sample fluid surface is
detected, the sample syringe descends to aspirate the sample of the volume
required for one test.
b) The sample pipette descends into the sample plate of the Sample Plate Holder.
After the sample fluid surface is detected, the sample syringe descends to
aspirate the sample of the volume required for one test.
2) Sample dispense
a) After the sample pipette descends into the sample plate of the Sample Plate
Holder, the sample syringe ascend to dispense the sample of the volume required
for one test.
b) After the sample pipette descends into the sample tube of the CA-1500 feeder,
the sample syringe ascends to dispense the sample of the volume required for
one test.
(2) Aspiration/Dispense of Reagent
1) Reagent aspiration
a) The reagent pipette descends into the reagent bottle of the No. 8 low-temperature
unit. After the reagent fluid surface is detected, the reagent syringe descends to
aspirate the reagent of the volume required for one test.
2) Reagent dispense
a) After the reagent pipette descends into a sample tube that has been carried by the
catcher mechanism, the reagent syringe ascend to dispense the reagent for the
volume required for one test.
2.2.2 Rinsing Pipette

(1) Rinsing Sample Pipette
1) The sample pipette descends into the rinse cup in the No. 1 diluent holder assembly.
2) Solenoid valves 1 and 2 are simultaneously turned ON, and the pipette inner surface
is rinsed for 1.5 seconds.
3) After 1.5 seconds, solenoid valve 3 is turned ON, for one second, and the outer
surface of the pipette is rinsed.
(2) Rinsing Reagent Pipette
1) After dispensing a reagent, the reagent pipette descends into the rinse cup of the No.
8 low-temperature unit.
2) Solenoid valves 4 and 5 are simultaneously turned ON, and the pipette inner surface
is rinsed.
3) After 0.5 seconds, solenoid valve 6 is turned ON for 0.4 second, and the outer
4) After 1.8 seconds, the reagent pipette moves to the detergent holder of the No. 8 low-
temperature unit.
5) After 2 seconds, the reagent pipette descends into the detergent holder.
6) After 3.2 seconds, detergent aspiration is performed (for at least one second).

7) After 4.9 seconds, the reagent pipette moves to the rinse cup of the No. 8 low-
temperature unit. After 5.4 seconds, the reagent pipette descends into the rinse cup.
8) After 6.2 seconds, solenoid valves 4 and 5 are simultaneously turned ON, and the
inner surface of the pipette is rinsed for 3 seconds.
9) After 9.2 seconds, solenoid valve 6 is turned ON for one second, and the outer
2.2.3 Operation Procedures After Starting Analysis
(1) The instrument does not become READY until the temperature comes to be in the
specified range. (It becomes READY in the Service Mode.)
(2) Check Sample Plate, remained reagent volume (only when set), and reaction tube trash
box.
(3) Monitor the float SW of external detergent container and waste container. (only when set)
(4) Check Light Shield Cover and STAT Cover switches.
(5) Starting Pneumatic Unit:

1) Release the remained pressure:
Pneumatic Unit does not start if the pressure is remained. Therefore, the remained
2
pressure exceeds 0.3 kg/cm , start the pressure releasing sequence. In this
sequence, the solenoid valve ON/OFF repeats for approximately 8 seconds. After
the sequence is completed, start the pneumatic unit.
2) Drain water in Cooler Unit:
After starting the Pneumatic Unit, the solenoid valve (MV-18) turns ON for a few
seconds to drain water in Cooler Unit.
3) Drain Waste Chamber:
The solenoid valve Chamber turns ON for 2 seconds to drain waste from Waste.
4) Start monitoring pressure error.
(6) Starting Sampler Unit:

1) Initialize Sampler Mechanism:
2) Transfer Racks:
When there is any rack on Sampler Unit, it is fed in and shifted to the aspiration
position .

(7) Initializing Mechanism Units (Lamp Unit, Sample Arm, Reagent Arm and Catcher):
1) Initialize Lamp Unit:
(a) Reset Optical Filter Unit to the home position.
NOTE: If it cannot be reset, “Lamp Unit Move Error” occurs, and the operation
immediately stops.
• Error Detection Condition
1) Although the motor is activated, Optical Filter Unit cannot move
from the home position sensor.
2) The motor does not stop at the home position sensor.
• Possible Cause
1) Failed motor
2) Optical Filter Unit cannot move physically.
3) Home position sensor malfunctions.
4) Failed motor drive circuit
2) Initialize Catcher Unit:

(a) Move Sample Pipette, Reagent Pipette to the upper limit point (home position
sensor).
NOTE: If they cannot be moved, “Sample Arm Move Error (Z)” or “Reagent Arm
Move Error (Z)” occurs, and the operation immediately stops.
• Possible Cause
1) Failed motor
2) Failed home position sensor
(b) Catcher Unit Z ascends to the move position (which is 28 mm above the current
position).
(c) Reset Catcher Unit XY to the home position.
NOTE: If it cannot be reset, “Catcher Move Error (X) or (Y)” occurs, and the
operation immediately stops.
1) Although the motor is activated, Catcher Unit XY cannot move from
the home position sensor.
• Possible Cause
1) Failed motor
2) Catcher Unit XY cannot move physically.

(d) Reset Catcher Unit Z to the home position.
NOTE: If it cannot be reset, “Catcher Move Error (Z)” occurs, and the operation
immediately stops.
1) Although the motor is activated, Catcher Unit Z cannot move from
• Possible Cause
1) Failed motor
2) Catcher Unit Z cannot move physically.
(e) Catcher Unit moves to Trash Unit and discards reaction tubes. Usually, there are
no tubes on Catcher Unit, however, when the operation stops due to an error,
there may be any reaction tubes on Catcher Unit.
NOTE: If Catcher Unit cannot correctly discard the reaction tubes, “Catcher
Move Error (Trash Unit)” occurs and the operation immediately stops.
1) Catcher Unit Z cannot move from the home position sensor 0.7
seconds after starting descending.
• Possible Cause
1) Catcher Unit Z cannot descend because Trash Unit is full of
reaction tubes.
2) Catcher Unit Z cannot descend due to the incorrect XY position.
(f) Catcher Unit XY moves to the home position and Catcher Unit Z ascends to the
move position.
3) Initialize Sample Arm:

(a) Reset Sample Arm Z to the home position.
NOTE: If it cannot be reset, “Sample Arm Move Error (Z)” occurs, and the
1) Although the motor is activated, Sample Arm Z cannot move from
• Possible Cause
1) Failed motor
2) Failed pipette crash sensor (The sensor does not turn OFF.)
4) Sample Arm Z cannot move physically.

(b) Reset Sample Arm XY to the home position.
NOTE: If it cannot be reset, “Sample Arm Move Error (X) or (Y)” occurs, and the
1 Although the motor is activated, Sample Arm XY cannot move from
• Possible Cause
1) Failed motor
4) Sample Arm XY cannot move physically.
4) Initialize Reagent Arm:

(a) Reset Reagent Arm Z to the home position.
NOTE: If it cannot be reset, “Reagent Arm Move Error (Z)” occurs, and the
1) Although the motor is activated, Reagent Arm Z cannot move from
• Possible Cause
1) Failed motor
2) Failed pipette crash sensor (The sensor does not turn OFF.)
4) Reagent Arm Z cannot move physically.
(b) Reset Reagent Arm XY to the home position.
NOTE: If it cannot be reset, “Reagent Arm Move Error (X) or (Y)” occurs, and
the operation immediately stops.
1) Although the motor is activated, Reagent Arm XY cannot move
from the home position sensor.
• Possible Cause
1) Failed motor
4) Reagent Arm XY cannot move physically.

(8) Activating Reaction Tube Feeder Unit:
1) Activating Hopper:
Drive the hopper motor until Reaction Tube Detection Sensor installed on the rail of
Reaction Tube Feeder Unit turns ON.
After the hopper motor is driven for 60 seconds, when Reaction Tube Detection
Sensor does not turn ON, the message “Replenish Reaction Tube” is displayed.
After the hopper motor is driven for 120 seconds, when Reaction Tube Detection
Sensor does not turn ON, the message “No Reaction Tubes” is displayed and the
analysis operation does not start.
2) Activating Rotation Feeder Unit:

Turn the Rotation Feeder Unit motor ON 3 seconds after the Reaction Tube
Detection Sensor installed on the rail turns ON. When Reaction Tube Detection
Sensor attached to Rotation Feeder Unit turns ON, the motor turns OFF.
10 seconds after the Rotation Feeder Unit motor ON, if Reaction Tube Detection
Sensor attached to Rotation Feeder Unit turns OFF, “Reaction Tube Clogging” error
occurs and the analysis operation does not start.
(9) Supplying and draining water:

1) Supplying water to Detergent Chamber:
NOTE: If the supplying operation is not correctly completed, the message “No
Detergent” is displayed and the analysis operation does not start.
1) After 30 seconds from starting supplying water (Solenoid Valve No. 9
turns ON), Detergent Chamber cannot be filled with detergent and
the float switch of Detergent Chamber is OFF (open).
• Possible Cause
1) Pneumatic Unit malfunctions or incorrect pressure
2) Clogging in the supplying line
3) Failed float switch
2) Draining water from Waste Chamber:
NOTE: If the draining operation is not correctly completed, the message “Drain
Waste Error” is displayed and the analysis operation does not start.
1) After 2 seconds from starting draining water (Solenoid Valve No. 7
turns ON), Waste Chamber cannot be drained and the float switch of
Waste Chamber is OFF (open).
• Possible Cause
1) Pneumatic Unit malfunctions or incorrect pressure
2) Clogging in the draining line
3) Failed float switch

(10) Rinsing Sample Pipette, Reagent Pipette:
1) Descending Sample Arm Z (Pipette), Reagent Arm Z (Pipette):
Each pipette descends into Rinse Cup.
NOTE: If it cannot be correctly descended, “Sample or Reagent Arm Pipette

Crash” occurs and the operation immediately stops.
1) After the motor stops, Pipette Crash Sensor turns ON.
• Possible Cause
1) Pipette position is incorrect.
2) Reset Sample Syringe Mechanism and Reagent Syringe Mechanism:

Reset each Syringe Mechanism.
NOTE: If it cannot be correctly reset, “Sample or Reagent Syringe Mechanism

Move Error” occurs and the operation immediately stops.
1) Although the motor is activated, each Syringe Mechanism cannot
move from the home position sensor.
• Possible Cause
1) Failed motor
4) Sample syringe Mechanism or Reagent Syringe Mechanism cannot
move physically.
3) Rinsing Sample Pipette, Reagent Pipette

No error monitoring

2.2.4 Analysis Operation
For example, the analysis operation when three parameters, PT, APTT and Fbg of a sample are
analyzed in the standard mode, the operation goes as described below.
(1) Rinsing with using CA-CLEAN I of Reagent Pipette (Reagent Arm Mechanism):
Rinse Reagent Pipette three times with using CA-CLEAN I in order to maintain the
Reagent Pipette’s stability. 100µL of CA-CLEAN I aspirated once, therefore, total of 300
µL of CA-CLEAN I is required.
If there is no CA-CLEAN I, “Replenish Reagent” error occurs and the operation is
interrupted.
This sequence is operated with lapped with the Sample Arm sequence.
(2) Dispensing from Sampler (Sample Arm Mechanism):

1) Sample Pipette moves from Rinse Cup to Sampler and descend to the surface of the
liquid in a test tube, and then aspirate the required volume of sample (PT 50µL +
APTT 50 µL + Fbg 10 µL + dead volume of sample plate 50 µL --- total 160 µL). At
this time, if Liquid Surface Sensor cannot detect the surface, “No Sample” error
occurs.
After aspirating sample, Sample Pipette ascends. At this time, by monitoring Liquid
Surface Sensor, any change cannot be detected, “Sample Arm Sampling Error”
occurs.
2) Sample Pipette moves to Sample Plate and descends. When the descending is
completed, by checking Liquid Surface Sensor, if the surface is not detected,
“Sample Plate Error” occurs and the operation is interrupted.
Also, by monitoring Pipette Crash SW, if the Pipette Crash SW is ON, “Sample Arm
Pipette Crash” occurs and the operation immediately stops.
3) Drain sample. After draining, Sample Pipette ascends.
4) Sample Pipette moves to Rinse Cup and descends.
When the descending is completed, by monitoring Pipette Crash SW, if the Pipette
Crash SW is ON, “Sample Arm Pipette Crash” error occurs and the operation
immediately stops.
5) Rinse Sample Pipette.

(3) Dispensing from Sample Plate, moving to Heater Unit (Sample Arm Mechanism, Catcher
Mechanism):
1) Dispensing PT Sample:
(a) Sample Pipette moves from Rinse Cup to Sample Plate, descends to the liquid
surface, and aspirate the required volume of the sample (50 µL). At this time, if
Liquid Surface Sensor cannot detect the surface, “Short Sample” error occurs.
After aspirating sample, Sample Pipette ascends. At this time, by monitoring
Liquid Surface Sensor, if the change of the surface cannot be detected, “Sample
Arm Sampling Error” occurs.
(b) Sample Pipette moves to Rotation Feeder.
(c) Check if there are any reaction tubes on Rotation Feeder Unit. If Reaction Tube
Detection Sensor on Rotation Feeder indicate that there is no reaction tube,
“Reaction Tube Clogged” error occurs and the operation is interrupted.
(d) Sample Pipette descends. When the descending is completed, by monitoring
Pipette Crash SW, if the Pipette Crash SW is ON, “Sample Arm Pipette Crash”
error occurs and the operation immediately stops.
(e) Drain sample. After draining, Sample Pipette ascends.
(f) Sample Pipette moves to Rinse Cup and descends.
When the descending is completed, by monitoring Pipette Crash SW, if the
Pipette Crash SW is ON, “Sample Arm Pipette Crash” occurs and the operation
immediately stops.
(g) Rinse Sample Pipette.
(h) Catcher XY moves to Rotation Feeder Unit and Catcher Z descends.
(i) Catcher Z catches the sample tube and ascends.
(j) After Catcher Z ascends, if Reaction Tube Detection Sensor of Rotation Feeder
Unit does not turn OFF in 2 seconds, “Reaction Tube Catching Error” occurs and
the operation is interrupted.
(k) Catcher XY moves to Heater Unit and Catcher Z descends. When Catcher Z
cannot correctly descend, “Catcher Move Error (Lower)” occurs and the operation
immediately stops. (This error occurs if a reaction tube is placed twice or located
at the incorrect position.)
(l) Catcher Z ascends to the move position.
(m) Catcher XY is reset to the home position.
2) Dispensing APPT Sample:

Same as PT

3) Dispensing Fbg Sample:
(a) Sample Pipette moves from Rinse Cup to Diluent Holder position, descends to the
liquid surface, and aspirate the required volume of diluent (90 µL). At this time, if
Liquid Surface Sensor cannot detect the surface, “No Diluent” error occurs and
the operation is interrupted.
The descending pulse is decided depending on the type of containers set on
Reagent Information Settings display screen. And, if the container is different
from the set container, the dead volume of the diluent increases or “No Diluent”
error does not occur even if no diluent is left.
After aspirating diluent, Sample Pipette ascends. At this time, by monitoring
Liquid Surface Sensor, if the change of the surface cannot be detected, “Diluent
Sampling Error” occurs.
(b) Sample Pipette moves to Sample Plate, descends to the liquid surface, and
aspirates the required volume of sample (10 µL).
Same as PT as for the procedure from now on.
(4) Dispensing Reagent (Catcher Mechanism, Reagent Arm Mechanism):

At the preset time for dispensing reagent (incubation time), each reagent is dispensed.
1) Dispensing APTT Reagent:

(a) Reagent Pipette moves from Rinse Cup to Reagent Holder position, descends to
the liquid surface, and aspirates the required volume of the reagent (50 µL). At
this time, if Liquid Surface Sensor cannot detect the liquid surface, “No Reagent”
error occurs and the operation is interrupted.
The descending pulse is decided depending on the types of the containers set on
Reagent Information Settings display screen, therefore, if the container is different
from the preset container type, the dead volume of the reagent increases, or even
if there is no reagent left, “No Reagent” error does not occur.
After aspirating the reagent, Reagent Pipette ascends. At this time, by monitoring
Liquid Surface Sensor, if the change of the surface cannot be detected, “Reagent
Sampling Error” occurs.
(b) Catcher XY moves to Heater Unit, and Catcher Z descends and catches a
reaction tube. Catcher Z ascends to the move position.
(c) Reagent Pipette moves to the reagent dispensing position (upper part of Rinse
Cup) after aspirating reagent.
(d) Catcher XY moves to the reagent dispensing position.
(e) Reagent Pipette drains the reagent, and Catcher Z mixes the sample.
(f) Catcher XY moves to Heater Unit (Detector Unit), and Catcher Z descends. If the
Catcher Z cannot correctly descend, “Catcher Move Error (Lower)” occurs and the
operation immediately stops. (This error occurs if a reaction tube is placed twice
or located at the incorrect position.)
(g) Reagent Pipette descends to Rinse Cup.
When the descending is completed, by monitoring Pipette Crash SW, if the
Pipette Crash SW is ON, “Reagent Arm Pipette Crash” occurs and the operation
immediately stops.
(h) Catcher Z ascends to the move position.
(i) Catcher XY is reset to the home position.

2) Dispensing PT Reagent:
The procedure is almost same as the one for APTT reagent dispensing. However,
the required volume for aspiration of reagent is 100 µL. And also, after draining
reagent, Catcher XY moves to Detector Unit and starts calculating the clotting time
for the sample.
3) Dispensing Calcium Chloride (APTT):
The procedure is almost same as the one for APTT reagent dispensing. However,
after draining reagent, Catcher XY moves to Detector Unit and starts calculating the
clotting time for the sample.
(4) Dispensing Fbg Reagent:

The procedure is almost same as the one for APTT reagent dispensing. However, after
draining reagent, Catcher XY moves to Detector Unit and starts calculating the clotting
time for the sample. And also, after draining reagent, CA-CLEAN rinsing is performed for
Reagent Pipette.
(5) Discarding Reaction Tube after analysis:

After calculating each clotting time for test parameter, Catcher transfers the reaction tube
in Detector Unit to Trash Unit.
1) Catcher XY moves to Detector Unit, and Catcher Z descends and catches the
reaction tube.
2) Catcher Z ascends to the move position.
3) Catcher XY moves to Trash Unit and Catcher Z descends. If Catcher Z cannot
correctly descend, “Catcher Move Error (Trash Unit)” occurs and the operation
immediately stops. (This error occurs when Trash Unit is full of reaction tubes or the
Catcher cannot transfer the reaction tube to the correct position.)
2.2.5 Shutdown Operation

When all the reaction tubes in Detector Unit are discarded, and there is no new analysis order,
the shutdown operation is performed. When the shutdown operation is completed, the
instrument becomes READY.
(1) Move Sample Pipette to the upper part of Rotation Feeder Unit.
(2) Move Reagent Pipette to the upper part of Rinse Cup.
(3) Turn Pneumatic Unit OFF.

2.2.6 Rinsing Pipette(During Analysis)
(1) Rinsing Sample Pipette
1) Sample Pipette descends to Rinse Cup.
2) Turn SV2 ON for 2.5 seconds. The waste in Rinse Cup is drained.
3) Turn SV1 ON for 1.5 seconds. Rinsing inside Sample Pipette is performed.
4) After 1.5 seconds, turn SV3 ON for 1.0 second. Rinsing outside Sample Pipette is
performed.
Required detergent volume for rinsing inner Sample Pipette: approx. 1700 µL
Required detergent volume for rinsing outer Sample Pipette: approx. 2200 µL
In the FDP, D-Dimmer Analysis Modes, the rinsing sequence using CA CLEAN I is
performed after aspirating sample. This sequence is operated for 30 seconds. After
aspirating CA CLEAN I, the above Sample Pipette rinsing is performed twice. The
required aspiration volume of CA CLEAN I is: Sample Aspiration Volume + Diluent
Volume + a
This sequence can be set to ON or OFF in the Analysis Procedure Settings, also the
detergent can be changed.
(2) Rinsing Reagent Pipette

1) Rinsing Reagent Pipette before aspirating sample
This rinsing sequence is operated when the detergent is set to be used (before) in the
Analysis Procedure Settings.
(a) Reagent Pipette moves to Detergent Holder.
(b) Reagent Pipette aspirates detergent. The required detergent volume is the same
as the reagent aspiration volume.
The required detergent aspiration volume on the default settings is described in the
following table.
(µL)
CA CLEAN I CA CLEAN II
AT III R1 100 –
AT III R2 100 –
a 2PI R1 100 –
a 2PI R2 – –
Plg R1 100 –
Plg R2 – –
PC R1 100 –
PC R2 – –
(c) Reagent Pipette moves to Rinse Cup.

(d) Turn SV5 ON for 4.0 seconds. The waste in Rinse Cup is drained.
(e) Turn SV4 ON for 3.0 seconds. Inside Reagent Pipette is rinsed.
(f) After 3.0 seconds, turn SV6 On for 1 second. Outside Reagent Pipette is rinsed.
Required detergent volume for rinsing inner Reagent Pipette: approx. 1200 µL
Required detergent volume for rinsing outer Reagent Pipette: approx. 2200 µL

2) Rinsing Reagent Pipette after dispensing reagent
This rinsing sequence is operated when the detergent is set to be used (after) in the
Analysis Procedure Settings.
(a) Reagent Pipette descends to Rinse Cup.
(b) Turn SV5 ON for 0.9 seconds. The waste in Rinse Cup is drained.
(c) Turn SV4 ON for 0.9 seconds. Inside Reagent Pipette is rinsed.
(e) After 0.5 seconds, turn SV6 ON for 0.4 seconds. Outside Reagent Pipette is
rinsed.
(g) Reagent Pipette moves to Detergent Holder.

(h) Reagent Pipette aspirates detergent. The aspirated volume is the same as the
reagent aspiration.
The required detergent aspiration volume on the default settings is described in
the following table.
(µL)
CA CLEAN I CA CLEAN II
Fbg 50
TT 50
AT III R1 100 –
AT III R2 – 100
a 2PI R1 100
a 2PI R2 100 –
Plg R1 100 –
Plg R2 – –
PC R1 100 –
PC R2 100 –
3) Rinsing Reagent Pipette after dispensing reagent or after aspirating detergent

(a) Reagent Pipette descends to Rinse Cup.
(b) Turn SV5 ON for 4.0 seconds. The waste in Rinse Cup is drained.
(c) Turn SV4 ON for 3.0 seconds. Inside Reagent Pipette is rinsed.
(c) After 3.0 seconds, turn SV6 ON for 1 second. Outside Reagent Pipette is rinsed.

2.3 DETECTING UNIT
2.3.1 Outline
The CA-1500 detecting unit provides four detecting blocks, each of which provides four
detecting wells and seven incubator wells.
The temperature of each detecting unit is maintained at 37°C, with the heater and
thermistor.
For coagulation detection, light emitted from the red LED is directed to a sample. The light
is scattered by 90° and is received by the photo-diode, then A/D conversion is performed.
For transmitted-light detection, light emitted from the halogen lamp is directed to a sample
in the detecting port. The transmitted light is received by the photo-diode, then A/D
conversion is performed.
2.3.2 Specifications/Functions
(1) Coagulation Detection Unit
Photometer : Four wells
Incubator : Seven wells
Light source : 660 nm LED
Heater : 30 W (Film heater)
Protecting function : 76°C thermal fuse
(2) Transmitted-light Detection Unit
Photometer : Four wells
Light source : Halogen lamp
Heater : 100 V, 30 W (Film heater)
Protecting function : 76°C thermal fuse

2.3.3 Assembly Drawing
Coagulation Detection Unit
Transmitted-light Detection Unit

2.4 COOLER UNIT NO. 8
2.4.1 Outline
(1) This unit cools coagulation reagents, chromogenic reagents, factor deficit plasma, and
control plasma to prevent these materials from being deactivated.
(2) Precipitable coagulative reagents are stirred.
(3) The pressure air supplied from the pneumatic unit is cooled to saturate the air with
moisture. (Drain function)
NOTE: The moisture of the saturated air is removed by the No. 2 drain filer assembly.
(1) Cooling function
Cooling element: Three Peltier elements
Target temperature : 15 ± 2°C (when room temperature is 15°C to 30°C)
Time required to reach target temperature
: Within 30 minutes (In the range of the specified operating
temperatures)
Radiator : One set of radiator fin and DC fan
(2) Stirring function
Drive : AC synchronous motor
Stirring capacity : The stirrer rotates at approx. 50 rpm.
Stirring position : Four positions at the back of the 31 mm diameter reagent
bottle holder


2.5 SAMPLE PLATE HOLDER
2.5.1 Outline
The Sampler Plate Holdter holds sample plates for primary sampling sample. The of the
sample plate is detected and indicated by the 2-color LEDs. These LEDs also indicate if the
sample plate can be dismounted or not.
Number of mountable plates: Five plates max.
Plate detection: Photo-interrupter TLP1204 (C3)
Indicator: 2-color LEDs
[Reference]
TLP1204(C1) 228-4611-2
310497102
LED : Light emitter No. 18
973-4981-9
* Five sets of the No. 14 light emitters are linked with each other.

2.6 ROTARY FEEDER NO. 2
2.6.1 Outline
The No. 2 rotary feeder is a part of the automatic sample tube feeding function. After
sample tubes are aligned in the feeder, they are picked up one by one on the rotary feeder,
and fed into the catcher. At the feed position, the micro-switch detects the presence or
absence of a sample tube. At the same position, the sample is dispensed.
Feeding method : Fed by the swiveling rotor
Drive : DC motor with gear head (5 V)
Rotation speed : 9 rpm
Feeding capacity : Approx. 2.1 seconds/tube

2.7 SAMPLE FEEDER
2.7.1 Outline
The sampler feeder automatically feeds sample tubes with the rotary feeder. The sample
tubes irregularly placed in the hopper are aligned in the rail of the feeder.
The reflective sensor at the top of the rail detects the presence of a sample tube so that the
sample tube feeder can be activated only when required.
Aligning method : Sample tubes are picked up through vertical motion of the rail
between two arc plates.
Number of sample tubes that can be loaded
: 300 tubes max.
Feeding capacity : Approx. 5 seconds/tube (average)
Drive : DC motor with gear head (12 VDC)
Rotation speed : 120 rpm
Reflective Sensor

2.8 TRASH UNIT
Box detector : One reflective photo-interrupter
Capacity of discharge box : Approx. 280 tubes
Box fixing method : Leaf spring
2.8.2 Assembly Diagram

2.9 SAMPLER (OPSU-6)
2.9.1 Outline
The CA-1500’s sampler (OPSU-6) has been designed based on the CA-6000’s sampler
(OPSU-4). For details, refer to the “CA-6000 Service Manual.”
[Modifications from the OPSU-4]
(1) Removal of the driver board
(2) Removal of the limit sensor for detecting the presence/absence of the sampler center
cover.
(3) Removal of the sensor for detecting the presence/absence of blood.
(4) Addition of a new sensor for detecting the presence/absence of a blood collection tube
(The GP2A22 reflective photo-interrupter is newly employed).
(5) Addition of a new reinforcing plate for fixing the main unit (Warpage caused by the load
applied from the upward can be prevented).
(6) Removal of the ground cable (The reinforcing plate described in above 5) functions to
ground the unit).
Location of each sensor
S NS 1 0 S NS 5 S NS 4 S NS 1 S NS 2
S NS 8
S NS 9
S NS 1 1
S NS 6
S NS 3
Micro -switch se n so r
Th ro u g h b e am se n so r
Re fle ctive se n so r
P h o to -in te rru p te r se n so r
No. Sensor No. Sensor

SNS1 Rack Detection Sensor SNS6 Tube Detection Sensor
SNS2 Rack Feed Sensor SNS8 Shift Home Position Sensor
SNS3 Rack Forward Levers Sensor SNS9 Shift Move Position Sensor
SNS4 Tube Position Sensor (Right) SNS10 Feed Out Home Position Sensor
SNS5 Tube Position Sensor (Left) SNS11 Rack Full Sensor

CA-1500 SM #2 Hydraulic&amp;Mech

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SECTION 2 HYDRAULIC/MECHANICAL SYSTEMS

2.1HYDRAULIC DIAGRAM ---------------------------------------------------------------------------------------- 1

2.2 OPERATION OF THE HYDRAULIC SYSTEM ------------------------------------------------------- 2

CA-1500 S/M December 2000 Revised

2.1 HYDRAULIC DIAGRAM

Sample syringe Sample aspiration

CA-1500 SM 2-1 December 2000 Revised

2.2.1 Aspiration/Dispense of a Sample/Reagent

2.2.2 Rinsing Pipette

CA-1500 SM 2-2 December 2000 Revised

2.2.3 Operation Procedures After Starting Analysis

(5) Starting Pneumatic Unit:

(6) Starting Sampler Unit:

CA-1500 SM 2-3 December 2000 Revised

2) Initialize Catcher Unit:

CA-1500 SM 2-4 December 2000 Revised

3) Initialize Sample Arm:

CA-1500 SM 2-5 December 2000 Revised

4) Initialize Reagent Arm:

(b) Reset Reagent Arm XY to the home position.

CA-1500 SM 2-6 December 2000 Revised

2) Activating Rotation Feeder Unit:

(9) Supplying and draining water:

2) Draining water from Waste Chamber:

CA-1500 SM 2-7 December 2000 Revised

NOTE: If it cannot be correctly descended, “Sample or Reagent Arm Pipette

2) Reset Sample Syringe Mechanism and Reagent Syringe Mechanism:

NOTE: If it cannot be correctly reset, “Sample or Reagent Syringe Mechanism

3) Rinsing Sample Pipette, Reagent Pipette

CA-1500 SM 2-8 December 2000 Revised

(2) Dispensing from Sampler (Sample Arm Mechanism):

CA-1500 SM 2-9 December 2000 Revised

2) Dispensing APPT Sample:

CA-1500 SM 2-10 December 2000 Revised

(4) Dispensing Reagent (Catcher Mechanism, Reagent Arm Mechanism):

1) Dispensing APTT Reagent:

CA-1500 SM 2-11 December 2000 Revised

(4) Dispensing Fbg Reagent:

(5) Discarding Reaction Tube after analysis:

2.2.5 Shutdown Operation

CA-1500 SM 2-12 December 2000 Revised

(2) Rinsing Reagent Pipette

(c) Reagent Pipette moves to Rinse Cup.

CA-1500 SM 2-13 December 2000 Revised

(g) Reagent Pipette moves to Detergent Holder.

3) Rinsing Reagent Pipette after dispensing reagent or after aspirating detergent

CA-1500 SM 2-14 December 2000 Revised

CA-1500 SM 2-15 December 2000 Revised

Coagulation Detection Unit

Transmitted-light Detection Unit

CA-1500 SM 2-16 December 2000 Revised

CA-1500 SM 2-17 December 2000 Revised

CA-1500 SM 2-18 December 2000 Revised

2.5.3 Assembly Drawing

CA-1500 SM 2-19 December 2000 Revised

CA-1500 SM 2-20 December 2000 Revised

CA-1500 SM 2-21 December 2000 Revised

2.8.2 Assembly Diagram

CA-1500 SM 2-22 December 2000 Revised

No. Sensor No. Sensor

CA-1500 SM 2-23 December 2000 Revised

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CA-1500 SM #2 Hydraulic&Mech

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